Exploring the Biotechnological Value of Marine Invertebrates: A Closer Look at the Biochemical and Antioxidant Properties of Sabella spallanzanii and Microcosmus squamiger

Sabella spallanzanii and Microcosmus squamiger were profiled for proximate composition, minerals, amino acids, fatty acids (FA), carotenoids, radical scavenging activity on the 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical, oxygen radical absorbance capacity (ORAC) and iron and copper chelating properties. Microcosmus squamiger had the highest level of moisture and crude protein, S. spallanzanii was enriched in crude fat and ash. Both species had similar levels of carbohydrates and energy. There was a prevalence of arginine and glycine in S. spallanzanii, and of taurine in M. squamiger. The most abundant minerals in both species were Na, Ca, and K. The methanol extract of S. spallanzanii had metal chelating properties towards copper and iron, while the methanol extract of M. squamiger was able to chelate copper. M. squamiger extracts had similar ORAC values. Fucoxanthinol and fucoxanthin were the major carotenoids in the M. squamiger dichloromethane extract. Saturated FA were more abundant than unsaturated ones in methanol extracts, and unsaturated FA prevailed in the dichloromethane extracts. Palmitic acid was the predominant FA in methanol extracts, whereas eicosapentaenoic (EPA) and dihomo-γ-linolenic acids were the major compounds in dichloromethane extracts. Low n-6/n-3 ratios were obtained. Our results suggests that both species could be explored as sources of bioactive ingredients with multiple applications.

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Total Flavonoids, Total Phenolic Content and Antioxidant Activity from Fruits, Leaves, Twigs and Flowers of Mesua ferrea L.

Walailak Journal of Science and Technology (WJST) ◽

10.48048/wjst.2018.3946 ◽

2018 ◽

Vol 15 (4) ◽

pp. 295-304

Author(s):

Prakit CHAITHADA ◽

Juthamas SUPAPAN ◽

Phetcharat RODTHUK ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Total Flavonoids ◽

Dpph Radical ◽

Methanol Extracts ◽

Crude Extracts ◽

Dpph Radical Scavenging

The objective of this study was to compare antioxidant activity from crudes of fruits, leaves, twigs and flowers of Mesua ferrea L. with hexane, dichloromethane and methanol by controlling time and temperature in the solvent extraction. All crude extracts were investigated for their antioxidant capacity in 1,1-diphenenyl-2-picryhydrazyl (DPPH) radical scavenging. The results indicated that the high polar solvent exhibited the highest DPPH radical scavenging activity. The methanol extracts of leaves show the highest activity with IC50 of 89.94±0.65 μg/mL. The flowers, twigs and fruits methanol crude extracts show IC50 of 94.26±5.93, 94.45±1.67 and 722.94±5.85 μg/mL, respectively. The methanol extracts of leaves also had the highest total flavonoids content (325.79±3.08mgQE/g). The methanol extract of flowers and the dichloromethane extract of fruits presented higher total phenolic contents than the other extracts which contained 769.11±46.64 mgGAE/g and 703.62±12.62 mgGAE/g, respectively, followed by the methanol extract of leaves (348.36±38.53 mgGAE/g).

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Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2018/v3i229823 ◽

2018 ◽

pp. 1-11

Author(s):

Iserhienrhien Lucky Osafanme ◽

Okolie Paulinus Ngozi

Keyword(s):

Aqueous Extract ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Scientific Basis ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

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Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Hemijska industrija ◽

10.2298/hemind160518047g ◽

2017 ◽

Vol 71 (5) ◽

pp. 361-370

Author(s):

Slavica Grujic ◽

Ana Dzamic ◽

Violeta Mitic ◽

Vesna Stankov-Jovanovic ◽

P.D. Marin ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Radical Scavenger ◽

Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

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A Comparative Study on Antioxidant Properties of Two Phyllanthus Species Growing in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v11i2.14579 ◽

2013 ◽

Vol 11 (2) ◽

pp. 191-197 ◽

Cited By ~ 4

Author(s):

Ishrat Nimmi ◽

Ismet Ara Jahan ◽

M Hemayet Hossain ◽

Mohammad Burhan Uddin ◽

M Sohel Rana ◽

...

Keyword(s):

Comparative Study ◽

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Test Methods ◽

Total Phenolic ◽

Solvent Volume

A comparative study of antioxidant properties on two varieties of Phyllanthus (Phyllanthus niruiri L. & Phyllanthus urinaria L. leaves) growing in Bangladesh was done in the present study. Five complimentary test methods namely DPPH free radical scavenging activity, reducing power assay, total antioxidant capacity, total phenolic and flavonoid contents determination were used for the evaluation of antioxidant properties of two species of Phyllanthus. In the present investigation for the evaluation of antioxidant activities the concentration is expressed by the ratio of crude sample per solvent volume instead of extract weight per solvent volume. Based on these concentrations the investigated P. niruri L. and P. urinaria L. leaves extracts exhibited significant results. At 2 mg/ml concentration DPPH radical scavenging capacity of the methanol extract of both the plants was found to show significant (>90%) activity which is comparable to Ascorbic acid and BHA. In case of reducing power tests for both the extract, the activity of P. urinaria is comparable to that of BHA and AA and that of P. niruiri is comparable to BHA. P. niruiri showed better antioxidant potential than that of P. urinaria based on the observed results of their corresponding methanol extract. DOI: http://dx.doi.org/10.3329/dujps.v11i2.14579 Dhaka Univ. J. Pharm. Sci. 11(2): 191-197, 2012 (December)

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STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF SAXICOLOUS LICHEN $ \textit{XANTHOPARMELIA STENOPHYLLA} $

Proceedings of the YSU B: Chemical and Biological Sciences ◽

10.46991/pysu:b/2020.54.2.132 ◽

2020 ◽

Vol 54 (2 (252)) ◽

pp. 132-137

Author(s):

A.G. Simonyan ◽

R.R. Sargsyan ◽

H.H. Panosyan ◽

A.H. Trchounian

Keyword(s):

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Dpph Radical ◽

Dpph Radical Scavenging Activity ◽

Gram Positive Bacteria ◽

Acetone Extracts ◽

Dpph Radical Scavenging

Antibacterial and antioxidant activities of different extracts of saxicolous lichen Xanthoparmelia stenophylla sampled from Norashen, Gegharkunik Province of Armenia were studied. Methanol, ethanol and acetone extracts of lichen thalli were demonstrated to have activity against only tested gram-positive bacteria. Methanol extract of the lichen showed the highest amount of DPPH radical scavenging activity (~68%). Our studies did not reveal any significant antibacterial and antioxidant activities of aqueous extract.

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In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

BioMed Research International ◽

10.1155/2018/1824790 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Gauhar Rehman ◽

Muhammad Hamayun ◽

Amjad Iqbal ◽

Saif Ul Islam ◽

Saba Arshad ◽

...

Keyword(s):

Glucose Uptake ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Yeast Cells ◽

In Vitro Assays ◽

Dpph Radical ◽

Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

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Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i12.2749-2755.4038 ◽

2020 ◽

Vol 8 (12) ◽

pp. 2749-2755

Author(s):

Mohammad Afzal Hossain ◽

Nadia Khan Disha ◽

Jahid Hasan Shourove ◽

Pappu Dey

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanol Extract ◽

Scavenging Activity ◽

Dpph Radical ◽

Tannin Content ◽

Dpph Radical Scavenging Activity ◽

Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

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Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

10.21203/rs.3.rs-930356/v1 ◽

2021 ◽

Author(s):

V. Renuka ◽

Chandragiri Nagaraja Rao Ravishankar ◽

Elavarasan Krishnamoorthy ◽

Abubacker Aliyamveetil Zynudheen

Keyword(s):

Thermal Behavior ◽

Antioxidant Activities ◽

Proteolytic Enzymes ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Structural Difference ◽

Dpph Radical ◽

Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

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Antioxidant activity and total phenolic and flavonoid contents of Salvia amplexicaulis Lam. extracts

Archives of Biological Sciences ◽

10.2298/abs1401307a ◽

2014 ◽

Vol 66 (1) ◽

pp. 307-316 ◽

Cited By ~ 9

Author(s):

Ana Alimpic ◽

Mariana Oaldje ◽

V. Matevski ◽

P.D. Marin ◽

Sonja Duletic-Lausevic

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanol Extract ◽

Total Phenolic ◽

Methanol Extracts ◽

Whole Plant ◽

Phenolic And Flavonoid

This study was designed to examine the in vitro antioxidant DPPH free radical-scavenging activity, and the total phenolic and flavonoid content of ethanol and methanol extracts of Salvia amplexicaulis Lam. in the whole plant and different parts, leaves, stems and flowers. The largest amounts of extract yield were obtained from the flowers, 14.14% and 12.00 % (w/w) in the ethanol of methanol extracts, respectively. The ethanol extract of leaves (16.07 ?g/ml) and methanol extract of the whole plant (21.28 ?g/ml) showed the highest activity against the DPPH radical. The ethanol extract of the leaves was the richest in phenols (222.40 mg GAE/g) and flavonoids (49.81 mg QE/g), whereas the methanol extract of the whole plant contained the highest amount of phenolics (180.89 mg GAE/g) and flavonoids (38.15 mg QE/g). A very strong linear correlation between antioxidant activity and the phenolic content of the extracts was established. The obtained results suggest that S. amplexicaulis could be regarded as an important source of natural antioxidants.

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In vitro Bioactivity of Essential Oils and Methanol Extracts of Salvia reuterana from Iran

Natural Product Communications ◽

10.1177/1934578x1200700527 ◽

2012 ◽

Vol 7 (5) ◽

pp. 1934578X1200700

Author(s):

Javad Safaei Ghomi ◽

Reihaneh Masoomi ◽

Fereshteh Jookar Kashi ◽

Hossein Batooli

Keyword(s):

Linoleic Acid ◽

Essential Oils ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Methanol Extracts ◽

Phenolic Contents ◽

Β Carotene

The chemical composition of the essential oils, antioxidant activity (DPPH and β-carotene/linoleic acid assays) and total phenolic content (Foline-Ciocalteu) of the flowers and leaves of Salvia reuterana were determined. Essential oils extracted from the flowers and leaves by hydrodistillation were analyzed by GC and GC/MS. Forty-four constituents, representing 99.7-99.9% of the oils, were identified. The major components were germacrene D, benzoic acid hexyl ester, bicyclogermacrene, β-gurjunene and ishwarene, constituting 33.7-31.9% of the oils. The highest radical-scavenging activity (DPPH test) was shown by the methanol extract of the flowers (IC50=77.6 μg/mL). In the β-carotene/linoleic acid assay, the methanol extract of the leaves showed the highest inhibition (40.3%) which was only slightly lower than that shown by BHT (82.9%). The total phenolic contents of the methanol extracts of the flowers and leaves as gallic acid equivalents were 81.4 and 88.3 μg/mg, respectively. The plant also showed good antimicrobial activity against three strains of tested microorganisms.

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