scholarly journals An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 111-116 ◽  
Author(s):  
Tania Santos Giani ◽  
Severo de Paoli ◽  
Giuseppe Antonio Presta ◽  
Adalgisa Ieda Maiworm ◽  
Sebastião David Santos-Filho ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Cellular Membrane ◽  
Blood Constituents ◽  
Technetium 99M ◽  
99Mtc Labeling ◽  
Blood Cell And Plasma

Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated. Buzhong extract at the highest concentrations used altered significantly (p<0.05) the %ATI in blood constituents. Substances present in the Buzhong extract could alter the cellular membrane and/or generation of free radicals that have oxidant properties modifying the labeling of blood constituents with 99mTc.

scholarly journals Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

2007 ◽  
Vol 50 (spe) ◽  
pp. 123-128 ◽  
Author(s):  
Danielle Amorim Terra ◽  
Lúcia de Fátima Amorim ◽  
Maria Tereza Jansen de Almeida Catanho ◽  
Adenilson de Souza da Fonseca ◽  
Sebastião David Santos-Filho ◽  
...  
Keyword(s):  
Blood Cells ◽  
Plasma Proteins ◽  
Trichloroacetic Acid ◽  
Wistar Rats ◽  
Insoluble Fraction ◽  
Artemisia Vulgaris ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M

The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p<0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble fraction). The analysis of the results indicates that the extract could have substances that could interfere on the transport of stannous through the erythrocyte membrane alterating the labeling of blood cells with 99mTc.

scholarly journals Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 175-182 ◽  
Author(s):  
Severo de Paoli ◽  
Tania Santos Giani ◽  
Giuseppe Antonio Presta ◽  
Marcia Oliveira Pereira ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cellular Membrane ◽  
Area Ratio ◽  
Morphometric Parameter ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Clinical Procedures ◽  
Clove Extract ◽  
Blood Fraction

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the morphology of red blood cells by oxidative/chelating actions or interacting with the cellular membrane structure.

Effect of ginkgo biloba on the in vitro labeling of red blood cells and plasma proteins with technetium-99M

2001 ◽  
Vol 44 (S1) ◽  
pp. S639-S641 ◽  
Author(s):  
S. R. F. Moreno ◽  
G. D. Feliciano ◽  
R. S. Freitas ◽  
D. M. Mattos ◽  
M. L. Gomes ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Ginkgo Biloba ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Technetium 99M

scholarly journals Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 153-159 ◽  
Author(s):  
Bernardo Machado Rebello ◽  
Silvana Ramos Farias Moreno ◽  
Camila Godinho Ribeiro ◽  
Rosane de Figueiredo Neves ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Area Ratio ◽  
Passiflora Edulis ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Morphological Evaluation ◽  
Blood Smears ◽  
Ratio Determination

Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and perimeter/area ratio determination. P. flavicarpa extract altered (p<0.05) the fixation of 99mTc on plasma proteins and the perimeter/area ratio of red blood cells. Substances present in P. flavicarpa extract could affect the labeling of blood constituents with 99mTc acting in specific targets as membrane of red blood cells.

scholarly journals Forced Swim Alters the Radiolabeling of Blood Constituents from Wistar Rats

2020 ◽  
Vol 10 (3) ◽  
pp. 1116
Author(s):  
Adenilson S. da Fonseca ◽  
Gabrielle S. Rocha ◽  
Márcia O. Pereira ◽  
André L. B. D. Cardoso ◽  
Eric H. F. F. Frederico ◽  
...  
Keyword(s):  
Blood Cells ◽  
Plasma Proteins ◽  
Wistar Rats ◽  
Acute Stress ◽  
Forced Swimming ◽  
Blood Samples ◽  
Blood Constituents ◽  
Forced Swim ◽  
99Mtc Labeling ◽  
Soluble Fractions

The present study investigated the effects of forced swimming on the technetium-99m (99mTc) labeling of blood constituents (BloCs). Rats (Wistar) were submitted to forced swim. In previous experiments, swimming animals would recover for different periods of time. Animals not submitted to swimming were used as control. Blood samples were obtained and the 99mTc labeling of BloCs was carried out. Blood cells (BCs), plasma (P), insoluble fractions (IF-P and IF-BCs), and soluble fractions (SF-P and SF-BC) were isolated. Radioactivity was determined, and the percentage of 99mTc incorporated (%ATI) was calculated in each fraction. Results showed that forced swimming decreased the percentage of 99mTc incorporated (%ATI) in IF-P (p < 0.05). It is suggested that the 99mTc labeling of BloCs could be used to verify the effects of the stress conditions on BloCs and that the radionuclide fixation on plasma proteins might be altered in rats submitted to acute stress induced by forced swimming, returning to control levels after recovery.

scholarly journals Acetylsalicylic acid and labeling of blood constituents with technetium-99m

2005 ◽  
Vol 48 (spe2) ◽  
pp. 163-168 ◽  
Author(s):  
Adenilson de Souza da Fonseca ◽  
Jacques Natan Grinapel Frydman ◽  
Vanessa Câmara da Rocha ◽  
Mario Bernardo-Filho
Keyword(s):  
Acetylsalicylic Acid ◽  
Whole Blood ◽  
Wistar Rats ◽  
Human Beings ◽  
Blood Constituents ◽  
Technetium 99M ◽  
In Vivo Assays ◽  
99Mtc Labeling

Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99mTc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99mTc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99mTc-labeling process. Saline was used in control groups. Data showed that the fixation of 99mTc to the blood constituents was not significantly (p>0.05) modified in in vitro and in vivo assays with acetylsalicylic acid, at least not when the experiments were carried out with the doses normally used in human beings.

scholarly journals Impact of whole systems traditional Chinese medicine on in-vitro fertilization outcomes

2015 ◽  
Vol 30 (6) ◽  
pp. 602-612 ◽  
Author(s):  
Lee E. Hullender Rubin ◽  
Michael S. Opsahl ◽  
Klaus E. Wiemer ◽  
Scott D. Mist ◽  
Aaron B. Caughey
Keyword(s):  
Chinese Medicine ◽  
In Vitro Fertilization ◽  
Traditional Chinese Medicine ◽  
Vitro Fertilization ◽  
Whole Systems

scholarly journals Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 532
Author(s):  
Hae-Soo Yun ◽  
Sylvatrie-Danne Dinzouna-Boutamba ◽  
Sanghyun Lee ◽  
Zin Moon ◽  
Dongmi Kwak ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Significant Inhibition ◽  
Ic50 Values ◽  
The Republic

In traditional Chinese medicine, Ranunculus japonicus has been used to treat various diseases, including malaria, and the young stem of R. japonicus is consumed as a food in the Republic of Korea. However, experimental evidence of the antimalarial effect of R. japonicus has not been evaluated. Therefore, the antimalarial activity of the extract of the young stem of R. japonicus was evaluated in vitro using both chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains; in vivo activity was evaluated in Plasmodium berghei-infected mice via oral administration followed by a four-day suppressive test focused on biochemical and hematological parameters. Exposure to extracts of R. japonicus resulted in significant inhibition of both chloroquine-sensitive (3D7) and resistant (Dd2) strains of P. falciparum, with IC50 values of 6.29 ± 2.78 and 5.36 ± 4.93 μg/mL, respectively. Administration of R. japonicus also resulted in potent antimalarial activity against P. berghei in infected mice with no associated toxicity; treatment also resulted in improved hepatic, renal, and hematologic parameters. These results demonstrate the antimalarial effects of R. japonicus both in vitro and in vivo with no apparent toxicity.

Phytochemistry and Pharmacology of the Genus Pedicularis Used in Traditional Chinese Medicine

2014 ◽  
Vol 42 (05) ◽  
pp. 1071-1098 ◽  
Author(s):  
Mao-Xing Li ◽  
Xi-Rui He ◽  
Rui Tao ◽  
Xinyuan Cao
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Chemical Constituents ◽  
Iridoid Glycosides ◽  
In Vivo Studies ◽  
Chemical Components ◽  
Wide Range ◽  
Phenylpropanoid Glycosides

In the present review, the literature data on the chemical constituents and biological investigations of the genus Pedicularis are summarized. Some species of Pedicularis have been widely applied in traditional Chinese medicine. A wide range of chemical components including iridoid glycosides, phenylpropanoid glycosides (PhGs), lignans glycosides, flavonoids, alkaloids and other compounds have been isolated and identified from the genus Pedicularis. In vitro and in vivo studies indicated some monomer compounds and extracts from the genus Pedicularis have been found to possess antitumor, hepatoprotective, anti-oxidative, antihaemolysis, antibacterial activity, fatigue relief of skeletal muscle, nootropic effect and other activities.

Wogonin preferentially kills malignant lymphocytes and suppresses T-cell tumor growth by inducing PLCγ1- and Ca2+-dependent apoptosis

Blood ◽  
2008 ◽  
Vol 111 (4) ◽  
pp. 2354-2363 ◽  
Author(s):  
Sven Baumann ◽  
Stefanie C. Fas ◽  
Marco Giaisi ◽  
Wolfgang W. Müller ◽  
Anette Merling ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Therapeutic Potential ◽  
Human T Cell ◽  
Voltage Dependent ◽  
Ca2 Channels ◽  
Malignant T Cells

Herbs have successfully been used in traditional Chinese medicine for centuries. However, their curative mechanisms remain largely unknown. In this study, we show that Wogonin, derived from the traditional Chinese medicine Huang-Qin (Scutellaria baicalensis Georgi), induces apoptosis in malignant T cells in vitro and suppresses growth of human T-cell leukemia xenografts in vivo. Importantly, Wogonin shows almost no toxicity on T lymphocytes from healthy donors. Wogonin induces prolonged activation of PLCγ1 via H2O2 signaling in malignant T cells, which leads to sustained elevation of cytosolic Ca2+ in malignant but not normal T cells. Subsequently, a Ca2+ overload leads to disruption of the mitochondrial membrane. The selective effect of Wogonin is due to its differential regulation of the redox status of malignant versus normal T cells. In addition, we show that the L-type voltage-dependent Ca2+ channels are involved in the intracellular Ca2+ mobilization in T cells. Furthermore, we show that malignant T cells possess elevated amounts of voltage-dependent Ca2+ channels compared with normal T cells, which further enhance the cytotoxicity of Wogonin for malignant T cells. Taken together, our data show a therapeutic potential of Wogonin for the treatment of hematologic malignancies.

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