scholarly journals Volatile Organic Compounds of the Glandular Trichomes of Ocimum basilicum and Artifacts during the Distillation of the Leaves

2021 ◽  
Vol 11 (16) ◽  
pp. 7312
Author(s):  
Bruno Tirillini ◽  
Filippo Maggi

Focusing on volatile organic compounds (VOC) of Ocimum basilicum, this study aims to determine the chemical composition of VOC in secretory trichomes and compare it with that of essential oil obtained by hydrodistillation of leaves. The technique of extracting the content of glandular trichomes refers to the microneedle shuttle analysis. Hydrodistillation of fresh leaves was done with a Clevenger distiller (EO). The chemical compositions were determined by GC/FID and GC/MS. The head of the capitate trichomes does not contain volatile compounds. Fifty volatile compounds were detected in the EO, and twenty-four volatile compounds were detected in the VOC; the main components were eugenol (from 15.47% ± 1.05% to 41.89% ± 2.83%) and linalool (from 32.05% ± 2.57% to 28.99% ± 2.32%), respectively. During the distillation of the basil leaves 26 artifacts are formed. The composition of the essential oil of O. basilicum therefore depends not only on the plant but also on the method used to obtain it.

2020 ◽  
Vol 71 (4) ◽  
pp. 136-144
Author(s):  
Irina Ciotlaus ◽  
Maria Pojar-Fenesan ◽  
Ana Balea

The Galium verum herb, (Rubiaceae family) is well represented in the spontaneous Romanian flora. She is one of the most used plants in traditional medicine. Our research aimed to investigate the chemical volatile profile in fresh, dried and essential oil of Gallium verum using SPME and hydrodistillation techniques, followed by GC-MS analysis. Characterization of volatile compounds composition by SPME �GC-MS technique presented din this paper is the first study on fresh and dried of Galium verum plant to our knowledge. Galium verum fresh flower floral bouquet is given by monoterpenes (73,5%), sesquiterpenes (10,16%), esters (10,26%) and others(5.87%). The floral bouquet of Gallium verum dried flower, contains mainly: aldehydes (35.48%) monoterpenes (35.48%), alcohols (11.96%), sesquiterpenes, (3.71%), esters (3.14%) and others (10.11%). Sixty components were identified in the galium essential oil.


2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Sarrou Eirini ◽  
Chatzopoulou Paschalina ◽  
Therios Ioannis ◽  
Dimassi-Theriou Kortessa

Research was carried out in order to evaluate the effect of drought and salinity on Citrus aurantium L. plant physiological characteristics, total phenolic, flavonoid and ascorbic acid contents, and volatile organic compounds. C. aurantium plants were exposed to different levels of drought and salinity for an experimental period of 60 days. Moderate water deficit (MWD) and 100 mM NaCl increased significantly leaf total phenolic, flavonoid and ascorbic acid contents. Both drought and salinity promoted the accumulation of essential oil in leaves, while MWD and 100 mM NaCl resulted in the highest concentrations of essential oil. The main compounds of the essential oil were linalool, linalyl acetate, neryl acetate, geranyl acetate and α-terpineol. MWD and severe water deficit (SWD) reduced the concentration of hydrocarbon monoterpenes and promoted the accumulation of oxygenated compounds, while treatment with 50 and 100 Mm NaCl, promoted the accumulation of hydrocarbon monoterpenes and reduced oxygenated monoterpene concentrations in C. aurantium.


2013 ◽  
Vol 567 ◽  
pp. 9-13
Author(s):  
Y.L. Wang ◽  
Zhi Gang Lu ◽  
P.P. Zhang ◽  
Han Dong Zhou

This study, by means of GC-MS and HPLC, investigated the characteristics of volatile organic compounds (VOCs) released from fiber hot-pressing in a medium density fiberboard (MDF) production line with a production capacity of 80,000 m3/a. The quantity of total volatile organic compounds (TVOC), aldehydes and ketones were calculated. The results showed that the concentration of TVOC reached 1.8940 mg/m3. The main components include terpenes, acrolein and acetone, benzenes series and saturated hydrocarbons. The concentration of the mixture of aldehydes and ketones was 5.1136 mg/m3.


2017 ◽  
Vol 14 (4) ◽  
pp. 1235-1250 ◽  
Author(s):  
Elsa Dilla Dertyasasa ◽  
Woro Anindito Sri Tunjung

ABSTRACT: Previous studies have reported that a number of organic compounds are present in kaffir lime (Citrus hystrix DC.) leaf extracts. Further research is needed to purify these compounds and determine which are biologically active. The objective of this study is to identify the volatile organic compounds of kaffir lime leaf crude extracts and fractions and to study their bioactivity. Fractionation was performed by the double maceration method, using hexane as the second solvent. TLC was performed to analyze the qualitative separation, whereas the individual constituents were detected using GC-MS. Our results showed that chloroform and ethyl acetate crude extracts contained various volatile organic compounds such as fatty acids, fatty alcohols, prenol lipids, sterol lipids, terpenoids and long chain alkanes. Fractionation separated these compounds into non-hexane fractions, which contained less volatile compounds, and hexane fractions. The volatile compounds of non-hexane fractions were identified to be long chain alkanes, meanwhile the hexane fractions contained terpenoids, fatty acids, fatty alcohols, prenol lipids and sterol lipids. Palmitic acid and terpenoids, such as citronellyl propionate, nerolidol, citronella and caryophyllene oxide were found to be the most dominant bioactive compounds in chloroform and ethyl acetate crude extract and their hexane fractions, which were reported to possess cytotoxicity against cancer cells. Meanwhile in non-hexane fractions, long chain alkanes such as triacontane and hentriacontane were found to be the most dominant bioactive compound which also possessed cytotoxic effect. In conclusion, fractionation using the double maceration method yielded different volatile organic compounds composition with different biological activities. The crude extracts and fractions of kaffir lime leaves were potential to be developed as a traditional medicine for cancer treatment.


2021 ◽  
Author(s):  
Chengyi Sun ◽  
Liyun Zhao ◽  
Xue Chen ◽  
Lei Nie ◽  
Aijun Shi ◽  
...  

Abstract China’s current perspective for big cities were filled with great population, great number of restaurants, growing gaseous pollutants and great pollution. Volatile organic compounds (VOCs) were main components of catering industry gaseous pollutants. In this study, we collected and analyzed VOCs from Home cooking (HC), Sichuan and Hunan cuisine (S&H), Shandong cuisine (SD), Anhui cuisine (AH), Beijing cuisine (BJ) and Barbecue (BBQ). The result showed that BBQ gave the highest VOCs concentration (6287.61 µg•m− 3), followed by HC (1806.11 ± 2401.85 µg•m− 3), SD (2238.55 ± 2413.53 µg•m− 3), AH (1745.89 µg•m− 3), S&H (1373.58 ± 1457.45 µg•m− 3) and BJ (288.81 µg•m− 3). The abundance of alkane was higher among BBQ, Anhui Cuisine and HC with the proportion from 33–71%. SD contained higher halohydrocarbons proportion (33%). BJ characterized by high-oxygenated volatile organic compounds proportion (50%). The ozone formation potential (OFP) of BBQ was much greater than other cuisines. The degree of stench pollution from cooking VOCs of HC was 17.51 ± 16.95, followed by S&H (15.77 ± 16.85), SD (15.12 ± 14.17), AH (16.29), BBQ (2.58) and BJ (1.81). Benzene had highest life cancer risk (LCR) in SD (2.11⋅10− 5 ± 3.12⋅10− 5), following HC (4.50⋅10− 6 ± 3.83⋅10− 6) and S&H (4.08⋅10− 6 ± 4.49⋅10− 6). Acrolein had a high hazard index (HI) in HC (789.81 ± 768.77), following AH (728.78), S&H (689.89 ± 776.07), SD (664.29 ± 648.77), BBQ (65.93) and BJ (62.84).


2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Ivan Milovanović ◽  
Aleksandra Mišan ◽  
Jelica Simeunović ◽  
Dajana Kovač ◽  
Dubravka Jambrec ◽  
...  

Microalgal biomass can be used in creating various functional food and feed products, but certain species of microalgae and cyanobacteria are known to produce various compounds causing off-flavour. In this work, we investigated selected cyanobacterial strains ofSpirulina,Anabaena, andNostocgenera originating from Serbia, with the aim of determining the chemical profile of volatile organic compounds produced by these organisms. Additionally, the influence of nitrogen level during growth on the production of volatile compounds was investigated forNostocandAnabaenastrains. In addition, multivariate techniques, namely, principal component analysis (PCA) and hierarchical cluster analysis (HCA), were used for making distinction among different microalgal strains. The results show that the main volatile compounds in these species are medium chain length alkanes, but other odorous compounds such as 2-methylisoborneol (0.51–4.48%), 2-pentylfuran (0.72–8.98%),β-cyclocitral (0.00–1.17%), andβ-ionone (1.15–2.72%) were also detected in the samples. Addition of nitrogen to growth medium was shown to negatively affect the production of 2-methylisoborneol, while geosmin was not detected in any of the analyzed samples, which indicates that the manipulation of growth conditions may be useful in reducing levels of some unwanted odor-causing components.


2015 ◽  
Vol 737 ◽  
pp. 495-498
Author(s):  
Hong Ling Yin ◽  
Hua Wei Yuan ◽  
Zhuang Hu ◽  
Jin Feng Liang ◽  
Shi Ping Li

Volatile organic compounds (VOCs) were analyzed in straw burning period and non-straw burning period in the center of Chengdu city. The results showed that the concentrations of TVOC during straw burning were significantly higher than that in the non-straw burning period in Chengdu. The main components were alkanes, aromatic hydrocarbons and esters in straw burning period while aromatic hydrocarbons, alkanes, esters, aldehydes and ketones as the main contents in the period of non-straw burning. The correlation of TVOC, SO2, NO2, PM10, PM2.5, CO, O3 and the API index was discussed and the NOX-sensitive of photochemical reaction type was determined by the ratio of TVOC / NO2 during straw burning period.


1971 ◽  
Vol 49 (8) ◽  
pp. 1425-1431 ◽  
Author(s):  
Sagar Krupa ◽  
Nils Fries

The mycorrhizal fungus Boletus variegatus Fr. was grown in pure culture and its production of volatile organic compounds studied. Maximal production of volatile organic compounds was associated with actively growing mycelia. The major volatile compounds identified in the culture filtrate were ethanol, isobutanol, isoamyl alcohol, acetoin, and isobutyric acid. Of these, isobutanol and isobutyric acid are known to possess fungistatic activity. Volatile organic compounds were also extracted from the whole root systems of Pinus sylvestris L. (Scots pine) seedlings grown in pure culture with and without the fungal symbiont. Infection of the roots by the fungus resulted in production and (or) accumulation of volatile compounds in concentrations two to eight times greater than that of non-inoculated controls. These compounds were identified by combined gas chromatography and mass spectrometry. They were primarily terpenes and sesquiterpenes. Volatile compounds produced by the mycorrhizal root system of Scots pine collected from a nursery were essentially the same as those obtained from the plants grown in pure culture. Many of these are known to be fungistatic compounds. A hypothesis has been proposed to explain a possible role of the host plant in disease resistance of mycorrhizal root systems to root pathogens and in the development of the symbiotic state.


2009 ◽  
Vol 43 (36) ◽  
pp. 5743-5749 ◽  
Author(s):  
Hua-Hsien Chiu ◽  
Hsiu-Mei Chiang ◽  
Cho-Ching Lo ◽  
Ching-Yen Chen ◽  
Hung-Lung Chiang

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