scholarly journals Effect of the Leavening Agent on the Compositional and Sensorial Characteristics of Bread Fortified with Flaxseed Cake

2020 ◽  
Vol 10 (15) ◽  
pp. 5235 ◽  
Author(s):  
Isabella Taglieri ◽  
Chiara Sanmartin ◽  
Francesca Venturi ◽  
Monica Macaluso ◽  
Angela Zinnai ◽  
...  
Keyword(s):  
Value Added ◽  
Well Being ◽  
Baker’S Yeast ◽  
Dependent Manner ◽  
Baker's Yeast ◽  
Total Phenols ◽  
Sensory Profiles ◽  
Dose Dependent ◽  
Health And Well Being ◽  
By Products

Health and well-being improvement is currently driving innovation in bread, using a wide variety of value-added compounds as extra ingredients, including food industry by-products in a circular economy concept. In this context, this research aimed at evaluating the effect of the fortification of bread with different percentages of flaxseed cake, comparing two leavening agents: sourdough and baker’s yeast. Sensorial, physicochemical, and nutritional properties, including pH, the main fermentative metabolites, fatty acids, total phenols, antioxidant capacity, and volatile organic compounds were determined for fortified bread. The results showed a significant improvement of nutraceutical profile of the bread fortified with flaxseed cake in a dose-dependent manner. Regardless of the leavening agent, the fortification determined a decrease of n-6:n-3 ratio, reaching the recommended value (<3) already at the 7.5% level. Furthermore, under the same fortification level, sourdough breads showed a higher level of total phenols and antiradical activity than baker’s yeast breads. Sensory profiles were instead deeply influenced by both the fortification percentage and the leavening agents. In conclusion, considering both nutritional and sensory results, the best formulation as a function of leavening agent utilized was defined as 5% and 7.5% when sourdough and baker’s yeast were used, respectively.

scholarly journals Autonomously Replicating Linear Plasmids That Facilitate the Analysis of Replication Origin Function inCandida albicans

mSphere ◽  
2019 ◽  
Vol 4 (2) ◽  
Author(s):  
Swati Bijlani ◽  
Mathuravani A. Thevandavakkam ◽  
Hung-Ji Tsai ◽  
Judith Berman
Keyword(s):  
Candida Albicans ◽  
Colony Size ◽  
Transformation Efficiency ◽  
Kluyveromyces Lactis ◽  
Baker’S Yeast ◽  
Dependent Manner ◽  
Baker's Yeast ◽  
Linear Plasmids ◽  
Content Type ◽  
Bona Fide

ABSTRACTThe ability to generate autonomously replicating plasmids has been elusive inCandida albicans, a prevalent human fungal commensal and pathogen. Instead, plasmids generally integrate into the genome. Here, we assessed plasmid and transformant properties, including plasmid geometry, transformant colony size, four selectable markers, and potential origins of replication, for their ability to drive autonomous plasmid maintenance. Importantly, linear plasmids with terminal telomere repeats yielded many more autonomous transformants than circular plasmids with the identical sequences. Furthermore, we could distinguish (by colony size) transient, autonomously replicating, and chromosomally integrated transformants (tiny, medium, and large, respectively).Candida albicansURA3and a heterologous marker,ARG4,yielded many transient transformants indicative of weak origin activity; the replication of the plasmid carrying the heterologousLEU2marker was highly dependent upon the addition of abona fideorigin sequence. Severalbona fidechromosomal origins, with an origin fragment of ∼100 bp as well as a heterologous origin,panARS, fromKluyveromyces lactis, drove autonomous replication, yielding moderate transformation efficiency and plasmid stability. Thus,C. albicansmaintains linear plasmids that yield high transformation efficiency and are maintained autonomously in an origin-dependent manner.IMPORTANCECircular plasmids are important tools for molecular manipulation in model fungi such as baker’s yeast, yet, inCandida albicans, an important yeast pathogen of humans, prior studies were not able to generate circular plasmids that were autonomous (duplicated without inserting themselves into the chromosome). Here, we found that linearizing circular plasmids with sequences from telomeres, the chromosome ends, allows the plasmids to duplicate and segregate inC. albicans. We used this system to identify chromosomal sequences that facilitate the initiation of plasmid replication (origins) and to show that an ∼100-bp fragment of aC. albicansorigin and an origin sequence from a distantly related yeast can both function as origins inC. albicans. Thus, the requirements for plasmid geometry, but not necessarily for origin sequences, differ betweenC. albicansand baker’s yeast.

scholarly journals Survival of Genetically Modified and Self-Cloned Strains of Commercial Baker's Yeast in Simulated Natural Environments: Environmental Risk Assessment

2005 ◽  
Vol 71 (11) ◽  
pp. 7075-7082 ◽  
Author(s):  
Akira Ando ◽  
Chise Suzuki ◽  
Jun Shima
Keyword(s):  
Genetic Engineering ◽  
Freeze Tolerance ◽  
Scientific Data ◽  
Baker’S Yeast ◽  
Natural Environments ◽  
Dependent Manner ◽  
Baker's Yeast ◽  
Viable Cells ◽  
Acid Trehalase ◽  
Pcr Method

ABSTRACT Although genetic engineering techniques for baker's yeast might improve the yeast's fermentation characteristics, the lack of scientific data on the survival of such strains in natural environments as well as the effects on human health prevent their commercial use. Disruption of acid trehalase gene (ATH1) improves freeze tolerance, which is a crucial characteristic in frozen-dough baking. In this study, ATH1 disruptants constructed by genetic modification (GM) and self-cloning (SC) techniques were used as models to study such effects because these strains have higher freeze tolerance and are expected to be used commercially. Behavior of the strains in simulated natural environments, namely, in soil and water, was studied by measuring the change in the number of viable cells and in the concentration of DNA that contains ATH1 loci. Measurements were made using a real-time PCR method during 40 days of cultivation. Results showed that the number of viable cells of GM and SC strains decreased in a time-dependent manner and that the decrease rate was nearly equal to or higher than that for wild-type (WT) yeast. For all three strains (SC, GM, and WT) in the two simulated natural environments (water and soil), the DNA remained longer than did viable cells but the decrease patterns of either the DNA or the viable cells of SC and GM strains had tendencies similar to those of the WT strain. In conclusion, disruption of ATH1 by genetic engineering apparently does not promote the survival of viable cells and DNA in natural environments.

scholarly journals Autonomously replicating linear plasmids facilitate the analysis of replication origin function inCandida albicans

2019 ◽  
Author(s):  
Swati Bijlani ◽  
Mathuravani A. Thevandavakkam ◽  
Hung-Ji Tsai ◽  
Judith Berman
Keyword(s):  
Candida Albicans ◽  
Colony Size ◽  
Transformation Efficiency ◽  
Plasmid Stability ◽  
Kluyveromyces Lactis ◽  
Baker’S Yeast ◽  
Dependent Manner ◽  
Baker's Yeast ◽  
Linear Plasmids ◽  
Bona Fide

AbstractThe ability to generate autonomously replicating plasmids has been elusive inCandida albicans, a prevalent human fungal commensal and pathogen. Instead, plasmids generally integrate into the genome. Here, we assessed plasmid and transformant properties, including plasmid geometry, transformant colony size, four selectable markers, and potential origins of replication for their ability to drive autonomous plasmid maintenance. Importantly, linear plasmids with terminal telomere repeats yielded many more autonomous transformants than circular plasmids with the identical sequences.Furthermore, we could distinguish by colony size, transient, autonomously replicating and chromosomally integrated transformants (tiny, medium and large, respectively).Candida albicans URA3and a heterologous marker,ARG4,yielded many transient transformants indicative of weak origin activity; replication of plasmid carrying heterologousLEU2marker was highly dependent upon the addition of abona fideorigin sequence. Severalbona fidechromosomal origins, with an origin fragment of ~100 bp as well as a heterologous origin,panARS, fromKluyveromyces lactisdrove autonomous replication, yielding moderate transformation efficiency and plasmid stability. Thus,C. albicansmaintains linear plasmids that yield high transformation efficiency and are maintained autonomously in an origin-dependent manner.ImportanceCircular plasmids are important tools for molecular manipulation in model fungi such as baker’s yeast, yet, inCandida albicans, an important yeast pathogen of humans, prior studies were not able to generate circular plasmids that were autonomous (duplicated without inserting themselves into the chromosome). Here, we found that linearizing circular plasmids with sequences from telomeres, the chromosome ends, allows the plasmids to duplicate and segregate inC. albicans.We used this system to identify chromosomal sequences that facilitate the initiation of plasmid replication (origins) and to show that a ~100 bp fragment of aC. albicansorigin, as well as an origin sequence from a distantly related yeast, can both function as origins inC. albicans.Thus, the requirements for plasmid geometry, but not necessarily for origin sequences, differ betweenC. albicansand baker’s yeast.

scholarly journals Impact ofEimeria tenellaCoinfection onCampylobacter jejuniColonization of the Chicken

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Sarah E. Macdonald ◽  
Pauline M. van Diemen ◽  
Henny Martineau ◽  
Mark P. Stevens ◽  
Fiona M. Tomley ◽  
...  
Keyword(s):  
Well Being ◽  
Dependent Manner ◽  
Secondary Effects ◽  
Negative Effects ◽  
Content Type ◽  
Serovar Typhimurium ◽  
The Impact ◽  
Dose Dependent ◽  
Detrimental Impact

ABSTRACTEimeria tenellacan cause the disease coccidiosis in chickens. The direct and often detrimental impact of this parasite on chicken health, welfare, and productivity is well recognized; however, less is known about the secondary effects that infection may have on other gut pathogens.Campylobacter jejuniis the leading cause of human bacterial foodborne disease in many countries and has been demonstrated to exert negative effects on poultry welfare and production in some broiler lines. Previous studies have shown that concurrentEimeriainfection can influence the colonization and replication of bacteria, such asClostridium perfringensandSalmonella entericaserovar Typhimurium. Through a series ofin vivocoinfection experiments, this study evaluated the impact thatE. tenellainfection had onC. jejunicolonization of chickens, including the influence of variations in parasite dose and sampling time after bacterial challenge. Coinfection withE. tenellaresulted in a significant increase inC. jejunicolonization in the cecum in a parasite dose-dependent manner but a significant decrease inC. jejunicolonization in the spleen and liver of chickens. The results were reproducible at 3 and 10 days after bacterial infection. This work highlights thatE. tenellanot only has a direct impact on the health and well-being of chickens but can have secondary effects on important zoonotic pathogens.

scholarly journals Butyrate Supplementation at High Concentrations Alters Enteric Bacterial Communities and Reduces Intestinal Inflammation in Mice Infected with Citrobacter rodentium

mSphere ◽  
2017 ◽  
Vol 2 (4) ◽  
Author(s):  
Janelle A. Jiminez ◽  
Trina C. Uwiera ◽  
D. Wade Abbott ◽  
Richard R. E. Uwiera ◽  
G. Douglas Inglis
Keyword(s):  
Weight Gain ◽  
Tissue Repair ◽  
Well Being ◽  
Enteric Bacteria ◽  
Rectal Administration ◽  
Dependent Manner ◽  
Bacterial Populations ◽  
Content Type ◽  
Butyrate Treatment ◽  
Dose Dependent

ABSTRACT The study findings provide evidence that administration of butyrate in a dose-dependent manner can improve the weight gain of infected mice, enhance clearance of the infection, reduce inflammation through altered cytokine expression, and enhance tissue repair and mucus secretion. Moreover, butyrate treatment also affected the abundance of bacterial populations in both noninflamed and inflamed intestines. Notably, this investigation provides foundational information that can be used to determine the effects of prebiotics and other functional foods on the production of butyrate by enteric bacteria and their impact on intestinal health and host well-being. Butyrate is a short-chain fatty acid by-product of the microbial fermentation of dietary fermentable materials in the large intestine; it is the main energy source for enterocyte regeneration, modulates the enteric microbial community, and contributes to increasing host health via mechanisms that are relatively poorly defined. Limited research has examined the therapeutic potential of butyrate using models of enteric inflammation incited by pathogenic organisms. We used Citrobacter rodentium to incite acute Th1/Th17 inflammation to ascertain the impact of butyrate on the host-microbiota relationship. Rectal administration of 140 mM butyrate to mice increased fecal concentrations of butyrate and increased food consumption and weight gain in mice infected with C. rodentium. Histological scores of colonic inflammation were lower in infected mice administered 140 mM butyrate. Expression of Il10, Tgfβ, and Muc2 was elevated in noninfected mice administered butyrate in comparison to mice not administered butyrate. Infected mice administered butyrate displayed elevated expression of genes necessary for pathogen clearance (i.e., Il17A and Il1β) and of genes involved in epithelial barrier repair and restoration (i.e., Relmβ, Tff3, and Myd88). Butyrate supplemented to inflamed colons increased the abundances of Proteobacteria and Lachnospiraceae and reduced the abundance of Clostridiaceae species. Mice with enteritis that were administered butyrate also exhibited an increased abundance of mucus-associated bacteria. In summary, rectal administration of butyrate increased feed consumption and weight gain, ameliorated C. rodentium-induced cell injury through enhanced expression of immune regulation and tissue repair mechanisms, and increased the abundance of butyrate-producing bacteria in mice with enteritis. IMPORTANCE The study findings provide evidence that administration of butyrate in a dose-dependent manner can increase weight gain in infected mice, enhance clearance of the infection, reduce inflammation through altered cytokine expression, and enhance tissue repair and mucus secretion. Moreover, butyrate treatment also affected the abundance of bacterial populations in both noninflamed and inflamed intestines. Notably, this investigation provides foundational information that can be used to determine the effects of prebiotics and other functional foods on the production of butyrate by enteric bacteria and their impact on intestinal health and host well-being.

scholarly journals Role of Elm1, Tos3, and Sak1 Protein Kinases in the Maltose Metabolism of Baker’s Yeast

2021 ◽  
Vol 12 ◽  
Author(s):  
Xu Yang ◽  
Lu Meng ◽  
Xue Lin ◽  
Huan-Yuan Jiang ◽  
Xiao-Ping Hu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Glucose Repression ◽  
Regulatory System ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Dependent Manner ◽  
Baker's Yeast ◽  
Maltose Metabolism ◽  
New Perspective

Glucose repression is a key regulatory system controlling the metabolism of non-glucose carbon source in yeast. Glucose represses the utilization of maltose, the most abundant fermentable sugar in lean dough and wort, thereby negatively affecting the fermentation efficiency and product quality of pasta products and beer. In this study, the focus was on the role of three kinases, Elm1, Tos3, and Sak1, in the maltose metabolism of baker’s yeast in lean dough. The results suggested that the three kinases played different roles in the regulation of the maltose metabolism of baker’s yeast with differential regulations on MAL genes. Elm1 was necessary for the maltose metabolism of baker’s yeast in maltose and maltose-glucose, and the overexpression of ELM1 could enhance the maltose metabolism and lean dough fermentation ability by upregulating the transcription of MALx1 (x is the locus) in maltose and maltose-glucose and MALx2 in maltose. The native level of TOS3 and SAK1 was essential for yeast cells to adapt glucose repression, but the overexpression of TOS3 and SAK1 alone repressed the expression of MALx1 in maltose-glucose and MALx2 in maltose. Moreover, the three kinases might regulate the maltose metabolism via the Snf1-parallel pathways with a carbon source-dependent manner. These results, for the first time, suggested that Elm1, rather than Tos3 and Sak1, might be the dominant regulator in the maltose metabolism of baker’s yeast. These findings provided knowledge about the glucose repression of maltose and gave a new perspective for breeding industrial yeasts with rapid maltose metabolism.

scholarly journals The Impact of Dietary Curcumin on the Growth Performance, Intestinal Antibacterial Capacity, and Haemato-Biochemical Parameters of Gilthead Seabream (Sparus aurata)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1779
Author(s):  
Ahmed M. Ashry ◽  
Aziza M. Hassan ◽  
Mahmoud M. Habiba ◽  
Ahmed El-Zayat ◽  
Mohamed E. El-Sharnouby ◽  
...  
Keyword(s):  
Growth Performance ◽  
Blood Cells ◽  
Pathogenic Bacteria ◽  
White Blood Cells ◽  
Well Being ◽  
Gilthead Seabream ◽  
Dependent Manner ◽  
Final Body Weight ◽  
The Impact ◽  
Dose Dependent

The need to replace antibiotics in aquafeed is increasing, and alternative safe substances are now encouraged for sustainable aquaculture activity. Curcumin is regarded as a multifunctional feed additive with growth-promoting and immunostimulant potential. Thus, this study evaluated dietary inclusion of curcumin at rates of 0, 1.5, 2, 2.5, and 3% in the diets of Gilthead seabream for 150 days. The results showed an improved final body weight, weight gain, specific growth rate, and feed conversion ratio in fish treated with curcumin, in a dose-dependent manner. The highest growth performance was observed in fish fed a diet supplemented with 3% curcumin. The results also showed lowered activity of pathogenic bacteria (Vibrio spp. and Faecal coliform) in the intestines of Gilthead seabream fed a diet with curcumin inclusion, in a dose-dependent manner. The hematological indices were within the normal range for healthy fish, without meaningful effects except for hematocrit, hemoglobin, red blood cells (RBCs), and white blood cells (WBCs), which were markedly increased by dietary curcumin. Phagocytic activity was obviously enhanced by dietary curcumin, compared with the control. The biochemical blood metabolites related to liver function (alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT)), renal tissue (urea), and total cholesterol were within the normal values, without significant differences. Overall, the inclusion of curcumin at a rate of 2–3% improved the growth performance and well-being of Gilthead seabream.

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