Health Aspects, Growth Performance, and Meat Quality of Rabbits Receiving Diets Supplemented with Lettuce Fertilized with Whey Protein Hydrolysate Substituting Nitrate

Lettuce (Lactuca sativa) was grown using a foliar spray with whey protein hydrolysate (WPH) as opposed to normal nitrate fertilization. Lettuce juice was prepared from lettuce cultivated without any fertilization, nitrate fertilization, or WPH. Sixty weaned, 4-week-old male V-line rabbits with an average 455 ± 6 g body weight were randomly divided into 4 groups (n = 15) and administered different lettuce juices. Rabbits administered WPH-fertilized lettuce showed significantly higher (n = 5, p < 0.05) body weight and carcass weight than those receiving nitrate-fertilized lettuce. Rabbits administered nitrate-fertilized lettuce were associated with significantly (p < 0.05) higher levels of liver enzyme activities (AST, ALT, and ALP), bilirubin (total, direct, and indirect), and kidney biomarkers (creatinine, urea, and uric acid). Rabbits administered WPH-fertilized lettuce avoided such increases and exhibited normal levels of serum proteins. Rabbits administered nitrate-fertilized lettuce manifested significantly (p < 0.05) lower RBCs and Hb levels than that of the other groups, while those receiving WPH-fertilized lettuce showed the highest levels. Liver and kidney sections of rabbits receiving WPH-fertilized lettuce witnessed the absence of the histopathological changes induced by feeding on nitrate-fertilized lettuce and produced higher quality meat. WPH-lettuce can substitute nitrate-fertilized lettuce in feeding rabbits for better performance and health aspects.

Download Full-text

Preparation and characterization of whey protein hydrolysate-Zn complexes

Journal of Food Measurement & Characterization ◽

10.1007/s11694-019-00287-1 ◽

2019 ◽

Vol 14 (1) ◽

pp. 254-261 ◽

Cited By ~ 2

Author(s):

Rongchun Wang ◽

Shenghua He ◽

Yifan Xuan ◽

Cuilin Cheng

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Whey Protein Hydrolysate

Download Full-text

Correction: Whey Protein Hydrolysate Enhances HSP90 but Does Not Alter HSP60 and HSP25 in Skeletal Muscle of Rats

PLoS ONE ◽

10.1371/annotation/0cb4a83a-2b26-410f-bdda-ff62dae25eea ◽

2014 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Carolina Soares Moura ◽

Pablo Christiano Barboza Lollo ◽

Priscila Neder Morato ◽

Luciana Hisayama Nisishima ◽

Everardo MagalhÃ£es Carneiro ◽

...

Keyword(s):

Skeletal Muscle ◽

Whey Protein ◽

Protein Hydrolysate ◽

Whey Protein Hydrolysate

Download Full-text

IMPACT OF FORTIFYING COW'S MILK WITH WHEY PROTEIN HYDROLYSATE ON YOGHURT QUALITY

Menoufia Journal of Food and Dairy Sciences ◽

10.21608/mjfds.2020.171450 ◽

2020 ◽

Vol 5 (5) ◽

pp. 65-77

Author(s):

K. M. K. Kebary ◽

S. A. Husien ◽

R. M. Badawi ◽

M. A. M. Habib

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Cow’S Milk ◽

Cow's Milk ◽

Whey Protein Hydrolysate

Download Full-text

Co-ingestion of whey protein hydrolysate with milk minerals rich in calcium potently stimulates glucagon-like peptide-1 secretion: an RCT in healthy adults

European Journal of Nutrition ◽

10.1007/s00394-019-02092-4 ◽

2019 ◽

Vol 59 (6) ◽

pp. 2449-2462 ◽

Cited By ~ 1

Author(s):

Yung-Chih Chen ◽

Harry A. Smith ◽

Aaron Hengist ◽

Oliver J. Chrzanowski-Smith ◽

Ulla Ramer Mikkelsen ◽

...

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Healthy Adults ◽

Mean Difference ◽

High Dose ◽

Calcium Citrate ◽

Double Blind ◽

Gut Hormone ◽

Whey Protein Hydrolysate ◽

Milk Minerals

Abstract Purpose To examine whether calcium type and co-ingestion with protein alter gut hormone availability. Methods Healthy adults aged 26 ± 7 years (mean ± SD) completed three randomized, double-blind, crossover studies. In all studies, arterialized blood was sampled postprandially over 120 min to determine GLP-1, GIP and PYY responses, alongside appetite ratings, energy expenditure and blood pressure. In study 1 (n = 20), three treatments matched for total calcium content (1058 mg) were compared: calcium citrate (CALCITR); milk minerals rich in calcium (MILK MINERALS); and milk minerals rich in calcium plus co-ingestion of 50 g whey protein hydrolysate (MILK MINERALS + PROTEIN). In study 2 (n = 6), 50 g whey protein hydrolysate (PROTEIN) was compared to MILK MINERALS + PROTEIN. In study 3 (n = 6), MILK MINERALS was compared to the vehicle of ingestion (water plus sucralose; CONTROL). Results MILK MINERALS + PROTEIN increased GLP-1 incremental area under the curve (iAUC) by ~ ninefold (43.7 ± 11.1 pmol L−1 120 min; p < 0.001) versus both CALCITR and MILK MINERALS, with no difference detected between CALCITR (6.6 ± 3.7 pmol L−1 120 min) and MILK MINERALS (5.3 ± 3.5 pmol L−1 120 min; p > 0.999). MILK MINERALS + PROTEIN produced a GLP-1 iAUC ~ 25% greater than PROTEIN (p = 0.024; mean difference: 9.1 ± 6.9 pmol L−1 120 min), whereas the difference between MILK MINERALS versus CONTROL was small and non-significant (p = 0.098; mean difference: 4.2 ± 5.1 pmol L−1 120 min). Conclusions When ingested alone, milk minerals rich in calcium do not increase GLP-1 secretion compared to calcium citrate. Co-ingesting high-dose whey protein hydrolysate with milk minerals rich in calcium increases postprandial GLP-1 concentrations to some of the highest physiological levels ever reported. Registered at ClinicalTrials.gov: NCT03232034, NCT03370484, NCT03370497.

Download Full-text

Prophylactic effect of vitamin E against hepatotoxicity, nephrotoxicity, haematological indices and histopathology induced by diazinon insecticide in mice

Current Zoology ◽

10.1093/czoolo/55.3.219 ◽

2009 ◽

Vol 55 (3) ◽

pp. 219-226 ◽

Cited By ~ 11

Author(s):

Nahla S. El-Shenawy ◽

Rasha A. Al-Eisa ◽

Fawzia El-Salmy ◽

Omema Salah

Keyword(s):

Body Weight ◽

Vitamin E ◽

Kidney Function ◽

Kidney Tissue ◽

The Body ◽

High Dose ◽

Protective Effects ◽

Histopathological Changes ◽

Liver And Kidney ◽

Haematological Indices

Abstract Considering that the involvement of reactive oxygen species (ROS) has been implicated in the toxicity of various pesticides, this study was designed to study the ameliorative effect of Vitamin E (100 mg/kg body weight) on mice (25 - 30 mg) treated with diazinon (32.5 or 16.25 mg/kg body weight) organophosphate insecticide for 14 days. Subchronic DZN exposure and the protective effects of vitamins E (vitE) were evaluated for their effects on haematological indices, the enzymes concerning liver damage [plasma alanine aminotransferase (ALT), aspartate aminotaransferase (AST), alkaline phosphatise (AIP), and some parameters of kidney function (urea and creatinine) in mice. Additionally, the histopathological changes in liver and kidney tissue were examined. The high dose of diazinon (DZNH) decreased the body weight significantly at the end of experiment. Additionally, the liver and kidney were examines for histopathological changes. The high dose of diazinon decreased body weight significantly. Moreover, there was a statistically significant decrease in haemoglobin (Hb), red blood cell (RBC) and hematocrit (Hct) in diazinon-treated mice compared to controls. This decrease was partially remedied in the diazinon-treated group that also received vitE. Damage in the liver and kidney tissues was also evident as elevated plasma ALT, AST, ALP, urea and creatinine. VitE partially counteracts the toxic effect of DZN and repairs tissue damage in the liver and kidney, especially when supplemented to 1/4 LD50 intoxicated animals. Histopathological changes in liver and kidney were observed only in 32.5 mg/kg DZN given group. These results suggest that the effects of DZN are dose dependent. No pathological findings were observed in vitE + DZN treated groups. According to the present study, we conclude that vitE can reduce the detrimental impacts of diazinon on haematological indicies, as well as liver and kidney function.

Download Full-text

Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice

Nutrients ◽

10.3390/nu12113362 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3362

Author(s):

Ji Eun Shin ◽

Seok Jun Park ◽

Seung Il Ahn ◽

Se-Young Choung

Keyword(s):

Skeletal Muscle ◽

Whey Protein ◽

Muscle Mass ◽

Muscle Atrophy ◽

Skeletal Muscle Mass ◽

Protein Hydrolysate ◽

Protein Hydrolysates ◽

Protein Supplementation ◽

Whey Protein Hydrolysates ◽

Whey Protein Hydrolysate

Sarcopenia, a loss of skeletal muscle mass and function, is prevalent in older people and associated with functional decline and mortality. Protein supplementation is necessary to maintain skeletal muscle mass and whey protein hydrolysates have the best nutrient quality among food proteins. In the first study, C57BL/6 mice were subjected to immobilization for 1 week to induce muscle atrophy. Then, mice were administered with four different whey protein hydrolysates for 2 weeks with continuous immobilization. Among them, soluble whey protein hydrolysate (WP-S) had the greatest increase in grip strength, muscle weight, and cross-sectional area of muscle fiber than other whey protein hydrolysates. To investigate the molecular mechanism, we conducted another experiment with the same experimental design. WP-S significantly promoted the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway and inhibited the PI3K/Akt/forkhead box O (FoxO) pathway. In addition, it increased myosin heavy chain (MyHC) expression in both the soleus and quadriceps and changed MyHC isoform expressions. In conclusion, WP-S attenuated muscle atrophy induced by immobilization by enhancing the net protein content regulating muscle protein synthesis and degradation. Thus, it is a necessary and probable candidate for developing functional food to prevent sarcopenia.

Download Full-text