scholarly journals TMT Quantitative Proteomics Analysis Reveals the Effects of Transport Stress on Iron Metabolism in the Liver of Chicken

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 52
Author(s):  
Jun Liu ◽  
Dunhua Liu ◽  
Xun Wu ◽  
Cuili Pan ◽  
Shuzhe Wang ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Binding Capacity ◽  
Interaction Network ◽  
Proteome Analysis ◽  
Iron Level ◽  
Liver Iron ◽  
Serum Iron Level ◽  
Liver Iron Content ◽  
Transport Stress ◽  
Iron Binding Capacity

Abnormal iron metabolism can cause oxidative stress in broilers, and transport stress (TS) may potentially influence iron metabolism. However, the mechanisms by which TS affects iron metabolism are unclear. This study used quantitative proteome analysis based on tandem mass tag (TMT) to investigate the effects of TS on liver iron metabolism in broilers. Broilers (n = 24) reared under the same conditions were selected randomly into the transported group for 4 h (T2) and non-transported group (T1). Results showed that the serum iron level and total iron-binding capacity of broilers in the T2 were significantly higher than those in the T1 (p < 0.05). The liver iron content of broilers in the T2 (0.498 ± 0.058 mg·gprot−1) was significantly higher than that in the T1 (0.357 ± 0.035 mg·gprot−1), and the iron-stained sections showed that TS caused the enrichment of iron in the liver. We identified 1139 differentially expressed proteins (DEPs). Twelve DEPs associated with iron metabolism were identified, of which eight were up-regulated, and four were down-regulated in T2 compared with T1. Prediction of the protein interaction network for DEPs showed that FTH1, IREB2, and HEPH play vital roles in this network. The results provide new insights into the effects of TS on broilers’ liver iron metabolism.

scholarly journals Refractory Sideropenic Anemia in Childhood Due to an Error of Iron Metabolism

Blood ◽  
1955 ◽  
Vol 10 (12) ◽  
pp. 1246-1255 ◽  
Author(s):  
ROLF ZETTERSTRÖM ◽  
SIMONE DELAVA
Keyword(s):  
Iron Metabolism ◽  
Slow Rate ◽  
Alimentary Tract ◽  
Binding Capacity ◽  
Intravenous Iron ◽  
Iron Level ◽  
Serum Iron Level ◽  
Plummer Vinson Syndrome ◽  
Intravenous Iron Therapy ◽  
Iron Binding Capacity

Abstract Two cases of essential hypochronic anemia in childhood are reported. One of the cases which developed a mild Plummer-Vinson syndrome was completely refractory to peroral as well as intravenous iron therapy. In both cases the iron metabolism has been studied by means of radioiron. The following characteristics were found: The serum iron level was extremely low but the iron binding capacity was normal. The absorption of iron from the alimentary tract was defective and intravenously injected radioiron was utilized for hemoglobin synthesis at a slow rate. There was a rapid plasma iron turnover. The hypothesis is offered that the cause of the anemia resided in an abnormality in the regulation of iron metabolism.

Influence of hepcidin on iron homeostasis during last trimester of gestation in Bos indicus (cattle)

2019 ◽  
Author(s):  
K. Rajamanickam ◽  
M. Sameer Ali ◽  
V. Leela
Keyword(s):  
Serum Iron ◽  
Iron Homeostasis ◽  
Binding Capacity ◽  
Colorimetric Method ◽  
Maternal Serum ◽  
Iron Bioavailability ◽  
Iron Level ◽  
Iron Binding ◽  
Serum Iron Level ◽  
Iron Binding Capacity

Hepcidin is an important hormone regulating the systemic iron bio-availability. Blood samples were collected from thirty pregnant cattle during their last trimester of pregnancy to assess the relation of hepcidin to iron homeostasis. Serum hepcidin level was quantified using ELISA and serum iron, transferrin iron binding capacity and unsaturated iron binding capacity were estimated by colorimetric method. Correlation between hepcidin and other iron related parameters was assessed. Dependency of serum iron level on hepcidin was also determined by regression method. It was revealed that hepcidin was negatively correlated to serum iron and transferrin iron binding capacity (p less than 0.001) and also hepcidin has highest predictive value on serum iron level and transferrin iron binding capacity (p less than 0.001). It can be concluded that during pregnancy increase in hepcidin reduces the maternal serum iron and also it is a biomarker for iron bioavailability to the developing fetus.

scholarly journals The U-shaped association of serum iron level with COVID-19 severity: Is iron a potential therapeutic target?

2021 ◽  
Author(s):  
Kentaro Tojo ◽  
Yoh Sugawara ◽  
Yasufumi Oi ◽  
Fumihiro Ogawa ◽  
Takuma Higurashi ◽  
...  
Keyword(s):  
Disease Severity ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
University Hospital ◽  
Iron Level ◽  
Inadequate Response ◽  
Serum Iron Level ◽  
Median Serum ◽  
Iron Binding Capacity

AbstractObjectiveTo evaluate the association between iron metabolism indicators and disease severity in hospitalized patients with coronavirus disease 2019 (COVID-19).DesignTwo-center observational studySettingA university hospital and a core hospital in Yokohama, JapanPatientsAdults with COVID-19 whose serum iron levels were measured within the first 5 days of hospitalization were included. Patients who refused mechanical ventilation were excluded from the study.Measurements and Main ResultsOne hundred thirty-six patients were included in this study. We analyzed the association between COVID-19 severity and serum iron, total iron binding capacity (TIBC), and transferrin saturation (TSAT) levels. Disease severity was defined as the worst respiratory status during hospitalization. Serum iron levels were significantly lower in patients with mild respiratory failure (RF) (n=55, median serum iron level: 24 [interquartile range: 19–42] mg/dL) than in the non-RF group (n=44, 40 [24–80] mg/dL) and the severe RF group (n=37, 60 [23.5–87] mg/dL); however, there were no significant differences in iron levels between the non-RF and severe RF groups (non-RF vs. mild RF: p=0.019, non-RF vs. severe RF: p>0.999, and mild RF vs. severe RF: p=0.009). That is, there was a U-shaped association between serum iron levels and disease severity. TIBC levels decreased significantly with increasing severity; consequently, TSAT was significantly higher in patients with severe RF than in other patients. Multivariate analysis including only patients with RF adjusted for age and sex demonstrated that higher serum iron or TSAT levels were independently associated with development of severe RF.ConclusionsA U-shaped association between serum iron level and RF severity in hospitalized COVID-19 patients was observed. Higher serum iron levels in COVID-19 patients with RF are associated with the development of severe RF, indicating that inadequate response to lower serum iron might be an exacerbating factor for COVID-19.

Evaluation of serum iron and iron binding capacity in cows with paratuberculosis

2009 ◽  
Vol 37 (06) ◽  
pp. 375-378 ◽  
Author(s):  
Z. Metcitoglu ◽  
M. Ulgen ◽  
E. Borum ◽  
E. Temizel ◽  
S. Kasap ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Serum Iron ◽  
Binding Capacity ◽  
Control Group ◽  
Iron Level ◽  
Iron Binding ◽  
Serum Iron Level ◽  
Haematological Values ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Iron Binding Capacity

Summary Objective: The purpose of the study was to evaluate the results of routine haematological, serum iron and iron binding capacity analyses in cattle with Mycobacterium avium subsp. paratuberculosis (MAP) infection. Material and methods: Thirty-eight cows were included in the study. An ELISA for MAP, intradermal Johnin test and microscopic examination of the animals faecal smears for acid fast organisms were performed. According to the results 19 cows (4–8 years old) were found positive and 19 cows (4–7 years old) from the same herd were negative and served as control group. In all cows routine haematological values as well as serum iron level and iron binding capacity were evaluated. Results: When compared with control animals, routine haematological values including haematocrit, haemoglobin and erythrocyte counts were significantly (p < 0.05) lower in positive cows. Also serum iron level (p < 0.01) and iron binding capacity (p < 0.01) were significantly lower in positive cows than in the control group. Conclusion: As a result, these parameters can be important for the mechanism of the pathogenesis of paratuberculosis.

scholarly journals The U-shaped association of serum iron level with disease severity in adult hospitalized patients with COVID-19

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kentaro Tojo ◽  
Yoh Sugawara ◽  
Yasufumi Oi ◽  
Fumihiro Ogawa ◽  
Takuma Higurashi ◽  
...  
Keyword(s):  
Disease Severity ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Level ◽  
Inadequate Response ◽  
Lower Serum ◽  
Serum Iron Level ◽  
Iron Binding Capacity

AbstractCoronavirus disease 2019 (COVID-19) is an emerging infectious disease that leads to severe respiratory failure (RF). It is known that host exposure to viral infection triggers an iron-lowering response to mitigate pathogenic load and tissue damage. However, the association between host iron-lowering response and COVID-19 severity is not clear. This two-center observational study of 136 adult hospitalized COVID-19 patients analyzed the association between disease severity and initial serum iron, total iron-binding capacity (TIBC), and transferrin saturation (TSAT) levels. Serum iron levels were significantly lower in patients with mild RF than in the non-RF group; however, there were no significant differences in iron levels between the non-RF and severe RF groups, depicting a U-shaped association between serum iron levels and disease severity. TIBC levels decreased significantly with increasing severity; consequently, TSAT was significantly higher in patients with severe RF than in other patients. Multivariate analysis including only patients with RF adjusted for age and sex demonstrated that higher serum iron and TSAT levels were independently associated with the development of severe RF, indicating that inadequate response to lower serum iron might be an exacerbating factor for COVID-19.

scholarly journals Lymphocyte labile iron pool, plasma iron, transferrin saturation and ferritin levels in colon cancer patients.

2002 ◽  
Vol 49 (1) ◽  
pp. 269-272 ◽  
Author(s):  
Daniel Gackowski ◽  
Marcin Kruszewsk ◽  
Zbigniew Banaszkiewicz ◽  
Arkadiusz Jawien ◽  
Ryszard Olinski
Keyword(s):  
Binding Capacity ◽  
Transferrin Saturation ◽  
Control Group ◽  
Iron Level ◽  
Iron Availability ◽  
Serum Iron Level ◽  
Labile Iron Pool ◽  
Labile Iron ◽  
Iron Pool ◽  
Iron Binding Capacity

Patients with colorectal carcinoma showed statistically significant lower values of transferrin saturation, total iron binding capacity and serum iron level as compared with control group, while the level of ferritin and the size of labile iron pool in carcinoma patients were higher, although this difference was not statistically significant. Our observations are in favour of the hypothesis which suggests that changes in iron metabolism restrict iron availability for tumour cells and as consequence, slow their growth.

scholarly journals Iron deficiency anemia: current strategies for the diagnosis and management

2013 ◽  
Vol 4 (3) ◽  
pp. 193-202 ◽  
Author(s):  
Zühre Kaya
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Binding Capacity ◽  
Ferritin Level ◽  
Nutritional Deficiencies ◽  
Deficiency Anemia ◽  
Iron Level ◽  
Serum Iron Level ◽  
Diagnosis And Management ◽  
Low Serum

Iron deficiency is one of the commonest nutritional deficiencies in the world. It is multifactorial and may be caused by lack of intake, blood loss and intestinal causes. Clinical features are highly variable, and most patients are asymptomatic. Typical laboratory features of iron deficiency anemia (IDA) include a hypochromic microcytic anemia, low serum iron level, high total iron binding capacity, low serum ferritin level. Usefulness of monitoring serum transferrin receptor level (sTfR) and hepcidin for identifying IDA have been examined in a few studies. Available data suggest that sTfR can potentially become a valuable tool for regular testing of patients in the future. Despite IDA is easily corrected with iron therapy, establishing the cause can be difficult, particularly in cases caused by disorders of iron transport. Education for clinician needs to focus on increasing awareness of the importance of failure respond to iron supplementation. The aim of this review was to outline the current strategies for the diagnosis and management of IDA in the light of the latest reports.

scholarly journals STUDY OF SERUM FERRITIN IN SMOKERS

2018 ◽  
Vol 11 (1) ◽  
pp. 374
Author(s):  
Meera Shivasekar ◽  
Vinodhini Vm ◽  
Rupesh Kumar Y
Keyword(s):  
Cigarette Smoking ◽  
Serum Ferritin ◽  
Binding Capacity ◽  
Iron Status ◽  
Public Health Problem ◽  
Premature Death ◽  
Iron Level ◽  
Global Public Health ◽  
Serum Iron Level ◽  
Clinical Biomarkers

 Objective: Cigarette smoking is a major global public health problem and increases in the prevalence of tobacco smoking is the cause premature death worldwide. Serum ferritin an intracellular protein that can store and release iron is considered to be one of the important clinical biomarkers to evaluate iron status. This study explores the effect of cigarette smoking on serum ferritin level.Methods: The study was carried out in 100 cigarette smokers and 100 nonsmokers.Results: Subjects with smoking habits showed a significant increase in the serum ferritin levels compared to nonsmokers. Serum iron level, as well as total iron-binding capacity, showed significant increase compared with nonsmokers. Serum ferritin is found to correlate with serum iron.Conclusion: This study supports the fact that cigarette smoking has adverse effect on serum ferritin and other hematologic parameters, and serum ferritin is one of the most reliable indicators of iron status.

scholarly journals Hookworm Anemia: Iron Metabolism and Erythrokinetics

Blood ◽  
1961 ◽  
Vol 18 (1) ◽  
pp. 61-72 ◽  
Author(s):  
MIGUEL LAYRISSE ◽  
ALFREDO PAZ ◽  
NORMA BLUMENFELD ◽  
MARCEL ROCHE ◽  
Iris Dugarte ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Iron Absorption ◽  
Binding Capacity ◽  
Fecal Excretion ◽  
Red Cell ◽  
Hookworm Infection ◽  
Cell Production ◽  
Plasma Iron ◽  
Deficiency Type ◽  
Iron Binding Capacity

Abstract Iron metabolism, balance of red cell production and destruction and iron absorption from hemoglobin were determined in 11 patients with heavy hookworm infection and severe anemia. The plasma iron, total iron binding capacity, bone marrow hemosiderin and plasma Fe59 clearance are in agreement with the idea that the anemia associated with hookworm infection is of the iron deficiency type. The rate of red cell production measured by the E/M ratio, absolute reticulocyte count and plasma iron turnover showed an increase to about twice normal, while the rate of destruction estimated by the T ½ erythrocyte survival showed a destruction about 5 times normal. This unbalance between production and destruction could explain the severity of the anemia. The increase of fecal urobilinogen output to twice normal was interpreted as due to the metabolism of the hemoglobin lost into the intestine rather than to an increase of hemolysis. The estimation of fecal blood loss in the patients whose red cells were tagged with Cr51 and Fe59, showed that the radioactivity counted with Fe59 was only about 63 per cent of the radioactivity counted with Cr51. This difference was interpreted as due to iron absorption from the hemoglobin lost into the intestine. The mean daily fecal excretion of iron reaches 4.7 mg. Since the iron metabolism in these patients is in equilibrium, we have concluded that the iron loss is replaced by the iron from food; this is in addition to the 3 mg. hemoglobin iron which is reabsorbed from the blood lost into the gut.

scholarly journals Clinical study of anemia in women attending the College of Medical Sciences Bharatpur

2015 ◽  
Vol 10 (3) ◽  
pp. 23-28
Author(s):  
M Pradhan
Keyword(s):  
Teaching Hospital ◽  
Binding Capacity ◽  
Clinical Signs ◽  
Hypochromic Anemia ◽  
Signs And Symptoms ◽  
Dietary Advice ◽  
Iron Level ◽  
Medical Sciences ◽  
Serum Iron Level ◽  
Nutritional Anemia

OBJECTIVE To study the clinical profile of Anemia in Women attending the College of Medical Sciences-Teaching Hospital, Bharatpur, Chitwan.MATERIALS & METHODS The Study was conducted among 100 women patients attended to the College of Medical Sciences- Teaching Hospital, Bharatpur, Chitwan. Their blood samples were tested and related socio-demographic information was collected. The associated Clinical signs and symptoms with hemoglobin level and the occurrence of anemia were assessed.RESULT The peak incidence of Anemia was in the age group of 20-24 yrs (59.7%), and severity of anemia was correlated with sings and symptoms. Majority of them had Microcytic Hypochromic anemia (43.1%) with serum iron level below normal level and increased TIBC (total iron binding capacity) were observed.CONCLUSION The high prevalence of Nutritional anemia suggests the dietary advice to be emphasized strongly.Journal of College of Medical Sciences-Nepal, 2014, Vol-10, No-3, 23-28

Sign in / Sign up

Export Citation Format

Share Document