scholarly journals Flaxseed Lignans and Polyphenols Enhanced Activity in Streptozotocin-Induced Diabetic Rats

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Dan Draganescu ◽  
Calin Andritoiu ◽  
Doina Hritcu ◽  
Gianina Dodi ◽  
Marcel Ionel Popa
Keyword(s):  
Diabetes Management ◽  
Therapeutic Potential ◽  
Plasma Cholesterol ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Flaxseed Lignans ◽  
Food And Water Intake ◽  
Flaxseed Extract ◽  
Positive Results

Flaxseeds play an important role in human health due to their chemical composition and recognized beneficial outcomes. This study investigated the antidiabetic effects of present lignans and polyphenols found in the flaxseed extract on streptozotocin (STZ)-induced diabetic rats. The flaxseed administration produced favorable changes in body weight, food and water intake, and glycosylated hemoglobin and blood glucose quantities in the treated diabetic rats. Additionally, significant positive results were observed in the biochemical parameters, namely reduced plasma cholesterol, LDL cholesterol and triglycerides, plasma creatinine, and urea and uric acid levels, highlighting the seeds’ use in traditional medicine. The results were sustained by histopathological observations that showed better tissue preservation following the flaxseed diet. Overall, the consumption of flaxseeds produced moderate reduction in glucose levels and hyperlipidemia, together with improvement in the impaired organs’ function in diabetic rats. The daily administration of polyphenols and lignans compounds could impact therapeutic potential in diabetes management.

Evaluation of Glucose and Lipid Lowering Activity of Arganimide A in Normal and Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (4) ◽  
pp. 503-510 ◽  
Author(s):  
Mohamed Eddouks ◽  
Farid Khallouki ◽  
Robert W. Owen ◽  
Morad Hebi ◽  
Remy Burcelin
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lowering Activity

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

scholarly journals Efficiency of a formulated condiment (duqqa) in mitigation of diabetes and its complications induced by streptozotocin-nicotinamide in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 218-225
Author(s):  
Rasha S. Mohamed ◽  
Ahmed M. Abdel-Salam
Keyword(s):  
Diabetes Management ◽  
Diabetic Control ◽  
Black Pepper ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Balanced Diet ◽  
Liver And Kidney ◽  
Onset Of Diabetes ◽  
Date Seeds Powder ◽  
Kidney Functions

Introduction: Duqqa is a condiment, consisting of black pepper, cumin, sesame, coriander and high amount of salt. Reducing salt and adding other beneficial items to traditional duqqa can make it suitable dietary supplement for diabetes management. The current study aimed to assess the effect of a modified duqqa on diabetes and its complications in diabetic rats. Methods: The modified duqqa was formulated by mixing grounded fermented wheat, sesame, coriander, cumin, chicory leaves, cinnamon, turmeric and date seeds powder and studied in diabetic rats which were developed by streptozotocin-nicotinamide injection. Thirty-two rats were divided into four groups (n = 8) including non-diabetic, diabetic control and the other two groups fed on balanced diet supplemented with either 10 or 20% of duqqa prior the induction of diabetes (for one week) to the end of the experiment (8 weeks). Results: The dietary supplementation with 10 and 20% of the formulated duqqa prior the induction of diabetes did not delay the onset of diabetes in rats but produced reduction (32.56% and 50.47%, respectively) in the glucose levels of diabetic rats. Also, diabetic rats fed on the formulated duqqa showed insulin concentrations higher than that of diabetic control rats. Feeding diabetic rats on the formulated duqqa reversed the elevation of kidney lipid peroxidation and nitric oxide, limited the disturbance in the lipid profile as well as liver and kidney functions and elevated both serum and femur magnesium concentrations. Conclusion: The results indicated the hypoglycemic effect of the formulated duqqa and its efficiency in delaying diabetes complications.

scholarly journals Antidiabetic and antihyperlipidemic activities of extracts of Barleria cuspidata Heyne ex Nees on streptozotocin induced diabetic rats

2021 ◽  
Vol 12 (1) ◽  
pp. 643-652
Author(s):  
Manohar Reddy ◽  
Raja Sundararajan
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glycosylated Hemoglobin ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Serum Triglycerides ◽  
Methanol Extracts ◽  
Glucose Levels ◽  
Blood Glucose Levels

Traditionally, Barleria cuspidata Heyne ex Nees is utilized for antidiabetic action with the absence of logical investigation. Thus, the current examination was attempted to explore for its antidiabetic and antihyperlipidemic movement in streptozotocin instigated diabetic animal models. Blood glucose levels were estimated in normoglycemic rats at initial, 60th and 120th minutes intervals and in glucose feed hyperglycemic rats at initial, 30, 60, 90 and 120 minutes after a solitary portion of streptozotocin at 55 mg/kg body weight intraperitoneal were made diabetic in albino rats. Blood glucose levels were estimated at week by week spans after everyday administration of chloroform and methanol extracts of Barleria cuspidata at dosages of 250 and 500 mg/kg body weight. Other biochemical boundaries of serum triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, total protein, albumin, globulin, uric acid, creatinine, urea, transaminases, alkaline phosphatase, alanine aminotransaminase, insulin and glycosylated hemoglobin were likewise estimated toward the finish of the investigation. Chloroform and methanol extracts of Barleria cuspidata by an oral organization for 21 days altogether (P<0.001) decreases the elevated blood glucose extents in diabetic rats whereas in normoglycemic rats it doesn't adjust the blood glucose amounts altogether and in glucose feed hyperglycemic rats significantly decreases the raised blood glucose levels. Likewise, the chloroform and methanol extracts of Barleria cuspidata improved other biochemical boundaries related to diabetes. Moreover, the extracts of Barleria cuspidata favourable affect the histopathological changes of pancreas in streptozotocin initiated diabetic rats. Delayed consequences legitimize the traditional utilization of Barleria cuspidata for its anti-diabetic action.

The Treatment of Prednisone in Mild Diabetic Rats: Biochemical Parameters and Cell Response

2020 ◽  
Vol 20 (5) ◽  
pp. 797-805 ◽  
Author(s):  
Mariana P.R. Machado ◽  
Aline Z. Schavinski ◽  
Amanda L. Deluque ◽  
Gustavo T. Volpato ◽  
Kleber E. Campos
Keyword(s):  
Body Weight ◽  
Adult Life ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Insulin Tolerance ◽  
Mild Diabetes ◽  
Food And Water Intake

Background: Limited studies have been carried out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of β-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM+PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and immunoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high-density and very lowdensity lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should be replaced in order to not to aggravate this condition.

scholarly journals Protective Effects of the MushroomLactarius deterrimusExtract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Mirjana Mihailović ◽  
Jelena Arambašić Јovanović ◽  
Aleksandra Uskoković ◽  
Nevena Grdović ◽  
Svetlana Dinić ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pancreatic Islets ◽  
Diabetes Management ◽  
Therapeutic Potential ◽  
Cell Mass ◽  
Diabetic Rats ◽  
Nuclear Antigen ◽  
Protective Effects ◽  
Systemic Oxidative Stress

The aim of this study was to assess thein vivoeffects of the extract of the medicinal mushroom,Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with theL. deterrimusextract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with theL. deterrimusextract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration ofβ-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positiveβ-cells were increased. These results show that the ability of theL. deterrimusextract to alleviate oxidative stress and increaseβ-cell mass represents a therapeutic potential for diabetes management.

scholarly journals In Vivo Assessment of Antioxidants and Antihyperglycemic Effect of Barleria cristata leaves in Streptozotocin- Induced Diabetic Rats

2014 ◽  
Vol 2 (4) ◽  
pp. 437-445 ◽  
Author(s):  
Narmadha Rajasekaran ◽  
Gomathi Duraisamy ◽  
Kalaiselvi Manokaran ◽  
Devaki Kanakasapathi
Keyword(s):  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Leaf Extracts ◽  
Oral Hypoglycaemic Agents ◽  
Standard Drug ◽  
C Peptide ◽  
Glucose Levels ◽  
Glycemic Profile ◽  
Nonenzymatic Antioxidants

Objective: Many new bioactive drugs isolated from plants having hypoglycaemic effects showed anti diabetic activity equal and sometimes even more potent than known oral hypoglycaemic agents. In this present study, designed to evaluate antihyperglycermic and antioxidants effect on ethanolic leaf extracts Barleria cristata (EtBc) in streptozotocin-induced diabetic rats at dose level 400mg/kg body weight for the treatment of 45 days. Method and materials: The experimental rats were randomly divided into five groups as a control, streptozotocin induced with diabetes (45mg/kg bw) without any treatment, treated with standard drug glibenclamide (1.25 mg/kg bw), EtBc (400 mg/kg bw) in diabetic induced rats and treated with EtBc alone without diabetic rats. At the end of 45th day animals were sacrificed, collect the serum, liver, kidney and pancreas for estimate the glucose, insulin, C-peptide, glycosylated hemoglobin, hemoglobin in serum, protein, enzymatic and nonenzymatic antioxidants and lipid peroxidation in tissues. Results: After the administration of EtBc, blood glucose levels were showed significantly reduction (P<0.05) in diabetic rats and it has been observed alternation occured in body and organ weight and it was also normalized the serum level of glycemic profile like insulin, C-peptide, total hemoglobin and glycosylated hemoglobin levels similar to that of control rats. Antioxidants enzymes were return to back their levels as control in different tissues when compared to diabetic rats and also observed no significance difference between control and EtBc alone group rats at the end of 45th day. Therefore it was suggested that Barleria cristata may act by potentiation of pancreatic secretion of insulin or increasing glucose uptake by muscle cells. Conclusion: In this study, suggested the efficacy of Barleria cristata proved the maintenance of glucose homeostasis and may be used as a therapeutic agent in the management of diabetes mellitus.DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11219 Int J Appl Sci Biotechnol, Vol. 2(4): 437-445 

scholarly journals Ginsenoside Rg1 Alleviates Podocyte EMT Passage by Regulating AKT/GSK3 β/β-Catenin Pathway by Restoring Autophagic Activity

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yimin Shi ◽  
Yanbin Gao ◽  
Tao Wang ◽  
Xiaolei Wang ◽  
Jiaxin He ◽  
...  
Keyword(s):  
High Glucose ◽  
Epithelial Mesenchymal Transition ◽  
Therapeutic Potential ◽  
Diabetic Rats ◽  
Ginsenoside Rg1 ◽  
Glomerular Diseases ◽  
Mesenchymal Transition ◽  
Glucose Levels ◽  
Autophagic Activity

Background. Diabetic nephropathy (DN), a complication of diabetes, is the result of high glucose-induced pathological changes in podocytes, such as epithelial-mesenchymal transition (EMT). Autophagy is an important mechanism of podocyte repair. Ginsenoside Rg1, the active ingredient of ginseng extract, has antifibrotic and proautophagic effects. Therefore, we hypothesized that ginsenoside Rg1 can reverse podocyte EMT via autophagy and alleviate DN. Aim. This study aimed to investigate the effect of ginsenoside Rg1 on DN rats and high glucose-induced podocyte EMT by regulating the AKT/GSK3β/β-catenin pathway by restoring autophagy activity. Methods. Diabetic rats induced by STZ injection were treated with 50 mg/kg ginsenoside Rg1 for 8 weeks, and the renal functional, metabolic, and histopathological indices were evaluated. DN was simulated in vitro by exposing podocytes to high glucose levels and treated with ginsenoside Rg1. The expression of EMT and autophagy-related markers was analyzed in vivo and in vitro by immunofluorescence, western blotting, and real-time PCR. Results. Ginsenoside Rg1 significantly alleviated renal fibrosis and podocyte EMT in diabetic rats, and podocytes exposed to high glucose levels, which was abolished by the autophagy inhibitor 3-MA. Furthermore, ginsenoside Rg1 regulated the AKT/GSK3 β/β-catenin pathway by restoring autophagic activity. Conclusion. Ginsenoside Rg1 alleviated podocyte EMT by enhancing AKT/GSK3β/β-catenin pathway-mediated autophagy, indicating its therapeutic potential for DN and other glomerular diseases.

scholarly journals Core structure of flavonoids precursor as an antihyperglycemic and antihyperlipidemic agent: an in vivo study in rats.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Mahmoud Najafian ◽  
Azadeh Ebrahim-Habibi ◽  
Parichehreh Yaghmaei ◽  
Kazem Parivar ◽  
Bagher Larijani
Keyword(s):  
Beneficial Effect ◽  
Therapeutic Potential ◽  
Urine Volume ◽  
Diabetic Rats ◽  
Core Structure ◽  
Alpha Amylase ◽  
Glucose Levels ◽  
Blood Glucose Levels

trans-Chalcone is the core structure of naringenin chalcone, located halfway in the biosynthesis pathway of flavonoids. Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and related disorders. As a mammalian alpha-amylase inhibitor in vitro, the putative beneficial effect of trans-chalcone on diabetes was tested in a streptozotocin-induced rat model of diabetes type 1, and the results analyzed with commonly used statistical methods. Significant reduction of blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. trans-chalcone was observed to have a weight loss-inductive effect, alongside with a reduction in food intake, which is suggestive of a therapeutic potential of this compound in overweight and obese patients.

scholarly journals Multivesicular Liposomes for Glucose-Responsive Insulin Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 21
Author(s):  
Guangqu Liu ◽  
Suping He ◽  
Yu Ding ◽  
Cai Chen ◽  
Qingchun Cai ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Diabetes Management ◽  
Double Emulsion ◽  
Insulin Delivery ◽  
Diabetic Rats ◽  
Alizarin Red ◽  
Glucose Levels ◽  
Chemically Induced

An intelligent insulin delivery system is highly desirable for diabetes management. Herein, we developed a novel glucose-responsive multivesicular liposome (MVL) for self-regulated insulin delivery using the double emulsion method. Glucose-responsive MVLs could effectively regulate insulin release in response to fluctuating glucose concentrations in vitro. Notably, in situ released glucose oxidase catalyzed glucose enrichment on the MVL surface, based on the combination of (3-fluoro-4-((octyloxy)carbonyl)phenyl)boronic acid and glucose. The outer MVL membrane was destroyed when triggered by the local acidic and H2O2-enriched microenvironment induced by glucose oxidase catalysis in situ, followed by the further release of entrapped insulin. Moreover, the Alizarin red probe and molecular docking were used to clarify the glucose-responsive mechanism of MVLs. Utilizing chemically induced type 1 diabetic rats, we demonstrated that the glucose-responsive MVLs could effectively regulate blood glucose levels within a normal range. Our findings suggest that glucose-responsive MVLs with good biocompatibility may have promising applications in diabetes treatment.

scholarly journals Antidiabetic Effect and Mode of Action of Cytopiloyne

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Cicero Lee-Tian Chang ◽  
Hsien-Yueh Liu ◽  
Tien-Fen Kuo ◽  
Yi-Jou Hsu ◽  
Ming-Yi Shen ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Blood Glucose ◽  
Mechanism Of Action ◽  
Mode Of Action ◽  
Therapeutic Potential ◽  
Glycosylated Hemoglobin ◽  
Postprandial Blood Glucose ◽  
Blood Insulin ◽  
Glucose Levels ◽  
Long Term Treatment

Cytopiloyne was identified as a novel polyacetylenic compound. However, its antidiabetic properties are poorly understood. The aim of the present study was to investigate the anti-diabetic effect and mode of action of cytopiloyne on type 2 diabetes (T2D). We first evaluated the therapeutic effect of cytopiloyne on T2D in db/db mice. We found that one dose of cytopiloyne reduced postprandial glucose levels while increasing blood insulin levels. Accordingly, long-term treatment with cytopiloyne reduced postprandial blood glucose levels, increased blood insulin, improved glucose tolerance, suppressed the level of glycosylated hemoglobinA1c(HbA1c), and protected pancreatic islets in db/db mice. Next, we studied the anti-diabetic mechanism of action of cytopiloyne. We showed that cytopiloyne failed to decrease blood glucose in streptozocin- (STZ-)treated mice whoseβcells were already destroyed. Additionally, cytopiloyne dose dependently increased insulin secretion and expression inβcells. The increase of insulin secretion/expression of cytopiloyne was regulated by protein kinase Cα(PKCα) and its activators, calcium, and diacylglycerol (DAG). Overall, our data suggest that cytopiloyne treats T2D via regulation of insulin production involving the calcium/DAG/PKCαcascade inβcells. These data thus identify the molecular mechanism of action of cytopiloyne and prove its therapeutic potential in T2D.

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