scholarly journals Bacillus subtilis CBR05 for Tomato (Solanum lycopersicum) Fruits in South Korea as a Novel Plant Probiotic Bacterium (PPB): Implications from Total Phenolics, Flavonoids, and Carotenoids Content for Fruit Quality

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 838 ◽  
Author(s):  
Murugesan Chandrasekaran ◽  
Se Chul Chun ◽  
Jae Wook Oh ◽  
Manivannan Paramasivan ◽  
Ramesh Kumar Saini ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antioxidant Activities ◽  
Plant Growth Promoting Rhizobacteria ◽  
Probiotic Bacterium ◽  
Tomato Fruits ◽  
Tomato Production ◽  
Market Sample ◽  
Plant Growth Promoting ◽  
Pgpr Strain ◽  
Β Carotene

Plant growth-promoting rhizobacteria (PGPR) are naturally occurring soil biota which benefit plants by improving plant productivity and immunity. The aim of the present work was to evaluate the effect of the inoculation of PGPR strain, Bacillus subtilis CBR05 on the quality of tomato fruits produced under greenhouse conditions. Results were compared with mock-inoculated control and market sample. We found a significant increase in total phenol and flavonoid contents of tomato fruits in PGPR strain B. subtilis CBR05 inoculated plants compared to those of mock-inoculated control and market sample. Moreover, B. subtilis CBR05 inoculation stimulated antioxidant activities and levels of carotenoid (β carotene and lycopene) content in plants. In addition, the inoculation of the strain B. subtilis CBR05 produced the highest content of lycopene (21.08 μg/g FW) in tomato fruits as compared to mock-inoculated plants. Our results show that the PGPR strain B. subtilis CBR05 is a versatile soil bacterium that enhances tomato production by elevating antioxidant activities and carotenoid (β carotene and lycopene) levels in fruit.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals Influence of Plant growth promoting Rhizobacteria on growth and Biochemical Parameters of Corn (Zea mays l.) Var. Seedlings

2020 ◽  
Vol 1 (3) ◽  
pp. 9-24
Author(s):  
Huma Nawaz ◽  
Saima Javed ◽  
Muhammad Faisal
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Biochemical Parameters ◽  
Agricultural Practices ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Maximum Increase ◽  
Pot Trials ◽  
Plant Growth Promoting ◽  
Bacillus Strains

Impact of inoculations of eight individual strains (CN2, CSH4, Cu35, CMS7, CSH23, CSH27, CF18, and Cu47, two combinations (Comb 4A and Comb 4B) of four Bacillus strains and mixture of all strains (Mix) was studied. Various growth and biochemical parameters of maize plant were determined in pot trials. Strain Bacillus subtilis CN2 showed 49% maximum increase in shoot length. The blend of entire strains was observed to have their lenghth increased about 21 %. Bacillus subtilis CN2 showed maximum increase 155%, in fresh biomass and 233 % increase in dry weight. Bacillus amyloliquefaciens (Cu47) and Comb 4A and showed maximum peroxidase content of 163 % and 94 % as compared to uninoculated control. Comb 4B was shown to have significant higher content of acidphosphatase (811 %). Increase in all other physical and biochemical parameters were noticed. Therefore, Bacillus strains exhibited characteristic increased potential of plant growth and can have great application in innovative agricultural practices.

scholarly journals Pengaruh PGPR terhadap Penekanan Populasi Nematoda Puru Akar (Meloidogyne incognita (Kofoid and White) Chitwood) pada Tanaman Kenaf (Hibiscus cannabinus L.)

2016 ◽  
Vol 8 (1) ◽  
pp. 30
Author(s):  
Kristiana Sri Wijayanti ◽  
Bambang Tri Rahardjo ◽  
Toto Himawan
Keyword(s):  
Bacillus Subtilis ◽  
Meloidogyne Incognita ◽  
Block Design ◽  
Plant Growth Promoting Rhizobacteria ◽  
Hibiscus Cannabinus ◽  
Control Treatment ◽  
Randomized Block Design ◽  
Two Factors ◽  
Plant Growth Promoting ◽  
Nematode Eggs

<p>Tanaman kenaf yang terinfeksi nematoda <em>Meloidogyne incognita</em><em> </em><em> </em>dapat mempengaruhi pertumbuhan tanaman dan produksi serat. Penelitian ini bertujuan untuk mengevaluasi pengaruh <em>Plant Growth Promoting Rhizobacteria </em>(PGPR) dalam menekan populasi nematoda <em>M. incognita</em> pada tanaman kenaf di rumah kaca. Penelitian dirancang dengan menggunakan rancangan acak kelompok (RAK) faktorial dengan dua faktor, faktor pertama adalah cara aplikasi PGPR yang terdiri atas 2 cara yaitu suspensi PGPR diberikan sebelum tanam dengan merendam benih selama 5 jam (C1), benih ditanam langsung dalam pot tanpa direndam dalam PGPR (C2), dan suspensi PGPR diberikan pada 15 hari setelah tanam (HST) dan 25 HST. Faktor kedua adalah jenis PGPR yang digunakan yaitu <em>P</em><em>seudomonas</em><em> fluorescens</em>, <em>Bacillus subtilis, Azotobacter</em> sp., <em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>B. subtilis, </em><em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>Azotobacter</em> sp., <em>B. subtilis </em>+ <em>Azotobacter</em> sp., dan <em>P</em><em>. </em><em>fluorescen</em><em>s </em>+ <em>B. subtilis </em>+ <em>Azotobacter</em> sp., serta kontrol (tanpa PGPR).  Hasil penelitian menunjukkan bahwa perendaman benih dengan kombinasi tiga bakteri memberikan pengaruh yang nyata terhadap populasi juvenil nematoda dalam tanah, sedangkan perlakuan tanpa perendaman tidak memberikan pengaruh. Populasi juvenil nematoda di dalam akar yang diberi PGPR baik tunggal maupun kombinasi melalui perendaman benih atau tanpa perendaman benih tidak berpengaruh, kecuali pada kombinasi <em>P</em><em>.</em><em> fluorescens </em>dan <em>B. subtilis</em><em> </em>yang diberikan melalui perendaman benih mampu menekan populasi juvenil nematoda di akar 43,28% bila dibandingkan tanpa perendaman benih. Pemberian rizobakteri <em>P</em><em>.</em><em> fluorescens</em>  menurunkan jumlah telur nematoda terbanyak (86,39%) dan menekan intensitas penyakit sebesar 71,95% bila dibandingkan kontrol.</p><p><strong></strong>Infection of <em>Meloidogyne incognita</em> on kenaf could affect its growth and the production of fiber. This study aimed to evaluate the effect of PGPR on the reduction of nematode <em>M. incognita</em> population on kenaf in the greenhouse. The factorial experiment was laid on randomized block design. The study consisted of two factors with three replicates . The first factor was method of PGPR application, ie: PGPR suspension was given before planting (kenaf seeds was soaked for 5 hours) (C1) and the seeds directly planted without submerged (C2), PGPR suspension was given at 15 days after planting (DAP) and 25 dap. The second factor was type of bacteria (<em>Pseudomonas  fluorescens</em>,  <em>Bacillus  subtilis,  Azotobacter</em>  sp.,  <em>P.  fluorescens </em>+ <em>B. subtilis, </em><em>P. fluorescens </em>+ <em>Azotobacter</em>  sp., <em>B. subtilis </em>+ <em>Azotobacter</em>  sp.,  and  <em>P.  fluorescens  </em>+  <em>B. subtilis   </em>+ <em>Azotobacter</em>  sp.) and control. The results showed that submerged seed with the three bacterial rhizobacteria significant compared to the control treatment and single treatment and two combination rhizobacteria, while without submerged seed with single or combination rhizobacteria not significant on the population of juvenile nematodes in the soil. Combination of <em>P. fluorescens </em>and<em> B. subtilis</em>with submerged seed capable of suppressing the population of  juvenile  nematodes  in the  roots  of 43.28%  when  compared with or without submerged seed.   Population  of  juvenile  nematodes  in the  roots by submerged seed and without submerged seed either single or combination rhizobacteria do not affect each other. <em>P. fluorescens </em>suppress nematode eggs are highest 86.39% and disease intensity by 71,95% where compared to control.</p>

scholarly journals Quantitative and qualitative basement of microbial presence during phytoremediation of heavy metal polluted soil using Chromolaena odorata

2020 ◽  
Vol 31 (2) ◽  
pp. 145-151
Author(s):  
Gloria Omorowa Omoregie ◽  
Abraham Goodness Ogofure ◽  
Beckley Ikhajiagbe ◽  
Geoffrey Obinna Anoliefo
Keyword(s):  
Heavy Metal ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Plant Growth Promoting Rhizobacteria ◽  
Stem Cuttings ◽  
Chromolaena Odorata ◽  
Plant Growth Promoting ◽  
Bacteria And Fungi ◽  
Disturbed Soils

Abstract The presence and impact of bulk and rhizosphere microorganisms in contaminated soils can be huge, given that they have the ability to increase plants tolerance against abiotic stress, and also enhance plant growth, while supporting hastened remediation of disturbed soils. The present study quantitatively and qualitatively assessed presence of cultural fungi and bacteria during phytoremediation of heavy metal polluted soils using Chromolaena odorata. Stem cuttings of C. odorata were planted in soils polluted with Pb, Mn, Zn, Cd, and Cu at once (1ESC), thrice (3ESC) and five (5ESC) times their respective ecological screening concentrations (ESC). ESC of Pb, Mn and Zn is 50 mg/kg, Cd is 4 mg/kg, and Cu is 100 mg/kg. After 6 months, results showed that more than 10 species of bacteria and fungi were identified in the study, with P. aeruginosa and Bacillus subtilis being the most occurring bacteria while, Penicillium sp. and Aspergillus niger the most occurring fungi in both bulk and rhizospheric soils. The presence of known plant growth promoting rhizobacteria in plants rhizosphere including Azotobacter sp., Bacillus subtilis, B. pumilus, Clostridium sp., P. aeruginosa, and Klebsiella sp. was also reported.

scholarly journals Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

2019 ◽  
Author(s):  
Dongsheng Guo ◽  
Chenhong Yuan ◽  
Yunyan Luo ◽  
YaHan Chen ◽  
Meihuan Lu ◽  
...  
Keyword(s):  
Crude Extract ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Sem Analysis ◽  
Phytophthora Nicotianae ◽  
Black Shank ◽  
Biocontrol Efficacy ◽  
Plant Growth Promoting ◽  
Pgpr Strain

AbstractTobacco black shank (TBS) caused by Phytophthora nicotianae is destructive to almost all kinds of tobacco cultivars and is widespread in many tobacco-planted countries. Here, an isolated plant growth-promoting rhizobacteria (PGPR) strain Ba168 is promise in biocontrol of TBS. In vitro assays disclosed a strong P. nicotianae suppression activity and the field utilization potential (FUP) by characterized the crude extract of culture filtrates of Ba168. P. nicotianae’s growth was inhibited by the crude extract at minimum inhibitory concentration (MIC) of 5μl/mL. Extracellular conductivity, pH and the wet, dry weight of P. nicotianae’s mycelia, were significantly different after treated with different concentrations of the crude extract and the deformity and perforation of treated P. nicotianae’s hyphae can be observed in scanning electron microscope (SEM) analysis. Proteome characterizations of the crude extract were used as supplementary proofs that further evaluated FUP of Ba168. We then identified strain Ba168 as B. amyloliquefaciens by its genetic and phenotypic characteristics. Field assays comparatively evaluated TBS control efficacy of these PGPRs and agrochemicals. Pooling analysis of the results showed that the biocontrol efficacy of Ba168 preparation is only lower than Mixture of Propamocarb hydrochloride and Azoxystrobin (MPA) but better than other tested subjects. Although the existence of differences in biocontrol efficacy, PGPR preparations effectively reduced the disease index of tobacco.ImportanceThis work demonstrates the promising biocontrol potential of B. amyloliquefaciens Ba168 and highlights the positive roles of PGPR in suppression of this soil-borne disease.

scholarly journals Plant Growth-Promoting Rhizobacteria Modulate the Concentration of Bioactive Compounds in Tomato Fruits

Separations ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 223
Author(s):  
Clara de la Osa ◽  
Miguel Ángel Rodríguez-Carvajal ◽  
Jacinto Gandullo ◽  
Clara Aranda ◽  
Manuel Megías ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Plant Growth ◽  
Agricultural Soils ◽  
Antioxidant Activities ◽  
Plant Growth Promoting Rhizobacteria ◽  
Rice Paddy ◽  
Bioactive Molecules ◽  
Cultivated Rice ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: The application of microorganisms as bioestimulants in order to increase the yield and/or quality of agricultural products is becoming a widely used practice in many countries. In this work, five plant growth-promoting rhizobacteria (PGPR), isolated from cultivated rice paddy soils, were selected for their plant growth-promoting capacities (e.g., auxin synthesis, chitinase activity, phosphate solubilisation and siderophores production). Two different tomato cultivars were inoculated, Tres Cantos and cherry. Plants were grown under greenhouse conditions and different phenotypic characteristics were analysed at the time of harvesting. Results: Tres Cantos plants inoculated with PGPR produced less biomass but larger fruits. However, the photosynthetic rate was barely affected. Several antioxidant activities were upregulated in these plants, and no oxidative damage in terms of lipid peroxidation was observed. Finally, ripe fruits accumulated less sugar but, interestingly, more lycopene. By contrast, inoculation of cherry plants with PGPR had no effect on biomass, although photosynthesis was slightly affected, and the productivity was similar to the control plants. In addition, antioxidant activities were downregulated and a higher lipid peroxidation was detected. However, neither sugar nor lycopene accumulation was altered. Conclusion: These results support the use of microorganisms isolated from agricultural soils as interesting tools to manipulate the level of important bioactive molecules in plants. However, this effect seems to be very specific, even at the variety level, and deeper analyses are necessary to assess their use for specific applications.

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