scholarly journals Neoplasia-Associated Wasting Diseases with Economic Relevance in the Sheep Industry

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 381
Author(s):  
Marcelo De las Heras ◽  
Marta Borobia ◽  
Aurora Ortín
Keyword(s):  
New Zealand ◽  
Economic Losses ◽  
Low Grade ◽  
Environmental Carcinogens ◽  
Signet Ring ◽  
The World ◽  
Production Cycles ◽  
Nasal Adenocarcinoma ◽  
In Vivo Diagnosis

We review three neoplastic wasting diseases affecting sheep generally recorded under common production cycles and with epidemiological and economic relevance in sheep-rearing countries: small intestinal adenocarcinoma (SIA), ovine pulmonary adenocarcinoma (OPA) and enzootic nasal adenocarcinoma (ENA). SIA is prevalent in Australia and New Zealand but present elsewhere in the world. This neoplasia is a tubular or signet-ring adenocarcinoma mainly located in the middle or distal term of the small intestine. Predisposing factors and aetiology are not known, but genetic factors or environmental carcinogens may be involved. OPA is a contagious lung cancer caused by jaagsiekte sheep retrovirus (JSRV) and has been reported in most sheep-rearing countries, resulting in significant economic losses. The disease is clinically characterized by a chronic respiratory process as a consequence of the development of lung adenocarcinoma. Diagnosis is based on the detection of JSRV in the tumour lesion by immunohistochemistry and PCR. In vivo diagnosis may be difficult, mainly in preclinical cases. ENA is a neoplasia of glands of the nasal mucosa and is associated with enzootic nasal tumour virus 1 (ENTV-1), which is similar to JSRV. ENA enzootically occurs in many countries of the world with the exception of Australia and New Zealand. The pathology associated with this neoplasia corresponds with a space occupying lesion histologically characterized as a low-grade adenocarcinoma. The combination of PCR and immunohistochemistry for diagnosis is advised.

scholarly journals Nasal swab real-time PCR is not suitable for in vivo diagnosis of bovine tuberculosis

2017 ◽  
Vol 37 (6) ◽  
pp. 549-554
Author(s):  
Fabiana Q. Mayer ◽  
Emily M. dos Reis ◽  
André Vinícius A. Bezerra ◽  
Rogério O. Rodrigues ◽  
Thais Michel ◽  
...  
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Real Time Pcr ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Disease Diagnosis ◽  
Economic Losses ◽  
Nasal Swabs ◽  
In Vivo Diagnosis

ABSTRACT: Bovine tuberculosis (bTB) is a zoonosis causing economic losses and public health risks in many countries. The disease diagnosis in live animals is performed by intradermal tuberculin test, which is based on delayed hypersensitivity reactions. As tuberculosis has complex immune response, this test has limitations in sensitivity and specificity. This study sought to test an alternative approach for in vivo diagnosis of bovine tuberculosis, based on real-time polymerase chain reaction (PCR). DNA samples, extracted from nasal swabs of live cows, were used for SYBR® Green real-time PCR, which is able to differentiate between Mycobacterium tuberculosis and Mycobacterium avium complexes. Statistical analysis was performed to compare the results of tuberculin test, the in vivo gold standard bTB diagnosis method, with real-time PCR, thereby determining the specificity and sensitivity of molecular method. Cervical comparative test (CCT) was performed in 238 animals, of which 193 had suitable DNA from nasal swabs for molecular analysis, as indicated by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and were included in the study. In total, 25 (10.5%) of the animals were CCT reactive, of which none was positive in the molecular test. Of the 168 CCT negative animals, four were positive for M. tuberculosis complex at real time PCR from nasal swabs. The comparison of these results generated values of sensitivity and specificity of 0% and 97.6%, respectively; moreover, low coefficients of agreement and correlation (-0.029 and -0.049, respectively) between the results obtained with both tests were also observed. This study showed that real-time PCR from nasal swabs is not suitable for in vivo diagnosis of bovine tuberculosis; thus tuberculin skin test is still the best option for this purpose.

209 RISKS OF TRANSMITTING MYCOBACTERIUM AVIUM SSP. PARATUBERCULOSIS BY TRANSFER OF IN VIVO-DERIVED AND IN VITRO-FERTILIZED BOVINE EMBRYOS

2006 ◽  
Vol 18 (2) ◽  
pp. 212
Author(s):  
A. Bielanski ◽  
J. Algire ◽  
G. Randall ◽  
O. Surujballi
Keyword(s):  
Mycobacterium Avium ◽  
Disease Transmission ◽  
Economic Losses ◽  
Persistent Diarrhea ◽  
Bovine Embryos ◽  
Causative Microorganism ◽  
The World ◽  
In Vitro Exposure

Paratuberculosis (Johne's disease) is a chronic infectious disease of cattle and other domestic and wild ruminants caused by Mycobacterium avium ssp. paratuberculosis (Map) which is widespread throughout the world. The disease is characterized by persistent diarrhea, weight loss, and eventually death. In addition to interest in the economic losses, there has been an increasing interest in eradicating the disease due to the potential involvement of the causative microorganism in Crohn's disease, a debilitating chronic enteritis in man. Experiments were conducted to determine the possibility of transmission of Map by embryo transfer (ET) and the association of Map with in vivo-derived and in vitro-fertilized (IVF) embryos. These experiments involved (1) collection of embryos from naturally infected donors (n = 5) and transfer of those embryos to uninfected recipients (n = 12) and testing of others for Map by culture on Herrold's medium with mycobactin or by PCR; and (2) in vitro exposure of in vivo-derived and IVF embryos to Map and their subsequent transfer to uninfected recipients. Experiment 1 revealed the presence of Map in the uterine horns of all five subclinically infected donors, but it was not detected in association with sequentially washed in vivo-derived embryos by culture or by PCR. In Experiment 2, a high proportion of both in vivo-derived and IVF embryos exposed to Map in vitro tested positive for Map even after sequential washings as recommended by IETS. Transfer of in vivo-derived and IVF embryos that had been exposed to Map, and then washed, into 18 recipients resulted in 13 pregnancies and 8 calves born without evidence of disease transmission to either the recipients or the offspring over the following 5 year period. In conclusion, it is unlikely that Map will be transmitted by ET when the embryos have been processed according to the washing protocols recommended by the IETS.

Comparison of Immunological and Functional Assays for Measurement of Soluble Fibrin

1995 ◽  
Vol 74 (02) ◽  
pp. 673-679 ◽  
Author(s):  
C E Dempfle ◽  
S A Pfitzner ◽  
M Dollman ◽  
K Huck ◽  
G Stehle ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Low Grade ◽  
Plasma Samples ◽  
Normal Range ◽  
Soluble Fibrin ◽  
Discriminating Power ◽  
Fibrin Monomer ◽  
Cerebral Ischemic

SummaryVarious assays have been developed for quantitation of soluble fibrin or fibrin monomer in clinical plasma samples, since this parameter directly reflects in vivo thrombin action on fibrinogen. Using plasma samples from healthy blood donors, patients with cerebral ischemic insult, patients with septicemia, and patients with venous thrombosis, we compared two immunologic tests using monoclonal antibodies against fibrin-specific neo-epitopes, and two functional tests based on the cofactor activity of soluble fibrin complexes in tPA-induced plasminogen activation. Test A (Enzymun®-Test FM) showed the best discriminating power among normal range and pathological samples. Test B (Fibrinostika® soluble fibrin) clearly separated normal range from pathological samples, but failed to discriminate among samples from patients with low grade coagulation activation in septicemia, and massive activation in venous thrombosis. Functional test C (Fibrin monomer test Behring) displayed good discriminating power between normal and pathological range samples, and correlated with test A (r = 0.61), whereas assay D (Coa-Set® Fibrin monomer) showed little discriminating power at values below 10 μg/ml and little correlation with other assays. Standardization of assays will require further characterization of analytes detected.

Vibrio harveyi virulence gene expression in vitro and in vivo during infection in black tiger shrimp Penaeus monodon

2020 ◽  
Vol 139 ◽  
pp. 153-160
Author(s):  
S Peeralil ◽  
TC Joseph ◽  
V Murugadas ◽  
PG Akhilnath ◽  
VN Sreejith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Virulence Genes ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Virulence Gene ◽  
Economic Losses ◽  
Virulence Gene Expression

Luminescent Vibrio harveyi is common in sea and estuarine waters. It produces several virulence factors and negatively affects larval penaeid shrimp in hatcheries, resulting in severe economic losses to shrimp aquaculture. Although V. harveyi is an important pathogen of shrimp, its pathogenicity mechanisms have yet to be completely elucidated. In the present study, isolates of V. harveyi were isolated and characterized from diseased Penaeus monodon postlarvae from hatcheries in Kerala, India, from September to December 2016. All 23 tested isolates were positive for lipase, phospholipase, caseinase, gelatinase and chitinase activity, and 3 of the isolates (MFB32, MFB71 and MFB68) showed potential for significant biofilm formation. Based on the presence of virulence genes, the isolates of V. harveyi were grouped into 6 genotypes, predominated by vhpA+ flaB+ ser+ vhh1- luxR+ vopD- vcrD+ vscN-. One isolate from each genotype was randomly selected for in vivo virulence experiments, and the LD50 ranged from 1.7 ± 0.5 × 103 to 4.1 ± 0.1 × 105 CFU ml-1. The expression of genes during the infection in postlarvae was high in 2 of the isolates (MFB12 and MFB32), consistent with the result of the challenge test. However, in MFB19, even though all genes tested were present, their expression level was very low and likely contributed to its lack of virulence. Because of the significant variation in gene expression, the presence of virulence genes alone cannot be used as a marker for pathogenicity of V. harveyi.

Editorial

2017 ◽  
pp. 2-3
Author(s):  
Peter Hoar
Keyword(s):  
New Zealand ◽  
New Technologies ◽  
Editorial Team ◽  
Local Market ◽  
Design History ◽  
Recorded Sound ◽  
The Arts ◽  
The World ◽  
1960S And 1970S ◽  
New Zealanders

Kia ora and welcome to the second issue of BackStory. The members of the Backstory Editorial Team were gratified by the encouraging response to the first issue of the journal. We hope that our currentreaders enjoy our new issue and that it will bring others to share our interest in and enjoyment of the surprisingly varied backstories of New Zealand’s art, media, and design history. This issue takes in a wide variety of topics. Imogen Van Pierce explores the controversy around the Hundertwasser Art Centre and Wairau Māori Art Gallery to be developed in Whangarei. This project has generated debate about the role of the arts and civic architecture at both the local and national levels. This is about how much New Zealanders are prepared to invest in the arts. The value of the artist in New Zealand is also examined by Mark Stocker in his article about the sculptor Margaret Butler and the local reception of her work during the late 1930s. The cultural cringe has a long genealogy. New Zealand has been photographed since the 1840s. Alan Cocker analyses the many roles that photography played in the development of local tourism during the nineteenth century. These images challenged notions of the ‘real’ and the ‘artificial’ and how new technologies mediated the world of lived experience. Recorded sound was another such technology that changed how humans experienced the world. The rise of recorded sound from the 1890s affected lives in many ways and Lewis Tennant’s contribution captures a significant tipping point in this medium’s history in New Zealand as the transition from analogue to digital sound transformed social, commercial and acoustic worlds. The New Zealand Woman’s Weekly celebrates its 85th anniversary this year but when it was launched in 1932 it seemed tohave very little chance of success. Its rival, the Mirror, had dominated the local market since its launch in 1922. Gavin Ellis investigates the Depression-era context of the Woman’s Weekly and how its founders identified a gap in the market that the Mirror was failing to fill. The work of the photographer Marti Friedlander (1908-2016) is familiar to most New Zealanders. Friedlander’s 50 year career and huge range of subjects defy easy summary. She captured New Zealanders, their lives, and their surroundings across all social and cultural borders. In the journal’s profile commentary Linda Yang celebrates Freidlander’s remarkable life and work. Linda also discusses some recent images by Friedlander and connects these with themes present in the photographer’s work from the 1960s and 1970s. The Backstory editors hope that our readers enjoy this stimulating and varied collection of work that illuminate some not so well known aspects of New Zealand’s art, media, and design history. There are many such stories yet to be told and we look forward to bringing them to you.

Aflatoxins biosynthesis, toxicity and intervention strategies: A review

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Developing Countries ◽  
Molecular Basis ◽  
Genetic Regulation ◽  
Intervention Strategies ◽  
Historical Background ◽  
Economic Losses ◽  
Factors Affecting ◽  
Biomarkers Of Exposure ◽  
Food Commodities

Aflatoxins (AFTs) are toxic products of fungal metabolism, associated with serious health consequences and substantial economic losses to agriculture, livestock and poultry sectors, particularly in the developing countries. This review outlines the current information on AFTs in terms of historical background, classification, relative occurrence and co-existence with other mycotoxins in various food commodities. The phenomenon of aflatoxin (AFT) biosynthesis has been elucidated with reference to molecular basis, genetic regulation and factors affecting the AFT production. Moreover, the in vivo disposition kinetics, toxicological action and toxico-pathological consequences of AFTs have also been highlighted. Currently employed strategies for the detection and detoxification of AFTs, biomarkers of exposure assessment, potential economic impact and regulatory considerations regarding the AFTs have been emphasized.

Tautai

2017 ◽  
Author(s):  
Patricia O'Brien
Keyword(s):  
New Zealand ◽  
British Empire ◽  
League Of Nations ◽  
Late Nineteenth Century ◽  
World War ◽  
World War One ◽  
Indigenous Resistance ◽  
World Government ◽  
Pacific History ◽  
The World

This is a biography of Ta’isi O. F. Nelson, the Sāmoan nationalist leader who fought New Zealand, the British Empire and the League of Nations between the world wars. It is a richly layered history that weaves a personal and Pacific history with one that illuminates the global crisis of empire after World War One. Ta’isi’s story weaves Sweden with deep histories of Sāmoa that in the late nineteenth century became deeply inflected with colonial machinations of Germany, Britain, New Zealand and the U. S.. After Sāmoa was made a mandate of the League of Nations in 1921, the workings and aspirations of that newly minted form of world government came to bear on the island nation and Ta’isi and his fellow Sāmoan tested the League’s powers through their relentless non-violent campaign for justice. Ta’isi was Sāmoa’s leading businessman who was blamed for the on-going agitation in Sāmoa; for his trouble he was subjected to two periods of exile, humiliation and a concerted campaign intent on his financial ruin. Using many new sources, this book tells Ta’isi’s untold story, providing fresh and intriguing new aspects to the global story of indigenous resistance in the twentieth century.

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