scholarly journals Complementary Effect of Non-Persistent Silver Nano-Architectures and Chlorhexidine on Infected Wound Healing

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1215
Author(s):  
Mykola Pernakov ◽  
Maria Laura Ermini ◽  
Oksana Sulaieva ◽  
Domenico Cassano ◽  
Marco Santucci ◽  
...  
Keyword(s):  
Wound Healing ◽  
Infected Wound ◽  
Bacterial Strains ◽  
Efficacy Evaluation ◽  
Complementary Effect ◽  
Macrophages Polarization ◽  
Bacteriological Profile ◽  
Purulent Wound ◽  
The Common

Surgical site infection (SSI) substantially contributes each year to patients’ morbidity and mortality, accounting for about 15% of all nosocomial infections. SSI drastically increases the rehab stint and expenses while jeopardizing health outcomes. Besides prevention, the treatment regime relies on an adequate antibiotic therapy. On the other hand, resistant bacterial strains have currently reached up to 34.3% of the total infections, and this percentage grows annually, reducing the efficacy of the common treatment schemes. Thus, new antibacterial strategies are urgently demanded. Here, we demonstrated in rats the effectiveness of non-persistent silver nano-architectures (AgNAs) in infected wound healing together with their synergistic action in combination with chlorhexidine. Besides the in vivo efficacy evaluation, we performed analysis of the bacteriological profile of purulent wound, histological evaluations, and macrophages polarization quantifications to further validate our findings and elucidate the possible mechanisms of AgNAs action on wound healing. These findings open the way for the composition of robust multifunctional nanoplatforms for the translation of safe and efficient topical treatments of SSI.

Gold nanoplates with superb photothermal efficiency and peroxidase-like activity for rapid and synergistic antibacterial therapy

2021 ◽  
Author(s):  
Lu Yan ◽  
Jie Mu ◽  
Pengxin Ma ◽  
Qian Li ◽  
Pengxue Yin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conversion Efficiency ◽  
Antibacterial Therapy ◽  
Infected Wound ◽  
Photothermal Conversion ◽  
Antibacterial Ability

Gold nanoplates exhibit 68.5% photothermal conversion efficiency and peroxidase-like activity, and AuNPTs (50 μg mL−1)/H2O2 (0.1 mM)/NIR (1 W cm−2, 3 min) show excellent synergistic antibacterial ability and promote MRSA-infected wound healing in vivo.

scholarly journals MORINGA OLEIFERA LEAF EXTRACT LOADED HYDROGEL FOR DIABETIC WOUND HEALING

2018 ◽  
Vol 2 (2) ◽  
pp. 35-41
Author(s):  
Satheesh Babu Natarajan ◽  
Sreemoy Kanti Das ◽  
Suriyakala Perumal Chandran ◽  
Aung Myo Oo ◽  
Sowmya Shar Kanneppady ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Irritation ◽  
World Health ◽  
Albino Rats ◽  
Diabetic Patients ◽  
Bacterial Strains ◽  
Anticancer Activities ◽  
Medicinal Uses ◽  
Wound Induction

Diabetic wounds (DW) are a chronic, non-healing wound on the feet of diabetic patients that pose a serious challenge to world health. Around 84% of diabetic patients undergo lower leg amputations. Though numerous topical and systemic drugs have been used to heal the DW, these drugs have led to the emergence and subsequent rapid overgrowth of resistant bacterial strains, side effects and toxicity. Many herbal plants have very important role in wound healing because they promote the natural repair mechanisms. Moringaoleifera (MO) is an important medicinal plant which has an impressive range of medicinal uses including antimicrobial, anti-inflammatory, antidiabetic, antioxidant and anticancer activities. Recently few researchers reported that MO extracts have effective wound healing property due to the presence of rich flavonoids and vicenin-2. The objective of the present study was to develop hydrogel formulations loaded with Moringaoleifera leaves extract. The prepared hydrogels were evaluated for physical appearance, rheological behavior, skin irritation and wound-healing power in streptozotocin-induced diabetic male wistar albino rats. Results showed that all hydrogel formulations exhibited good and acceptable physical properties. All the animals tolerated the applied gels and no signs of irritations were noticed during the skin irritation study. The in-vivo wound healing studies showed a time dependent increase in percentage of wound, a contraction which is higher than that produced by the control groups. These contractions were statistically significant (P<0.001), during the first 10 days of the study with MO-Hydrogel administration. The MO-hydrogel showed the highest percent wound contraction with complete wound closure and epithelization was observed on 7thday of wound induction.

scholarly journals Metal nanoparticles and biomaterials: The multipronged approach for potential diabetic wound therapy

2021 ◽  
Vol 10 (1) ◽  
pp. 653-670
Author(s):  
Ahmad Yasser Hamdi Nor Azlan ◽  
Haliza Katas ◽  
Mohd Fauzi Mh Busra ◽  
Nur Atiqah Mohamad Salleh ◽  
Ali Smandri
Keyword(s):  
Wound Healing ◽  
Metal Nanoparticles ◽  
Acinetobacter Calcoaceticus ◽  
Dual Therapy ◽  
Diabetic Wound ◽  
Bacterial Strains ◽  
Barriers And Challenges ◽  
Diabetic Wounds

Abstract Metal nanoparticles have been widely used in the treatment of diabetic wounds owing to their proven antibacterial activity and enhanced wound healing effects. Therefore, in this review, we discuss the use of metal nanoparticles in managing diabetic wounds, mainly silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), and zinc nanoparticles (ZnO nanoparticles), as well as their combination with biomaterials such as chitosan, bacterial cellulose, growth factors, etc. The combination of metal nanoparticles and biomaterials reportedly halts the growth and multiplication of bacterial strains commonly involved in diabetic wounds, including gram-positive (Staphylococcus aureus and Acinetobacter calcoaceticus) and gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae). Furthermore, these combinations have demonstrated enhanced wound healing of diabetic wounds during in vitro and in vivo studies. Additionally, we highlighted the barriers and challenges associated with the use of metal nanoparticles, including toxicities. Moreover, toxicities were mainly related to the method of synthesis employed, as well as the physical characteristics of nanoparticles, including size, shape, surface charge, and morphology. Collectively, dual-therapy composed of metal nanoparticles and biomaterials has been shown to promote wound healing and can be developed as a promising future therapy for better outcomes in diabetic wound healing.

scholarly journals Adjuvant Antibiotic Activity of Acidic Sophorolipids with Potential for Facilitating Wound Healing

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Helen L. Lydon ◽  
Niki Baccile ◽  
Breedge Callaghan ◽  
Roger Marchant ◽  
Christopher A. Mitchell ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Wound Healing ◽  
Time Course ◽  
Healing Process ◽  
Mouse Skin ◽  
Pharmaceutical Products ◽  
Bacterial Strains ◽  
Content Type

ABSTRACT The sophorolipid class of biosurfactants is finding increasing use in personal care as well as pharmaceutical products and has the potential to disrupt biofilm formation and inhibit the growth of a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from nonpathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded nonacetylated acidic C18:1 congeners that were essentially free from other contaminants (>95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents Enterococcus faecalis and Pseudomonas aeruginosa, with significant reductions in CFU at concentrations of as low as 5 mg ml−1. In addition, the sophorolipid showed similar effects against the same two bacterial strains when combined with kanamycin or cefotaxime. As a potential use of these sophorolipids is as a component of topically applied creams for the treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this, we evaluated mammalian cell toxicity in vitro using viability tests, which revealed no adverse effect on either endothelial or keratinocyte-derived cell lines with sophorolipid concentrations of < 0.5 mg ml−1. In addition, in vivo experiments using a mouse skin wounding assay revealed that the time course of healing wounds was unaffected by the application of sophorolipid-containing creams, and histological examination of regenerated skin tissue confirmed that the healing process was similar to that observed for control animals, with no evidence of inflammation. These results are consistent with the suggestion that acidic sophorolipids can be used as a component of antimicrobial creams to reduce the risk of wound infection during healing.

scholarly journals In Vivo Assessments of Minocycline combined Hyaluronic Acid-mediated Ultrasound Therapy of Infected Wound in Rats

2018 ◽  
Author(s):  
Hongbo Zhang ◽  
Deshu Zhuang
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Visual Inspection ◽  
Control Group ◽  
Ultrasound Therapy ◽  
Infected Wound ◽  
Hematoxylin And Eosin ◽  
Skin Scar

AbstractObjectivesThe purpose of our research was to examine the effects of Minocycline combined hyaluronic acid (HA)-mediated Ultrasound therapy of infected wound in wister rats.Methods40 female wister rats were made wound on the two side of the backbone, then infected in Staphylococcus aureus at the comic for three times. then, they are divided into four groups: control group, minocycline combined HA alone, ultasound alone, minocycline combined HA-mediated ultasound group, respective. After 3 times of treatments, the rats were killed and made into specimens. Assessments consisted of visual inspection in the change of the skin, scar formation pathological morphology by hematoxylin and eosin(HE) stain with optical microscopy, IL-1B assaying and TNF-a were performed.ResultCompared with control group, minocycline combined HA alone, ultasound alone, minocycline combined HA-mediated ultasound group all have effect for wound healing, there was a obvious improvement in all parameters over the duration of the experiment(P<0.05). Compared with the control group, minocycline combined HA-mediated ultasound group indicated less inflammation cells (P<0.001) and the reduce of and IL-1B and TNF-a (P<0.001).ConclusionMinocycline combined HA-mediated ultrasound can accelerate tissue regrowth, which exert significant benefits in healing the wounds.

Novel antimicrobial ointment for infected wound healing in an in vitro and in vivo porcine model

2021 ◽  
Author(s):  
Ray Hachem ◽  
Umang M. Parikh ◽  
Ruth Reitzel ◽  
Joel Rosenblatt ◽  
Aditya Kaul ◽  
...  
Keyword(s):  
Wound Healing ◽  
Porcine Model ◽  
Infected Wound

scholarly journals Evaluation of Antibacterial and In vivo Wound healing activity of Carbon Dot Nanoparticles

2019 ◽  
Vol 2 (3) ◽  
pp. 11-23 ◽  
Author(s):  
Muktha H ◽  
Sharath R ◽  
Nagaraju Kottam ◽  
Srinath S ◽  
Randhir Y
Keyword(s):  
Wound Healing ◽  
Medical Science ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Carbon Dot ◽  
Bacteriostatic Agent ◽  
Potential Applications ◽  
And Control ◽  
Wound Healing Activity

Treating post-surgical wound is one of the major challenges in the field of medical Science due to the several disadvantages posed by the antibiotics. The antimicrobial and Wound healing activity of metal based nanoparticles were well known, but the effects of carbon dot nanoparticles (C-Dots) are less explored. In the present study a wet chemical method for the synthesis of C-Dots from sucrose, glucose and fructose was developed and they were characterized by UV-Visible, Fluorescent spectroscopic techniques and the pharmaceutical applications such as antibacterial and the wound healing activities were evaluated. The study revealed that the C-Dots synthesized from Sucrose (SCD) and Glucose (GCD) showed potent antibacterial activity against both positive and negative bacterial strains at 12.5µl/ml (0.1 ± 0.003) and Fructose (FCD) at 50 µl/ml (0.1 ± 0.01) compared to the vehicle Control (0.61 ± 0.06). The wound healing of SCD (367.8 ± 15.2) was observed better than the Control (280.8 ± 10.72) and FCD (326.8 ± 9.41). GCD (166.8 ± 10.83) skin tissues indicated best healing as compared to FCD (135.8 ± 8.29), SCD (157.3 ± 16.97) and Control (135.8 ± 8.29). The results suggest that C-Dots applied topically possess wound healing activity and have potential applications as a bacteriostatic agent.

Dental Pulp Mesenchymal Stem Cells Promote Polarization of M2 Macrophages and Accelerate Wound Healing by Secreting CCL2

2021 ◽  
Author(s):  
Zi Yang ◽  
Linsha Ma ◽  
Congling Du ◽  
Jinsong Wang ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
M2 Macrophages ◽  
Cell Sheets ◽  
Regeneration Potential ◽  
Macrophages Polarization

Abstract Background Mesenchymal stem cells (MSCs) are widely used in tissue engineering owing to their regenerative potential and immunomodulatory capacity. The crosstalk between MSCs and the host immune function plays a key role in the efficiency of tissue regeneration. However, the difference in immunological modulation and tissue regeneration function between MSCs from different sources remains unclear. Methods Human mesenchymal stem cells derived from bone marrow (BMMSCs), periodontal ligament (PDLSCs), adipose (ADSCs), and dental pulp (DPSCs) were obtained and induced to form cell sheets under the condition of 20 ug/ml vitamin C. The MSC cell sheets carried by hydroxyapatite/tricalciumphosphate (HA/TCP) particles were transplanted subcutaneously into C57BL6 mice for 8 weeks. Histological analyses were performed to detect the tissue regeneration potential and macrophages polarization in vivo. Then, THP-1 macrophages were co-cultured with MSCs and quantitative real-time polymerase chain reaction, immunofluorescent staining, western blotting, and enzyme-linked immunosorbent assay were used to investigate the function and mechanism of MSCs on macrophages in vitro. Finally, a wound healing model of the palatal mucosa was performed to confirm the effect of MSCs on macrophages and tissue healing efficiency. Results Compared to PDLSCs, BMMSCs, and ADSCs, DPSCs exhibited greater tissue regeneration potential, with greater tissue volume, higher Ki67 expression, and less apoptosis in the regenerated tissue of wild-type C57BL6 mice. In addition, DSPCs triggered more M2 macrophages in the regenerated tissue than other MSCs. Our data showed that DPSCs exhibited higher expression levels of C-C Motif Chemokine Ligand 2 (CCL2), and specific blocking of CCL2 by neutralising antibodies can significantly inhibit the DPSCs-induced polarization of M2 macrophages. Finally, DPSCs transplantation promoted wound healing of the palatal mucosa and M2 macrophages polarization in vivo, which could be significantly impaired by CCL2 neutralising antibody. Conclusions Our data indicate that DPSCs exert better tissue regeneration potential and immunoregulatory function by secreting CCL2. These results suggest that CCL2 application can enhance MSC-mediated tissue regeneration or wound healing.

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