Role of Haptoglobin as a Marker of Muscular Improvement in Patients with Multiple Sclerosis after Administration of Epigallocatechin Gallate and Increase of Beta-Hydroxybutyrate in the Blood: A Pilot Study

Here, we report on the role of haptoglobin (Hp), whose expression depends on the synthesis of interleukin 6 (IL-6), related to the pathogenesis of multiple sclerosis (MS), as a possible marker of muscle improvement achieved after treatment with the polyphenol epigallocatechin gallate (EGCG) and an increase in the ketone body beta-hydroxybutyrate (BHB) in the blood. After 4 months of intervention with 27 MS patients, we observed that Hp does not significantly increase, alongside a significant decrease in IL-6 and a significant increase in muscle percentage. At the same time, Hp synthesis is considerably and positively correlated with IL-6 both before and after treatment; while this correlation occurs significantly reversed with muscle percentage before treatment, no correlation is evident after the intervention. These results seem to indicate that Hp could be a marker of muscle status and could be a diagnosis tool after therapeutic intervention in MS patients.

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Possible Role of Butyrylcholinesterase in Fat Loss and Decreases in Inflammatory Levels in Patients with Multiple Sclerosis after Treatment with Epigallocatechin Gallate and Coconut Oil: A Pilot Study

Nutrients ◽

10.3390/nu13093230 ◽

2021 ◽

Vol 13 (9) ◽

pp. 3230

Author(s):

Jose Enrique de la Rubia Ortí ◽

Jose Luis Platero ◽

Iván Hu Yang ◽

Jose Joaquin Ceron ◽

Asta Tvarijonaviciute ◽

...

Keyword(s):

Multiple Sclerosis ◽

Pilot Study ◽

Body Fat ◽

Ketone Bodies ◽

Intervention Group ◽

Epigallocatechin Gallate ◽

Coconut Oil ◽

Control Group ◽

Fat Percentage

(1) Background. Multiple sclerosis (MS) is characterised by the loss of muscle throughout the course of the disease, which in many cases is accompanied by obesity and related to inflammation. Nonetheless, consuming epigallocatechin gallate (EGCG) and ketone bodies (especially β-hydroxybutyrate (βHB)) produced after metabolising coconut oil, have exhibited anti-inflammatory effects and a decrease in body fat. In addition, butyrylcholinesterase (BuChE), seems to be related to the pathogenesis of the disease associated with inflammation, and serum concentrations have been related to lipid metabolism. Objective. The aim of the study was to determine the role of BuChE in the changes caused after treatment with EGCG and ketone bodies on the levels of body fat and inflammation state in MS patients. (2) Methods. A pilot study was conducted for 4 months with 51 MS patients who were randomly divided into an intervention group and a control group. The intervention group received 800 mg of EGCG and 60 mL of coconut oil, and the control group was prescribed a placebo. Fat percentage and concentrations of the butyrylcholinesterase enzyme (BuChE), paraoxonase 1 (PON1) activity, triglycerides, interleukin 6 (IL-6), albumin and βHB in serum were measured. (3) Results. The intervention group exhibited significant decreases in IL-6 and fat percentage and significant increases in BuChE, βHB, PON1, albumin and functional capacity (determined by the Expanded Disability Status Scale (EDSS)). On the other hand, the control group only exhibited a decrease in IL-6. After the intervention, BuChE was positively correlated with the activity of PON1, fat percentage and triglycerides in the intervention group, whereas these correlations were not observed in the control group (4). Conclusions. BuChE seems to have an important role in lipolytic activity and the inflammation state in MS patients, evidenced after administering EGCG and coconut oil as a βHB source.

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A Pilot Study to Explore the Role of Gut Flora in Multiple Sclerosis

Case Medical Research ◽

10.31525/ct1-nct04148313 ◽

2019 ◽

Author(s):

Keyword(s):

Multiple Sclerosis ◽

Pilot Study ◽

Gut Flora

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Satiating Effect of a Ketogenic Diet and Its Impact on Muscle Improvement and Oxidation State in Multiple Sclerosis Patients

Nutrients ◽

10.3390/nu11051156 ◽

2019 ◽

Vol 11 (5) ◽

pp. 1156 ◽

Cited By ~ 9

Author(s):

María Benlloch ◽

María Mar López-Rodríguez ◽

María Cuerda-Ballester ◽

Eraci Drehmer ◽

Sandra Carrera ◽

...

Keyword(s):

Multiple Sclerosis ◽

Body Composition ◽

Ketogenic Diet ◽

Oxidation State ◽

Lean Mass ◽

Ketone Bodies ◽

Paraoxonase 1 ◽

Before And After ◽

Multiple Sclerosis Patients ◽

Beta Hydroxybutyrate

Background: It was previously established that Multiple sclerosis (MS) generates energy alterations at the mitochondrial level related to the loss of muscle mass. Ketone bodies, mainly beta-hydroxybutyrate (BHB), re-establish this energy alteration causing satiety, changes in body composition and a decrease in hormone-dependant hunger, such as ghrelin. The aim of this study was to establish possible improvements in body composition and the level of oxidation in patients with MS, by means of the satiating effect of a ketogenic diet. Methods: A pilot study was carried out with 27 MS patients who were given a Mediterranean isocaloric and ketogenic diet for 4 months. Anthropometric measurements, as well as satiety and hunger perception (VAS scale), were taken. In addition, BHB and paraoxonase 1 (PON1), as an oxidation marker, were measured by spectrophotometric automated assays, and ghrelin was determined by an enzyme immunoassay in the serum. All measurements were taken before and after the intervention. Results: A significant increase in satiety perception at lunch and dinner and of BHB in the blood was obtained. Hunger perception decreased significantly at lunch and dinner with similar levels of ghrelin. In addition, an important increase in lean mass and PON1 was observed. To our knowledge, this is the first study addressing improvements in body composition, oxidation state and metabolism in MS patients, based on the satiating effect of a Mediterranean isocaloric diet. Conclusion: A ketogenic diet increases lean mass and decreases inflammation and oxidation possibly as a consequence of an increase in satiety and decrease in hunger in MS patients.

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P1.04-64 The Opposite Role of PD-L1 Expression in EGFR Mutant Lung Cancer Treated with PD-1 Inhibitor Before and After EGFR TKI: Pilot Study

Journal of Thoracic Oncology ◽

10.1016/j.jtho.2019.08.967 ◽

2019 ◽

Vol 14 (10) ◽

pp. S466

Author(s):

H.S. Park ◽

S.S. Cho ◽

H.J. An ◽

J. Yoo ◽

B.Y. Shim ◽

...

Keyword(s):

Lung Cancer ◽

Pilot Study ◽

Before And After ◽

Egfr Mutant ◽

Egfr Tki

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ROLE OF UDUMBARADI TAILA YONIPICHU IN THE MANAGEMENT OF KAPHAJA YONIYVAPAD - A PILOT STUDY

November 2020 - International Ayurvedic Medical Journal ◽

10.46607/iamj0708112020 ◽

2020 ◽

Vol 08 (11) ◽

pp. 4986-4994

Author(s):

Sonali R Gaikwad ◽

Shreyes. S ◽

Yogitha Bali M. R

Keyword(s):

Pilot Study ◽

Reproductive System ◽

Vaginal Discharge ◽

Physiological Condition ◽

Female Reproductive System ◽

Female Patients ◽

Medical College ◽

Before And After ◽

Single Blind

Background: Vaginal discharge, commonly known as leucorrhea, is a physiological condition which happens in female reproductive system. More than 75% women experience leucorrhea during their lifetime and 45% of them has recurrence. Objectives: To assess the efficacy of Udumbaradi Taila Yoni Pichu in management of Kaphaja Yoni Vyapad. Design: This is a single blind pilot study. 20 female patients with the complaints of Kaphaja Yonivyapad (nonspecific leucorrhoea) from the Dept. of Prasooti tantra and Stree Roga OPD of Rajiv Gandhi educational society’s Ayurvedic Medical College, Ron were included for the study. Patients were administered Udumbaradi Taila Yonipichu for 7 days and were assessed before and after the treatment and followed up on the 14th day. Results: This pilot study showed statistically significant changes with Udumbaradi Taila Yonipichu in reducing the complaints of Kaphaja Yoni Vyapad such as Katishoola (p<0.001), Kandu (p<0.001), consistency of the Srava (p<0.001) and Srava Pramana (quantity of the discharge) (p<0.001). Conclusions: Udumbaradi Taila Yonipichu showed effectiveness in the management of Kaphaja yoni Vyapad with the reduction of Katishoola, Kandu, consistency of the Srava and Srava pramana after the treatment.

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Effective Rehabilitation Methods in Patients with Multiple Sclerosis

US Neurology ◽

10.17925/usn.2010.05.02.67 ◽

2010 ◽

Vol 05 (02) ◽

pp. 67 ◽

Cited By ~ 3

Author(s):

Susan E Bennett ◽

Keyword(s):

Multiple Sclerosis ◽

Functional Recovery ◽

Therapeutic Intervention ◽

Aerobic Training ◽

Major Focus ◽

Pharmacological Management ◽

Comprehensive Management ◽

The Past ◽

Moderate Disease

The role of rehabilitation in managing symptoms of multiple sclerosis (MS) and promoting functional recovery has grown exponentially over the past 12 years. Evidence now supports strength and aerobic training for individuals with mild or moderate disease. An emerging body of evidence suggests that exercise may play a role in the production of brain-derived neurotrophic factor in individuals with MS, and the combination of pharmacological management and rehabilitation may produce better outcomes than one therapeutic intervention alone. Rehabilitation and exercise should be a major focus in the comprehensive management of MS.

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GRAPPA 2019 Research Recipient Awards Report

The Journal of Rheumatology ◽

10.3899/jrheum.201682 ◽

2021 ◽

pp. jrheum.201682

Author(s):

Arthur Kavanaugh ◽

Alla Ishchenko ◽

Rik J. Lories ◽

Kurt de Vlam ◽

Zhenrui Shi ◽

...

Keyword(s):

Psoriatic Arthritis ◽

Pilot Study ◽

Disease Severity ◽

Therapeutic Intervention ◽

Prospective Pilot Study ◽

Human Joints

A summary of the research conducted by the recipients of the 2019 Group for Research and Assessment of Psoriasis and Psoriatic Arthritis (GRAPPA) Research Awards is presented. Dr. Alla Ishchenko’s project was “Role of Metabolomics in Diagnosis, Disease Severity, and Progression in Psoriasis and Psoriatic Arthritis: A 2-year Prospective Pilot Study” and Dr. Zhenrui Shi’s project was “Preclinical Analysis of CCR6 and CCL20 in Mouse and Human Joints, Respectively, as Targets of Therapeutic Intervention in Psoriatic Arthritis.”

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A pilot study into ecological burning in forests as part of a species conservation plan

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220328 ◽

2021 ◽

Vol 22 (3) ◽

Cited By ~ 1

Author(s):

Matthew W Pearson

Keyword(s):

Pilot Study ◽

Fire Management ◽

Soil Seed Bank ◽

Species Conservation ◽

Management Plan ◽

Diverse Range ◽

Before And After ◽

Conservation Plan ◽

Recruitment Tool

Abstract. Pearson MW. 2021. A pilot study into ecological burning in forests as part of a species conservation plan. Biodiversitas 22: 1296-1303. Fire management and the process of managing communities has become an increasingly topical subject due to recent events. The role of fire for fuel reduction with areas set aside for conservation may not be compatible as a seed recruitment tool for Allocasuarina robusta. Presently, fire as a recruitment tool on A. robusta is not well understood. The pilot study aims to examine the latent seedbank ability to contribute to the conservation of A. robusta when ecological burning is part of a fire management plan. The investigation examined the soil seed bank beneath A. robusta before and after an ecological burn as a greenhouse experiment. The experiment results demonstrated that a diverse range of species responds to a fire that may out-compete the seed recruitment from A. robusta. The results indicate that A. robusta may not be recruiting through seed. Competition appears to be a factor that may limit the ability of A. robusta to recruit from seed.

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The Impact of Epigallocatechin Gallate and Coconut Oil Treatment on Cortisol Activity and Depression in Multiple Sclerosis Patients

Life ◽

10.3390/life11040353 ◽

2021 ◽

Vol 11 (4) ◽

pp. 353

Author(s):

Jose Luis Platero ◽

María Cuerda-Ballester ◽

David Sancho-Cantus ◽

María Benlloch ◽

Jose Joaquin Ceron ◽

...

Keyword(s):

Multiple Sclerosis ◽

Body Fat ◽

Intervention Group ◽

Epigallocatechin Gallate ◽

Coconut Oil ◽

Control Group ◽

Mass Percentage ◽

Fat Loss ◽

Before And After ◽

The Impact

(1) Background: Multiple sclerosis (MS) is pathogenically characterized by high oxidative stress and symptomatically by progressive muscle loss and increased body fat associated with the presence of depression. Epigallocatechin gallate (EGCG) (particularly present in green tea) and ketone bodies (in particular beta-hydroxybutyrate (BHB)), whose main source is coconut oil, have shown emotional benefits and body fat loss. The aim of this study was to assess the impact of EGCG and coconut oil on cortisol activity related to fat loss and depression in MS patients. (2) Methods: The study involved 51 MS patients who were randomly divided into an intervention group or a control group. The intervention group received 800 mg of EGCG and 60 mL of coconut oil, which were included in their daily diet for four months. The control group received placebo and all patients followed an isocaloric diet. A blood sample was collected before and after the four-month period, and levels of cortisol, albumin and BHB were measured in serum. In addition, immediately before and after the intervention, anthropometric variables were measured: waist-to-hip ratio (WHR), body fat mass percentage, fat weight, total weight, and muscle mass percentage. Depression was assessed with the Beck Depression Inventory II (BDI-II). (3) Results: No significant changes were obtained in cortisol levels in any of the groups, and there was a significant increase in albumin in the blood of the intervention group only that could lead to a decrease in serum free cortisol. In addition, it was observed a significant decrease in levels of depression and abdominal fat. (4) Conclusions: EGCG combined with coconut oil increase the concentration of albumin in blood and produce less depression in MS patients.

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Role of Phala Ghruta Uttarabasti in the management of female infertility w.s.r to follicular study

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.5.4.8 ◽

2020 ◽

Vol 5 (04) ◽

pp. 45-49

Author(s):

Suvarna P. Nidagundi ◽

Savita S. Patil

Keyword(s):

Pilot Study ◽

Menstrual Cycle ◽

Psychological Factors ◽

Sedentary Lifestyle ◽

Signs And Symptoms ◽

Female Infertility ◽

Fertile Period ◽

Anovulatory Cycle ◽

Before And After

Background: Streevandhyatva or Female Infertility is a Gynaecological disorder. The etiological factors include the defect in the immature Sperm and Ovum during the fertile period. Incidence rate according to W.H.O is 20-30% is primary while 70-80% of cases are secondary Infertility. Now a day’s maximum number of Female Infertility is due to sedentary lifestyle, Psychological factors, Nutritional deficiency and Stress leads to different condition like PCOD, Obesity, Irregular Menstrual cycle and anovulatory cycle etc. The present study was carried out as a pilot study on 10 subjects of female infertility with the use of Phalagruta Uttarabasti followed by Prakshalana and Pichudharana. Three to five consecutive cycles of Phalagruta Uttarabasti was administered, Posology - Uttarabasti with Phalagruta 5ml daily for 3 days. Diagnostic criteria - Patients are diagnosed based on signs and symptoms like anovulation, PCOD etc., before and after treatment with USG. Results - Among 10 patients, 4 patients - Well response, 2 patients - Improved with follicular study, 3 patients - Poor responded, 1 Patient discontinued the study.

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