scholarly journals Oral yeasts infection in children

2019 ◽  
Vol 14 (1) ◽  
pp. 7-15
Author(s):  
Niem N. Jammil ◽  
Manahil M. Yehia
Keyword(s):  
Oral Yeasts

scholarly journals The Use of MALDI-TOF Mass Spectrometry to Analyze Commensal Oral Yeasts in Nursing Home Residents

2021 ◽  
Vol 9 (1) ◽  
pp. 142
Author(s):  
Jang-Jih Lu ◽  
Hsiu-Jung Lo ◽  
Chih-Hua Lee ◽  
Mei-Jun Chen ◽  
Chih-Chao Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nursing Home ◽  
Dna Sequences ◽  
Nursing Home Residents ◽  
Practical Method ◽  
Elderly Subjects ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Oral Yeasts ◽  
Tof Ms

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate method to identify microorganisms in clinical laboratories. This study isolates yeast-like microorganisms in the oral washes that are collected from non-bedridden nursing home residents, using CHROMagar Candida plates, and identifies them using Bruker MALDI-TOF MS. The ribosomal DNA sequences of the isolates are then examined. Three hundred and twenty yeast isolates are isolated from the oral washes. Candida species form the majority (78.1%), followed by Trichosporon/Cutaneotrichosporon species (8.8%). Bruker MALDI-TOF MS gives a high-level confidence, with a log(score) value of ≥1.8, and identifies 96.9% of the isolates. There are six inconclusive results (1.9%), and those sequences are verified as rare clinical species, including Candida ethanolica, Cutaneotrichosporon jirovecii, Exophiala dermatitidis, and Fereydounia khargensis. Almost all of the isolates have a regular color on the CHROMagar Candida plates. If the colonies are grouped by color on the plates, a specific dominant yeast species is present in each color group, except for purple or orange isolates. In conclusion, MALDI-TOF MS is verified as a fast, accurate and practical method to analyze oral yeasts in elderly subjects.

Genetic relatedness of oral yeasts within and between patients with marginal periodontitis and subjects with oral health

2005 ◽  
Vol 40 (6) ◽  
pp. 446-452 ◽  
Author(s):  
Xiaobo Song ◽  
Emenike R. K. Eribe ◽  
Jinglu Sun ◽  
B. Frode Hansen ◽  
Ingar Olsen
Keyword(s):  
Oral Health ◽  
Genetic Relatedness ◽  
Oral Yeasts ◽  
Marginal Periodontitis

scholarly journals Detection of intracellular Helicobacter pylori in Candida. SPP from neonate oral swabs

2018 ◽  
Vol 64 (10) ◽  
pp. 928-935 ◽  
Author(s):  
Lillian Matamala-Valdés ◽  
Kimberly Sánchez-Alonzo ◽  
Cristian Parra ◽  
Katia Sáez ◽  
Alejandro Aguayo-Reyes ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Yeast Species ◽  
Identification System ◽  
Early Age ◽  
Age Group ◽  
Candida Spp ◽  
Cross Sectional ◽  
Oral Swab ◽  
H Pylori ◽  
Oral Yeasts

SUMMARY BACKGROUND: There is evidence of detection of Helicobacter pylori (H. pylori) in the stool of newborns and in the yeast that colonizes the oral cavity of this age group. However, there is a lack of research to confirm it. This study proposes to determine the existence of the bacteria at an early age, specifically in newborns. OBJECTIVE: To identify intracellular H. pylori in oral yeasts and to detect antigens of the bacteria in newborn stools. METHODOLOGY: Cross-sectional and descriptive study. Samples were obtained from infants (oral swab and meconium). Identification of yeast species was performed using the following techniques: CHROMagar Candida, Germinal Tube Test and API Candida Identification System, then the yeasts were observed by light microscopy and fluorescence. Detection of H. pylori antigen in meconium and PCR were performed to amplify specific genes of the bacterium (rRNA16S, cagA, vacA s1a, vacA s1b, vacA s2, vacA m1, vacA m2 and dupA). RESULTS: Intracellular H. pylori was detected in yeast of the species Candida glabrata (C. glabrata) isolated from an oral swab of a newborn. CONCLUSION: The results of this study evidenced the existence of intracellular H. pylori in newborns.

Oral yeasts in patients with cancer of the mouth, before and during radiotherapy

1990 ◽  
Vol 112 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Claudete R. Paula ◽  
Maria Carmeli C. Sampaio ◽  
Esther G. Birman ◽  
Antonio M. Siqueira
Keyword(s):  
Patients With Cancer ◽  
Oral Yeasts

scholarly journals Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

2004 ◽  
Vol 64 (2) ◽  
pp. 317-326 ◽  
Author(s):  
J. A. de O. Rodrigues ◽  
J. F. Höfling ◽  
F. C. A. Tavares ◽  
K. M. R. Duarte ◽  
R. B. Gonçalves ◽  
...  
Keyword(s):  
Candida Species ◽  
Profile Analysis ◽  
Protein Profile ◽  
Negative Control ◽  
Yeast Cells ◽  
Sds Page ◽  
Differential Medium ◽  
Oral Yeasts ◽  
Species Specific ◽  
High Level

The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies.

Oral yeasts and coliforms in HIV-infected individuals in Hong Kong

Mycoses ◽  
2000 ◽  
Vol 43 (7-8) ◽  
pp. 303-308 ◽  
Author(s):  
C. S. P. Tsang ◽  
L. P. Samaranayake
Keyword(s):  
Hong Kong ◽  
Oral Yeasts

scholarly journals Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

2001 ◽  
Vol 61 (3) ◽  
pp. 507-516 ◽  
Author(s):  
J. F. HÖFLING ◽  
E. A. R. ROSA ◽  
C. V. PEREIRA ◽  
M. F. G. BORIOLLO ◽  
J. A. O. RODRIGUES
Keyword(s):  
Numerical Analysis ◽  
Culture Media ◽  
Cell Protein ◽  
Buffer System ◽  
Whole Cell ◽  
Whole Cells ◽  
Yeast Strains ◽  
Sds Page ◽  
Oral Yeasts ◽  
Dimensional Electrophoresis

The application of gel electrophoresis and numerical analysis of yeast soluble proteins analysis to the investigation of 12 oral yeast strains belonging to five species is described. It involves one-dimensional electrophoresis of SDS-solubilized whole-cell proteins using different culture media for the cultivation of the cells, integration densitometries in the areas of the gels and percentages of the proteins extraction. These extracts were prepared from four isolates of Candida albicans, two of C. tropicalis, C. guilliermondii, C. parapsilosis and C. krusei. The extracts from whole-cells proteins using different culture media for the cultivation of the cells were fractionated by slab electrophoresis using a discontinuous buffer system. The corresponding patterns showed at least 36 polypeptides in the range of 14.4-200 kDa. Different isolates of each species were clearly different in each of the five species. The data obtained suggest that different nutritional compositions led to the expression of different proteins derived from alternatives metabolic pathways expressed by the electrophoretic profiles. The construction of a database of protein fingerprints and numerical analysis based on such data, may have some implications in the classification and identification of such species with epidemiological, ecological and taxonomic purposes. A well defined or synthetic culture media seems to be much properly.

scholarly journals Susceptibilities of Oral Bacteria and Yeast to Mammalian Cathelicidins

2001 ◽  
Vol 45 (11) ◽  
pp. 3216-3219 ◽  
Author(s):  
Janet M. Guthmiller ◽  
Kaaren G. Vargas ◽  
Rupasree Srikantha ◽  
Lori L. Schomberg ◽  
Paula L. Weistroffer ◽  
...  
Keyword(s):  
Fusobacterium Nucleatum ◽  
Oral Bacteria ◽  
Candida Krusei ◽  
Actinobacillus Actinomycetemcomitans ◽  
Future Studies ◽  
Oral Infections ◽  
Streptococcus Sanguis ◽  
Prevention And Treatment ◽  
Oral Yeasts ◽  
Potential Use

ABSTRACT The effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. We tested the susceptibilities of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum,Porphyromonas gingivalis, Streptococcus sanguis, Candida krusei, Candida tropicalis and Candida albicans to the following cathelicidins: FALL39, SMAP29, and CAP18. SMAP29 and CAP18 were antimicrobial, whereas FALL39 did not exhibit antimicrobial activity. Future studies are needed to determine the potential use of these antimicrobial peptides in prevention and treatment of oral infections.

The effect of storage in liquid nitrogen on the isolation of oral yeasts in human saliva

1992 ◽  
Vol 37 (3) ◽  
pp. 237-239 ◽  
Author(s):  
E. Brambilla ◽  
L. Strohmenger ◽  
G. Vogel
Keyword(s):  
Liquid Nitrogen ◽  
Human Saliva ◽  
Oral Yeasts
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