scholarly journals Characterization of Circular RNAs in Chinese Buffalo (Bubalus bubalis) Adipose Tissue: A Focus on Circular RNAs Involved in Fat Deposition

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 403 ◽  
Author(s):  
Jieping Huang ◽  
Jinhui Zhao ◽  
Qiuzhi Zheng ◽  
Shuzhe Wang ◽  
Xuefeng Wei ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Strong Correlation ◽  
Bubalus Bubalis ◽  
Fat Deposition ◽  
Circular Rnas ◽  
Strongly Correlated ◽  
Illumina Hiseq ◽  
Qrt Pcr ◽  
First Time

Circular RNAs (circRNAs) have been identified as a novel type of regulators involved in multiple biological processes. However, circRNAs with a potential function in fat deposition in buffalo are poorly understood. In this study, six RNA libraries of adipose tissue were constructed for three young and three adult Chinese buffaloes with paired-ends RNA sequencing using the Illumina HiSeq 3000 platform. A total of 5141 circRNAs were computationally identified. Among them, 252 circRNAs were differentially expressed (DE) between the young and adult buffaloes. Of these, 54 were upregulated and 198 were downregulated in the adult group. Eight DE circRNAs were further identified by quantitative real-time-PCR (qRT-PCR) and Sanger sequencing. Co-expression analysis revealed that 34 circRNAs demonstrated a strong correlation with fat deposition-associated genes (|r| > 0.980). Among these, expressional correlation between two circRNAs (19:45387150|45389986 and 21:6969877|69753491) and PR/SET domain 16 was further verified using qRT-PCR, and a strong correlation was revealed (1 > |r| > 0.8). These results strongly suggest that circRNAs 19:45387150|45389986 and 21:6969877|69753491 are potential regulators of buffalo fat deposition. In summary, this study characterized the circRNA profiles of adipose tissues at different stages for the first time and revealed two circRNAs strongly correlated with fat deposition-associated genes, which provided new candidate regulators for fat deposition in buffalo.

scholarly journals Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

2020 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results : A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions : Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan.

2017 ◽  
Author(s):  
Syed Khurram Fareed ◽  
Faiz Muhammad ◽  
Javed Muhammad ◽  
Masood Rabbani ◽  
Taseer Ahmed Khan ◽  
...  
Keyword(s):  
Respiratory Distress ◽  
Bubalus Bubalis ◽  
Nasal Discharge ◽  
Specific Primers ◽  
Tissue Samples ◽  
Acholeplasma Laidlawii ◽  
Precautionary Measures ◽  
First Time ◽  
Culture Positive

The present study was conducted to identify and characterize the bovine Acholeplasma laidlawii from 1120 clinically ill buffaloes and 470 lungs collected from buffaloes at abattoir of Karachi. All buffaloes were clinically examined and around 467 (13.48%) buffaloes had signs of respiratory distress including sticky nasal discharge, sunken eyes, dyspnea and fever. All the culture positive samples were further evaluated by PCR using specific primers of A. laidlawii and the amplified product to re-validate the sequence of the specie. This revealed that suspected buffaloes, 92 (2.66%) from nasal discharge and 44 (4.03%) from lung tissue samples respectively were positive for A. laidlawii. Rests of the samples were still under investigation. However it is first time proclaimed on research basis that A. laidlawii was infecting the buffaloes in Pakistan and precautionary measures should be taken to prevent losses from this specie in the months of October and April.

scholarly journals Canine colostrum exosomes: characterization and influence on canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

2020 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland fundamental for the survival of the newborn. It has many described components (immunoglobulins, proteins or fat), but small vesicles named exosomes are largely unknown. Results: A characterization of the CCM exosomes has been performed. Exosome concentrations were abundant in CCM and appeared with characteristic cup-shaped morphology and well-defined round vesicles. Their size distribution was between 37−140 nm and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosomes cargo. We also found that exosomes modified proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Besides, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions: Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to the two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed a higher potential in adipose tissue development and neurogenesis, while cBM-MSCs production was associated with immunity, cell mobilization and hematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating a fundamental role in the development and maturation of the puppy in the early stages of its life.

scholarly journals Surface Reflectance: An Optical Method for Multiscale Curvature Characterization of Wear on Ceramic–Metal Composites

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1024 ◽  
Author(s):  
Julie Lemesle ◽  
Frederic Robache ◽  
Gaetan Le Goic ◽  
Alamin Mansouri ◽  
Christopher A. Brown ◽  
...  
Keyword(s):  
Strong Correlation ◽  
Band Pass Filter ◽  
Metal Composites ◽  
Pass Filter ◽  
Surface Gradient ◽  
Light Reflectance ◽  
Band Pass ◽  
First Time ◽  
High Pass

Surface gradient characterization by light reflectance (SGCLR) is used for the first time for multiscale curvature calculations and discrimination of worn surfaces on six damaged ceramic–metal composites. Measurements are made using reflectance transformation imaging (RTI). Slope and curvature maps, generated from RTI, are analyzed instead of heights. From multiscale decompositions, bootstrapping, and analysis of variance (ANOVA), a strong correlation (R² = 0.90) is found between the density of furrows of Mehlum curvatures, with a band pass filter at 5.4 µm, present in ceramic grains and their mechanical properties. A strong correlation is found between the mean curvatures of the metal and the ceramics, with a high pass filter at 1286 µm.

scholarly journals Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

2020 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results : A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions : Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

scholarly journals Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

2020 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown.Results: A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture.Conclusions: Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

scholarly journals Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Antonio J. Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

scholarly journals A Novel Circular RNA hsa_circ_20403 Predicts Poor Prognosis and Promotes Progression in Osteosarcoma

2020 ◽  
Author(s):  
Rui Li ◽  
Renzan Zhang ◽  
Zhengpeng Liu ◽  
Yilong Zhang
Keyword(s):  
Biological Function ◽  
Bone Cancer ◽  
Circular Rna ◽  
Migration And Invasion ◽  
Circular Rnas ◽  
Illumina Hiseq ◽  
Promote Cell Proliferation ◽  
Cancer Initiation ◽  
Tissue Specimens ◽  
First Time

Abstract Objective Osteosarcoma (OS) is the most frequent bone cancer in humans. Growing evidence suggests that circular RNAs (circRNAs) exert essential roles in regulating cancer initiation and metastasis. Some research were used Illumina HiSeq to screens differentially expressed of circRNAs in OS and found that hsa_circ_20403 is highly expressed in OS. Methods In this study, we investigated the clinical application value and biological function of hsa_circ_20403 in OS. We were performed to verified the biological functions by CCK-8, flow cytometry, and transwell experiments. Results We found that the expression of circ_20403 is enhanced in both OS tissue specimens and cells. In addition, its expression is closely related to clinic opathological characteristics and prognosis. More importantly, circ_20403 was overexpressed and knocked down in OS cells. We found that circ_20403 can promote cell proliferation, migration and invasion, which can regulate the proportion of G1 and S phase cells. Conclusion Taken together, our study for the first time revealed hsa_circ_20403 as a carcinogen to promote the progression of OS. Our study suggested that hsa_circ_20403 can be used to predict the prognosis of OS and it might be a potential target for OS therapy.

scholarly journals Characterization of GPX1 and DIO1 Genes in Bubalus Bubalis

2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Vandana Dureja ◽  
Sumer Singh ◽  
S Rana ◽  
P Banerjee ◽  
J Joshi ◽  
...  
Keyword(s):  
Active Form ◽  
Bubalus Bubalis ◽  
Main Function ◽  
Exon 2 ◽  
Iodothyronine Deiodinases ◽  
Exon 1 ◽  
Selenoprotein Genes ◽  
Living Organisms ◽  
First Time

Selenoprotein genes contain selenium in the form of selenocysteine which is involved in protecting the cells from oxidative stress. Soils in India differ greatly in selenium concentrations affecting feed stuffs for selenium availability. Selenoproteins have recently been identified in variety of living organisms including humans which have 25 selenoprotein genes. Among these families of selenoprotein genes, we sequenced Gpx1 gene (Glutathione peroxidases1) and Dio1 gene (Iodothyronine deiodinases) in Bubalus bubalis. Gpx1 is most abundant and ubiquitously expressed selenoprotein which helps to protect against the damaging effects of hydrogen peroxide and oxygen rich free radicals whereas Dio1 is expressed mainly in liver, thyroid gland and adipose tissue, its main function is to convert tetraiodothyronine (T4)  to its active form thyroxine (T3) in the presence of deiodinases enzyme. The main aim of the study was to characterize these two genes and to find out the buffalo specific SNPs. This was accomplished by designing primers using cattle database and sequencing a panel of 24 samples consisting of 6 diverse breeds of buffalo. Gpx1 consisted of 2 exons (Accession ID: JQ031269) while Dio1 comprised 4 exons (Accession ID: JQ791197). In Gpx1 gene, 9 SNPs were recorded and 4 were non synonymous, changing amino acid were distributed equally in both exon. In exon 1, A141G (aa Q5R) and G161A (aa A12T); and in exon 2 C785T (aa R132W) and A808T (aa S139R). In Dio1 gene, 3 non synonymous SNPs were identified at A188G (aa H22R), C215G (aa T31R) and G941A (aa V146I). These SNPs are novel and reported for the first time in Indian buffalo and has a potential for their use in diversity analysis and association with various selenium related traits.

scholarly journals In VitroandIn VivoEffects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Agnieszka Śmieszek ◽  
Katarzyna Basińska ◽  
Klaudia Chrząstek ◽  
Krzysztof Marycz
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Proliferative Potential ◽  
Dependent Manner ◽  
Strongly Correlated ◽  
Treatment Of Obesity ◽  
First Time ◽  
Lower Expression

Metformin is applied not only as antidiabetic drug, but also in the treatment of obesity or as antiaging drug. The first part of the research discussed the effect of metformin at concentrations of 1 mM, 5 mM, and 10 mM on the morphology, ultrastructure, and proliferation potential of mice adipose-derived multipotent mesenchymal stromal cells (ASCs)in vitro. Additionally, we determined the influence of metformin on mice adipose tissue metabolism. This study has shown for the first time that metformin inhibits the proliferative potential of ASCsin vitroin a dose- and time-dependent manner. In addition, we have found a significant correlation between the activity of ASCs and osteopontin at the mRNA and protein level. Furthermore, we have demonstrated that 5 mM and 10 mM metformin have cytotoxic effect on ASCs, causing severe morphological, ultrastructural, and apoptotic changes. The reduced level of OPN in the adipose tissue of metformin-treated animals strongly correlated with the lower expression of Ki67 and CD105 and increased caspase-3. The metformin influenced also circulating levels of OPN, which is what was found with systemic and local action of metformin. The results are a valuable source of information regarding thein vitroeffect of metformin on adipose-derived stem cells.

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