scholarly journals Dry-Cured Ham-Derived Peptide (Asp–Leu–Glu–Glu) Exerts Cytoprotective Capacity in Human Intestinal Epithelial Caco-2 Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1354
Author(s):  
Lujuan Xing ◽  
Lijuan Fu ◽  
Yuejing Hao ◽  
Wangang Zhang
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Activities ◽  
Antioxidant Enzyme Activities ◽  
Intestinal Epithelial ◽  
Protection Effect ◽  
Dry Cured Ham ◽  
Cellular Reactive Oxygen Species

Dry-cured hams are well-known and highly appreciated products in the Mediterranean and China. The long-term fermentation endows dry-cured hams with a unique flavor and quality. Our previous study has identified Asp–Leu–Glu–Glu (DLEE) from dry-cured Xuanwei ham with remarkable antioxidant capacity. In the current study, the Caco-2 cells were cultured in vitro and treated with different doses of DLEE. The cellular reactive oxygen species (ROS) level and antioxidant enzyme activities were then determined to investigate the intracellular protection effect of DLEE. According to the results, the cellular ROS level was reduced, whereas the antioxidant enzyme activities of glutathione reductase, catalase, and glutathione peroxidase were improved following DLEE treatment. The DLEE treatment also increased the Nrf2 expression, along with downregulating the Keap1 expression. Thus, the dry-cured ham-derived peptide DLEE exhibited excellent bioactive capacity by reducing the ROS level and regulating the antioxidant enzyme activities. In addition, Nrf2/Keap1 was shown to be the main signaling pathway underlying DLEE-induced antioxidant activities in Caco-2 cells.

scholarly journals In Vitro Effects of Brassinosteroids on the Growth and Antioxidant Enzyme Activities in Groundnut

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aman Verma ◽  
C. P. Malik ◽  
V. K. Gupta
Keyword(s):  
Antioxidant Enzyme ◽  
Chlorophyll Content ◽  
Enzyme Activities ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Hill Reaction ◽  
Antioxidant Enzyme Activities ◽  
Mda Content ◽  
In Vitro Effects

This paper deals with the in vitro effects of brassinosteroids (BRs) on growth in the form of multiple shoots, chlorophyll content, Hill reaction activity (HRA), activities of catalase (CAT), peroxidase (POX), polyphenol oxidase (PPX), and ascorbate peroxidase (APX) in Arachis hypogaea L. genotypes (M-13 and PBS24030). In vitro impact of BR on shoot multiplication potential was found to be the best at 1 mL L−1 with BA (3 mg L−1) in both the cultivars. Flowering was observed in PBS24030 on the medium containing 2.0 mL L−1 BR with 3 mg L−1 BA. Rhizogenesis was noticed in the presence of BR alone. Total chlorophyll content and HRA were highest at 2.0 mL L−1 with BA in M-13 and 1.0 mL L−1 with BA in PBS24030. Antioxidant enzyme activities were increased in the presence of BR whether alone or in combination with BA in both the cultivars. However, progressive decline was observed in case of MDA content. The results obtained in the study clearly indicated not only the in vitro establishment of groundnut cultivars in the presence of BR alone and in combination with BA but also its effect on various growth promotory physiological parameters.

The effect of a long term (6 months) treatment with (−)deprenyl on antioxidant enzyme activities in selective brain regions in old female fischer 344 rats

1994 ◽  
Vol 47 (8) ◽  
pp. 1333-1338 ◽  
Author(s):  
Maria Cristina Carrillo ◽  
Kenichi Kitani ◽  
Setsuko Kanai ◽  
Yuko Sato ◽  
Kyoko Miyasaka ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Brain Regions ◽  
Antioxidant Enzyme Activities ◽  
Fischer 344 Rats ◽  
Fischer 344

In vitro Antiglycation, Antioxidant Properties of Coleus forskohlii ''Balady'' Leaves and Stem and their Antioxidant Enzyme Activities

2017 ◽  
Vol 19 (3) ◽  
pp. 1-11 ◽  
Author(s):  
Yaaser Almulaiky ◽  
Abudukadeer Kuerban ◽  
Faisal Aqlan ◽  
Saeed Alzahrani ◽  
Mohammed Baeshen ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Properties ◽  
Antioxidant Enzyme Activities ◽  
Coleus Forskohlii

scholarly journals Exploring Antiurolithic Effects of Gokshuradi Polyherbal Ayurvedic Formulation in Ethylene-Glycol-Induced Urolithic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Amol L. Shirfule ◽  
Venkatesh Racharla ◽  
S. S. Y. H. Qadri ◽  
Arjun L. Khandare
Keyword(s):  
Ethylene Glycol ◽  
Calcium Oxalate ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Biochemical Parameters ◽  
Underlying Mechanism ◽  
Antioxidant Enzyme Activities ◽  
Calcium Oxalate Urolithiasis

Gokshuradi Yog (GY)is a polyherbal ayurvedic formulation used traditionally for several decades in India for the treatment of urolithiasis. The aim of the present study was to determine the underlying mechanism ofGYaction in the management of calcium oxalate urolithiasis. The effect ofGokshuradi polyherbal aqueous extracts(GPAEs) was studied on various biochemical parameters involved in calcium oxalate formation by employingin vitroandin vivomethods.GPAEexhibited significant antioxidant activity against 1, 1-diphenyl-2-picrylhydrazyl free radical and inhibited lipid peroxidation in thein vitroexperiments. The rat model of urolithiasis induced by 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) in water caused polyuria, weight loss, impairment of renal function, and oxidative stress and decreased antioxidant enzyme activities in untreated control groups. However,GPAE-(25, 50, and 100 mg/kg) treated groups caused diuresis accompanied by a saluretic effect and revealed significant increase in antioxidant enzyme activities along with decreased oxalate synthesizing biochemical parameters at higher doses. This study revealed the antiurolithic effect ofGPAEmediated possibly through inhibiting biochemical parameters involved in calcium oxalate formation, along with its diuretic and antioxidant effects, hence supporting its use in the treatment of calcium oxalate urolithiasis.

scholarly journals Physiological and biochemical changes in waterlog tolerant sesame genotypes

2017 ◽  
Vol 14 (2) ◽  
pp. 31-45 ◽  
Author(s):  
RR Saha ◽  
F Ahmed ◽  
N Mokarroma ◽  
MM Rohman ◽  
PC Golder
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Antioxidant Activities ◽  
Recovery Period ◽  
Control Plant ◽  
Vegetative Stage ◽  
Antioxidant Enzyme Activities ◽  
Waterlogging Tolerance ◽  
Mda Content ◽  
Sesame Genotypes

An experiment was conducted under pot culture to investigate physiological responses as well as antioxidative enzymes activities that may lead to select sesame genotype (s) which were more waterlogging tolerant at vegetative stage. Four sesame genotypes viz. BD-6980, BD- 6985, BD-6992 and BD-7012 were grown under waterlogged (at vegetative stage) and control (no waterlogged) conditions. Plant height, root length, root volume, root dry weight and leaf area per plant in all the four sesame genotypes significantly decreased due to waterlogging at vegetative stage in comparison to controlled condition. Higher SPAD value (Soil and Plant Analyzer Development) and specific leaf mass were recorded in waterlogged plant than controlled plant during waterlogging period but reverse was the case during recovery period. All the genotypes showed positive indices of waterlogging tolerance in terms of better performance of root, stem, leaf and petiole. Among the genotypes, BD 6980 showed higher waterlogging tolerance in all the components followed by BD 6985. Malondialdehyde (MDA) content was found higher in waterlogged plant of all the four sesame genotypes in both waterlogging period and recovery period than the controlled plant. Antioxidant enzyme activities like Peroxidase (POD), Catalase (CAT), Ascorbate peroxidase (APX), Glutathione peroxidase (GPX) and Superoxide dismutase(SOD) were inconsistent in the present study but most of the antioxidant enzyme activities showed an increasing trend in waterlogged plant than that of control plant in all the genotypes. Comparatively low amount of MDA content and high antioxidant activities of sesame genotype BD 6980 is considered as highly tolerant to waterlogging and other three genotypes are moderately tolerant under water logging condition.SAARC J. Agri., 14(2): 31-45 (2016)

Sign in / Sign up

Export Citation Format

Share Document