scholarly journals The Strategy for Marker-Assisted Breeding of Anthocyanin-Rich Spring Bread Wheat (Triticum aestivum L.) Cultivars in Western Siberia

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1603
Author(s):  
Elena Gordeeva ◽  
Vladimir Shamanin ◽  
Olesya Shoeva ◽  
Tatyana Kukoeva ◽  
Alexey Morgounov ◽  
...  

In wheat, anthocyanin pigments can be accumulated in pericarp tissues (under control of the Pp genes) resulting in purple-colored grain. In the current study, a strategy, based on the use of molecular and morphological markers, was applied to create purple-grained bread wheat cultivars adapted to the West Siberian region. The breeding scheme started from crossing of recipients (elite cultivars and lines) with donor lines carrying dominant alleles of the complementary genes Pp3 and Pp-D1. The F2 hybrids passed three-step marker-assisted selection, and those having dominant Pp-D1Pp-D1Pp3Pp3 genotypes were backcrossed with the recurrent parents. The desired BC1F2-3 progenies were selected using morphological marker, while BC1F3 also passed through field evaluation. At this stage, 120 lines were selected and planted in individual 1 m2 “breeding nursery (BN) plots” for assessment of heading dates, duration of vegetation period, resistance to powdery mildew, stem and leaf rusts, protein and gluten content, as well as productivity. After these investigations, a total of 17 promising anthocyanin-rich purple-grained lines characterized by multiple resistance and having best yield/quality characteristics were finally candidates for selection of commercial cultivars adapted to the West Siberian climate and suitable for functional food production.

2020 ◽  
Vol 73 (2) ◽  
pp. 9131-9141
Author(s):  
Zine El Abidine Fellahi ◽  
Abderrahmane Hannachi ◽  
Hamenna Bouzerzour

This study aimed at evaluating the expected gains from selection obtained based upon direct, indirect, and index-based selection in a set of 599 bread wheat lines. The experiment was carried out at the experimental field of INRAA institute, Setif research unit (Algeria), in a Federer augmented block design including three controls. A wide range of genetic variability was observed among lines for the eleven traits assessed. The results indicated that index-based selection and selection based on grain yield expressed higher expected genetic gain than direct and indirect mono-trait-based selection. The best 15 selected lines exhibited higher grain yield than the control varieties, and they were clustered in three groups that contrasted mainly for the flag-leaf area, thousand-kernel weight, biomass, and harvest index. The index-based selection appears as a useful tool for the rapid selection of early filial generations, enriching selected breeding materials with desirable alleles and reducing the number of years required to combine these traits in elite varieties.


2019 ◽  
Vol 55 (No. 4) ◽  
pp. 138-145 ◽  
Author(s):  
Salem Marzougui ◽  
Mohamed Kharrat ◽  
Mongi ben Younes

Identifying QTLs (quantitative trait loci) that control yield related traits under a stressed environment is very useful for marker-assisted selection (MAS). Marker-trait associations (MTA) for several agro-morphological traits were performed with 130 Tunisian and exotic spring bread wheat (Triticum aestivum L.) accessions under a semi-arid climate in El Kef, Tunisia. Grain yield and other important traits were evaluated. A population structural analysis identified two sub populations. In total, 29 MTAs were detected at –log P ≥ 3 using an MLM (mixed linear model), and only 5 MTAs with –log P ≥ 4. The locus on chromosome 4A was detected to control the heading date accounting for up to 22% of the trait variance. Two other loci located on chromosomes 3B and 7B were found to be stable during the two cropping seasons and have a pleiotropic effect on the heading date, yield, internodes length and grain per spike. These two regions are candidates for further genetic analysis.  


1995 ◽  
Vol 46 (3) ◽  
pp. 463 ◽  
Author(s):  
RM Trethowan

This paper examines the success of selection for preharvest sprouting tolerance in white-grained bread wheat using a standard wetting treatment, germination of hand-threshed seed and falling number measurements. The rain simulator was usefull in shifting the population mean of field grown material towards higher levels of tolerance in successive years; however, large genotype x year interactions in material sown under rain protection did not allow accurate assessment of individual genotypes. The most accurate assessments were achieved using falling number measurements (h2 = 80.7%) and hand-threshed seed germinations (h2 = 38.4%), where no genotype x year interactions were recorded. Seed dormancy (determined from hand threshed grain) correlated significantly with change in falling number following 3 days' treatment in the rain simulator ( r = -0-56**). Visual measurements scored in the rain simulator, however, did not correlate significantly with seed dormancy in the first year (r = 0.20) but correlated strongly in the second (r = 0.73***). In comparisons of the same test between years, falling number (without rain treatment) and seed dormancy were significantly correlated (r = 0.68* and 0.90***, respectively), whilst visual scores of sprouting showed no association (r = -0.03).


2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Zine El Abidine Fellahi ◽  
Abderrahmane Hannachi ◽  
Hamenna Bouzerzour

Three selection methods including direct and indirect selection along with selection index based on the phenotypic values of eleven traits of agronomic interest were assessed for their application in F4 bread wheat progenies. Significant genetic variation existed among parents and crosses for the traits measured. The following were the most efficient indices for simultaneous selection of superior lines for yield and its components: base index of Williams, followed by the sum of ranks index of Smith and Hazel. The selection-based index provided the highest grain yield gains as compared to the other selection criteria, except for flag leaf area, indicating that the direct and indirect monotrait selection were not appropriate in the situation analyzed in this work. PCA identified Ain Abid × Mahon-Demias, Ain Abid × Rmada, and Ain Abid × El-Wifak as the most promising populations. At 5% selection intensity, the top 30 lines selected were distinguished, in comparison with the standard check Hidhab, by significant improvements in yield and yield components.


2021 ◽  
Vol 182 (2) ◽  
pp. 81-88
Author(s):  
B. V. Rigin ◽  
Е. V. Zuev ◽  
А. S. Andreeva ◽  
I. I. Matvienko ◽  
Z. S. Pyzhenkova

Background. Development of early-ripening spring bread wheat (Triticum aestivum L.) cultivars is an important task for Russian breeders. Knowledge of the genetics of ultraearly varieties – sources of valuable genes that determine an earlier-maturing type of plant development –will be used to work out methods for obtaining source material for breeding.Materials and methods. The ultra-early lines Rico (k65588, var. erythrospermum Koern.) and Rimax (k-67257, var. lutescens (Alef.) Mansf.), and cvs. ‘Max’ (k-57181, var. lutescens) and ‘Leningradskaya 6’ (k-64900, var. lutescens) were studied. Alleles of the Vrn and Ppd genes were identified by PCR according to known publications, and genomic DNA was isolated from 3-day-old seedlings by the CTAB method.Results. In the environments of Northwest Russia, the Rimax and Rico lines are characterized by the highest rate of development before heading among the spring wheat accessions from the VIR collection. In the Rimax and Rico genotypes, the Vrn-A1, Vrn-B1, Vrn-D1 and Ppd-D1 genes were found. Genotypes with different alleles of Ppd-D1 and Vrn-B1 were identified in the Rimax line. Under conditions of a long day (18 hours), in the population of F2  (F3 ) Rico × Rimax hybrids, the ratio of phenotypes with a high development rate to all others was observed as 1 : 15 (χ2 1: 15 = 0.64). Under a short day (12 hours), 5 clearly tested groups were identified in F2 with the ratio 1 : 4 : 6 : 4 : 1 (χ2  = 3.03; χ2 0.05 = 9.48), which indicates the manifestation of cumulative polymerization.Conclusion. Each of the Rimax and Rico lines has two pairs of independent duplicated genes that determine a high development rate. Under short-day conditions, these genes can interact like cumulative polymers. The Rimax and Rico lines, due to their high development rate, are valuable source material to be used in breeding for earliness.


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