scholarly journals Phytochemical screening and antioxidant activity of Melastoma malabathricum and Chromolaena odorata by DPPH radical scavenging method

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 30-37
Author(s):  
N.H. Ismail ◽  
Amira N.H. ◽  
S.N.H.M. Latip ◽  
W.Z.W.M. Zain ◽  
S.N.A. Aani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Chromolaena Odorata ◽  
Melastoma Malabathricum ◽  
Dpph Radical Scavenging

Melastoma malabathricum and Chromolaena odorata are classified under broad-leaved weeds that are widely spread in the open land area. Melastoma malabathricum is commonly known as ‘‘senduduk’’, and C. odorata is locally known as “Pokok Kapal Terbang”. Both weeds are categorized as potential weeds as they have high nutritive value and are rich in chemical compounds. This study aimed to determine their chemical constituents and possible potential for antioxidant activity as these weeds have been reported to possess antioxidant properties. Screening of the plants was performed using standard methods and revealed the existence of various secondary metabolites such as saponins, terpenoids, phenols, tannins, and flavonoids of both weed extracts. Antioxidant activity was validated by the DPPH radical scavenging assay of M. malabathricum and C. odorata crude ethanol extract. The IC50 values for the percentage radical scavenging effects for the extracts were determined. The IC50 value of M. malabatrichum extract was 81.116 μg/mL, C. odorata was 312.903 μg/mL, Vitamin C was 31.023 μg/mL and BHA was 71.521 μg/mL respectively. The study showed that the antioxidant activity of M. malabatrichum was more potent and better than C. odorata.

scholarly journals Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

2020 ◽  
Vol 8 (12) ◽  
pp. 2749-2755
Author(s):  
Mohammad Afzal Hossain ◽  
Nadia Khan Disha ◽  
Jahid Hasan Shourove ◽  
Pappu Dey
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Tannin Content ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

Synthesis, Photophysical, and Antioxidant Properties of Rhodamine B Decorated Novel Dendrimers

2018 ◽  
Vol 71 (6) ◽  
pp. 399 ◽  
Author(s):  
Jothinathan Sathiya Savithri ◽  
Perumal Rajakumar
Keyword(s):  
Antioxidant Activity ◽  
Fluorescence Intensity ◽  
Rhodamine B ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Surface Groups ◽  
Dpph Radical ◽  
Assay Methods ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Novel triazole bridged dendrimers with rhodamine B derivative as surface groups have been achieved using click chemistry by both divergent and convergent approaches. Rhodamine B decorated dendrimers 1, 2, and 3 were synthesised up to the second generation with spirolactam grafted at the terminal. The UV and fluorescence intensity increases with the increase in the dendritic generation. The synthesised rhodamine B decorated dendrimers show significant antioxidant behaviour compared with the standards butylated hydroxy toluene (BHT) and gallic acid when tested by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay and hydroxyl radical scavenging assay methods, respectively. Rhodamine B decorated higher generation dendrimers exhibit better antioxidant activity than the lower generation dendrimers due to the presence of a greater number of triazole branching units and rhodamine B derivative surface units.

scholarly journals Chemical Investigation of SomeCapparisSpecies Growing in Egypt and their Antioxidant Activity

2007 ◽  
Vol 4 (s1) ◽  
pp. 25-28 ◽  
Author(s):  
Ahmed R. Hamed ◽  
Khaled A. Abdel-Shafeek ◽  
Nahla S. Abdel-Azim ◽  
Shams I. Ismail ◽  
Faiza M. Hammouda
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Evaluation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Chemical Investigation ◽  
Dpph Radical ◽  
Densitometric Analysis ◽  
Butanol Fraction ◽  
First Time ◽  
Dpph Radical Scavenging

Capparis cartilagineaandC. desertigrowing in Egypt were investigated for their glucosiolates and rutin content. FromCapparis cartilagineafour isothiocynates were isolated and identified using GC and EI/MS techniques. These compounds were butyl isothiocyanate (1), 6-methylsulphonylhexyl isothiocyanate (2), 7-methylsulphonylheptyl isothiocyanate (3) and 5-benzylsulphonyl-4-pentenyl isothiocyanate (4). In addition to compounds (1) and (2), two other compounds were isolated and identified fromCapparis deserti. These compounds are 3-methylthiopropyl isothiocyanate (5) and [11-(2-butenylthio)6-undecenyl isothiocyanate] (6). Compounds (1), (2), (5) and (6) are reported in this study for the first time fromCapparis deserti. The main flavonoid component in the studied species was isolated and identified as rutin by comparing the data with those reported. Also, quantitative evaluation of rutin in the two species was carried out by TLC-densitometric analysis. The antioxidant activity was done using diphenylpicrylhydrazyl (DPPH) radical scavenging method. The butanol fraction fromC. cartilagineaandC. desertishowed the highest antioxidant properties.

scholarly journals ANTIOXIDATIVE ACTIVITIES AND CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE EXTRACT FROM JASMINUM UNDULATUM KER-GAWL

2011 ◽  
Vol 14 (2) ◽  
pp. 50-57
Author(s):  
Anh Thi Tran ◽  
Binh Thanh Nguyen ◽  
Hoai Thi Cam Ho ◽  
Huong Dang Thien Bui ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidative Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum undulatum Ker Gawl.’s leaves and stems, five fractionss were obtained by partitioning with petroleum ether, chloroform, ethyl acetate and n-butanol solvents. These five fractions were investigated for antioxidative activity using the DPPH radical scavenging and nitric oxide-inhibitory assay. All the fractions showed antioxidative activity except the petroleum ether fraction. Among the fractionss, the ethyl acetate fraction was the most potent fraction in both assays with the SC50 values of 5.30 μg/ml and 80.90 μg/ml, respectively. Further investigation on the eight sub-fractions isolated and extracted from the ethyl acetate fraction showed that one of these sub-fractions, the TE6 sub-fraction, showed the most significant antioxidative activity with the SC50 values of 3.15 μg/ml and 61.83 μg/ml respectively in the DPPH radical scavenging and nitric oxide-inhibitory assay. From the TE4 and TE6 sub-fractions, three compounds were isolated, including p-tyrosol (1), protocatechuic acid (2) and hydroxytyrosol (3). The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, and 2D-NMR.

scholarly journals Antioxidant Activity of Ethanolic Extract from Tandui Leaves (Mangifera rufocostata Kosterm.) by DPPH Radical Scavenging Method

2019 ◽  
Vol 2 (2) ◽  
pp. 114-118
Author(s):  
Revita Saputri ◽  
Tia Mariati Risma Melati ◽  
Fitriyanti Fitriyanti
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Ic50 Value ◽  
Dpph Radical Scavenging

Tandui (Mangifera rufocostata Kosterm.) is a typical plant from South Kalimantan which belongs to the genus of Mangifera. Several species of Mangifera are known to have antioxidant activity. This study aimed to determine the antioxidant activity of ethanol extract of Tandui leaves. Tandui leaves that were obtained from the maceration method used 70% ethanol. Antioxidant activity was conducted quantitative using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The result of the antioxidant activity of Ethanol extract of Tandui leaves quantitatively obtained IC50 value was 60.7042 �g/mL. The ethanol extract of Tandui leaves has strong antioxidant activity

scholarly journals EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

2020 ◽  
pp. 128-131
Author(s):  
RAMBU KONDA A PRAING ◽  
TITIK SUNARNI
Keyword(s):  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Gpx Activity

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model. Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method. Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw. Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark

2018 ◽  
Vol 29 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Oluwafemi Adeleke Ojo ◽  
Adebola Busola Ojo ◽  
Basiru Olaitan Ajiboye ◽  
Babatunji Emmanuel Oyinloye ◽  
Ayodele Jacob Akinyemi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Dependent Manner ◽  
Dpph Radical ◽  
Irvingia Gabonensis ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Phenolic Extracts

AbstractBackground:Irvingia gabonensisstem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis. This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts fromI. gabonensis(Aubry-Lecomte ex O‘Rorke) Baill bark.Methods:Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability. Inhibitory activities on AChE and BChE were evaluated.Results:The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin). The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+to Fe2+) (5.94 mg ascorbic acid equivalent/100 g). Extracts ofI. gabonensisinhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.Conclusions:Hence, possible mechanism through which the stem bark executes their anti-Alzheimer’s disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.

scholarly journals (464) Antioxidant Activity in Lingonberries (Vaccinium vitis-idaea L.)

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090C-1090 ◽  
Author(s):  
Shiow Wang ◽  
Ross Penhallegon
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Soluble Solids ◽  
Oh Radicals ◽  
Dpph Radical ◽  
Phenolic Contents ◽  
Solids Content ◽  
Dpph Radical Scavenging

Lingonberries have been shown to contain high antioxidant activity. Fruit from different cultivars of lingonberry (Vacciniumvitis-idaea L) were evaluated for fruit quality, antioxidant activity, anthocyanin and phenolic contents. The fruit soluble solids content (SSC), titratable acids (TA), antioxidant capacity, anthocyanin and phenolic contents varied with cultivars. The SSC ranged from 12.9% to 16.9%, the TA ranged from 0.31% to 0.41% and the ratios of SSC/TA ranged from 35.37 to 51.21. Lingonberries contain potent free radical scavenging activities for DPPH·, ROO·, ·OH and O .-2 radicals. The oxygen radical absorbance capacity (ORAC) values from acetone extraction of lingonberries ranged from 58.5–223.6 μmol of Trolox equivalents (TE)/g fresh berries. The ED50 values for DPPH-radical scavenging ranged from 5.91–11.77 mg fresh weight. The DPPH-radical scavenging activity correlated with ORAC values with a R2 of 0.8009. ESR spectrum showed that 50 mg·mL-1 of lingonberry extract decreased ·OH radicals by 83% and O .-2 radicals by 99%. Cyanidin 3-galactoside was the most dominant anthocyanin and contributed the most antioxidant activity in lingonberries. The antioxidant properties of lingonberries may play an important role in protecting cells against the oxidative damage caused by free radicals.

scholarly journals Antimicrobial and Antioxidant properties of medicinal mushroom Ganoderma P. Karst

2021 ◽  
Vol 17 (2) ◽  
pp. 106-112
Author(s):  
Anjali Suansia ◽  
Priya John
Keyword(s):  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Xanthomonas Oryzae ◽  
Colletotrichum Falcatum ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Aspergilus Niger

Ganoderma species have been known all over the world as highly medicinal mushrooms. Antimicrobial activity of it is an attractive approach which raises the global interests from the scientific community. In this study, the antimicrobial assay of ethanol and methanol extracts of Ganoderma were prepared by using the dried mycelial powder obtained from five different liquid media; was performed against seven plant pathogenic fungi viz., Alternaria macrospora, Aspergilus niger, A. flavus, Colletotrichum falcatum, Fusarium oxysporum, Pestalotiopsis mangiferae and Penicillium sp. and two plant pathogenic bacteria Xanthomonas oryzae and Ralstonia solanacearum. The extracts of mycelia obtained from Yeast Wine Media exhibited the highest inhibition percentage as compared to rest. At concentration 1000ppm, there was complete inhibition of mycelial growth for Alternaria macrospora, Aspergilus niger and A. flavus while for Fusarium oxysporum and Penicillum sp. complete inhibition was observed at 500ppm but for Colletotrichum falcatum and Pestalotiopsis mangiferae, more than 80% mycelial inhibition at concentration 1000ppm in both ethanol and methanol extracts. In the case of Xanthomonas oryzae and Ralstonia solanacearum, at concentration 1000ppm, methanol extract showed the highest inhibition zone (3.50mm, 3.75mm). Ganoderma exhibited antagonistic effect against plant pathogenic fungi could add to the interest of developing Ganoderma as a successful bioagent in the near future. Antioxidant activities were evaluated by using DPPH radical scavenging assay and Metal chelating activity on ferrous ions. The DPPH radical scavenging effect was detected in methanol extract (Inhibition% = 27.312%) was higher than that of the ethanol extract (Inhibition% = 24.79%) and also Ferrous ion chelating ability of methanol extract (Inhibition% =22.27%) was higher than the ethanol extract (Inhibition% =12.55%). It is clearly indicated that both methanol and ethanol extract of the Ganoderma show antioxidant properties and Ganoderma extracts act as an effective antioxidant agents.

scholarly journals Fatty Acid Analysis, Chemical Constituents, Biological Activity and Pesticide Residues Screening in Jordanian Propolis

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5076
Author(s):  
Rajashri R. Naik ◽  
Ashok K. Shakya ◽  
Ghaleb A. Oriquat ◽  
Shankar Katekhaye ◽  
Anant Paradkar ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Xanthine Oxidase ◽  
Caffeic Acid ◽  
Pesticide Residues ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Propolis is a resinous natural product collected by honeybees (Apis mellifera and others) from tree exudates that has been widely used in folk medicine. The present study was carried out to investigate the fatty acid composition, chemical constituents, antioxidant, and xanthine oxidase (XO) inhibitory activity of Jordanian propolis, collected from Al-Ghour, Jordan. The hexane extract of Jordanian propolis contained different fatty acids, which are reported for the first time by using GC-FID. The HPLC was carried out to identify important chemical constituents such as fatty acids, polyphenols and α-tocopherol. The antioxidant and xanthine oxidase inhibitory activities were also monitored. The major fatty acid identified were palmitic acid (44.6%), oleic acid (18:1∆9cis, 24.6%), arachidic acid (7.4%), stearic acid (5.4%), linoleic acid (18:2∆9–12cis, 3.1%), caprylic acid (2.9%), lignoceric acid (2.6%), cis-11,14-eicosaldienoic acid (20:2∆11–14cis, 2.4%), palmitoleic acid (1.5%), cis-11-eicosenoic acid (1.2%), α–linolenic acid (18:3∆9–12–15cis, 1.1%), cis-13,16-docosadienoic acid (22:2∆13–16cis, 1.0%), along with other fatty acids. The major chemical constituents identified using gradient HPLC-PDA analysis were pinocembrin (2.82%), chrysin (1.83%), luteolin-7-O-glucoside (1.23%), caffeic acid (1.12%), caffeic acid phenethyl ester (CAPE, 0.79%), apigenin (0.54%), galangin (0.46%), and luteolin (0.30%); while the minor constituents were hesperidin, quercetin, rutin, and vanillic acid. The percentage of α-tocopherol was 2.01 µg/g of the lipid fraction of propolis. Antioxidant properties of the extracts were determined via DPPH radical scavenging. The DPPH radical scavenging activities (IC50) of different extracts ranged from 6.13 to 60.5 µg/mL compared to ascorbic acid (1.21 µg/mL). The xanthine oxidase inhibition (IC50) ranged from 75.11 to 250.74 µg/mL compared to allopurinol (0.38 µg/mL). The results indicate that the various flavonoids, phenolic compounds, α-tocopherol, and other constituents which are present in propolis are responsible for the antioxidant and xanthine oxidation inhibition activity. To evaluate the safety studies of propolis, the pesticide residues were also monitored by LC-MS-MS 4500 Q-Trap. Trace amounts of pesticide residue (ng/mL) were detected in the samples, which are far below the permissible limit as per international guidelines.

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