Effects of Vitamin E and Coenzyme Q10 Supplementation on Oxidative Stress Parameters in Untrained Leisure Horses Subjected to Acute Moderate Exercise

The effects of antioxidant supplements on exercise-induced oxidative stress have not been investigated in untrained leisure horses. We investigated the effects of 14-day supplementation with vitamin E (1.8 IU/kg/day), coenzyme Q10 (CoQ10; ubiquinone; 800 mg/day), and a combination of both (the same doses as in mono-supplementation) on the blood levels of CoQ10, vitamin E, and oxidative stress parameters in untrained leisure horses subjected to acute moderate exercise. Correlations between lipid peroxidation and muscle enzyme leakage were also determined. Forty client-owned horses were included in the study, with 10 horses in each of the antioxidant and placebo (paraffin oil) groups. Blood parameters were measured before supplementation, before and immediately after exercise, and after 24 h of rest. The differences in individual parameters between blood collection times and groups were analysed with linear mixed models (p ˂ 0.05). None of the supplemented antioxidants affected vitamin E and CoQ10 concentrations, oxidative stress parameters, or serum muscle enzymes. Lipid peroxidation occurred in horses supplemented with placebo and CoQ10 but not in horses supplemented with vitamin E or the combination of both antioxidants. These results suggest that vitamin E alone or in combination with CoQ10 prevented lipid peroxidation in untrained leisure horses subjected to acute moderate exercise.

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Comparing the effects of Portulaca oleracea seed hydro-alcoholic extract, valsartan, and vitamin E on hemodynamic changes, oxidative stress parameters and cardiac hypertrophy in thyrotoxic rats

Drug and Chemical Toxicology ◽

10.1080/01480545.2019.1651330 ◽

2019 ◽

pp. 1-8

Author(s):

Roghayeh Pakdel ◽

Mehran Vatanchian ◽

Saeed Niazmand ◽

Farimah Beheshti ◽

Maryam Rahimi ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Cardiac Hypertrophy ◽

Portulaca Oleracea ◽

Alcoholic Extract ◽

Hemodynamic Changes ◽

Oxidative Stress Parameters ◽

Stress Parameters

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Changes in Oxidative Stress Parameters as Response to Supplementation With Vitamin E and Omega-3 Fatty Acids in Male Workers Exposed to High Level Noise

10.21203/rs.3.rs-850400/v1 ◽

2021 ◽

Author(s):

Hamzeh Mohammadi ◽

Farideh Golbabaei ◽

Somayeh Farhang Dehghan ◽

Nariman Moradi ◽

Maryam Chamari ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Vitamin E ◽

Assay Method ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Decremental Effect ◽

Oxidative Stress Parameters ◽

Incremental Effect ◽

Stress Parameters

Abstract Background: The present study aimed to assess the effects of the supplementation consumption of vitamin E and Omega 3 fatty acid on the oxidative stress parameters among workers exposed to high levels of noise in an automobile parts manufacturing plant. Moreover, the effects of the exposure to noise on SOD, total oxidative stress (TAC) and MDA will be investigated. Methods: The participants were deployed into 4 groups (vitamin E (100 mg), Omega 3 fatty acids (180 mg EPA and 120 mg DHA), vitamin E+ Omega 3 and Placebo), as per the double-blind block randomization method. The oxidative stress parameters of the participants were analyzed before and after three month consumption of supplements via enzyme-linked immunosorbent assay method. The level of workers' exposure to noise, was measured according to ISO 9612. Results: Before intervention, mean MDA, SOD and TAC were 27.52 (7.46) nmol/ml, 58.84 (10.44) U/ml and 2.57 (0.67) mM respectively. After intervention, mean MDA, SOD and TAC were 24.57 (7.58) nmol/ml, 63.46 (11.02) U/ml and 2.70 (0.84) mM respectively. The use of supplement Omega 3 fatty acids had a significant decremental effect on MDA levels. The use of vitamin E alongside Omega 3 fatty acids had a significant incremental effect on SOD activity.Conclusion: Noise exposure had a decremental effect on TAC and SOD as well as an incremental effect on MDA, but this was only statistically significant for TAC. It appears that the simultaneous use of vitamin E and Omega 3 fatty acids for three months had a positive effect on the anti-oxidant performance of workers exposed to noise.

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Protective Effects of Propolis and Probiotic Lactobacillus acidophilus against Carbon Tetrachloride-Induced Hepatotoxicity in Rats

Pharmaceutical Sciences ◽

10.15171/ps.2019.36 ◽

2019 ◽

Vol 25 (3) ◽

pp. 190-197 ◽

Cited By ~ 1

Author(s):

Abdelkader Oumeddour ◽

Djahida Zaroure ◽

Raziqua Haroune ◽

Rima Zaimeche ◽

Karima Riane ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Carbon Tetrachloride ◽

Total Protein ◽

Serum Biochemistry ◽

Protective Effects ◽

Oxidative Stress Parameters ◽

Therapeutic Benefits ◽

Sugar Levels ◽

Stress Parameters

Background: Propolis (PRS) and probiotic bacteria Lactobacillus are natural products used as dietary supplement for their therapeutic benefits. This study was performed to examine the possible hepatoprotective effect of PRS and probiotics (PRCs) against carbon tetrachloride-induced liver injury. Methods: Experimentally, intoxicated rats received 0.5 ml/kg CCl4 (i.p.) daily for six days, pretreated rats received per os PRS 100 mg/kg or PRCs 109 CFU for six days followed by a single dose of 0.5 ml/kg CCl4. Control groups received either PRS, PRCs or olive oil for six days. Then, serum biochemistry (total protein, cholesterol, triglycerides and albumin) and oxidative stress parameters were measured. Results: We showed that CCl4 treatment was associated with an increase of the serum aspartate amino transferase (AST), alanine aminotransferase (ALT), cholesterol and triglycerides levels. In parallel, serum total protein, albumin and blood sugar levels were significantly decreased. Regarding the oxidative stress parameters, catalase and glutathione S-transferase (GST) levels were lower, conversely to the lipid peroxidation (MDA). Conclusion: Our results strongly support that administration of PRS and PRCs may significantly protect liver against CCl4-induced toxicity by enhancing antioxidative stress pathway and preventing lipid peroxidation.

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Is the Clinical Course of Non-Arteritic Ischemic Optic Neuropathy Associated with Oxidative Damage and the Dynamics of the Antioxidant Response?

10.21203/rs.3.rs-1145784/v1 ◽

2022 ◽

Author(s):

kaya N engin ◽

Duygu Harmancı Karagülle ◽

Ceren Durmaz Engin ◽

Melis Kant ◽

Aylin Yaman ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Oxidative Damage ◽

Patient Group ◽

Optic Neuropathy ◽

Clinical Course ◽

Antioxidant Response ◽

Ischemic Optic Neuropathy ◽

Oxidative Stress Parameters ◽

Stress Parameters

Abstract Purpose: Oxidative stress is known to be a decisive factor in the wide etiopathogenesis of optic neuropathy. This study aimed to comprehensively evaluate the interaction of optic neuropathy’s clinical course with systemic oxidative damage and antioxidant response dynamics in a large series.Methods: This case-controlled clinical study included 33 non-arteritic anterior ischemic optic neuropathy patients and 32 healthy individuals. Extensive systemic oxidation profiles were statistically compared between the two groups, and correlations between the clinical and biochemical data in the patient group were analyzed.Results: Vitamin E and MDA levels were significantly higher in the patient group. Significant correlations were observed in the analyses between clinical findings and oxidative stress parameters. Correlations between vitamin E and intraocular pressure, between B12 and cup-to-disc ratio, between antioxidant glutathione and SOD enzyme systems, and between uric acid and age were found to be very significant. As significant correlations were found in either clinical and biochemical data or in oxidative stress parameters, correlations between vitamin E and cholesterol, MDA was found to be very significant. Conclusions: This study not only supplies significant information regarding oxidative damage and antioxidant response in NAION, but also points out the specific interactions of neuromodulators, like vitamin E, in intracellular signaling pathways and regulation mechanisms. A better reading of these connections may help improve diagnosis, follow-ups and treatment criteria and strategies.

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Effect of toluene on erythrocyte membrane stability under in vivo and in vitro conditions with assessment of oxidant/antioxidant status

Toxicology and Industrial Health ◽

10.1177/0748233709346758 ◽

2009 ◽

Vol 25 (8) ◽

pp. 545-550 ◽

Cited By ~ 15

Author(s):

Ismail Karabulut ◽

Z. Dicle Balkanci ◽

Bilge Pehlivanoglu ◽

Aysen Erdem ◽

Ersin Fadillioglu

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Unsaturated Fatty Acids ◽

Osmotic Fragility ◽

Human Erythrocytes ◽

Antioxidant Enzyme Activities ◽

Oxidative Stress Parameters ◽

Stress Parameters

Toluene, an organic solvent used widely in the industry, is highly lipophilic and accumulates in the cell membrane impeding transport through it. Its metabolites cause oxygen radical formation that react with unsaturated fatty acids and proteins in erythrocytes leading to lipid peroxidation and protein breakdown. In this study, we aimed to investigate the membrane stabilizing and the oxidative stress—inducing effects of toluene in human erythrocytes. Measurements of osmotic fragility, mean corpuscular volume (MCV), oxidative stress parameters and antioxidant enzyme activities were performed simultaneously both in individuals exposed to toluene professionally (in vivo) and human erythrocytes treated with toluene (in vitro). To measure osmotic fragility, erythrocytes were placed in NaCl solutions at various concentrations (0.1% [blank], 0.38%, 0.40%, 0.42%, 0.44%, 0.46%, 0.48% and 1% [stock]). Percentage of haemolysis in each solution was calculated with respect to the 100% haemolysis in the blank solution. The erythrocyte packs prepared at the day of the above-mentioned measurements were kept at —80°C until the time for determination of malonyldialdehyde and protein carbonyl levels, and catalase (CAT) and glutathione peroxidase activities as indicators of oxidative stress. Toluene increased oxidative stress parameters significantly both in vivo and in vitro; it also caused a significant decrease in the activities of antioxidant enzymes. Osmotic fragility was altered only in the case of in vitro exposure. In conclusion, toluene exposure resulted in increased lipid peroxidation and protein damage both in vivo and in vitro. Although, it is natural to expect increased osmotic fragility due to oxidative properties of toluene, its membrane-stabilizing effect overcame the oxidative properties leading to decreased osmotic fragility or preventing its deterioration in vitro and in vivo toluene exposures, respectively, in the present study.

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Effects of vitamin E supplementation and training on oxidative stress parameters measured in exercising horses

Comparative Exercise Physiology ◽

10.1017/s1478061509341353 ◽

2009 ◽

Vol 6 (01) ◽

pp. 17 ◽

Cited By ~ 4

Author(s):

K J Duberstein ◽

S E Johnson ◽

L R McDowell ◽

E A Ott

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Oxidative Stress Parameters ◽

And Training ◽

Stress Parameters ◽

Vitamin E Supplementation

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EFFECT OF TOCOTRIENOL PRETREATMENT ON EX VIVO SUPEROXIDE AND PEROXIDE HANDLING CAPACITIES (SPHC) OF RAT SERUM AND BRAIN

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i3.15866 ◽

2017 ◽

Vol 9 (3) ◽

pp. 116

Author(s):

Pitchaiah Dasari ◽

Anandamurali R. ◽

Prasunpriya Nayak

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Ex Vivo ◽

Reduced Glutathione ◽

Temporal Cortex ◽

Antioxidant Property ◽

Brain Regions ◽

Rat Serum ◽

Oxidative Stress Parameters ◽

Stress Parameters

Objective: Tocotrienol (TT), a constituent of vitamin E, present only in selected seed oil. Because of the isoprenoid side chain, antioxidant property of tocotrienol is recently highlighted. Application of tocotrienol is also proven to be neuroprotective. The current study was aimed to evaluate the effect of tocotrienol pretreatment on the serum and brain oxidative stress parameters and oxidant handling capacities.Methods: Male albino Wistar rats were treated with tocotrienol (10 mg/day) for two weeks and maintained for the next four weeks. Levels of reduced glutathione and lipid peroxidation and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were estimated fortnightly in serum. After sacrifice, oxidative stress parameters were measured in the frontal cortex, temporal cortex, thalamic area, hippocampus and cerebellum. Glutathione-dependent and glutathione-independent superoxide and peroxide handling capacities (SPHC) were calculated for serum and brain regions. Data collected from both the groups are statistically processed with Kruskal-Wallis test and Mann-Whitney pairwise comparisons.Results: Significant impacts of TT treatment have been observed in terms of growth and water intake. Serum SPHC (Glutathione-independent) has been found to be reduced significantly immediately after the TT treatment. Region-specific alterations in oxidative stress parameters have also been observed after 4 w of supplementation. Global reductions in reduced glutathione and lipid peroxidation have been observed in the brain without any alteration in the SPHC.Conclusion: From the results, it can be suggested that the tocotrienol pretreatment possibly be used as neuroprotective measure particularly against oxidative stress. In addition, the antioxidant impacts of TT were found to be maintained for a longer period in brain regions, even though it was not so in the case of serum.

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