scholarly journals Evaluation of Resistance Sources of Tomato (Solanum lycopersicum L.) to Phylotype I Strains of Ralstonia solanacearum Species Complex in Benin

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1513
Author(s):  
Herbaud Zohoungbogbo ◽  
Adonis Quenum ◽  
Judith Honfoga ◽  
Jaw-Rong Chen ◽  
Enoch Achigan-Dako ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Block Design ◽  
Agricultural System ◽  
Sources Of Resistance ◽  
Breeding Programs ◽  
Solanum Lycopersicum L ◽  
The University ◽  
Phylotype I

Finding sources of resistance to bacterial wilt (BW) caused by Ralstonia solanacearum species complex is a crucial step toward the development of improved bacterial wilt-resistant tomato varieties. Here, we evaluated new sources of bacterial wilt-tolerant/resistant tomato lines and identified associated phylotype/sequevar of R. solanacearum strains in Benin. Eighteen F5 lines and five checks were evaluated in two hotspots: the experimental site of the World Vegetable Center, Cotonou Benin, and the Laboratory of Genetics, Biotechnology and Seed Science of the University of Abomey-Calavi. Experiments were laid out in a randomized complete block design with four replicates. Data were collected on bacterial wilt incidence, horticultural and fruit traits and yield components. Across the two experiments, the F5 lines showed no wilting, while the local variety ‘Tounvi’ used as susceptible check showed 57.64% wilting. The wilting was due to BW and was associated with sequevars I-14, I-18 and I-31 of phylotype I. AVTO1803, AVTO1955-6 and H7996 were the highest yielding lines with 20.29 t·ha−1, 17.66 t·ha−1 and 17.07 t/ha, respectively. The sources of resistance to BW can be recommended to national agricultural system for dissemination or used in tomato breeding programs.

scholarly journals Screening Cultivated Eggplant and Wild Relatives for Resistance to Bacterial Wilt (Ralstonia solanacearum)

Agriculture ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 157 ◽  
Author(s):  
Namisy ◽  
Chen ◽  
Prohens ◽  
Metwally ◽  
Elmahrouk ◽  
...  
Keyword(s):  
High Temperatures ◽  
Resistance Genes ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Solanum Melongena ◽  
Wild Relatives ◽  
Sources Of Resistance ◽  
Race 1 ◽  
Moderately Resistant ◽  
Phylotype I

Bacterial wilt, caused by Ralstonia solanacearum, is highly diverse and the identification of new sources of resistance for the incorporation of multiple and complementary resistance genes in the same cultivar is the best strategy for durable and stable resistance. The objective of this study was to screen seven accessions of cultivated eggplant (Solanum melongena L.) and 40 accessions from 12 wild relatives for resistance to two virulent R. solanacearum strains (Pss97 and Pss2016; phylotype I, race 1, biovar 3). The resistant or moderately resistant accessions were further evaluated with Pss97 in a second trial under high temperatures (and also with Pss2016 for S. anguivi accession VI050346). The resistant control EG203 was resistant to Pss97, but only moderately resistant to Pss2016. One accession of S. sisymbriifolium (SIS1) and two accessions of S. torvum (TOR2 and TOR3) were resistant or moderately resistant to Pss97 in both trials. Solanum anguivi VI050346, S. incanum accession MM577, and S. sisymbriifolium (SIS1 and SIS2) were resistant to Pss2016 in the first trial. However, S. anguivi VI050346 was susceptible in the second trial. These results are important for breeding resistant rootstocks and cultivars that can be used to manage this endemic disease.

scholarly journals Molecular Epidemiology of Ralstonia solanacearum Species Complex Strains Causing Bacterial Wilt of Potato in Uganda

2019 ◽  
Vol 109 (11) ◽  
pp. 1922-1931 ◽  
Author(s):  
Abdulwahab Abdurahman ◽  
Monica L. Parker ◽  
Jan Kreuze ◽  
John G. Elphinstone ◽  
Paul C. Struik ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Nationwide Survey ◽  
Potato Production ◽  
Repeat Sequence ◽  
Latently Infected ◽  
Pure Cultures ◽  
Diversity Studies ◽  
Phylotype I

Bacterial wilt (BW) caused by the Ralstonia solanacearum species complex (RSSC) is a serious threat to potato production in Uganda. However, little is known about the extent of the disease and the type of the pathogen strains involved. A nationwide survey was conducted to study BW prevalence and incidence in potato, and potato tuber and stem samples of potential alternative hosts were collected for pathogen isolation. DNA was extracted from pure cultures for genetic diversity studies. The pathogen was phylotyped by multiplex PCR; then, a subset of isolates was typed at sequevar level. Isolates of the same sequevar were then haplotyped using multilocus tandem repeat sequence typing (TRST) schemes. BW prevalence and incidence in potato farms were 81.4 and 1.7%, respectively. Three RSSC phylotypes were identified, with the majority of the strains belonging to Phylotype II (80%) followed by Phylotype I (18.5%) and III (1.5%). Phylotype I strains belonged to Sequevar 31, and Phylotype II strains belonged to Sequevar 1. Potato-associated Phylotype II Sequevar 1 strains were more diverse (27 TRST haplotypes) than nonpotato Phylotype I (5 TRST haplotypes). Mapping of TRST haplotypes revealed that three TRST haplotypes of Phylotype II Sequevar 1 strains play an important epidemiological role in BW of potato in Uganda being disseminated via latently infected seed. [Formula: see text] Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Distribution and incidence of tomato bacterial wilt caused by Ralstonia solanacearum in the central region of Togo

2021 ◽  
Author(s):  
Bitang Bamazi ◽  
Agnassim Banito ◽  
K. D. Ayisah ◽  
Rachidatou Sikirou ◽  
Mathews Paret ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Laboratory Investigation ◽  
Central Region ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Yield Losses ◽  
Tomato Production ◽  
Solanum Lycopersicum L ◽  
Survey Results ◽  
Tomato Bacterial Wilt

Tomato (Solanum lycopersicum L.) is one of the most important vegetables in Togo. Unfortunately, tomatoes are susceptible to many diseases, among which bacterial wilt caused by Ralstonia solanacearum causes major yield losses. In this study, incidence of bacterial wilt and its distribution was evaluated in the central region of Togo, the major tomato producing area in the country. Overall, 16 localities were surveyed in four prefectures. In each locality, three fields were visited, and the incidence of the disease was recorded, and diseased samples were collected for laboratory investigation. The results showed that bacterial wilt occurred in all the fields visited, indicating a field incidence of 100%, whereas the plant incidence ranged from 10.00±00% to 43.33±3.33%, with an average of 20.94±1.77%. The antibody based Immunostrip test was positive for R. solanacearum in 100% of the visited fields. From 144 samples collected from fields, 45 R. solanacearum isolates were isolated on Modified SMSA media. This survey results show that tomato bacterial wilt is a real threat to tomato production in the central region of Togo.

scholarly journals First Report of Ralstonia solanacearum Phylotype I Causing Bacterial Wilt on Rosa spp. in Korea

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1407
Author(s):  
Y. S. Kim ◽  
S. R. Lim ◽  
J.-W. Kim ◽  
H.-J. Lee ◽  
D. H. Park
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
First Report ◽  
Phylotype I

scholarly journals First Report of Bacterial Wilt of Tomato Caused by Ralstonia pseudosolanacearum (Ralstonia solanacearum phylotype I) in Cambodia

Plant Disease ◽  
2020 ◽  
Vol 104 (3) ◽  
pp. 969-969
Author(s):  
T. L. Klass ◽  
M. M. Hayes ◽  
K. H. Seng ◽  
C. An ◽  
F. Rotondo ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
First Report ◽  
Ralstonia Pseudosolanacearum ◽  
Phylotype I

scholarly journals Reaction of Capsicum peppers commercialized in the Federal District to bacterial wilt

2018 ◽  
Vol 36 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Maurício Rossato ◽  
Thais R. Santiago ◽  
Carlos Alberto Lopes
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Bacterial Pathogens ◽  
Federal District ◽  
Capsicum Frutescens ◽  
Capsicum Baccatum ◽  
Main Species ◽  
Race 1 ◽  
Resistant Group ◽  
Phylotype I

ABSTRACT In Brazil, the bacterial pathogens Ralstonia solanacearum and R. pseudosolanacearum cause substantial losses by inducing bacterial wilt on several solanaceous crops; R. pseudosolanacearum is the main species associated with peppers (Capsicum sp.). To verify the bacterial wilt reaction on Capsicum peppers commercialized in the Federal District (DF), fruits of several genotypes within this genus were collected from six different fairs distributed in the satellite cities of Gama, Sobradinho and Guará. Seedlings with four true leaves derived from seeds extracted from such fruits were root inoculated with 108 CFU/mL with a representative isolate of R. pseudosolanacearum (race 1, biovar 3, phylotype I, sequevar 18). The evaluated species were: Capsicum frutescens (‘pimenta-malagueta’), Capsicum baccatum var. pendulum (‘pimenta-dedo-de-moça’) and C. chinense (‘pimenta-de-bode’ red and yellow, ‘pimenta-cumarí-do-Pará’, ‘pimenta-biquinho’, ‘pimenta-habanero’ and ‘pimenta-de-cheiro’). Not all species were found in all six fairs. The reaction to bacterial wilt was variable and species-dependent. From 26 evaluated genotypes, none presented an immune-like response, 10 were considered resistant and 16 susceptible based on wilt incidence (Scott-Knott, 5%). Four Capsicum baccatum accesses were positioned in the resistant group, whereas 14 out of 18 of C. chinense were susceptible. Capsicum frutescens showed variable reactions. These results contribute to indicate cultivation of specific groups of pepper according to the presence of the pathogen in the soil.

scholarly journals Bacterial Wilt Resistance in Tomato, Pepper, and Eggplant: Genetic Resources Respond to Diverse Strains in the Ralstonia solanacearum Species Complex

2011 ◽  
Vol 101 (1) ◽  
pp. 154-165 ◽  
Author(s):  
A. Lebeau ◽  
M.-C. Daunay ◽  
A. Frary ◽  
A. Palloix ◽  
J.-F. Wang ◽  
...  
Keyword(s):  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Effective Control ◽  
Economic Losses ◽  
Wilt Resistance ◽  
The Core ◽  
Intermediate Phenotypes ◽  
Vascular Elements ◽  
Pathogen Strains

Bacterial wilt, caused by strains belonging to the Ralstonia solanacearum species complex, inflicts severe economic losses in many crops worldwide. Host resistance remains the most effective control strategy against this disease. However, wilt resistance is often overcome due to the considerable variation among pathogen strains. To help breeders circumvent this problem, we assembled a worldwide collection of 30 accessions of tomato, eggplant and pepper (Core-TEP), most of which are commonly used as sources of resistance to R. solanacearum or for mapping quantitative trait loci. The Core-TEP lines were challenged with a core collection of 12 pathogen strains (Core-Rs2) representing the phylogenetic diversity of R. solanacearum. We observed six interaction phenotypes, from highly susceptible to highly resistant. Intermediate phenotypes resulted from the plants' ability to tolerate latent infections (i.e., bacterial colonization of vascular elements with limited or no wilting). The Core-Rs2 strains partitioned into three pathotypes on pepper accessions, five on tomato, and six on eggplant. A “pathoprofile” concept was developed to characterize the strain clusters, which displayed six virulence patterns on the whole set of Core-TEP host accessions. Neither pathotypes nor pathoprofiles were phylotype specific. Pathoprofiles with high aggressiveness were mainly found in strains from phylotypes I, IIB, and III. One pathoprofile included a strain that overcame almost all resistance sources.

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