scholarly journals Transport and Recovery of Turbot (Scophthalmus maximus) Sedated with MS-222 and Eugenol: Effects on Intermediary Metabolism and Osmoregulation

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2228
Author(s):  
Jie Cao ◽  
Qi Wang ◽  
Weiqiang Qiu ◽  
Jun Mei ◽  
Jing Xie

This study focused on the anesthetic waterless keep-alive transport technique for turbot. MS-222 and eugenol were used to anesthetize turbot and then the waterless keep-alive transport was conducted. The blood physiological changes and flesh quality changes of turbot were evaluated after cooling and during the simulated waterless transport. The results show that the temperature lowered from 13 to 2 °C, resulting in a decrease in moisture, fat and protein contents of all samples. Compared to the control turbot, the turbots treated with MS-222 and eugenol presented higher pH and glycogen content. During the simulated waterless transport, the pH, ATP and glycogen contents in MS-222- and eugenol-treated turbots decreased and the IMP and lactate levels increased. For the blood biochemical indices, blood glucose, cortisol and urea nitrogen increased with the increase in transport time in MS-222- and eugenol-treated turbots. At sampling time, the changes in blood physiological indices were significantly higher in the control samples than those in the MS-222- and eugenol-treated samples. The results indicate that the turbot samples treated with MS-222 or eugenol could reduce stress during cooling and simulated waterless transport.

Author(s):  
Jiangpeng Wu ◽  
Yifan Yang ◽  
Long Cheng ◽  
Jing Wu ◽  
Lili Xi ◽  
...  

Abstract Gastric cancer (GC) continues to be one of the major causes of cancer deaths worldwide. Meanwhile, liquid biopsies have received extensive attention in the screening and detection of cancer along with better understanding and clinical practice of biomarkers. In this work, 58 routine blood biochemical indices were tentatively used as integrated markers, which further expanded the scope of liquid biopsies and a discrimination system for GC consisting of 17 top-ranked indices, elaborated by random forest method was constructed to assist in preliminary assessment prior to histological and gastroscopic diagnosis based on the test data of a total of 2951 samples. The selected indices are composed of eight routine blood indices (MO%, IG#, IG%, EO%, P-LCR, RDW-SD, HCT and RDW-CV) and nine blood biochemical indices (TP, AMY, GLO, CK, CHO, CK-MB, TG, ALB and γ-GGT). The system presented a robust classification performance, which can quickly distinguish GC from other stomach diseases, different cancers and healthy people with sensitivity, specificity, total accuracy and area under the curve of 0.9067, 0.9216, 0.9138 and 0.9720 for the cross-validation set, respectively. Besides, this system can not only provide an innovative strategy to facilitate rapid and real-time GC identification, but also reveal the remote correlation between GC and these routine blood biochemical parameters, which helped to unravel the hidden association of these parameters with GC and serve as the basis for subsequent studies of the clinical value in prevention program and surveillance management for GC. The identification system, called GC discrimination, is now available online at http://lishuyan.lzu.edu.cn/GC/.


2019 ◽  
Vol 27 (4) ◽  
pp. 2321-2328
Author(s):  
Mohamed Sallam ◽  
Ibrahim El Wardany ◽  
Sayed Abdel-Fattah ◽  
Gamal El Mallah ◽  
Saad El Din Yassein

2012 ◽  
Vol 32 (5) ◽  
pp. 584-590 ◽  
Author(s):  
Hae-Chang Yi ◽  
Mi-Young Kim ◽  
Chang-Sun Choi ◽  
Young-Nam Kim ◽  
Chan-Kyu Han ◽  
...  

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