Effect of Grape Seeds Powder on the Level of Some Antioxidants and Lipid Peroxidation of White Male Albino Rats Exposed to Oxidative Stress

The aim of the present study was to investigate the protective effects of Sesbania grandiflora flower (SGF) extract on erythrocyte membrane in Streptozotocin (STZ)-induced diabetic rats. Adult male albino rats of Wistar strain, weighing 190–220 g, were made diabetic by an intraperitonial administration of STZ (45 mg/kg). Normal and diabetic rats were treated with SGF, and diabetic rats were also treated with glibenclamide as drug control, for 45 days. In this study plasma insulin and haemoglobin levels were decreased and blood glucose, glycosylated haemoglobin, protein oxidation, lipid peroxidation markers, and osmotic fragility levels were increased in diabetic rats. Moreover, erythrocytes antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxide, glutathione reductase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase activities and non-enzymatic antioxidants such as vitamin C, vitamin E, reduced glutathione (GSH), and oxidized glutathione (GSSG) levels were altered. Similarly, the activities of total ATPases, Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase were also decreased in the erythrocytes of diabetic rats. Administration of SGF to STZ-induced diabetic rats reduced blood glucose and glycosylated haemoglobin levels with increased levels of insulin and haemoglobin. Moreover, SGF reversed the protein and lipid peroxidation markers, osmotic fragility, membrane-bound ATPases activities, and antioxidant status in STZ-induced diabetic rats. These results suggest that SGF could provide a protective effect on diabetes by decreasing oxidative stress-associated diabetic complications.

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PROTECTIVE EFFECT OF GRAPE SEEDS EXTRACT AGAINST SODIUM NITRITE-INDUCED TOXICITY AND OXIDATIVE STRESS IN ALBINO RATS

Al-Azhar Journal of Pharmaceutical Sciences ◽

10.21608/ajps.2014.6956 ◽

2014 ◽

Vol 49 (1) ◽

pp. 1-34

Author(s):

Gihan Hammoud

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Sodium Nitrite ◽

Albino Rats ◽

Grape Seeds ◽

And Oxidative Stress

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Does Short-Term and Low-Dose N-Acetylcysteine Affect Oxidative Stress and Inflammation in The Liver Tissues of Diabetic Rats?

Biological Research For Nursing ◽

10.1177/10998004211003668 ◽

2021 ◽

pp. 109980042110036

Author(s):

Fatma Genç ◽

Emine Gülçeri GÜLEÇ Peker

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Proinflammatory Cytokines ◽

Liver Tissue ◽

Low Dose ◽

Biochemical Parameter ◽

Diabetic Rats ◽

Parameter Analysis ◽

Albino Rats ◽

Short Term

Diabetes mellitus is a serious chronic disease in which the oxidant-antioxidant balance is impaired, causing many complications, including hepatopathy. In this study, the effects of short-term and low-dose N-acetylcysteine (NAC) administration on the biochemical, proinflammatory, and oxidative stress parameters in the liver tissue of diabetic rats were investigated. Twenty-four adult male Wistar albino rats weighing approximately 250–300 g were divided into 4 groups (n = 6): Control, Streptozotosin (STZ)-induced diabetes (DM), NAC treatment (60 mg/kg), and STZ-induced diabetes treated with NAC (DM+NAC; 60 mg/kg). NAC treatment was administered intraperitoneally as a single daily dose for 7 days. At the end of the experiment (3 weeks), blood and liver samples were collected for biochemical parameter analysis. Lipid peroxidation, antioxidant parameters, and nitric oxide (NOx) levels were determined by spectrophotometric method. Tissue inflammation parameters were evaluated by ELISA. Lipid peroxidation, proinflammatory cytokines, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values increased significantly with diabetes. NAC treatment significantly decreased serum ALT and AST levels and proinflammatory cytokines in the diabetic group. Liver glutathione (GSH) and NOx levels increased significantly in the DM+NAC group ( p < 0.05). While NAC treatment reduced lipid peroxidation in the liver, it improved the inflammatory response and antioxidant status. The beneficial effect of NAC treatment may be due to its antioxidant activity and the resulting increased level of GSH. The results show that low-dose and short-term NAC treatment had a positive effect on oxidative damage and inflammation in liver tissue. NAC can be used as a potential antioxidant in diabetes to prevent hepatopathy.

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The Effect of Benniseed Oil (Sesamum indicum Linn) on Induced Hypercholesterolemia in Albino Rats

Journal of Advances in Biology & Biotechnology ◽

10.9734/jabb/2019/v21i130083 ◽

2019 ◽

pp. 1-7

Author(s):

Tomisin Karen Olasunkanmi ◽

Olubunmi Bolanle Ajayi ◽

Braimoh James

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Normal Control ◽

Seed Oil ◽

Sesamum Indicum ◽

Albino Rats ◽

Normal Rat ◽

Group 2 ◽

State Group ◽

Hepatic Stress

The study was designed to investigate the antioxidant and antitoxicological potential of Sesamum indicum Linn seed (benni seed) oil on hypercholesterolemic rat. Albino rats weighing between 120-130 g were divided into two groups, group 1, was fed with normal rat diet(normal control), groups 2 was fed 1% cholesterol and 20% soya bean oil for 3 weeks to induce hypercholesterolemic state. Group 2 was later divided into groups 2, 3 and 4, group 2 was untreated, groups 3 and 4 were later fed with 5% and 10% Sesamum indicum L. seed oil incorporated in normal rat diet for another 6 weeks respectively. Significant (P<0.05) increase in lipid peroxidation (TBARs) and reduction in superoxide dismutate (SOD) and catalase (CAT) was observed in the liver of the hypercholesterolemic rats as compared to the normal control. At the same time, the oxidative stress causes significant (P<0.05) increase in serum level of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) of hypercholesterolemic rats. Administering Sesamum indicum Linn seed oil significantly reduced (P<0.05), serum ALT, AST, ALP and lipid peroxidation, elevated the level of SOD and CAT in the liver of Sesamum oil treated hypercholesterolemic rats. These findings indicate that Sesamum indicum Linn seed oil show possible prevention of hepatic stress by high cholesterol and free radical mediated oxidative stress in cells of experimental hypercholesterolemic rats.

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Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

Archives of Biological Sciences ◽

10.2298/abs1001075t ◽

2010 ◽

Vol 62 (1) ◽

pp. 75-82 ◽

Cited By ~ 1

Author(s):

Ivana Trbojevic ◽

Branka Ognjanovic ◽

Natasa Djordjevic ◽

Snezana Markovic ◽

A.S. Stajn ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Membrane Lipids ◽

Redox Status ◽

Protective Role ◽

Male Rats ◽

Albino Rats ◽

Wistar Albino Rats ◽

Glutathione Redox

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.

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Phytic Acid Exposure Alters AflatoxinB1-Induced Reproductive and Oxidative Toxicity in Albino Rats (Rattus norvegicus)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem137 ◽

2009 ◽

Vol 6 (3) ◽

pp. 331-341 ◽

Cited By ~ 11

Author(s):

Abdelaziz S. Abu El-Saad ◽

Hamada M. Mahmoud

Keyword(s):

Lipid Peroxidation ◽

Phytic Acid ◽

Rattus Norvegicus ◽

White Male ◽

Relative Weight ◽

Mitotic Division ◽

Control Group ◽

Albino Rats ◽

Lipid Peroxidation Product ◽

Sterile Phosphate Buffer

The increased use of feed in Egypt's aquaculture and animal industries raises concerns about the possible presence of mycotoxins in feedstuffs. The use of alternative medicine, such as botanicals and nutritional supplements, has become popular with inflammatory cases. The present study aimed to testify the role played by phytic acid (IP6) in enhancing the reproductive and oxidative toxicity induced in aflatoxinB1 (AFB1) treated white male albino rats (Rattus norvegicus) throughout treatment and withdrawal periods. One hundred and twenty white male albino rats were grouped into four groups. Group 1, was injected with 300 μg kg−1body wt of AFB1 once every 3 days for 15 days and left uninjected for another 15 days to study the withdrawal effect. Group 2, was injected with 300 μg kg−1body wt of AFB1 once every 3 days for 15 days and treated simultaneously with IP6 daily for another 15 days. Group 3, was treated daily with IP6 (40 mg kg−1body wt) for 15 days and with no treatment for other 15 days. Group 4, injected with equivalent volume of sterile phosphate buffer saline solution as a control group. Sera were taken at the experimental intervals and assayed for testosterone hormone, follicular-stimulating hormone (FSH) and luteinizing hormone (LH) to determine the toxicological impact of AFB1 and the possibility of amelioration by phytic acid on the reproductive performance of the studied animal. The effects of AFB1 treatment on the absolute and relative weight of testis as well as its histopathologic effect on the testis and the possibility of amelioration by IP6 treatment were evaluated. The activities of enzymatic and non-enzymatic anti-oxidants, in addition to lipid peroxidation were measured in the testis’ homogenate of AFB1-treated rats. A decrease in sex hormone levels, an increase in testicular lipid peroxidation product levels and a significant decrease in testicular glutathione content, catalase and total peroxidase and superoxide dismutase activities were recorded. The histopathologic alterations revealed a degeneration and highly mitotic division within the spermatogenic nuclei, in addition to some karyomegaly and nuclear pyknosis. It is concluded that the reduction in the toxicity of free radicals by phytic acid might be responsible for the protective influence observed.

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Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats

Turkish Journal of Biochemistry ◽

10.1515/tjb-2016-0211 ◽

2017 ◽

Vol 42 (4) ◽

Author(s):

Mahgoub Mohamed Ahmed

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Oxidative Damage ◽

Protective Effect ◽

Rat Liver ◽

Adult Male ◽

Total Protein ◽

Albino Rats ◽

Serum Total Protein

AbstractObjective:The objective of the current study is to investigate the protective effect ofMethods:Forty adult male albino rats were divided into four groups as control,Results:The results showed that, administration of EtOH caused a significant decrease (p<0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.Conclusion:had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver.

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Evaluation of Stress Enzymes Activities and Lipid Peroxidation in Heart Homogenates of Male Albino Rats Following the Administration of Diclofenac

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2020/v6i330117 ◽

2020 ◽

pp. 10-16

Author(s):

Felicia Nmeazi Okwakpam ◽

S. Abarikwu ◽

M. O. Monanu

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Reactive Oxygen ◽

Redox Status ◽

Male Rats ◽

Albino Rats ◽

Feed Water ◽

Oxygen Species ◽

Stress Enzymes

Nonsteroidal anti-inflammatory drugs are associated with an increase in cardiovascular events despite its uses in the therapeutic agent for the management of long- and short-term pain. Over the last years, evidence has accumulated showing that oxidative stress plays an important role in the pathogenesis of cardiovascular diseases. Oxidative stress is no longer considered as a simple imbalance between the production and scavenging of reactive oxygen species (ROS), but as a dysfunction of enzymes involved in ROS production. This study investigated the effect of diclofenac on the activity of oxidative stress enzymes as well as formation of lipid peroxidation. Male rats weighing about 100-120 g were divided into four groups: group one (control, feed+water) group two, group three and group four treated with different mg/kg/day of drugs (50 mg/kg/day, 100 mg/kg/day and 150 mg/kg/day) feed and water respectively for 7 days. Analysis on the effect of diclofenac on the activities of stress enzymes such as nicotine adenosine dinucleotide phosphate hydrogenase oxidase (NADPHoxidase), xanthine oxidase(XOD), catalase(CAT), superoxide dismutase(SOD) and Glutathione Peroxidase as well as evaluation of lipid peroxidation by measuring malondialdehyde (MDA) in the heart homogenate were carried out and the result showed a significant increase in each parameter given rise to the production of reactive oxygen species (ROS) if not moderated by the antioxidant defense can lead to cardiac impairment as a result of oxidative stress damage or injury. The result obtained implies that diclofenac (NSAIDs) affects the redox status of vascular tissues (heart tissues).

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