scholarly journals Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1230
Author(s):  
Catherine Lizama ◽  
Javier Romero-Parra ◽  
Daniel Andrade ◽  
Felipe Riveros ◽  
Jorge Bórquez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Saline Lakes ◽  
High Resolution Mass Spectrometry ◽  
Cholinesterase Inhibition ◽  
Carotenoid Pigment ◽  
Skin Cell ◽  
Orbitrap Mass Spectrometry ◽  
Pigment Profile ◽  
First Time

Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC50 was 2.96 ± 0.08 mg/mL and BuChE inhibition IC50 was 2.39 ± 0.09 mg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers.

LOPIT-DC: A simpler approach to high-resolution spatial proteomics

2018 ◽  
Author(s):  
Aikaterini Geladaki ◽  
Nina Kočevar Britovšek ◽  
Lisa M. Breckels ◽  
Tom S. Smith ◽  
Claire M. Mulvey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Protein Complexes ◽  
Subcellular Localisation ◽  
Proteomics Data ◽  
Large Protein ◽  
Subcellular Resolution ◽  
First Time ◽  
Human Dataset

AbstractHyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method for studying protein subcellular localisation in complex biological samples. As a simpler alternative we developed a second workflow named Localisation of Organelle Proteins by Isotope Tagging after Differential ultraCentrifugation (LOPIT-DC) which is faster and less resource-intensive. We present the most comprehensive high-resolution mass spectrometry-based human dataset to date and deliver a flexible set of subcellular proteomics protocols for sample preparation and data analysis. For the first time, we methodically compare these two different mass spectrometry-based spatial proteomics methods within the same study and also apply QSep, the first tool that objectively and robustly quantifies subcellular resolution in spatial proteomics data. Using both approaches we highlight suborganellar resolution and isoform-specific subcellular niches as well as the locations of large protein complexes and proteins involved in signalling pathways which play important roles in cancer and metabolism. Finally, we showcase an extensive analysis of the multilocalising proteome identified via both methods.

scholarly journals Alkaloids from Papaver coreanum

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601
Author(s):  
Dong-Ung Lee ◽  
Jong Hee Park ◽  
Ludger Wessjohann ◽  
Jürgen Schmidt
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Endemic Plant ◽  
High Resolution Mass ◽  
Main Components ◽  
First Time ◽  
Resolution Mass

The alkaloid pattern of the endemic plant Papaver coreanum Nakai (Papaveraceae) was determined for the first time. Eight alkaloids could be identified by LC/ESIMS/MS and high-resolution mass spectrometry. Among them, protopine and allocryptopine represent the main components. Besides norsanguinarine, sanguinarine, dihydrosanguinarine, oxysanguinarine, lincangenine, and cryptopine, some other trace alkaloids were found whose structures remain unknown.

scholarly journals Chasing the Major Sphingolipids on Earth: Automated Annotation of Plant Glycosyl Inositol Phospho Ceramides by Glycolipidomics

Metabolites ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 375 ◽  
Author(s):  
Lisa Panzenboeck ◽  
Nina Troppmair ◽  
Sara Schlachter ◽  
Gunda Koellensperger ◽  
Jürgen Hartler ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Decision Rules ◽  
Reversed Phase ◽  
Plant Sample ◽  
Automated Annotation ◽  
Plant Samples ◽  
First Time

Glycosyl inositol phospho ceramides (GIPCs) are the major sphingolipids on earth, as they account for a considerable fraction of the total lipids in plants and fungi, which in turn represent a large portion of the biomass on earth. Despite their obvious importance, GIPC analysis remains challenging due to the lack of commercial standards and automated annotation software. In this work, we introduce a novel GIPC glycolipidomics workflow based on reversed-phase ultra-high pressure liquid chromatography coupled to high-resolution mass spectrometry. For the first time, automated GIPC assignment was performed using the open-source software Lipid Data Analyzer (LDA), based on platform-independent decision rules. Four different plant samples (salad, spinach, raspberry, and strawberry) were analyzed and the results revealed 64 GIPCs based on accurate mass, characteristic MS2 fragments and matching retention times. Relative quantification using lactosyl ceramide for internal standardization revealed GIPC t18:1/h24:0 as the most abundant species in all plants. Depending on the plant sample, GIPCs contained mainly amine, N-acetylamine or hydroxyl residues. Most GIPCs revealed a Hex-HexA-IPC core and contained a ceramide part with a trihydroxylated t18:0 or a t18:1 long chain base and hydroxylated fatty acid chains ranging from 16 to 26 carbon atoms in length (h16:0–h26:0). Interestingly, four GIPCs containing t18:2 were observed in the raspberry sample, which was not reported so far. The presented workflow supports the characterization of different plant samples by automatic GIPC assignment, potentially leading to the identification of new GIPCs. For the first time, automated high-throughput profiling of these complex glycolipids is possible by liquid chromatography-high-resolution tandem mass spectrometry and subsequent automated glycolipid annotation based on decision rules.

scholarly journals Taurine is Covalently Incorporated into Alpha Tubulin

2019 ◽  
Author(s):  
Matthew T Olson ◽  
Alfred L Yergey ◽  
Kamalika Mukherjee ◽  
melissa Pergande ◽  
susan Bane ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Free Amino ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Carboxyl Terminus ◽  
Alpha Tubulin ◽  
Avian Erythrocytes ◽  
First Time ◽  
Resolution Mass

We use various methods of high resolution mass spectrometry to demonstrate that taurine is covalently attached to the carboxyl terminus of alpha Tubulin from avian erythrocytes. The alpha Tubulin terminal residue, a tyrosine, must already be removed before covalent addition of taurine, via a known cycle of de-tyrosination and re-tyrosination. Taurination appears to stop this cycle. Taurine is the most abundant free amino acid in the body but this is the first time that it has been shown to be covalently incorporated into any protein.

scholarly journals Taurine is Covalently Incorporated into Alpha Tubulin

2019 ◽  
Author(s):  
Matthew T Olson ◽  
Alfred L Yergey ◽  
Kamalika Mukherjee ◽  
melissa Pergande ◽  
susan Bane ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Free Amino ◽  
High Resolution Mass Spectrometry ◽  
The Body ◽  
Carboxyl Terminus ◽  
Alpha Tubulin ◽  
Avian Erythrocytes ◽  
First Time ◽  
Resolution Mass

We use various methods of high resolution mass spectrometry to demonstrate that taurine is covalently attached to the carboxyl terminus of alpha Tubulin from avian erythrocytes. The alpha Tubulin terminal residue, a tyrosine, must already be removed before covalent addition of taurine, via a known cycle of de-tyrosination and re-tyrosination. Taurination appears to stop this cycle. Taurine is the most abundant free amino acid in the body but this is the first time that it has been shown to be covalently incorporated into any protein.

scholarly journals Hypochlorite-induced damage of plasminogen molecules: structural-functional disturbance

2019 ◽  
Vol 488 (5) ◽  
pp. 560-566
Author(s):  
A. D. Vasilyeva ◽  
L. V. Yurina ◽  
A. N. Shchegolikhin ◽  
A. E. Bugrova ◽  
T. S. Konstantinova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Structure ◽  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Data ◽  
High Tolerance ◽  
Functional Disturbance ◽  
Closed Conformation ◽  
First Time ◽  
Resolution Mass

Plasminogen being a precursor of plasmin, a serine protease which plays a fundamental role in the intravascular thrombolysis. For the first time, by using high-resolution mass spectrometry, data were obtained of oxidative modifications of the plasminogen molecule under induced oxidation. The FTIR data show that under oxidation on the protein, its secondary structure also undergoes the rearrangements. The high tolerance of plasminogen to oxidation can be due to both the closed conformation and the ability of some Met residues to serve as ROS trap.

scholarly journals New Phenolic Compounds in Posidonia oceanica Seagrass: A Comprehensive Array Using High Resolution Mass Spectrometry

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 864
Author(s):  
Marina Astudillo-Pascual ◽  
Irene Domínguez ◽  
Pedro A. Aguilera ◽  
Antonia Garrido Frenich
Keyword(s):  
Mass Spectrometry ◽  
Phenolic Compounds ◽  
High Resolution ◽  
Detection Method ◽  
High Resolution Mass Spectrometry ◽  
Posidonia Oceanica ◽  
High Resolution Mass ◽  
First Time ◽  
Different Tissues ◽  
Resolution Mass

The studies on the Posidonia oceanica Delile (P. oceanica) phenolic composition have been focused on the foliar tissues and have often neglected the phenolic compounds in rhizomes or roots alike. With the current improvements in high resolution mass spectrometry (HRMS) analyzers, such as the Orbitrap MS, there is a new opportunity to more deeply study P. oceanica. One of the benefits is the possibility of conducting an exhaustive phenolic monitoring, which is crucial in the search for new stressor-specific biomarkers of coastal deterioration. For this purpose, the different tissues (leaf, rhizome, and root) of P. oceanica seagrass from several marine sampling areas were analyzed through target, suspected, and non-target screenings. This paper brings a fast and tissues-specific extraction, as well as a detection method of phenolic compounds applying for the first time the potential of HRMS (Exactive Orbitrap) in P. oceanica samples. As a result, 42 phenolic compounds were satisfactorily detected, of which, to our knowledge, 24 were not previously reported in P. oceanica, such as naringenin, naringenin chalcone and pinocembrin, among others. Information here reported could be used for the evaluation of new stressor-specific biomarkers of coastal deterioration in the Mediterranean waters. Furthermore, the followed extraction and analytical method could be considered as a reference protocol in other studies on marine seagrasses due to the exhaustive search and satisfactory results.

Investigation of the archeological remains using ultrahigh resolution mass spectrometry

2019 ◽  
Vol 25 (4) ◽  
pp. 391-396 ◽  
Author(s):  
Yury Kostyukevich ◽  
Alexandra Kitova ◽  
Alexander Zherebker ◽  
Shah Rukh ◽  
Eugene Nikolaev
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Molecular Level ◽  
High Resolution Mass Spectrometry ◽  
Historical Records ◽  
Internal Organs ◽  
Funeral Rites ◽  
Wood Pyrolysis ◽  
Orbitrap Mass Spectrometry ◽  
Resolution Mass

Investigation of the archeological material at the molecular level can reveal the composition of ancient paint, balsamic material, reveal cooking recipes, etc. High-resolution mass spectrometry is a powerful technique with underestimated potential for archeology. Here, we present the investigation of the 3000-year-old archeological remains, identified as parts of internal organs of an Egyptian mummy, using high-resolution Orbitrap mass spectrometry. We observed a diverse number of oxidized classes of compounds: O, O2, O3, O4, O5, N, NO, NO2, NO3, NO4, NO5, N2O, N2O2. Such chemical composition is unusual and we never observed it in our previous studies of petroleum, humic substances, products of wood pyrolysis or other natural complex mixtures. It is possible that such compounds are formed via biodegradation of lipids and other organic material used for funeral rites. We did not observe evidence of the presence of mineral bitumen, although there are many historical records of the use of mineral bitumen for mummification.

scholarly journals Liquid chromatography – high resolution mass spectrometry screening of Astragalus hamosus and Astragalus corniculatus

Pharmacia ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 135-139
Author(s):  
Aleksandar Shkondrov ◽  
Ilina Krasteva
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Ionisation Mass Spectrometry ◽  
First Time ◽  
Ionisation Mass ◽  
Resolution Mass

Astragalus hamosus and Astragalus corniculatus were examined for the presence of flavoalkaloids, acylated and highly glycosylated flavonoids. Non-purified extracts of the overground parts of the species were subjected to ultra-high performance liquid chromatography – high resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) analysis and the results were compared to authentic reference substances. A flavoalkaloid of kaempferol was newly identified in an extract of A. hamosus. In addition, three compounds – quercetin and kaempferol flavonoids, acylated with hydroxymethylglutaric acid and alcesefoliside, were found in extracts of A. hamosus and A. corniculatus for the first time.

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