scholarly journals Efficiency of a formulated condiment (duqqa) in mitigation of diabetes and its complications induced by streptozotocin-nicotinamide in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 218-225
Author(s):  
Rasha S. Mohamed ◽  
Ahmed M. Abdel-Salam
Keyword(s):  
Diabetes Management ◽  
Diabetic Control ◽  
Black Pepper ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Balanced Diet ◽  
Liver And Kidney ◽  
Onset Of Diabetes ◽  
Date Seeds Powder ◽  
Kidney Functions

Introduction: Duqqa is a condiment, consisting of black pepper, cumin, sesame, coriander and high amount of salt. Reducing salt and adding other beneficial items to traditional duqqa can make it suitable dietary supplement for diabetes management. The current study aimed to assess the effect of a modified duqqa on diabetes and its complications in diabetic rats. Methods: The modified duqqa was formulated by mixing grounded fermented wheat, sesame, coriander, cumin, chicory leaves, cinnamon, turmeric and date seeds powder and studied in diabetic rats which were developed by streptozotocin-nicotinamide injection. Thirty-two rats were divided into four groups (n = 8) including non-diabetic, diabetic control and the other two groups fed on balanced diet supplemented with either 10 or 20% of duqqa prior the induction of diabetes (for one week) to the end of the experiment (8 weeks). Results: The dietary supplementation with 10 and 20% of the formulated duqqa prior the induction of diabetes did not delay the onset of diabetes in rats but produced reduction (32.56% and 50.47%, respectively) in the glucose levels of diabetic rats. Also, diabetic rats fed on the formulated duqqa showed insulin concentrations higher than that of diabetic control rats. Feeding diabetic rats on the formulated duqqa reversed the elevation of kidney lipid peroxidation and nitric oxide, limited the disturbance in the lipid profile as well as liver and kidney functions and elevated both serum and femur magnesium concentrations. Conclusion: The results indicated the hypoglycemic effect of the formulated duqqa and its efficiency in delaying diabetes complications.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

Effect of Garlic Oil on the Levels of Various Enzymes in the Serum and Tissue of Streptozotocin Diabetic Rats

2001 ◽  
Vol 21 (1) ◽  
pp. 19-24 ◽  
Author(s):  
O. C. Ohaeri
Keyword(s):  
Serum Amylase ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Red Cell ◽  
Alkaline Phosphatases ◽  
Garlic Oil ◽  
Acid And Alkaline Phosphatase ◽  
Liver And Kidney ◽  
Streptozotocin Diabetic Rats ◽  
Aspartate Transferase

Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values of tissue amylase and total protein were also assessed from the pancreas, liver, and kidney. Treatment of diabetic rats with garlic oil significantly decreased the red cell phosphatase (p<0.01), serum acid and alkaline phosphatase (p<0.001) when compared to diabetic control rats. Serum alanine and asparate transferases were significantly (p<0.001) decreased as well as serum amylase (p<0.002) in garlic oil treated diabetic rats as compared with diabetic control rats. When treated with garlic oil, however, diabetic and normal rats showed significant increase (p<0.05) in the amylase levels of the pancrease, liver, and kidney.

scholarly journals Anti-diabetic effects of aqueous extract of Dendropanax morbifera Lev. leaves in streptozotocin-induced diabetic Sprague-Dawley rats

2021 ◽  
Vol 61 (4) ◽  
pp. e38
Author(s):  
Min-Jae Kim ◽  
Ye-Jin Kang ◽  
Dong-Eon Lee ◽  
Suk Kim ◽  
Se-Hun Lim ◽  
...  
Keyword(s):  
Diabetic Control ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
Glucose Levels ◽  
Blood Biochemical ◽  
Blood Glucose Levels ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Dendropanax Morbifera ◽  
Treatment Of Diabetes

This study examined the anti-diabetic effects of aqueous extracts of Dendropanax morbifera leaves (DMWEs) in streptozotocin-induced diabetic Sprague-Dawley (SD) rats. Thirty male SD rats (body weight [BW], 250.4 ± 19.7 g) were divided into the following six groups: normal control rats (NC), diabetic control rats (DC), diabetic rats treated with metformin HCl 100 mg/kg BW (DT), diabetic rats treated with DMWEs 50 mg/kg BW (DM-50), diabetic rats treated with DMWEs 100 mg/kg BW (DM-100), and diabetic rats treated with DMWEs 200 mg/kg BW (DM-200). From two weeks of administration of DMWEs, the BW of all groups treated with DMWEs increased significantly compared to DC (p < 0.05). At four weeks after treatment, the blood glucose levels in DT, DM-100, and DM-200 decreased below 200 mg/dL, while the glycated hemoglobin concentrations in all groups administered DMWEs were similar to those of NC and DT. Regarding the blood biochemical parameters, the levels of aspartate transaminase, alanine transaminase, blood urea nitrogen, and creatinine in DM-100 and DM-200 were similar to those in NC and DT. Overall, these results highlight the effectiveness of DM-100 in the treatment of diabetes.

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECTS OF THE ETHANOL EXTRACT OF THE LEAVES AND STEM OF CISSUS GRACILLIS (GULL ET PERR) (VITACEAE)

2021 ◽  
pp. 54-56
Author(s):  
OLUSAYO A SHORINWA ◽  
GORDON EI EMENU
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Diabetic Control Group ◽  
Alloxan Monohydrate

Objectives: This study investigated the antidiabetic and antihyperlipidemic potential of the ethanol extract of the leaves and stem of Cissus gracillis on alloxan monohydrate-induced diabetic albino rats. Methods: Preliminary phytochemical screening and acute toxicity were carried out. Animals were assigned into seven groups of five rats each. Groups A and B were administered 10 mg/kg each of glibenclamide and atorvastatin respectively, C, D, and E were given 125, 250 and 500 mg/kg of ethanol extract of C. gracillis, respectively, daily for 21 days through oral gavage, group F was diabetic but untreated (diabetic control group), while group G was non-diabetic and untreated which served as the control group. Results: Phytochemical screening revealed the presence of steroids/triterpenoids and carbohydrates. LD50 was above 5000 mg/kg. The extract at 500 mg/kg showed a statistically significant (p<0.05) decrease in blood glucose level when compared with the glibenclamide group on day 21. However, gradual non- significant reduction in blood glucose levels were observed in the extract treated groups on the 7th, 14th, and 21st days of treatment. The administration of ethanol extract of C. gracillis to alloxan-induced diabetic rats produced a decrease in total cholesterol, triglycerides, and low-density lipoproteins comparable to glibenclamide and atorvastatin. Conclusion: The ethanol extract of the leaves and stem of C. gracillis possess a mildly significant antidiabetic and antihyperlipidemic activity.

scholarly journals Antidiabetic activity of curry leaves Murraya koenigii on glucose level, kidney and islets of langerhans in streptozotocin induced diabetes in rats

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Imad M. Al-Ani ◽  
Rahajoe I. Santosa ◽  
Muhammad H. Yankuzo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Murraya Koenigii ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Curry Leaves ◽  
Aqueous Leaf Extract

Introduction: This study examined the antihyperglycemic effect of curry leaves, Murraya koenigii “MK” aqueous extract, and to examine its possible protective effects on the Islets of Langerhans and kidneys in streptozotocin (STZ) induced diabetic rats.  Methods: Thirty healthy adult male Sprague Dawley rats were randomized into five groups (n=6); normal control, normal treated with “MK” control, diabetic control (non-treated with “MK”), diabetic treated with 200mg/kg MK aqueous leaf extract and diabetic treated with 400mg/kg MK aqueous leaf extract. Blood glucose levels and body weight were monitored. The animals were sacrificed on the 30th day; the kidney and pancreatic tissues were processed for histological studies. Results: The diabetic control group significantly (p<0.001) showed considerable loss of body weight and increase in blood glucose levels and degeneration of the glomeruli and renal convoluted tubules and atrophied islets with disintegration of β-cells. Treatment of diabetic rats with aqueous extract showed significant (p<0.001) improvement in blood glucose levels and body weight gain.  The MK extract also caused an improvement in tissue injury induced by STZ injection in the kidney and endocrine pancreas.  Conclusions: These findings highlighted the beneficial effects of MK aqueous extract against cellular oxidative damage in STZ-induced diabetic rats.

scholarly journals ANTIDIABETIC EFFECT OF MIKANIA SCANDENS ON ALLOXAN-INDUCED RAT MODEL

2018 ◽  
Vol 11 (3) ◽  
pp. 109
Author(s):  
Rimashree Baishya ◽  
Anjan Adhikari ◽  
Sharmistha Biswas ◽  
Soma Banerjee
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Standard Drug ◽  
Glucose Levels ◽  
Male Wistar Rats ◽  
Histopathological Studies ◽  
Alloxan Monohydrate

 Objective: The objective was to study the evaluation of antidiabetic and antioxidant activity of different doses ethanolic extract of Mikania scandens leaves.Methods: The rats were divided into five groups of six animals each (non-diabetic control, diabetic control, standard drug-treated, and plant treated groups (250 and 500 mg/kg body weight)). Diabetes was induced in the healthy male Wistar rats (150–200 g body weight, 4–6 weeks old) by the administration of alloxan monohydrate (150 mg/kg, i.p.). On the 0th, 3th, 7th, 11th, and 15th days, the blood samples were analyzed for blood glucose. The antioxidant and antidiabetic parameters were evaluated by standard protocol. The liver tissue was used for histopathological assessment of liver damage.Results: The ethanolic extract of leaves of M. scandens showed a significant reduction (p<0.001) in the blood glucose levels and the antioxidant levels, with 500 mg/kg of body weight in the alloxan-induced diabetic rats as compared to the controls. Histopathological studies provided evidence that the leaf extract possesses antidiabetic activity.Conclusions: The ethanolic extract of leaves of M. scandens showed potent antidiabetic and antioxidant abilities against alloxan-induced rats, which indicate the presence of hypoglycemic constituents in the plant.

Dioscorea dumetorum-Fed Rats Exhibited Decreased Body Weight, Blood Glucose, and Insulin in STZ-induced Diabetes

2014 ◽  
Vol 4 (2) ◽  
pp. 87 ◽  
Author(s):  
Henrietta Aritetsoma Ogbunugafor ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Chinwe Nonyelum Ezekwesili ◽  
Chike Samuel Okafor ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Feed Intake ◽  
Weight Reduction ◽  
Blood Glucose Concentration ◽  
Insulin Level ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Preventive measures that could slow down the rising incidences of diabetes mellitus are essential. The use of neglected local foods, which have effects on this chronic disease beyond basic nutrition as dietary controls, is desirable.Objective: The effect of Dioscorea dumetorum (Kunth) Pax (Dioscoreaceae) feed on satiety, weight, blood glucose, and insulin levels were investigated in streptozotocin-induced diabetic rats.Methods: Twenty adult male rats in four groups of five were used for the experiment. Three groups – D. dumetorum, glibenclamide, and standard pellet-fed rats were induced with diabetes by i.p. administration of 50mg kg-1 streptozotocin, while the fouth group (?) served as a non-diabetic control. D. dumetorum was fed at 15g daily for ten days before induction, and after induction, feeding continued. Glibenclamide was orally administered 5mg kg-1 daily. Both the untreated and non-diabetic rats were kept on standard rat pellets. Feed intake, weight, and blood glucose concentration were monitored daily, while insulin level was measured on day two and day six after inductions. Results: Average feed intake for non-diabetic rats was 15g for D. dumetorum per day, which dropped to 10.3g after induction of diabetes. Weight of normal non-diabetic rats consistently increased (142.61 ± 4.37g – 169.43 ± 8.61g) for the duration (17 days) of the experiment. The D. dumetorum-fed rats showed weight reduction of 5.4%, glibenclamide 4.0%, and untreated diabetic 6.15%. Non-diabetic rats blood glucose levels ranged between 70 to 100mg dL-1. Streptozotocin (STZ) (i.p.) administration increased blood glucose levels from 370% to 626% in the rats. D. dumetorum-fed rats showed reduced (p<0.05) blood glucose levels of 22.6%. Glibenclamide had 5.5% reduction (p<0.05). Insulin was absent in D. dumetorum-fed rats, whereas 0.95ng ml-1 of insulin was detected in glibenclamide-administered rats. These quantities were lower (p<0.001) than 1.40ng ml-1 in the non-diabetic rats. Conclusion: This study revealed that D. dumetorum tuber caused decreased hunger, weight reduction, and displayed hypoglycemic property in diabetic rats, even after heat treatment. Its probable mechanism of anti-hyperglycemic activity might not be through increased insulin secretion.Key words: D. dumetorum, streptozotocin-induced diabetes, weight, blood glucose, insulin.

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