Penerapan Metode Demonstrasi Dan Tanya Jawab Dalam Meningkatkan Prestasi Belajar Kelas IV SD 4 Cendono Dawe Kudus

The purpose of this study is to describe the application of the demonstration method and question and answer in improving the learning achievement of class IV SD 4 Cranggang Dawe Kudus. This research is Classroom Action Research (CAR). The subjects of this study were in 4th grade Cranggang State Elementary School with a total of 25 students. In this action research using the form proposed by Kemmis and Taggart. The implementation of this research was carried out in 2 cycles, each of which went through four stages, namely the planning stage, the implementation stage, the data collection stage and the reflection stage. Data collection techniques in this study are observation, interviews, tests and questionnaires. The results of pre-cycle research, cycle I, and cycle II researchers know that there has been an increase in the acquisition of learning outcomes. The results of pre-cycle learning are the number of students who complete with a score above 70 as many as 11 students or only 44% of classical class completeness. In the improvement of learning in the first stage of the first cycle, the number of students who completed with grades above 70 were 15 students or only 60% of the classical class completeness. And in the improvement of learning in the second phase of cycle II, the number of students who completed with a score above 70 were 19 students, and 84% had achieved classical completion.Tujuan penelitian ini yaitu untuk mendreskripsikan penerapan metode demonstrasi dan tanya jawab dalam meningkatkan prestasi belajar kelas IV SD 4 Cranggang Dawe Kudus. Penelitian ini adalah Penelitian Tindakan Kelas (PTK). Subjek penelitian ini di Sekolah Dasar Negeri 4 Cranggang kelas IV dengan jumlah 25 siswa. Dalam penelitian tindakan ini menggunakan bentuk yang dikemukakan oleh Kemmis dan Taggart. Pelaksanaan penelitian ini dilaksanakan dalam 2 siklus, yang masing-masing melalui empat tahap yaitu tahap perencanaan, tahap pelaksanaan, tahap pengumpulan data dan tahap refleksi. Teknik pengumpulan data pada penelitian ini yaitu observasi, wawancara, tes dan angket. Hasil penelitian prasiklus, siklus I, dan siklus II peneliti ketahui bahwa telah terjadi peningkatan perolehan hasil belajar. Hasil belajar pra siklus jumlah siswa yang tuntas dengan nilai di atas 70 sejumlah 11 siswa atau hanya 44% ketuntasan klasikal kelas. Pada perbaikan pembelajaran tahap kesatu siklus I jumlah siswa yang tuntas dengan nilai di atas 70 sejumlah 15 siswa atau hanya 60% ketuntasan klasikal kelas. Dan pada perbaikan pembelajaran tahap kedua siklus II jumlah siswa yang tuntas dengan nilai di atas 70 sejunlah 19 siswa, dan sudah mencapai tuntas klasikal sebesar 84%.

Innate immune genes of the chicken MHC and related regions

Compared to the major histocompatibility complex (MHC) of typical mammals, the chicken BF/BL region is small and simple, with most of the genes playing central roles in the adaptive immune response. However, some genes of the chicken MHC are almost certainly involved in innate immunity, such as the complement component C4 and the lectin-like receptor/ligand gene pair BNK and Blec. The poorly expressed classical class I molecule BF1 is known to be recognised by natural killer (NK) cells and, analogous to mammalian immune responses, the classical class I molecules BF1 and BF2, the CD1 homologs and the butyrophilin homologs called BG may be recognised by adaptive immune lymphocytes with semi-invariant receptors in a so-called adaptate manner. Moreover, the TRIM and BG regions next to the chicken MHC, along with the genetically unlinked Y and olfactory/scavenger receptor regions on the same chromosome, have multigene families almost certainly involved in innate and adaptate responses. On this chicken microchromosome, the simplicity of the adaptive immune gene systems contrasts with the complexity of the gene systems potentially involved in innate immunity.

HLA-G1+ Expression in GGTA1KO Pigs Suppresses Human and Monkey Anti-Pig T, B and NK Cell Responses

The human leukocyte antigen G1 (HLA-G1), a non-classical class I major histocompatibility complex (MHC-I) protein, is a potent immunomodulatory molecule at the maternal/fetal interface and other environments to regulate the cellular immune response. We created GGTA1-/HLAG1+ pigs to explore their use as organ and cell donors that may extend xenograft survival and function in both preclinical nonhuman primate (NHP) models and future clinical trials. In the present study, HLA-G1 was expressed from the porcine ROSA26 locus by homology directed repair (HDR) mediated knock-in (KI) with simultaneous deletion of α-1-3-galactotransferase gene (GGTA1; GTKO) using the clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas9) gene-editing system. GTKO/HLAG1+ pigs showing immune inhibitory functions were generated through somatic cell nuclear transfer (SCNT). The presence of HLA-G1 at the ROSA26 locus and the deletion of GGTA1 were confirmed by next generation sequencing (NGS) and Sanger’s sequencing. Fibroblasts from piglets, biopsies from transplantable organs, and islets were positive for HLA-G1 expression by confocal microscopy, flow cytometry, or q-PCR. The expression of cell surface HLA-G1 molecule associated with endogenous β2-microglobulin (β2m) was confirmed by staining genetically engineered cells with fluorescently labeled recombinant ILT2 protein. Fibroblasts obtained from GTKO/HLAG1+ pigs were shown to modulate the immune response by lowering IFN-γ production by T cells and proliferation of CD4+ and CD8+ T cells, B cells and natural killer (NK) cells, as well as by augmenting phosphorylation of Src homology region 2 domain-containing phosphatase-2 (SHP-2), which plays a central role in immune suppression. Islets isolated from GTKO/HLA-G1+ genetically engineered pigs and transplanted into streptozotocin-diabetic nude mice restored normoglycemia, suggesting that the expression of HLA-G1 did not interfere with their ability to reverse diabetes. The findings presented here suggest that the HLA-G1+ transgene can be stably expressed from the ROSA26 locus of non-fetal maternal tissue at the cell surface. By providing an immunomodulatory signal, expression of HLA-G1+ may extend survival of porcine pancreatic islet and organ xenografts.

The Perception of Medical Students Towards Virtual Learning During Corona Pandemic at Faculties of Medicine at Alzaiem Alazhari and Khartoum Universities, Khartoum, 2021

Background: “Is COVID-19 pandemic the end of classical class room based education?” the most frequent question nowadays after the forced restrictions which included educational intuition lockdown، objectives, this paper aimed to study the perception of medical students towards virtual learning during COVID-19 pandemic at faculties of medicine at Alzaiem Alazhari and Khartoum universities in Sudan , 2021.Methods: This was a descriptive cross-sectional faculty -based study in which self-administered questionnaires were distributed to 357 medical students at faculties of medicine at Alzaiem Alazhari university and university of Khartoum. Respondents were selected using simple random sampling, and data was analyzed using SPSS 26. Results: This study revealed that the average number of hours that spent on virtual learning before and during the COVID-19 pandemic were statistically significant (p<0.05), with average of 1.9 hours per day before the pandemic and average of more than 3 hours per day during the pandemic (57.9%) .Moreover, the majority of the participants 77.9% (n=279) thought that virtual learning did not successfully replace the face to face classical teaching especially the clinical medical students 87.2% (n=312) who claimed that clinical skills cannot be taught virtually and they need the direct patient contact education in order to practice the clinical skills in a proper way.Conclusion: Virtual learning in these two medical faculties was found to be well perceived, because its pros outweighed its cons, as it was the most appropriate method to use in order to continue the education process during the pandemic.

Segregation Analysis of Genotyped and Family-Phased, Long Range MHC Classical Class I and Class II Haplotypes in 5 Families With Type 1 Diabetes Proband in the United Arab Emirates

The classical Human Leucocyte Antigen (HLA) class II haplotypes of the Major Histocompatibility Complex (MHC) that are associated with type 1 diabetes (T1D) were identified in five families from the United Arab Emirates (UAE). Segregation analyses were performed on these 5 families with the disease, 3 with one child and 2 with 2 children diagnosed with T1D. Three HLA-DR4 haplotypes were identified: HLA- DRB1∗04:01:01-DQB1∗03:02:01:01; HLA- DRB1∗04:02:01- DQB1∗03:02:01; and HLA -DRB1∗04:05:01-DQB1∗02:02:01:02. All have previously been identified to be associated with T1D in studies of the Arabian population. In the 10 parents from the 5 families, 9 had at least one HLA-DR4 and HLA-DR3 haplotype which potentially increases the risk of T1D. Of these 9 parents, 3 were heterozygous for HLA-DR4/HLA-DR3 and one was homozygous for HLA-DR3. Two haplotypes that were identified here extend to the HLA class I region were previously designated AH8.2 (HLA -A∗26-B∗08-DRB1∗03) and AH50.2 (HLA -C∗06-B∗50-DRB1∗03:01-DQ∗02) and associated with diabetes in neighboring North Indian populations. This study provides examples of MHC haplotype analysis in pedigrees to improve our understanding of the genetics of T1D in the understudied population of the UAE.

Evidence for a shift in placental HLA-G allelic dominance and the HLA-G isoform profile during a healthy pregnancy and pre-eclampsia

Human leukocyte antigen (HLA)-G is a non-classical class Ib major expressed by placental trophoblast cells plays a central role in establishing tolerance to the semi-allogeneic fetus and in placentation. HLA-G exists in different soluble or membrane-bound isoforms. Pre-eclampsia, a major cause of fetal and maternal morbidity and mortality, has been linked to insufficient placentation and an altered immune response in pregnancy, including altered HLA-G expression. The 14 bp insertion/deletion polymorphism in the 3′ untranslated region of the gene and the isoform profile may affect HLA-G expression. The aim of the current pilot study was to characterize the expression patterns of HLAG mRNA, protein and isoform profile in uncomplicated term pregnancies and in cases of pre-eclampsia. Maternal sHLA-G mRNA and protein levels was slightly reduced in pre-eclampsia. No difference was found for placental blood, and no correlation between peripheral and placental sHLA-G levels was found. We observed no association between neither fetal nor maternal HLA-G 14 bp insertion/deletion genotypes and pre-eclampsia, nor a significant difference in isoform profiles. However, in HLA-G 14 bp insertion/deletion heterozygous placental samples, we observed abundant HLA-G1 14 bp insertion allele expression in the term placentae, which is contrary to previous findings in first trimester trophoblast. Increased HLA-G1 14 bp insertion allele expression in the placenta was associated with reduced levels of placental sHLA-G and an altered isoform profile with increased relative levels of HLA-G1 and -G5 and reduced levels of HLA-G3. The results indicate that an allelic shift in heterozygous individuals could represent a novel regulatory pathway.

Identification of the Novel HLA-A*11:335 Allele, a Rare Interlocus Recombination Involving HLA-A*11:01:01:01 and HLA-H*02:07/14/18 Alleles With Nanopore Sequencing, in a Volunteer From the China Marrow Donor Program

Background: The major histocompatibility complex in humans includes three classical class I loci (A, B and C), which are important biomarkers for transplant of organs and hematopoietic stem cells. In the MHC, polymorphism is known to be extremely high while interlocus recombination is rare. We report a rare interlocus recombination between HLA-A and HLA-H, which was analyzed using next generation sequencing and nanopore sequencing. Results: In the sample, the genotypes of HLA-A, B, C, DRB1 and DQB1 were firstly phased with methods of sequence-specific primer, sequence-specific oligonucleotide, Sanger’s sequencing and NGS; however, HLA-A could not be phased. Nanopore sequencing was finally utilized to distinguish the sequence of the novel allele. Finally, the novel HLA-A*11:335 allele was identified as an interlocus recombination involving HLA-A*11:01:01:01 and HLA-H*02:07/14/18 alleles; this was mainly achieved by nanopore sequencing. Conclusions: The identification of the interlocus recombination indicated that nanopore sequencing may be the most precise method for HLA typing. Interlocus recombination has been identified as one of the mechanisms involved in the generation of novel HLA alleles.

Clinical Relevance of CD4 Cytotoxic T Cells in High-Risk Neuroblastoma

Neuroblastoma is the most common extracranial childhood solid tumor. The majority of high-risk neuroblastoma is resistant/refractory to the current high intensity therapy, and the survival of these patients remains poor for the last three decades. To effectively treat these extremely unfavorable neuroblastomas, innovative immunotherapy approaches would be the most promising. In this article, we discuss the identity of tumor-infiltrating effector cells and immunosuppressive cells in high-risk neuroblastoma. Neuroblastoma is unique in that it expresses little or no classical HLA Class I and II. In contrast, high-risk neuroblastomas express the stress-responsive non-classical Class I, HLA-E molecule. HLA-E is the ligand of activating receptors NKG2C/E that are expressed on memory NK cells, CD8+T cells and CD4 CTLs. By examining a comprehensive RNA-seq gene expression dataset, we detected relatively high levels of CD4 expression in high-risk neuroblastoma tissues. The majority of CD4+ cells were CD3+, and thus they were likely tumor-associated CD4+T cells. In addition, high-level of both CD4 and NKG2C/E expression was associated with prolonged survival of the high-risk neuroblastoma patients, but CD8 levels were not, further suggesting that the CD4+ NKG2C/E+ T cells or CD4 CTL conferred cytotoxicity against the neuroblastoma cells. However, this T cell mediated- “protective effect” declined over time, in part due to the progressive formation of immunosuppressive tumor microenvironment. These observations suggest that to improve survival of high-risk neuroblastoma patients, it is essential to gain insights into how to enhance CD4 CTL cytotoxicity and control the immunosuppressive tumor microenvironment during the course of the disease.

Injuries in Estonian professional ballet dancers in the 2019/2020 season

The aim of the study was to find the occurrence of musculoskeletal injuries in Estonia professional ballet dancers in the 2019/2020 season. A total of 62 dancers participated in the study, 25 were male and 37 female dancers. This study was a questionnaire-based, which was compiled on similar studies to collect the data among ballet dancers working in Estonia. The study showed that 58% of dancers were injured in the last 12 months. The most common type of dance injury during this period was muscle or tendon strain (33%), followed chronic inflammation (21%) and ankle sprain (20%). The most common injured body site was foot (20%), ankle (18%) and knee joint (10%). The highest number of injuries occurred during rehearsals (44%), classical class (27%), and during performances (16%). More than half of Estonian ballet dancers sustained at least one injury during the last twelve months. The most common types of injury were muscle or tendon strain, chronic inflammation and ankle sprain. The highest number of injuries occurred in the foot, whereas the highest number of injuries occurred during rehearsals.