scholarly journals Comparison of Culturing and Metabarcoding Methods to Describe the Fungal Endophytic Assemblage of Brachypodium rupestre Growing in a Range of Anthropized Disturbance Regimes

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1246
Author(s):  
María Durán ◽  
Leticia San Emeterio ◽  
Rosa Maria Canals
Keyword(s):  
Fungal Diversity ◽  
Fungal Endophytes ◽  
Complete Characterization ◽  
Large Area ◽  
Tall Grass ◽  
Disturbance Regimes ◽  
Natural Grasslands ◽  
Low Diversity ◽  
High Diversity

Fungal endophytes develop inside plants without visible external signs, and they may confer adaptive advantages to their hosts. Culturing methods have been traditionally used to recognize the fungal endophytic assemblage, but novel metabarcoding techniques are being increasingly applied. This study aims to characterize the fungal endophytic assemblage in shoots, rhizomes and roots of the tall grass Brachypodium rupestre growing in a large area of natural grasslands with a continuum of anthropized disturbance regimes. Seven out of 88 taxa identified via metabarcoding accounted for 81.2% of the reads (Helotiaceae, Lachnum sp. A, Albotricha sp. A, Helotiales A, Agaricales A, Mycena sp. and Mollisiaceae C), revealing a small group of abundant endophytes and a large group of rare species. Although both methods detected the same trends in richness and fungal diversity among the tissues (root > rhizome > shoot) and grasslands (low-diversity > high-diversity grasslands), the metabarcoding tool identified 5.8 times more taxa than the traditional culturing method (15 taxa) but, surprisingly, failed to sequence the most isolated endophyte on plates, Omnidemptus graminis. Since both methods are still subject to important constraints, both are required to obtain a complete characterization of the fungal endophytic assemblage of the plant species.

Experimental Characterization of Mechanical Behavior of Cord-Rubber Composites

1982 ◽  
Vol 10 (1) ◽  
pp. 37-54 ◽  
Author(s):  
M. Kumar ◽  
C. W. Bert
Keyword(s):  
Response Characteristic ◽  
Cord Compression ◽  
Complete Characterization ◽  
Strain Response ◽  
Strain Gages ◽  
Experimental Characterization ◽  
Rubber Composites ◽  
Stress Strain ◽  
Strain Data

Abstract Unidirectional cord-rubber specimens in the form of tensile coupons and sandwich beams were used. Using specimens with the cords oriented at 0°, 45°, and 90° to the loading direction and appropriate data reduction, we were able to obtain complete characterization for the in-plane stress-strain response of single-ply, unidirectional cord-rubber composites. All strains were measured by means of liquid mercury strain gages, for which the nonlinear strain response characteristic was obtained by calibration. Stress-strain data were obtained for the cases of both cord tension and cord compression. Materials investigated were aramid-rubber, polyester-rubber, and steel-rubber.

Recent Rapid Climate Changes in Antarctic and their Influence on Low Diversity Ecosystems

2010 ◽  
Vol 17 (1) ◽  
pp. 17-30 ◽  
Author(s):  
Katarzyna J. Chwedorzewska
Keyword(s):  
Environmental Variables ◽  
Climate Changes ◽  
Biological Significance ◽  
Global Changes ◽  
Factors Affecting ◽  
Low Diversity ◽  
Main Factors ◽  
The Antarctic ◽  
High Diversity ◽  
Temperature Water

ABSTRACTThe geographic position, astronomic factors (e.g. the Earth’s maximum distance from the Sun during winter), ice cover and altitude are the main factors affecting the climate of the Antarctic, which is the coldest place on Earth. Parts of Antarctica are facing the most rapid rates of anthropogenic climate change currently seen on the planet. Climate changes are occurring throughout Antarctica, affecting three major groups of environmental variables of considerable biological significance: temperature, water, UV-B radiation.Low diversity ecosystems are expected to be more vulnerable to global changes than high diversity ecosystems

Characterization of CMOS Structures (0.6 µm process) Submitted to HBM and COM ESD Stress Tests

1997 ◽  
Author(s):  
G. Meneghesso ◽  
E. Zanoni ◽  
P. Colombo ◽  
M. Brambilla ◽  
R. Annunziata ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electrostatic Discharge ◽  
Stress Test ◽  
Complete Characterization ◽  
Stress Tests ◽  
Test Procedures ◽  
Emission Microscopy ◽  
Fast Rise ◽  
Scanning Electron

Abstract In this work, we present new results concerning electrostatic discharge (ESD) robustness of 0.6 μm CMOS structures. Devices have been tested according to both HBM and socketed CDM (sCDM) ESD test procedures. Test structures have been submitted to a complete characterization consisting in: 1) measurement of the tum-on time of the protection structures submitted to pulses with very fast rise times; 2) ESD stress test with the HBM and sCDM models; 3) failure analysis based on emission microscopy (EMMI) and Scanning Electron Microscopy (SEM).

scholarly journals Complete characterization of spin chains with two Ising symmetries

2019 ◽  
Vol 125 (1) ◽  
pp. 10008 ◽  
Author(s):  
Bat-el Friedman ◽  
Atanu Rajak ◽  
Emanuele G. Dalla Torre
Keyword(s):  
Complete Characterization ◽  
Spin Chains

scholarly journals On the star forest polytope for trees and cycles

2019 ◽  
Vol 53 (5) ◽  
pp. 1763-1773
Author(s):  
Meziane Aider ◽  
Lamia Aoudia ◽  
Mourad Baïou ◽  
A. Ridha Mahjoub ◽  
Viet Hung Nguyen
Keyword(s):  
Undirected Graph ◽  
Dominating Set ◽  
Discrete Math ◽  
Complete Characterization ◽  
Facial Structure ◽  
Maximum Weight ◽  
Single Node ◽  
End Node ◽  
Vertex Disjoint

Let G = (V, E) be an undirected graph where the edges in E have non-negative weights. A star in G is either a single node of G or a subgraph of G where all the edges share one common end-node. A star forest is a collection of vertex-disjoint stars in G. The weight of a star forest is the sum of the weights of its edges. This paper deals with the problem of finding a Maximum Weight Spanning Star Forest (MWSFP) in G. This problem is NP-hard but can be solved in polynomial time when G is a cactus [Nguyen, Discrete Math. Algorithms App. 7 (2015) 1550018]. In this paper, we present a polyhedral investigation of the MWSFP. More precisely, we study the facial structure of the star forest polytope, denoted by SFP(G), which is the convex hull of the incidence vectors of the star forests of G. First, we prove several basic properties of SFP(G) and propose an integer programming formulation for MWSFP. Then, we give a class of facet-defining inequalities, called M-tree inequalities, for SFP(G). We show that for the case when G is a tree, the M-tree and the nonnegativity inequalities give a complete characterization of SFP(G). Finally, based on the description of the dominating set polytope on cycles given by Bouchakour et al. [Eur. J. Combin. 29 (2008) 652–661], we give a complete linear description of SFP(G) when G is a cycle.

scholarly journals Protein complex formation in methionine chain-elongation and leucine biosynthesis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Li-Qun Chen ◽  
Shweta Chhajed ◽  
Tong Zhang ◽  
Joseph M. Collins ◽  
Qiuying Pang ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Complete Characterization ◽  
Bimolecular Fluorescence Complementation ◽  
Chain Elongation ◽  
Substrate Channeling ◽  
Leucine Biosynthesis ◽  
Protein Complex Formation ◽  
Genes Encoding ◽  
Isopropylmalate Dehydrogenase

AbstractDuring the past two decades, glucosinolate (GLS) metabolic pathways have been under extensive studies because of the importance of the specialized metabolites in plant defense against herbivores and pathogens. The studies have led to a nearly complete characterization of biosynthetic genes in the reference plant Arabidopsis thaliana. Before methionine incorporation into the core structure of aliphatic GLS, it undergoes chain-elongation through an iterative three-step process recruited from leucine biosynthesis. Although enzymes catalyzing each step of the reaction have been characterized, the regulatory mode is largely unknown. In this study, using three independent approaches, yeast two-hybrid (Y2H), coimmunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC), we uncovered the presence of protein complexes consisting of isopropylmalate isomerase (IPMI) and isopropylmalate dehydrogenase (IPMDH). In addition, simultaneous decreases in both IPMI and IPMDH activities in a leuc:ipmdh1 double mutants resulted in aggregated changes of GLS profiles compared to either leuc or ipmdh1 single mutants. Although the biological importance of the formation of IPMI and IPMDH protein complexes has not been documented in any organisms, these complexes may represent a new regulatory mechanism of substrate channeling in GLS and/or leucine biosynthesis. Since genes encoding the two enzymes are widely distributed in eukaryotic and prokaryotic genomes, such complexes may have universal significance in the regulation of leucine biosynthesis.

scholarly journals Genomic Characterization of the Three Balkan Livestock Guardian Dogs

2021 ◽  
Vol 13 (4) ◽  
pp. 2289
Author(s):  
Mateja Janeš ◽  
Minja Zorc ◽  
Maja Ferenčaković ◽  
Ino Curik ◽  
Peter Dovč ◽  
...  
Keyword(s):  
Conservation Management ◽  
Diversity Management ◽  
Genomic Diversity ◽  
Mountainous Areas ◽  
Autosomal Snps ◽  
The Balkans ◽  
Gray Wolves ◽  
Low Diversity ◽  
Genomic Characterization

Balkan Livestock Guardian Dogs (LGD) were bred to help protect sheep flocks in sparsely populated, remote mountainous areas in the Balkans. The aim of this study was genomic characterization (107,403 autosomal SNPs) of the three LGD breeds from the Balkans (Karst Shepherd, Sharplanina Dog, and Tornjak). Our analyses were performed on 44 dogs representing three Balkan LGD breeds, as well as on 79 publicly available genotypes representing eight other LGD breeds, 70 individuals representing seven popular breeds, and 18 gray wolves. The results of multivariate, phylogenetic, clustering (STRUCTURE), and FST differentiation analyses showed that the three Balkan LGD breeds are genetically distinct populations. While the Sharplanina Dog and Tornjak are closely related to other LGD breeds, the Karst Shepherd is a slightly genetically distinct population with estimated influence from German Shepard (Treemix analysis). Estimated genomic diversity was high with low inbreeding in Sharplanina Dog (Ho = 0.315, He = 0.315, and FROH>2Mb = 0.020) and Tornjak (Ho = 0.301, He = 0.301, and FROH>2Mb = 0.033) breeds. Low diversity and high inbreeding were estimated in Karst Shepherds (Ho = 0.241, He = 0.222, and FROH>2Mb = 0.087), indicating the need for proper diversity management. The obtained results will help in the conservation management of Balkan LGD dogs as an essential part of the specific grazing biocultural system and its sustainable maintenance.

scholarly journals Topological Approach to Mathematical Programs with Switching Constraints

2021 ◽  
Author(s):  
Vladimir Shikhman
Keyword(s):  
Stationary Point ◽  
Mountain Pass Theorem ◽  
Strong Stability ◽  
Complete Characterization ◽  
Stationary Points ◽  
Mathematical Programs ◽  
Linear Independence Constraint Qualification ◽  
Point Set ◽  
Stationary Level

AbstractWe study mathematical programs with switching constraints (for short, MPSC) from the topological perspective. Two basic theorems from Morse theory are proved. Outside the W-stationary point set, continuous deformation of lower level sets can be performed. However, when passing a W-stationary level, the topology of the lower level set changes via the attachment of a w-dimensional cell. The dimension w equals the W-index of the nondegenerate W-stationary point. The W-index depends on both the number of negative eigenvalues of the restricted Lagrangian’s Hessian and the number of bi-active switching constraints. As a consequence, we show the mountain pass theorem for MPSC. Additionally, we address the question if the assumption on the nondegeneracy of W-stationary points is too restrictive in the context of MPSC. It turns out that all W-stationary points are generically nondegenerate. Besides, we examine the gap between nondegeneracy and strong stability of W-stationary points. A complete characterization of strong stability for W-stationary points by means of first and second order information of the MPSC defining functions under linear independence constraint qualification is provided. In particular, no bi-active Lagrange multipliers of a strongly stable W-stationary point can vanish.

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