scholarly journals Effect of Selenium Sources on Laying Performance, Egg Quality Characteristics, Intestinal Morphology, Microbial Population and Digesta Volatile Fatty Acids in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1681
Author(s):  
Aliyu Ibrahim Muhammad ◽  
Dalia Alla Mohamed ◽  
Loh Teck Chwen ◽  
Henny Akit ◽  
Anjas Asmara Samsudin
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Egg Quality ◽  
Basal Diet ◽  
Egg Yolk ◽  
Quality Characteristics ◽  
Intestinal Morphology ◽  
Treatment Groups ◽  
Laying Performance

The use of toxic and less bioavailable inorganic selenium can now be supplemented with an alternative organic source from bacterial species in nutrition for human and animal benefit. This study investigated the effects of selenium sources on laying performance, egg quality characteristics, intestinal morphology, caecum microbial population, and digesta volatile fatty acids in laying hens. One hundred and forty-four Lohman Brown Classic laying hens, at 23 weeks of age, were divided into four experimental groups (36 hens in each), differing in form of Se supplementation: no Se supplementation (Con), 0.3 mg/kg of inorganic Se in the form of sodium selenite (Na2SeO3), 0.3 mg/kg of organic Se from selenium yeast (Se-Yeast), and 0.3 mg/kg of organic Se from Stenotrophomonas maltophilia (bacterial organic Se, ADS18). The results showed that different dietary Se sources significantly affected laying rate, average egg weight, daily egg mass, feed conversion ratio (FCR), and live bodyweight (LBW) (p < 0.05). However, average daily feed intake and shell-less and broken eggs were unaffected (p > 0.05) among the treatment groups. The findings revealed that selenium sources had no (p > 0.05) effect on egg quality (external and internal) parameters. However, eggshell breaking strength and Haugh unit were significantly (p < 0.05) improved with organic (ADS18 or Se-yeast) Se-fed hens compared to the control group. In addition, egg yolk and breast tissue Se concentrations were higher (p < 0.05) in the dietary Se supplemented group compared to the control. Intestinal histomorphology revealed that hens fed ADS18 or Se-Yeast groups had significantly (p < 0.05) higher villi height in the duodenum and jejunum compared to those fed Na2SeO3 or a basal diet. However, when compared to organic Se fed (ADS18 or Se-Yeast) hens, the ileum villus height was higher (p < 0.05) in the basal diet group; with the lowest in the SS among the treatment groups. A significant increase (p < 0.05) of Lactobacilli spp. and Bifidobacteria spp., and a decrease of Escherichia coli and Salmonella spp. population were observed in the organic (ADS18 or Se-yeast) compared to inorganic supplemented and control hens. The individual digesta volatile fatty acid (VFA) was significantly different, but with no total VFA differences. Thus, bacterial selenoprotein or Se-yeast improved the performance index, egg quality characteristics, egg yolk and tissue Se contents, and intestinal villus height in laying hens. Moreover, caecum beneficial microbes increased with a decrease in the harmful microbe population and affected individual cecal volatile fatty acids without affecting the total VFA of the laying hens digesta.

scholarly journals Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 87
Author(s):  
Yoo-Bhin Kim ◽  
Sang-Hyeok Lee ◽  
Da-Hye Kim ◽  
Hyun-Gwan Lee ◽  
Yongjun Choi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Capacity ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Egg Quality ◽  
Inorganic Sulfur ◽  
Positive Effects ◽  
Laying Performance ◽  
Cont Group

The present study was conducted to investigate the comparative effects of organic and inorganic forms of sulfur, methyl sulfonyl methane (MSM) and sodium sulfate (SS), on laying performance, egg quality, ileal morphology, ileal volatile fatty acids, and antioxidant and stress markers in various biological samples in aged laying hens. A total of 144, 73-week-old Lohman Brown-Lite laying hens were randomly assigned to one of three experimental diets: basal diet (CONT), CONT + 0.2% MSM (MSM), and CONT + 0.3% SS (SS). The trial lasted for 12 weeks. MSM and SS diets contained 0.07% of sulfur, either organic or inorganic. Dietary MSM did not affect egg production or feed conversion ratio at 12 weeks compared with the CONT group. Dietary sulfur did not affect egg quality except for the Haugh unit at 4 weeks, which was lowered (p < 0.05) in the SS group. Compared with the CONT group, a higher (p < 0.05) villus height to crypt depth ratio was observed in the SS group. Dietary sulfur did not affect the percentages of short-chain fatty acids in the ileum. Total antioxidant capacity of the liver increased (p < 0.05) in laying hens fed MSM- and SS-added diets compared with the CONT group. The MSM and SS groups were found to have lowered (p < 0.05) malondialdehyde (MDA) concentration in serum samples compared with CONT. Finally, dietary MSM had the lowest (p < 0.05) MDA concentrations in yolk samples. Taken together, our study showed that dietary organic and inorganic sulfur have positive effects on ileal morphology and antioxidant capacity in laying hens. However, SS-mediated inhibition in laying performance needs to be clarified.

scholarly journals Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

2021 ◽  
Author(s):  
Yoo Bhin Kim ◽  
Sang Hyeok Lee ◽  
Da-Hye Kim ◽  
Hyun-Gwan Lee ◽  
Yongjun Choi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Capacity ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Egg Quality ◽  
Inorganic Sulfur ◽  
Positive Effects ◽  
Laying Performance ◽  
Cont Group

The present study was conducted to investigate the comparative effects of organic and inorganic forms of sulfur, methyl sulfonyl methane (MSM) and sodium sulfate (SS), on laying performance, egg quality, ileal morphology, ileal volatile fatty acids, and antioxidant and stress markers in various biological samples in aged laying hens. A total of 144, 73-week-old Lohman Brown-Lite laying hens were randomly assigned to one of three experimental diets: basal diet (CONT), CONT + 0.2% MSM (MSM), and CONT + 0.3% SS (SS). The trial lasted for 12 weeks. MSM and SS groups contained 0.07% of sulfur, either organic or inorganic. Dietary MSM did not affect egg production and feed conversion ratio at 12 weeks compared with the CONT group. Dietary sulfur did not affect egg quality except for Haugh unit at 4 weeks which was lowered (P &amp;lt; 0.05) in the SS group. Compared with the CONT group, higher (P &amp;lt; 0.05) villus height and crypt depth ratio was observed in the SS group. None of dietary sulfur affected the percentages of short-chain fatty acids in the ileum. Total antioxidant capacity of liver increased (P &amp;lt; 0.05) in laying hens fed MSM- and SS-added diets compared with the CONT group. The MSM and SS groups lowered (P &amp;lt; 0.05) malondialdehyde (MDA) concentration in serum samples compared with the CONT. Finally, dietary MSM had the lowest (P &amp;lt; 0.05) MDA concentrations in yolk samples. Taken together, our study showed that dietary organic and inorganic sulfur have positive effects on ileal morphology and antioxidant capacity in laying hens. However, SS-mediated inhibition in laying performance needs to be clarified.

scholarly journals Effect of Dietary Supplementation of Fermented Pine Needle Extract on Productive Performance, Egg Quality, and Serum Lipid Parameters in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1475
Author(s):  
Damini Kothari ◽  
Jong-Seok Oh ◽  
Ju-Hee Kim ◽  
Woo-Do Lee ◽  
Soo-Ki Kim
Keyword(s):  
Serum Lipid ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Egg Yolk ◽  
Egg Mass ◽  
Productive Performance ◽  
Laying Performance

This study aimed to investigate the supplemental effects of fermented pine (Pinus densiflora) needle extract (FPNE) in laying hen diets on productive performance, egg quality, and serum lipid metabolites. A total of 108 40-week-old Hy-line brown laying hens were randomly assigned to one of the three dietary treatment groups: (1) basal diet + 0 mL FPNE/kg diet (CON), (2) basal diet + 2.5 mL FPNE/kg diet (T1), or (3) basal diet + 5 mL FPNE/kg diet (T2) for 6 weeks. Each group consisted of four replicates of nine hens each. Feed and water provided ad libitum. Results showed that dietary supplementation of FPNE increased egg production percentage (linear, p < 0.01 and quadratic, p < 0.05), egg mass (linear, p < 0.05), and feed intake (linear, p < 0.05) during the entire experimental period. In addition, dietary inclusion of FPNE significantly increased the eggshell color (linear, p < 0.01), egg yolk color (quadratic, p < 0.01), and eggshell breaking strength (linear, p < 0.05 and quadratic, p < 0.05) while the Haugh unit decreased (quadratic, p < 0.05). However, serum lipid profile did not differ among the dietary treatments (p > 0.05). Notably, antioxidant activity of egg yolk was improved by significantly decreasing the malondialdehyde content in egg yolks after 6 weeks of storage (linear, p < 0.001 and quadratic, p < 0.05). In summary, dietary inclusion of FPNE could improve laying performance and the antioxidant capacity of eggs.

scholarly journals Laying Performance, Physical, and Internal Egg Quality Criteria of Hens Fed Distillers Dried Grains with Solubles and Exogenous Enzyme Mixture

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 150 ◽  
Author(s):  
Mohamed E. Abd El-Hack ◽  
Khalid M. Mahrose ◽  
Faten A. M. Attia ◽  
Ayman A. Swelum ◽  
Ayman E. Taha ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Egg Production ◽  
Unsaturated Fatty Acids ◽  
Egg Quality ◽  
Egg Yolk ◽  
Exogenous Enzyme ◽  
Distillers Dried Grains ◽  
Enzyme Mixture ◽  
Laying Performance ◽  
Enzyme Cocktail

: The effects of dietary inclusion of distillers dried grains with solubles in laying hen diets with and without exogenous enzyme mixture (EEM) on performance and egg characteristics were evaluated. One of the main objectives of this study was to examine the effects of distillers dried grains with solubles (DDGS) and enzyme cocktail on egg yolk fatty acids. The study used total of 144 Hisex Brown laying hens in a 4 × 2 factorial arrangement, including four levels of DDGS (0, 6, 12, and 18% of diet) and two levels of enzyme cocktail (0 or 250 mg EEM/kg of diet) through 32–42 weeks of the age. The inclusion of 18% DDGS was associated with the worst (p ≤ 0.001) egg production and the lowest daily feed intake. Numerically, hens fed 6% DDGS diet consumed more feed and had the greatest egg production. The best feed conversion ratio (FCR) was recorded in the control, while the worst was recorded in the 18% DDGS group. Compared with EEM-free diets, EEM supplementation improved FCR by about 2.79%, but the difference was not significant. Shell thickness and shell percentage were significantly increased in hens fed 6% DDGS diet compared to other groups. Egg weights in the 6% and 12% DDGS groups were significantly higher than those in the control and 18% DDGS groups. Non-significant increases in shell and albumin percentages were recorded in groups fed EEM-supplemented diets. The interaction effect of DDGS and EEM was significant (p ≤ 0.01) for the majority of egg characteristics. As dietary DDGS level increased, yolk color density increased. Elevating DDGS level up to 18% increased yolk cholesterol, total fat, and total unsaturated fatty acids. The effects of EEM supplementation on egg yolk fatty acid composition and the interaction effects between DDGS and EEM were not significant. Considering these results, it could be concluded that DDGS is an acceptable feed ingredient in layer diets and that the maximum inclusion level of DDGS should not exceed 12% of the whole diet.

scholarly journals Effects of Ginger Extract on Laying Performance, Egg Quality, and Antioxidant Status of Laying Hens

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 857 ◽  
Author(s):  
Wen ◽  
Gu ◽  
Tao ◽  
Cheng ◽  
Wang ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Laying Hens ◽  
Egg Quality ◽  
Basal Diet ◽  
Cholesterol Content ◽  
Ginger Extract ◽  
Sod Activity ◽  
Egg Weight ◽  
Laying Performance ◽  
Yolk Cholesterol

The objective of this study was to investigate the effects of ginger extract (GE) as a dietary supplement for laying hens. A total of 40-week-old 288 Hyline Brown laying hens were randomly divided into two groups with six replicates, and fed a basal diet with or without 100 g/t GE for eight weeks. Dietary GE supplementation increased egg weight, albumin height, and Haugh unit of eggs, and decreased yolk cholesterol content and activities of alanine transaminase and aspartate transaminase in serum at eight weeks. Moreover, GE resulted in higher total superoxide dismutase (T-SOD) activity and lower malondialdehyde (MDA) content in yolk at four and eight weeks and in serum. It was concluded that GE was effective in increasing egg weight and improving the egg quality and antioxidant status of laying hens.

scholarly journals Dietary cadmium chloride impairs shell biomineralization by disrupting the metabolism of the eggshell gland in laying hens

2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Mingkun Zhu ◽  
Huaiyu Li ◽  
Liping Miao ◽  
Lanlan Li ◽  
Xinyang Dong ◽  
...  
Keyword(s):  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Complement C3 ◽  
Mrna Levels ◽  
Palisade Layer ◽  
Positive Effects ◽  
Laying Performance ◽  
Dietary Cadmium

Abstract In this study, we identified cadmium (Cd) as a potential endocrine disruptor that impairs laying performance, egg quality, and eggshell deposition and induces oxidative stress and inflammation in the eggshell glands of laying hens. A total of 480 38-wk-old laying hens were randomly assigned into 5 groups that were fed a basal diet (control) or a basal diet supplemented with Cd (provided as CdCl2·2.5 H2O) at 7.5, 15, 30, and 60 mg Cd per kg feed for 9 wk. The results showed that, when compared with the control group, a low dose of dietary Cd (7.5 mg/kg) had positive effects on egg quality by improving albumen height, Haugh unit, yolk color, and shell thickness at the third or ninth week. However, with the increase in the dose and duration of Cd exposure, the laying performance, egg quality, and activities of eggshell gland antioxidant enzymes (catalase [CAT], glutathione peroxide [GSH-Px]), and ATPase (Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase) deteriorated, and the activity of total nitric oxide synthase (T-NOS) and the level of malondialdehyde (MDA) increased significantly (P &lt; 0.05). The histopathology and real-time quantitative PCR results showed that Cd induced endometrial epithelial cell proliferation accompanied by upregulation of the mRNA levels of progesterone receptor (PgR) and epidermal growth factor receptor (EGFR), downregulation of the mRNA levels of estrogen receptor α (ERα) and interleukin 6 (IL6), and inflammation of the eggshell gland accompanied by significantly increased expression of complement C3 and pro-inflammatory cytokine tumor necrosis factor α (TNFα) (P &lt; 0.05). In addition, the ultrastructure of the eggshell showed that dietary supplementation with 7.5 mg/kg Cd increased the palisade layer and total thickness of the shell, but with the increase in dietary Cd supplementation (30 and 60 mg/kg) the thickness of the palisade layer and mammillary layer decreased significantly (P &lt; 0.05), and the outer surface of the eggshell became rougher. Correspondingly, the expression of calbindin 1 (CALB1), ovocalyxin-32 (OCX-32), ovocalyxin-36 (OCX-36), osteopontin (SPP1), and ovocledidin-17 (OC-17) decreased significantly (P &lt; 0.05) with increasing dietary Cd supplementation. Conclusively, the present study demonstrates that dietary supplementation with Cd negatively affects laying performance, egg quality, and eggshell deposition by disturbing the metabolism of eggshell glands in laying hens but has a positive effect on egg quality at low doses.

scholarly journals Performance of Laying Hens Fed Sorghum Based Diets Supplemented with Enzymes

2021 ◽  
Vol 43 (1) ◽  
pp. 224-232
Author(s):  
O. M. Agunbiade ◽  
P. A. Onimisi ◽  
J. J. Omage
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Quality Characteristics ◽  
Egg Laying ◽  
The Other ◽  
Control Treatment ◽  
Feed Cost ◽  
Laying Performance ◽  
Dietary Treatments

Sorghum is the primary alternative feedstuff for corn in some developed countries in the production of poultry. Thus, four hundred and fifty 32 weeks old Lohmann Brown laying hens were used in a study to evaluate the effect of replacing maize with sorghum with or without enzymes supplementation, on egg laying performance and egg quality characteristics of laying hens. Birds were distributed randomly into six dietary treatments with three replicates per treatment, each replicate had 25 birds with uniform initial group weights. The treatments included maize without enzymes (T1); sorghum without enzymes (T2); as controls respectively, while T3, T4, T5, and T6 contained sorghum based diets with phytase, protease, G2G, and a combination of protease and G2G respectively. Laying performance, cost of production, and egg quality characteristics were determined for the period of the trial, 33-56 weeks of age of the birds. All data obtained were subjected to analysis of variance, using the completely randomized design (CRD) and significant differences among treatment means were compared using the Tukey test. Significant (p<0.05) differences were observed among dietary treatments but with no defined trend observed for egg production traits. Daily feed intake was significantly (p<0.05) higher in maize control diet compared to the other treatments. Diets supplemented with phytase (T3), protease (14), and combination of protease and roxazyme G2G (T6) had significantly (p<0.05) better feed conversion ratio with T3 having the lowest value of 4.01 compared to the other treatments. For feed cost/dozen egg (N), Kg feed/dozen eggs, income above feed cost at N30 per egg, and income above control treatment; phytase (T3), protease (T4), roxazyme 626 (T5), and protease + roxacyme G2G (T6) diets in this study showed better performance than the control. Sorghum diet without enzyme had significantly (p<0.05) least performance for egg number. Hen housed egg production (HHP) and hen day egg production (HDP) compared to the other treatments while sorghum diet with phytase had the highest value (60.12g/day) for average egg weight compared to the other treatments. Egg quality characteristics showed the best performance for dietary treatments supplemented with phytase (T3) in all the other parameters considered, apart from the albumen height, yolk diameter, and yolk index. It was concluded therefore, that sorghum with phytase and protease supplementations can be used in layers' diets as a substitute for maize for optimum egg production.

scholarly journals Effects of feed supplementation with manganese from its different sources on performance and egg parameters of laying hens

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Hydrolysate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Feed Supplementation ◽  
Positive Effects ◽  
Dietary Treatments

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;

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