scholarly journals Effect of Fe on Nitrogen Removal Rate and Microbes of Anammox

2021 ◽  
Vol 271 ◽  
pp. 04006
Author(s):  
Bing Wang ◽  
Bing Sun ◽  
Yunlong Liu ◽  
Lin Yang

With the anammox sludge as the inoculation sludge, the effect of different concentrations of Fe2+ and Fe3+ on the denitrification performance of the anammox reaction was explored by setting a control experiment.The results showed that when the Fe2+ concentration was 0.08mmol/l, the reactor had the best removal efficiency, the removal rate of NH4+-N was 89.14% after 60h, and the removal rate of NO2--N was 85.9%. The positive effect of Fe3+ on the anammox reaction was similar to that of Fe2+. From reading the literature, it can be known that Fe has a positive impact on anammox microorganisms in three aspects by promoting microbial enrichment, promoting the production of functional enzymes, and promoting microbial granulation.

2014 ◽  
Vol 703 ◽  
pp. 171-174
Author(s):  
Bing Wang ◽  
Yi Xiao ◽  
Shou Hui Tong ◽  
Lan Fang ◽  
Da Hai You ◽  
...  

Improved step-feed de-nitrification progress combined with biological fluidized bed was introduced in this study. The progress had good performance and capacity of de-nitrification and organic matter. The experiment result showed that the de-nitrification efficiency of the improved biological fluidized bed with step-feed process was higher than the fluidized bed A/O process under the same water quality and the operating conditions. When the influent proportion of each segment was equal, the system showed good nitrogen removal efficiency with the change of influent C/N ratio, HRT and sludge return ratio. The removal rate of TN reached up to 88.2%. It showed that the simultaneous nitrification and de-nitrification phenomenon happened in the aerobic zone. The nitrogen removal mechanism was also studied.


2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.


2015 ◽  
Vol 71 (5) ◽  
pp. 725-733 ◽  
Author(s):  
Zeng Taotao ◽  
Li Dong ◽  
Zeng Huiping ◽  
Xie Shuibo ◽  
Qiu Wenxin ◽  
...  

An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3–23.2 °C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m3 day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.


Agriculture ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 621
Author(s):  
Mónica R. Diaz ◽  
Javier Araneda ◽  
Andrea Osses ◽  
Jaime Orellana ◽  
José A. Gallardo

In this study, we evaluated the potential of Salicornia neei, a halophyte plant native to South America, to treat saline effluents with simulated concentration of ammonium-N (Amm) and nitrate-N (Nit) in a similar manner to land-based marine aquaculture effluents. Plants were cultivated for 74 days in drainage lysimeters under three treatments of seawater fertilized with: (1) Nit + Amm, (2) Nit, or (3) without fertilizer (Control). Over five repetitions, nitrogen removal efficiency (RE) was high in both treatments (Nit + Amm = 89.6% ± 1.0%; Nit 88.8% ± 0.9%), whereas the nitrogen removal rate (RR) was nonlinear and concentration-dependent (RRday1–4: Nit + Amm = 2.9 ± 0.3 mg L−1 d−1, Nit = 2.4 ± 0.5 mg L−1 d−1; RRday5–8: Nit + Amm = 0.8 ± 0.2 mg L−1 d−1, Nit = 1.0 ± 0.2 mg L−1 d−1). Effluent salinity increased from 40.6 to 49.4 g L−1 during the experiment, with no observed detrimental effects on RE or RR. High nitrogen removal efficiency and significant biomass production were observed (Nit + Amm = 11.3 ± 2.0 kg m−2; Nit = 10.0 ± 0.8 kg m−2; Control = 4.6 ± 0.6 kg m−2) demonstrate that artificial wetlands of S. neei can be used for wastewater treatment in saline aquaculture in South America.


2020 ◽  
Author(s):  
Mónica R. Diaz ◽  
Javier Araneda ◽  
Andrea Osses ◽  
Jaime Orellana ◽  
José A. Gallardo

AbstractIn this study we evaluated the potential of Salicornia neei, a halophyte plant native to South America, to treat saline effluents with simulated concentration of ammonium-N (Amm) and nitrate-N (Nit) similar to land-based marine aquaculture effluents. Plants were cultivated for 74 days in drainage lysimeters under three treatments of seawater fertilized with: 1) Nit+Amm, 2) Nit, or 3) without fertilizer (Control). Over 5 repetitions, nitrogen removal efficiency (RE) was high in both treatments (Nit + Amm = 89.6± 1,0 %; Nit 88.8 ± 0.9 %). While nitrogen removal rate (RR) was non linear and concentration-dependent (RRday 1-4: Nit+Amm= 2.9 ± 0.3 mg L−1 d−1, Nit = 2.4 ± 0.5mg L−1 d−1; RRday5-8: Nit + Amm = 0.8 ± 0.2mg L−1 d−1, Nit=1.0 ± 0.2mg L−1 d−1). Effluent salinity increased from 40.6 to 49.4 g L−1 during the experiment, with no observed detrimental effects on RE or RR. High nitrogen removal efficiency and significant biomass production observed, Nit+Amm = 11.3 ± 2.0 kg m−2; Nit = 10.0 ± 0.8 kg m−2; Control = 4.6 ± 0.6 kg m−2, demonstrate that artificial wetlands of S. neei can be used for wastewater treatment in saline aquaculture in South America.


Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1832
Author(s):  
Jie Xu ◽  
Chao Zhu ◽  
Yi Liu ◽  
Guanghui Lv ◽  
Changyan Tian ◽  
...  

Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.


2020 ◽  
Vol 34 (3) ◽  
pp. 193-207
Author(s):  
Wen Zhang ◽  
Zhen Zhang ◽  
Sufeng Wang

How to simplify the nitrogen removal process, reduce the cost and improve the efficiency has become an urgent problem to be solved. In this research, the isolated HNAD (heterotrophic nitrification and aerobic denitrification) bacteria were used to remove<br /> the nitrogen in wastewater. Modified absorbent stone was used as high-efficiency and<br /> low-cost immobilized material. The modification effect was determined by the changes<br /> in mechanical strength, Zeta potential, pore structure, micrographs and biomass. The<br /> practicability of the modified carrier was further proved by experiments of environmental effect and reuse. The modified carrier had excellent performance. By comparing the<br /> degradation effects of immobilized microorganism and free microorganism, it was proved<br /> that the immobilized microorganisms have broad application prospects and strong adaptability to environmental factors. Under the optimum conditions (temperature of 30 oC,<br /> pH of 7, dissolved oxygen of 3.5 mg L–1), the removal efficiency of ammonia nitrogen<br /> reached 100 % in 40 hours, the removal efficiency of total nitrogen reached 60.11 % in<br /> 50 hours, and the removal rate of total nitrogen was 2.404 mg-NL–1 h–1 by immobilized<br /> microorganisms with the treatment of simulated nitrogen-containing wastewater. This<br /> research provides new material for the immobilization of HN-AD bacteria and a new way for nitrogen removal.


2021 ◽  
Vol 261 ◽  
pp. 02011
Author(s):  
Cai Qing ◽  
Ding Jiajia

Two parallel CANON Sequencing Bach reactors were started, and 67ppm NO2 was added into Sequencing Bach Reactor 2 while nothing was added to Sequencing Bach Reactor 1. The total nitrogen removal efficiency of SBR1 was 65.5±5.0% at a removal rate of 0.198±0.023 kgN/m3/d. Meanwhile, the SBR2 with NO2 addition showed a removal efficiency of 67.5±6.2%, with a removal rate of 0.277±0.017 kgN/m3/d. The SBR2 had a higher removal efficiency and rate than the SBR1. The continuous addition of trace NO2 into the CANON Sequencing Bach Reactor allows conventional aerobic ammonia oxidation with O2 as the electron acceptor and ammonia oxidation of with NO2 as the electron acceptor to take place simultaneously, thus improving the ammonia oxidation rate and autotrophic nitrogen removal performance. China Library Classification No.: X703.1 Literature Label: A Article No.:


2011 ◽  
Vol 183-185 ◽  
pp. 1114-1117
Author(s):  
Jin Long Zuo ◽  
Zhi Wei Zhao

Nowadays the sauce wastewater is doing greater harm to the water environment in China. In order to tackle this problem, the temperature influence on the sauce wastewater treatment effect was investigated. The results showed that when the temperature is 22~32°C the ammonia nitrogen removal effect was good and the ammonia nitrogen removal rate could reach more than 90%. The temperature had no obvious influence on nitrate for sauce wastewater. When the temperature is 22~32°C, the effluent orthophosphate concentration could reach about 1mg/L and the removal efficiency could reach about 70% with aeration time 2.5 h. Thus the sauce wastewater removal effect could be influenced by the temperature.


2012 ◽  
Vol 518-523 ◽  
pp. 473-477
Author(s):  
Xia Zhao ◽  
Hui Xia Feng ◽  
Feng Jiang ◽  
Na Li Chen ◽  
Xiao Chun Wang

In sequencing batch reactor aerobic granular sludge was cultivated, and the influence of influent C/N ratio to aerobic granular sludge was studied. The results showed that the granulation and the settling ability of the sludge were poor in high C/N, however, low C/N was beneficial to the accumulation of microorganism in reactor and MLSS could reach to as high as 8740 mg/L. Lower C/N ratio would lead to increase of particle size and disintegrate of loose structure and overgrowth on filamentous microbe, these were disadvantage of the stability of the system. It was not obvious that influent C/N ratio affected on the organic removal. The COD removal maintained at 87% after the preliminary form particles were formed in reactor. When C/N ratio was 100:15~100:35, the phosphorus removal efficiency was good. If C/N ratio was too high or too low, the formation of sludge granulation would be affected in the process. The influence of C/N ratio to ammonia nitrogen removal efficiency was obvious. While C/N ratio was 100:10, granular sludge had good simultaneous nitrification and denitrification performance, and the average removal of ammonia nitrogen attained to 91%. But low C/N ratio was able to inhibit the activity of nitrifying bacteria and denitrifying bacteria. At that time, ammonia nitrogen removal rate declined sharply in the system.


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