scholarly journals Inoculation with Mycorrhizal Fungi and Other Microbes to Improve the Morpho-Physiological and Floral Traits of Gazania rigens (L.) Gaertn

Agriculture ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 51 ◽  
Author(s):  
Ishan Saini ◽  
Ashok Aggarwal ◽  
Prashant Kaushik
Keyword(s):  
Mycorrhizal Fungi ◽  
Block Design ◽  
Combined Treatment ◽  
Trichoderma Viride ◽  
Growth Yield ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Microbial Inoculants ◽  
Acaulospora Laevis ◽  
Flower Quality

In the present analysis, we evaluated the effect of different microbial inoculants on growth and floral responses of Gazania rigens (L.) Gaertn. Two prevailing arbuscular mycorrhizal fungi (AMF) i.e., Funelliformis mosseae and Acaulospora laevis, along with Trichoderma viride and Pseudomonas fluorescens, were examined in a pot experiment. Independent roles of these four microbes and their different combinations were used in sixteen treatments of G. rigens. The experiment was conducted in a polyhouse with five replicates per treatment in a randomized complete block design. The results of microbial inoculants were very effective for growth yield and flowering response over the control. Early flowering was noted in the combination of F. mosseae, A. laevis and P. fluorescens, which also showed the best results for flower head size, flower fresh and dry weight, total chlorophyll, carotene and phosphorus content. Arbuscular mycorrhiza (AM) root colonization (%) and AM spore number were also the highest for the same treatment, whereas the number of leaves, number of buds and root length were maximum in the combined treatment (F. mosseae + A. laevis + T. viride + P. fluorescens). Overall, this study proposes that growers should consider microbial inoculants for the better yield and flower quality of G. rigens.

scholarly journals Influence of Biostimulants on Important Traits ofZinnia elegansJacq. under Open Field Conditions

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ishan Saini ◽  
Ashok Aggarwal ◽  
Prashant Kaushik
Keyword(s):  
Open Field ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Trichoderma Viride ◽  
Growth Yield ◽  
Arbuscular Mycorrhizal ◽  
Field Trials ◽  
Morphological Characters ◽  
Field Conditions ◽  
Flower Quality

The efficacy of microbial treatment on growth, yield, and nutrient uptake is very well acknowledged for field crops. However, the use of microbes forZinnia eleganshas rarely been exploited under field trials. Therefore, in this study, we have evaluated the efficacy of different microbial bioinoculants on sixteen morphological and nine biochemical traits ofZ.elegans. We used two arbuscular mycorrhizal fungi (AMF) (Glomus mosseae(G) andAcaulospora laevis(A)) along withTrichoderma viride(T) andPseudomonas florescence(P) as five different treatments under open field conditions, in a randomized complete block design. There were significant differences for all of the traits studied. Treatment 5 (G + A + T + P) was noted as the best treatment for the improvement of morphological characters, whereas Treatment 4 (G + A + P) was most significant for the biochemical trait improvement inZ. elegans. Overall, this study provides useful insight into the bioinoculant treatment that can be applied to improve the yield and flower quality ofZ.elegansunder open field conditions.

scholarly journals Growth Response of TwoPhaseolus mungoL. Cultivars Induced by Arbuscular Mycorrhizal Fungi andTrichoderma viride

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Navnita Sharma ◽  
Kuldeep Yadav ◽  
Ashok Aggarwal
Keyword(s):  
Mycorrhizal Fungi ◽  
Growth Response ◽  
Trichoderma Viride ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Growth Enhancement ◽  
Acaulospora Laevis ◽  
Am Symbiosis ◽  
The Difference

The present investigation aimed to quantify the difference in response of twoPhaseolus mungoL. cultivars (i.e., UH-1 and IPU-94-1) toGlomus mosseae(G), that is,Funneliformis mosseae,Acaulospora laevis(A), andTrichoderma viride(T), in different combinations or alone. All the treatments were inoculated withBradyrhizobium japonicumto ensure nodulation as soil used in the experiment was sterilized. After 120 days of inoculation, plants were analyzed for chlorophyll content, nodulation, mycorrhization, leaf area, and protein content. Results indicate variation in growth response of two cultivars with different treatments. Triple inoculation of plants with G + A + T proved to be the best treatment for growth followed by G + T in both cultivars. Our work allowed the selection ofP. mungoL. cultivar UH-1 as highly mycorrhizal responsive as compared to IPU-94-1 andG. mosseaeto be an efficient bioinoculant as compared toA. laevisfor growth enhancement ofP. mungo. Further characterization ofP. mungogenotypes will enhance our knowledge of physiological and genetic mechanism behind increase in plant growth and yield due to AM symbiosis.

scholarly journals Pengaruh pemberian takaran fungi mikoriza arbuskular (FMA) terhadap pertumbuhan bibit kopi arabika (Coffea arabica L.)

Jurnal Agro ◽  
10.15575/1813 ◽  
2018 ◽  
Vol 5 (1) ◽  
pp. 61-65
Author(s):  
Lia Sugiarti ◽  
Yana Taryana
Keyword(s):  
Mycorrhizal Fungi ◽  
Coffea Arabica ◽  
Block Design ◽  
Research Result ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Research Station ◽  
Arabica Coffee ◽  
Sea Levels ◽  
Coffea Arabica L

Fungi Mikoriza Arbuskular (FMA) banyak ditemukan pada perakaran kopi. Tujuan dari percobaan ini untuk mempelajari pengaruh pemberian takaran FMA terhadap pertumbuhan bibit kopi Arabika (Coffea arabica L). Percobaan dilaksanakan dari bulan Mei sampai dengan bulan Agustus 2017, bertempat di Kebun Percobaan Fakultas Pertanian Universitas Winaya Mukti, Tanjungsari, Sumedang, dengan ketinggian tempat 850 m dpl. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) terdiri atas enam perlakuan dan diulang sebanyak empat kali.  Perlakuan takaran FMA jenis Glomus agregatum yang terdiri dari A = 0  g tan-1; B = 10 g tan -1; C = 20 g tan-1; D = 30 g tan-1; E = 40 g tan-; dan F = 50 g tan-1. Hasil percobaan menunjukkan bahwa pemberian takaran inokulasi FMA  berpengaruh terhadap tinggi tanaman dan bobot kering tanaman. Pemberian takaran inokulasi FMA dengan takaran 40 g tan-1- sampai 50 g tan-1 memberikan pengaruh yang terbaik terhadap tinggi tanaman dan bobot kering tanaman. Dengan demikian pemberian FMA 40 g tan-1- sampai 50 g tan-1 dapat digunakan pada pembibitan kopi arabika. Arbuscular Mycorrhizal Fungi (AMF) is commonly found in coffee roots.  The purpose of the research was to study effect of AMF application on growth of arabica coffee seedling (Coffea arabica L). The research was conducted from May to August 2017, at Research Station of Agriculture Faculty Universitas Winaya Mukti, Tanjungsari Sumedang, at 850 m above sea levels. The research used Randomized Block Design (RBD) consisted of six treatments and four replications. The treatment was dosage of AMF from Glomus agregatum type i.e. : A = 0 g plant-1; B = 10 g  plant-1; C = 20 g plant-1; D = 30 g plant-1; E = 40 g plant-1; F = 50 g plant-1. Each plot consisted of 10 polybag with 4 sample plants. The research result showed that application of AMF affected on plant height and dry weight of plant, which the best effect was generated by 40 g plant-1- until 50 g plant-1 dosages . It is simply that AMF 40 g plant-1 - 50 g plant-1  can be used in arabica coffee nursery.

scholarly journals Response of Strawberry plant (Fragaria ananassa Duch.) to inoculation with arbuscular mycorrhizal fungi and Trichoderma viride

2010 ◽  
Vol 2 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Sonika Chauhan ◽  
Aditya Kumar ◽  
Chhavi Mangla ◽  
Ashok Aggarwal
Keyword(s):  
Mycorrhizal Fungi ◽  
Trichoderma Viride ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Root Weight ◽  
Maximum Increase ◽  
Positive Role ◽  
Acaulospora Laevis ◽  
Shoot Weight

The present paper represents the positive role of Arbuscular Mycorrhizal (AM) fungi as biofertilizers in strawberry. Experiments were carried out to assess the effectiveness of Trichoderma viride and AM fungi (Glomus mosseae and Acaulospora laevis) alone or in combination, on the growth and biomass production of strawberry. After 120 days, dual inoculation of A. laevis + T. viride showed maximum increase in plant height (30.5±0.3), fresh shoot weight (10.16±0.20), dry shoot weight (2.82±0.02), fresh root weight (6.70±0.10), total chlorophyll (0.841±0.05) and phosphorus content in root (1.13±0.02) as compared to control. However root colonization and AM spore number were maximum in G. mosseae + A. lavies (90.76±1.32) and in G. mosseae (211.16±2.56) respectively as compared to uninoculated plants. Triple inoculation of G. mosseae + A. laevis + T. viride (12.33± 057) was effective in increasing the leaf area.

scholarly journals Interference by Palisade Grass on Coffee Seedlings Inoculated with Arbuscular Mycorrhizal Fungi

2016 ◽  
Vol 34 (4) ◽  
pp. 681-690 ◽  
Author(s):  
A.O. ALECRIM ◽  
A.C. FRANÇA ◽  
E.A. SANTOS ◽  
S.D. MOREIRA ◽  
F.D.S. LEAL ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Randomized Block Design ◽  
P Accumulation ◽  
Zn Content ◽  
Urochloa Brizantha

ABSTRACT The purpose of this paper was to evaluate the effects of the interference by Urochloa brizantha on coffee seedlings inoculated with arbuscular mycorrhizal fungi. A randomized block design and a 4 x 2 layout was used, where factor A was constituted by seedlings inoculated with Rhizophagus clarus, Claroideoglomus etunicatum and Dentiscutata heterogama, as well as some that were not inoculated, and factor B, constituted by plants in competition or alone. The sowing was conducted on washed sand to produce the coffee seedlings, which, during the matchstick stage, were transplanted into polyethylene bags, and inoculated when showing six pairs of leafs. Then, they were transplanted into pots where they were subjected to interference from two plants of U. brizantha for 90 days, during which time their growth and nutrient accumulation were evaluated on the leaves. As a result, an interaction between the following factors was observed: plant height, leaf area, shoot dry weight, dry weight for roots and P, Mg and Zn content in coffee seedlings. The damaging effect on the growth and P accumulation caused by the weed was reduced in coffee seedlings inoculated with mycorrhiza. Another conclusion was that the inoculation factor increases the competitive power of coffee seedlings.

scholarly journals Hongos formadores de micorrizas arbusculares (HFMA) como estrategia para reducir la absorción de cadmio en plantas de cacao (Theobroma cacao)

2019 ◽  
Vol 37 (2) ◽  
pp. 121
Author(s):  
Urley Adrian Pérez Moncada ◽  
María Margarita Ramírez Gómez ◽  
Diana Paola Serralde Ordoñez ◽  
Andrea María Peñaranda Rolón ◽  
Wilmar Alexander Wilches Ortiz ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Theobroma Cacao ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Randomized Complete Block Design ◽  
The World ◽  
Leaves And Roots ◽  
Potential Use

Colombia is among the countries of Latin America that produce about 80% of f ine or “prime” cocoa of the world; however, cocoa in Colombia has limitations related to the presence of cadmium (Cd) in some soils where it is grown and this reduces the safety of the product. The objective of this study was to evaluate the absorption of cadmium in a cocoa pattern (IMC 67) in association with arbuscular mycorrhizal fungi (AMF) to determine their potential use as Cd immobilizers in medium and long-term. A randomized complete block design with three replications and 20 treatments was established, in 4 × 5 factorial arrangement, one control, three types of mycorrhizae (Glomus macrocarpum, Rhizoglomus intraradices and AMF-native) and f ive Cd doses (0, 6, 12, 18 and 24 mg kg-1). Plant height, dry weight of stem, leaves and roots as well as Cd concentration in stems, leaves and roots were measured. The results showed that cacao plants translocate the Cd easily, that is, they can live under these conditions since it was found in all organs (stems, leaves and roots); however, the treatment inoculated with AMF‑native decreased signif icantly the accumulation of Cd in all the organs evaluated. These results conf irm the benef icial effects that AMF have to alleviate the stress that plants shows when translocating heavy metals such as cadmium.

Response of Taxus times media var. densiformis to inoculation with arbuscular mycorrhizal fungi

1998 ◽  
Vol 28 (1) ◽  
pp. 150-153
Author(s):  
J N Gemma ◽  
R E Koske ◽  
E M Roberts ◽  
S Hester
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Root Systems ◽  
Stem Diameter ◽  
Rooted Cuttings ◽  
Shoot Dry Weight ◽  
Mycorrhizal Plants

Rooted cuttings of Taxus times media var. densiformis Rehd. were inoculated with the arbuscular mycorrhizal fungi Gigaspora gigantea (Nicol. & Gerd.) Gerd. & Trappe or Glomus intraradices Schenck and Smith and grown for 9-15 months in a greenhouse. At the completion of the experiments, leaves of inoculated plants contained significantly more chlorophyll (1.3-4.1 times as much) than did noninoculated plants. In addition, mycorrhizal plants had root systems that were significantly larger (1.3-1.4 times) and longer (1.7-2.1 times) than nonmycorrhizal plants, and they possessed significantly more branch roots (1.3-2.9 times). No differences in stem diameter and height or shoot dry weight were evident at the end of the experiments, although the number of buds was significantly greater in the cuttings inoculated with G. intraradices after 15 months.

scholarly journals Application of Humic Acid and Arbuscular Mycorrhizal Fungi to Increase Growth and Yields of Soybean in Ultisol

2020 ◽  
Vol 3 (2) ◽  
pp. 56-64
Author(s):  
Rahayu Arraudah ◽  
Yudhy Harini Bertham ◽  
Hesti Pujiwati ◽  
Bambang Gonggo Murcitro ◽  
Entang Inoriah Sukarjo
Keyword(s):  
Humic Acid ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Agricultural Land ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Two Factors ◽  
Soybean Plants ◽  
Research Stage

Soybean is one of the most popular food crops for the community, but the needs for soybeans have not been fulfilled by soybean production. To meet the needs of soybeans, it is necessary to intensify agricultural land in Ultisol. This study aims to obtain the optimum concentration of humic acid and dosage of the Arbuscular Mycorrhizal Fungi (AMF) to increase soybean plants' production in Ultisols. This research was conducted from January to April 2020 in Beringin Raya Village, Muara Bangkahulu District, Bengkulu City, at an altitude of 10 m above sea level. The research design used a Randomized Complete Block Design (RCBD) two factors with three replications, arranged factorially in experimental units. The first factor is the concentration of humic acid, consisting of 4 levels: 0, 15, 30, and 45 mL L-1 . The second factor is the dose of AMF, consisted of 3 levels, namely: 0, 2.5, and 5 g plant-1. The results showed that the maximum soybean growth and yield in Ultisols were obtained from the humic acid concentration at 45 mL L-1 at the dose of AMF at 2.5 g plant-1 . The resulting production potential is 1.99 tons ha-1 . The administration of humic acid or AMF independently at this research stage had not yet given a maximum response to the growth and yield of soybean in Ultisol.

scholarly journals Evaluasi produksi dan kualitas inokulum fungi mikoriza arbuskula yang diproduksi dengan teknik hidroponik pada rumput Brachiaria decumbens var. mullato

2018 ◽  
Vol 16 (2) ◽  
pp. 10
Author(s):  
A T Aryanto ◽  
P DMH Karti ◽  
I Prihantoro
Keyword(s):  
Host Plant ◽  
Mass Production ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Irrigation System ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
P Uptake ◽  
Brachiaria Decumbens ◽  
Technique System

<p>ABSTRAK<br /><br />Pengembangan hijauan membutuhkan pupuk ramah lingkungan. Fungi Mikoriza Arbuskular (FMA) adalah asosiasi yang melibatkan jamur dan akar yang dianggap sebagai pupuk hayati untuk meningkatkan produktivitas tanaman dan toleran dari kondisi lingkungan. Ketersediaan FMA masih jarang, sehingga dibutuhkan produksi massal untuk dapat mendukung pengembangan hijauan. Tujuan dari penelitian ini adalah untuk menghasilkan inokulum AMF menggunakan sistem hidroponik dalam jumlah besar secara efektif. Penelitian ini dibagi menjadi 2 tahap. Rancangan Acak Kelompok Faktorial yang digunakan pada tahap pertama dengan faktor A adalah jenis sistem irigasi (Manual, Drip dan Nutrien Film Technique System (NFT)) dan B adalah larutan nutrisi (AB Mix dan Hyponex Red) dengan Pueraria javanica sebagai tanaman inang. Rancangan Acak Lengkap (RAL) dilakukan pada tahap kedua dengan menggunakan produksi inokulum FMA dari tahap pertama dengan Brachiaria decumbens var Mullato sebagai tanaman inang. Hasil penelitian menunjukkan bahwa interaksi antara sistem NFT dan AB Mix signifikan (P&lt;0,05) menghasilkan bahan kering tajuk, bahan kering akar dan spora paling tinggi. Semua tipe sistem irigasi dan nutrisi menunjukkan infeksi akar&gt;96%. FMA inokulasi di Brachiaria decumbes var Mulato signifikan(P &lt;0,05) pada bahan kering tajuk, kandungan N, kandungan P dan serapan P.</p><p><br />Kata kunci: Brachiaria decumbes, FMA, sistem Drip, sistem NFT, Pueraria javanica,</p><p><br />ABSTRACT</p><p>Forage mass production development requires environmental friendly fertilizer. Arbuscular Mycorrhizal Fungi (AMF) are mutualitic symbioses between plant and fungi that considered as natural biofertilizer with benefit to improve plant productivitity and environment stress tolerance. The availability of AMF is still low, so it takes mass production to be able to support forage development. The aim of the research was to produce AMF inoculum using hydroponic system in large quantities. This research divided into 2 stages. The factorial randomized block design was used for the first stage with A factor was type of irrigation system (Manual, Drip and Nutrient Film Technique System (NFT)) and B was the nutritional solution (AB Mix and Hyponex Red) using Pueraria javanica as host plant. Completely randomized design was conducted for the second stage by using AMF inoculum production from first stage using Brachiariadecumbens var Mullat as host plant. The best result was a combination beetwen NFT system and AB Mix significantly (P&lt;0.05) produce highest shoot dry matter, root dry matter and spore production. All type of irrigation system and nutrition showed root infection &gt;96%. AMF inoculation in Brachiariadecumbes var Mulato was significant different (P&lt;0.05) on shoot dry matter, N content, P content and P uptake.</p><p><br />Keywords: AMF, B. decumbes, Drip system, NFT system, P. javanica,</p>

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