Efficiency of Salicornia neei to Treat Aquaculture Effluent from a Hypersaline and Artificial Wetland

In this study, we evaluated the potential of Salicornia neei, a halophyte plant native to South America, to treat saline effluents with simulated concentration of ammonium-N (Amm) and nitrate-N (Nit) in a similar manner to land-based marine aquaculture effluents. Plants were cultivated for 74 days in drainage lysimeters under three treatments of seawater fertilized with: (1) Nit + Amm, (2) Nit, or (3) without fertilizer (Control). Over five repetitions, nitrogen removal efficiency (RE) was high in both treatments (Nit + Amm = 89.6% ± 1.0%; Nit 88.8% ± 0.9%), whereas the nitrogen removal rate (RR) was nonlinear and concentration-dependent (RRday1–4: Nit + Amm = 2.9 ± 0.3 mg L−1 d−1, Nit = 2.4 ± 0.5 mg L−1 d−1; RRday5–8: Nit + Amm = 0.8 ± 0.2 mg L−1 d−1, Nit = 1.0 ± 0.2 mg L−1 d−1). Effluent salinity increased from 40.6 to 49.4 g L−1 during the experiment, with no observed detrimental effects on RE or RR. High nitrogen removal efficiency and significant biomass production were observed (Nit + Amm = 11.3 ± 2.0 kg m−2; Nit = 10.0 ± 0.8 kg m−2; Control = 4.6 ± 0.6 kg m−2) demonstrate that artificial wetlands of S. neei can be used for wastewater treatment in saline aquaculture in South America.

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Efficiency of Salicornia neei to treat aquaculture effluent from a hypersaline and artificial wetland

10.1101/2020.09.06.259358 ◽

2020 ◽

Author(s):

Mónica R. Diaz ◽

Javier Araneda ◽

Andrea Osses ◽

Jaime Orellana ◽

José A. Gallardo

Keyword(s):

South America ◽

Removal Efficiency ◽

Nitrogen Removal ◽

Removal Rate ◽

High Nitrogen ◽

Artificial Wetlands ◽

Marine Aquaculture ◽

Nitrate N ◽

Ammonium N ◽

Simulated Concentration

AbstractIn this study we evaluated the potential of Salicornia neei, a halophyte plant native to South America, to treat saline effluents with simulated concentration of ammonium-N (Amm) and nitrate-N (Nit) similar to land-based marine aquaculture effluents. Plants were cultivated for 74 days in drainage lysimeters under three treatments of seawater fertilized with: 1) Nit+Amm, 2) Nit, or 3) without fertilizer (Control). Over 5 repetitions, nitrogen removal efficiency (RE) was high in both treatments (Nit + Amm = 89.6± 1,0 %; Nit 88.8 ± 0.9 %). While nitrogen removal rate (RR) was non linear and concentration-dependent (RRday 1-4: Nit+Amm= 2.9 ± 0.3 mg L−1 d−1, Nit = 2.4 ± 0.5mg L−1 d−1; RRday5-8: Nit + Amm = 0.8 ± 0.2mg L−1 d−1, Nit=1.0 ± 0.2mg L−1 d−1). Effluent salinity increased from 40.6 to 49.4 g L−1 during the experiment, with no observed detrimental effects on RE or RR. High nitrogen removal efficiency and significant biomass production observed, Nit+Amm = 11.3 ± 2.0 kg m−2; Nit = 10.0 ± 0.8 kg m−2; Control = 4.6 ± 0.6 kg m−2, demonstrate that artificial wetlands of S. neei can be used for wastewater treatment in saline aquaculture in South America.

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Study on De-Nitrification of Improved Step-Feed Progress

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.703.171 ◽

2014 ◽

Vol 703 ◽

pp. 171-174

Author(s):

Bing Wang ◽

Yi Xiao ◽

Shou Hui Tong ◽

Lan Fang ◽

Da Hai You ◽

...

Keyword(s):

Water Quality ◽

Organic Matter ◽

Fluidized Bed ◽

Removal Efficiency ◽

Nitrogen Removal ◽

Removal Rate ◽

Operating Conditions ◽

Removal Mechanism ◽

Aerobic Zone

Improved step-feed de-nitrification progress combined with biological fluidized bed was introduced in this study. The progress had good performance and capacity of de-nitrification and organic matter. The experiment result showed that the de-nitrification efficiency of the improved biological fluidized bed with step-feed process was higher than the fluidized bed A/O process under the same water quality and the operating conditions. When the influent proportion of each segment was equal, the system showed good nitrogen removal efficiency with the change of influent C/N ratio, HRT and sludge return ratio. The removal rate of TN reached up to 88.2%. It showed that the simultaneous nitrification and de-nitrification phenomenon happened in the aerobic zone. The nitrogen removal mechanism was also studied.

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Evaluation of a novel low-carbon to nitrogen- and temperature-tolerant simultaneously nitrifying–denitrifying bacterium and its use in the treatment of river water

RSC Advances ◽

10.1039/c8ra04697b ◽

2018 ◽

Vol 8 (48) ◽

pp. 27417-27422 ◽

Cited By ~ 4

Author(s):

Peng Jin ◽

Yinyan Chen ◽

Zhanwang Zheng ◽

Qizhen Du

Keyword(s):

Low Temperature ◽

River Water ◽

Removal Efficiency ◽

Nitrogen Removal ◽

Simultaneous Nitrification And Denitrification ◽

Low Carbon ◽

High Nitrogen ◽

Denitrifying Bacterium ◽

Nitrification And Denitrification

A novel simultaneous nitrification and denitrification Klebsiella sp. exhibits high nitrogen removal efficiency under low-temperature and low C/N wastewater.

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Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

Water Science & Technology ◽

10.2166/wst.2018.322 ◽

2018 ◽

Vol 78 (9) ◽

pp. 1843-1851 ◽

Cited By ~ 3

Author(s):

İ. Çelen-Erdem ◽

E. S. Kurt ◽

B. Bozçelik ◽

B. Çallı

Keyword(s):

Removal Efficiency ◽

Nitrogen Removal ◽

Municipal Wastewater ◽

Removal Rate ◽

Treatment Plant ◽

Packed Bed ◽

Municipal Wastewater Treatment ◽

Two Stage ◽

N Removal ◽

Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

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High nitrogen removal rate using ANAMMOX process at short hydraulic retention time

Water Science & Technology ◽

10.2166/wst.2013.641 ◽

2013 ◽

Vol 67 (5) ◽

pp. 968-975 ◽

Cited By ~ 9

Author(s):

C. G. Casagrande ◽

A. Kunz ◽

M. C. De Prá ◽

C. R. Bressan ◽

H. M. Soares

Keyword(s):

Nitrogen Removal ◽

Removal Rate ◽

Uasb Reactor ◽

Anaerobic Sludge ◽

Ammonium Oxidation ◽

High Nitrogen ◽

Total N ◽

Column Reactor ◽

Anaerobic Sludge Blanket ◽

Anammox Process

The anaerobic ammonium oxidation (ANAMMOX) is a chemolithoautotrophic process, which converts NH4+ to N2 using nitrite (NO2−) as the electron acceptor. This process has very high nitrogen removal rates (NRRs) and is an alternative to classical nitrification/denitrification wastewater treatment. In the present work, a strategy for nitrogen removal using ANAMMOX process was tested evaluating their performance when submitted to high loading rates and very short hydraulic retention times (HRTs). An up-flow ANAMMOX column reactor was inoculated with 30% biomass (v v−1) fed from 100 to 200 mg L−1 of total N (NO2−-N + NH4+-N) at 35 °C. After start-up and process stability the maximum NRR in the up-flow anaerobic sludge blanket (UASB) reactor was 18.3 g-N L−1 d−1 operated at 0.2 h of HRT. FISH (fluorescence in situ hybridization) analysis and process stoichiometry confirmed that ANAMMOX was the prevalent process for nitrogen removal during the experiments. The results point out that high NRRs can be obtained at very short HRTs using up-flow ANAMMOX column reactor configuration.

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Effects of Hydraulic Retention Time and Influent Nitrate-N Concentration on Nitrogen Removal and the Microbial Community of an Aerobic Denitrification Reactor Treating Recirculating Marine Aquaculture System Effluent

Water ◽

10.3390/w12030650 ◽

2020 ◽

Vol 12 (3) ◽

pp. 650 ◽

Cited By ~ 1

Author(s):

Xiefa Song ◽

Xiaohan Yang ◽

Eric Hallerman ◽

Yuli Jiang ◽

Zhitao Huang

Keyword(s):

Microbial Community ◽

Nitrogen Removal ◽

Retention Time ◽

Hydraulic Retention Time ◽

Aerobic Denitrification ◽

Marine Aquaculture ◽

Nitrate N ◽

Aquaculture System ◽

N Concentration ◽

Denitrification Reactor

The effects of hydraulic retention time (HRT) and influent nitrate-N concentration on nitrogen removal and the microbial community composition of an aerobic denitrification reactor treating recirculating marine aquaculture system effluent were evaluated. Results showed that over 98% of nitrogen was removed and ammonia-N and nitrite-N levels were below 1 mg/L when influent nitrate-N was below 150 mg/L and HRT over 5 h. The maximum nitrogen removal efficiency and nitrogen removal rate were observed at HRT of 6 or 7 h when influent nitrate-N was 150 mg/L. High-throughput DNA sequencing analysis revealed that the microbial phyla Proteobacteria and Bacteroidetes were predominant in the reactor, with an average relative total abundance above 70%. The relative abundance of denitrifying bacteria of genera Halomonas and Denitratisoma within the reactor decreased with increasing influent nitrate-N concentrations. Our results show the presence of an aerobically denitrifying microbial consortium with both expected and unexpected members, many of them relatively new to science. Our findings provide insights into the biological workings and inform the design and operation of denitrifying reactors for marine aquaculture systems.

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Nitrogen removal efficiency and microbial community analysis of ANAMMOX biofilter at ambient temperature

Water Science & Technology ◽

10.2166/wst.2015.019 ◽

2015 ◽

Vol 71 (5) ◽

pp. 725-733 ◽

Cited By ~ 8

Author(s):

Zeng Taotao ◽

Li Dong ◽

Zeng Huiping ◽

Xie Shuibo ◽

Qiu Wenxin ◽

...

Keyword(s):

Microbial Community ◽

Ambient Temperature ◽

Removal Efficiency ◽

Nitrogen Removal ◽

Denaturing Gradient Gel Electrophoresis ◽

Removal Rate ◽

Ammonia Concentration ◽

Visual Observation ◽

Microbial Community Analysis ◽

Anammox Bacteria

An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3–23.2 °C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m3 day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.

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The effect of aerated rock filter geometry on the rate of nitrogen removal from facultative pond effluents

Water Science & Technology ◽

10.2166/wst.2011.102 ◽

2011 ◽

Vol 63 (5) ◽

pp. 841-844 ◽

Cited By ~ 2

Author(s):

R. Hamdan ◽

D. D. Mara

Keyword(s):

Nitrogen Removal ◽

Ammonium Nitrogen ◽

Horizontal Flow ◽

Vertical Flow ◽

Stabilization Pond ◽

Waste Stabilization Pond ◽

Nitrate N ◽

N Removal ◽

Ammonium N ◽

Waste Stabilization

Rock filters are an established technology for polishing waste stabilization pond effluents. However, they rapidly become anoxic and consequently do not remove ammonium-nitrogen. Horizontal-flow aerated rock filters (HFARF), developed to permit nitrification and hence ammonium-N removal, were compared with a novel vertical-flow aerated rock filter (VFARF). There were no differences in the removals of BOD5, TSS and TKN, but the VFARF consistently produced effluents with lower ammonium-N concentrations (<0.3 mg N/L) than the HFARF (0.8−1.5 mg N/L) and higher nitrate-N concentrations (24–29 mg N/L vs. 17–24 mg N/L).

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Effect of Fe on Nitrogen Removal Rate and Microbes of Anammox

E3S Web of Conferences ◽

10.1051/e3sconf/202127104006 ◽

2021 ◽

Vol 271 ◽

pp. 04006

Author(s):

Bing Wang ◽

Bing Sun ◽

Yunlong Liu ◽

Lin Yang

Keyword(s):

Removal Efficiency ◽

Nitrogen Removal ◽

Positive Impact ◽

Removal Rate ◽

Positive Effect

With the anammox sludge as the inoculation sludge, the effect of different concentrations of Fe2+ and Fe3+ on the denitrification performance of the anammox reaction was explored by setting a control experiment.The results showed that when the Fe2+ concentration was 0.08mmol/l, the reactor had the best removal efficiency, the removal rate of NH4+-N was 89.14% after 60h, and the removal rate of NO2--N was 85.9%. The positive effect of Fe3+ on the anammox reaction was similar to that of Fe2+. From reading the literature, it can be known that Fe has a positive impact on anammox microorganisms in three aspects by promoting microbial enrichment, promoting the production of functional enzymes, and promoting microbial granulation.

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