scholarly journals Effect of Dietary Supplementation of Fermented Pine Needle Extract on Productive Performance, Egg Quality, and Serum Lipid Parameters in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1475
Author(s):  
Damini Kothari ◽  
Jong-Seok Oh ◽  
Ju-Hee Kim ◽  
Woo-Do Lee ◽  
Soo-Ki Kim

This study aimed to investigate the supplemental effects of fermented pine (Pinus densiflora) needle extract (FPNE) in laying hen diets on productive performance, egg quality, and serum lipid metabolites. A total of 108 40-week-old Hy-line brown laying hens were randomly assigned to one of the three dietary treatment groups: (1) basal diet + 0 mL FPNE/kg diet (CON), (2) basal diet + 2.5 mL FPNE/kg diet (T1), or (3) basal diet + 5 mL FPNE/kg diet (T2) for 6 weeks. Each group consisted of four replicates of nine hens each. Feed and water provided ad libitum. Results showed that dietary supplementation of FPNE increased egg production percentage (linear, p < 0.01 and quadratic, p < 0.05), egg mass (linear, p < 0.05), and feed intake (linear, p < 0.05) during the entire experimental period. In addition, dietary inclusion of FPNE significantly increased the eggshell color (linear, p < 0.01), egg yolk color (quadratic, p < 0.01), and eggshell breaking strength (linear, p < 0.05 and quadratic, p < 0.05) while the Haugh unit decreased (quadratic, p < 0.05). However, serum lipid profile did not differ among the dietary treatments (p > 0.05). Notably, antioxidant activity of egg yolk was improved by significantly decreasing the malondialdehyde content in egg yolks after 6 weeks of storage (linear, p < 0.001 and quadratic, p < 0.05). In summary, dietary inclusion of FPNE could improve laying performance and the antioxidant capacity of eggs.

2020 ◽  
Vol 8 (2) ◽  
pp. 93-99
Author(s):  
A.R. Zhang ◽  
S.A. Pirzado ◽  
G.H. Liu ◽  
Z.M. Chen ◽  
W.H. Chang ◽  
...  

The aim of this study was to evaluate the influence of the dietary addition of sodium humate (HAS) on performance and immune response of laying hens during peak lay, over an eight-week period. Laying hens, aged 24 weeks old, were randomly allotted to four dietary treatments: a basal control diet or the basal diet supplemented with 0.1, 0.3 or 0.5% HAS. Each treatment had six replicates with 15 layers per replicate. Egg production, egg quality, serum antioxidant parameters and immunity indexes were measured. HAS had no significant effect on egg production or egg and shell quality, but all supplemental levels significantly improved Haugh unit values and egg yolk colour. Supplementation with all levels of HAS significantly increased serum immunoglobuline (Ig) level compared to the hens fed the control diet. Feeding either 0.1 or 0.5% HAS significantly increased serum IgM levels. The results indicated that dietary supplementation with HAS can enhance egg albumin quality and improve immunity in laying hens.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 354-355
Author(s):  
Jomari B Delos Reyes ◽  
Jong Hyuk Kim ◽  
Geun Hyeon Park ◽  
Hyeon Seok Choi ◽  
Gi Ppeum Han ◽  
...  

Abstract Endogenous synthesis of vitamin C may satisfy its requirement for laying hens; however, some previous experiments showed beneficial effects of dietary supplementation of vitamin C in laying hens. Thus, the results are still controversial. The objective of the current experiment, therefore, was to investigate the effect of vitamin C supplementation in diets on productive performance, egg quality, relative organ weight, and tibia strength in laying hens. A total of 504 46-wk-old Hy-Line Brown laying hens were allotted to 1 of 6 dietary treatments with 7 replicates in a completely randomized design. Each replicate had 12 hens. Diets were prepared by supplementing the basal diet with vitamin C at the levels of 250, 500, 1,000, 2,000, or 3,000 mg/kg and were fed to hens for 6 wks. Results indicated that increasing supplementation of vitamin C in diets increased hen-day egg production and egg mass (quadratic, P &lt; 0.05), but decreased production of broken or soft-shell eggs (linear and quadratic, P &lt; 0.01) and feed conversion ratio (quadratic, P &lt; 0.05). These positive effects were observable at the supplementation level of 250 mg/kg vitamin C, but there were no further benefits at the greater levels of vitamin C. Increasing supplementation of vitamin C in diets had no effects on egg quality, except for a decrease (quadratic, P &lt; 0.01) in egg yolk color with increasing vitamin C supplementation. The relative weights of the liver, kidney, and spleen were not affected by increasing supplementation of vitamin C in diets. Tibia strength was not influenced by increasing supplementation of vitamin C. In conclusion, dietary supplementation of vitamin C has a beneficial effect on egg production of laying hens. Quadratic responses of increasing vitamin C supplementation in diets reveal that supplementation of 250 mg/kg vitamin C is recommended for diets fed to laying hens at 46–52 wks of age.


2019 ◽  
Vol 18 (03) ◽  
pp. 41-47
Author(s):  
Tung M. Che

The objective of the experiment was to evaluate effects of dietary supplementation of β-mannanase (Hemicell®) on productive performance, egg quality, and fecal moisture content in laying hens from 20 to 35 weeks of age. A total of 375 Isa Brown’s hens (1615.6 ± 76.4 g/bird) were randomly assigned to 5 treatments in a completely randomized design. The 5 dietary treatments included (1) basal diet with a level of 2800 kcal ME and no β-mannanase supplementation (HE, Control), (2) HE + 32 units of β-mannanase/g of feed, (3) HE + 64 units of β-mannanase/g of feed, (4) basal diet with a level of 2700 kcal ME (LE) + 32 units of β-mannanase/g of feed, and (5) LE + 64 units of β-mannanase/g of feed. Each treatment was replicated with 25 cages of 3 hens each. All diets were in meal form and contained no antibiotics. The addition of β-mannanase to HE diets did not affect the egg production of birds as compared with the control (P > 0.05). The birds fed LE diets with β-mannanase had the same egg production as those fed the control and β-mannanase-supplemented HE diets (P > 0.05). Differences in egg weight, egg quality, survival rate, and fecal moisture content were not significant among the treatments (P > 0.05). Briefly, addition of β-mannanase (32 units/g of feed) to LE diets would be beneficial for layers during the early laying period as it resulted in the same performance and egg quality as the HE diets without β-mannanase supplementation.


2020 ◽  
Vol 100 (3) ◽  
pp. 411-417
Author(s):  
Huan Shi ◽  
Wan Lin Zhang ◽  
In Ho Kim

An experiment was carried out to investigate the effect of Bacillus subtilis RX7 and B2A supplementation on egg production, egg quality, blood profile, and excreta Salmonella population in laying hens. A total of 192 Hy-Line Brown laying hens (40-wk-old) were randomly assigned into four dietary treatments, each of which was replicated eight times with six hens per replicate in a 5 wk trial. The dietary treatments were NC, which is a basal diet without antibiotics or Bacillus subtilis; PC, which is an NC + 0.5 g kg−1 antibiotic (virginiamycin) diet; BSR, which is an NC + 0.5 g kg−1 Bacillus subtilis RX7 (1.0 × 109 colony forming units g−1); and BSB, which is an NC + 0.5 g kg−1 Bacillus subtilis B2A (1.0 × 109 colony forming units g−1). The results showed that hens receiving BSB diets had increased (P < 0.05) eggshell thickness over hens fed the NC, PC, and BSR diets (week 3). The excreta Salmonella counts in the PC, BSR, and BSB groups were lower than the NC group (P < 0.05). These results indicate that dietary inclusion of Bacillus subtilis RX7 and B2A strains effectively decreased excreta Salmonella counts without any detrimental effects on the performance of laying hens.


2019 ◽  
Vol 59 (8) ◽  
pp. 1553 ◽  
Author(s):  
S. S. Diarra ◽  
I. Wamekeni ◽  
A. Vunagilee ◽  
H. Lavaka ◽  
T. Finau

The effects of peeling and limestone flour level on the utilisation of Alocasia macrorrhiza corm meal (ACM) by laying hens were investigated. Maize-based diets with two concentrations of limestone flour (40 and 60 g/kg) and diets containing 200 g peeled and unpeeled ACM meal/kg with the same levels of limestone flour were fed to five replicate pens containing 10 20-week-old Shaver brown pullets (1635 ± 148 g), each for 105 days. Results showed no interaction or main effects of ACM or limestone concentration on feed intake, egg weight, egg mass, feed conversion efficiency, egg shape index, surface area and Haugh unit (P &gt; 0.05). Feeding whole ACM with 40 g limestone/kg diet reduced hen-day production and shell thickness (P &lt; 0.05) but this was overcome by increasing limestone concentration to 60 g/kg diet. Peeled ACM with both concentrations of limestone flour maintained laying performance and egg quality. We concluded that feeding 200 g whole ACM depresses egg production and shell quality in laying hens, but increasing dietary limestone concentration overcomes this adverse effect. The concentration of limestone flour in the diet has no effect on laying performance. Higher inclusion levels of ACM, calcium source and level that will maintain laying performance and reduce feed cost need to be investigated.


2020 ◽  
Vol 98 (2) ◽  
Author(s):  
Mingkun Zhu ◽  
Huaiyu Li ◽  
Liping Miao ◽  
Lanlan Li ◽  
Xinyang Dong ◽  
...  

Abstract In this study, we identified cadmium (Cd) as a potential endocrine disruptor that impairs laying performance, egg quality, and eggshell deposition and induces oxidative stress and inflammation in the eggshell glands of laying hens. A total of 480 38-wk-old laying hens were randomly assigned into 5 groups that were fed a basal diet (control) or a basal diet supplemented with Cd (provided as CdCl2·2.5 H2O) at 7.5, 15, 30, and 60 mg Cd per kg feed for 9 wk. The results showed that, when compared with the control group, a low dose of dietary Cd (7.5 mg/kg) had positive effects on egg quality by improving albumen height, Haugh unit, yolk color, and shell thickness at the third or ninth week. However, with the increase in the dose and duration of Cd exposure, the laying performance, egg quality, and activities of eggshell gland antioxidant enzymes (catalase [CAT], glutathione peroxide [GSH-Px]), and ATPase (Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase) deteriorated, and the activity of total nitric oxide synthase (T-NOS) and the level of malondialdehyde (MDA) increased significantly (P &lt; 0.05). The histopathology and real-time quantitative PCR results showed that Cd induced endometrial epithelial cell proliferation accompanied by upregulation of the mRNA levels of progesterone receptor (PgR) and epidermal growth factor receptor (EGFR), downregulation of the mRNA levels of estrogen receptor α (ERα) and interleukin 6 (IL6), and inflammation of the eggshell gland accompanied by significantly increased expression of complement C3 and pro-inflammatory cytokine tumor necrosis factor α (TNFα) (P &lt; 0.05). In addition, the ultrastructure of the eggshell showed that dietary supplementation with 7.5 mg/kg Cd increased the palisade layer and total thickness of the shell, but with the increase in dietary Cd supplementation (30 and 60 mg/kg) the thickness of the palisade layer and mammillary layer decreased significantly (P &lt; 0.05), and the outer surface of the eggshell became rougher. Correspondingly, the expression of calbindin 1 (CALB1), ovocalyxin-32 (OCX-32), ovocalyxin-36 (OCX-36), osteopontin (SPP1), and ovocledidin-17 (OC-17) decreased significantly (P &lt; 0.05) with increasing dietary Cd supplementation. Conclusively, the present study demonstrates that dietary supplementation with Cd negatively affects laying performance, egg quality, and eggshell deposition by disturbing the metabolism of eggshell glands in laying hens but has a positive effect on egg quality at low doses.


2018 ◽  
Vol 34 (2) ◽  
pp. 71-78
Author(s):  
M Ahammed ◽  
S Sharmin ◽  
A Khatun ◽  
KMS Islam

A total of 96 Shaver 579 laying hens of 78 weeks of age were studied for the effects on egg production of dietary supplementation with spirulina (Spirulina platensis). The hens were divided into four dietary treatments with six replications of four birds in each group. Birds of three groups were supplemented with 0.1%, 0.2% and 0.3% spirulina and one group was kept as control. Yolk colour significantly improved (P<0.01) as compared to control, without affecting any other production performances. It is suggested that dietary supplementation of spirulina at 0.2 to 0.3% levels in aged laying hens enhanced egg yolk colour. Bangl. vet. 2017. Vol. 34, No. 2, 71-78


2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;


Author(s):  
Yang Jiao ◽  
Rajesh Jha ◽  
Wan Lin Zhang ◽  
In Ho Kim

The present study evaluated the effects of dietary supplementation of chitooligosaccharide (COS) on egg production, egg quality and blood profiles in laying hens. A total of 192 Hy-Line Brown laying hens (initial age- 46 weeks) were allocated to one of four treatments with eight replications per treatment and six adjacent cages as replication for eight-weeks study. Dietary treatments consisted of CON, basal diet; COS1, CON + 0.1% COS; COS2, CON + 0.2% COS; and COS3, CON + 0.3% COS. Linear improvement (p Lass Than 0.05) was observed in egg quality and high-density lipoprotein cholesterol (HDL/C) concentration at subsequent weeks. In week 8, linear (P=0.04) improvement was observed in haugh unit of egg with the increase in the dose of COS. In addition, linear effects (p Lass Than 0.05) on eggshell thickness at week 3, 5, 6, 7 and 8 were detected as the level for increased COS. In conclusion, the supplementation of COS in laying hens diets linearly improved the egg quality and the HDL/C concentration. Thus, COS has the potential to be used as a substitute to antibiotics in layer hen’s diets.


Author(s):  
Zabihollah Nemati ◽  
Zahra Moradi ◽  
Kazem Alirezalu ◽  
Maghsoud Besharati ◽  
António Raposo

Medicinal plants with antibacterial effects have been used by humans for centuries. In the recent decade, due to the development of antibiotic resistant strains, many studies have focused on the use of natural compounds as feed additives in livestock. Ginger, among all, have repetitively shown numerous biological activities, antibacterial, and antibiotic properties. This study was conducted to evaluate the effects of ginger root powder (GP) on the performance, egg quality, and blood parameters of Japanese quail. A total of 240 10-weeks old female quails were used in a completely randomized design with 4 treatments, 4 replicates, and 15 birds per replicate. Dietary treatment were basal diet (control) and basal diet containing 0.5, 1, and 1.5 g/kg of ginger root powder. Growth performance and exterior and interior quality of egg were measured biweekly over eight-week period. At the end of experiment blood parameters were evaluated. The results showed that diet supplementation with different levels of GP had no significant effect on egg production, egg mass weight, and egg weight (p > 0.05). However, feed intake and feed conversion ratio were significantly lower in the treatment group than the control in the whole period (p < 0.05). Egg Quality traits (shape index, albumen index, the percentage of albumen, yolk and shell, yolk pH, and shell thickness and strength) were not affected by the supplements in the whole trial period. Addition of GP significantly increased the albumen height, Haugh unit, and albumen pH in comparison with the control treatment (p < 0.05). GP reduced blood triglyceride level yet was ineffective on blood total antioxidant capacity and malondialdehyde. In conclusion, dietary supplementation with GP, could improve productive performance and the egg quality of Japanese quails. Nonetheless a comprehensive study needs to be performed in order to evaluate the impact of quail dietary ginger supplementation on productive performance and egg quality and their stability during storage time for commercial use.


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