Quantitative Imaging Parameters of Contrast-Enhanced Micro-Computed Tomography Correlate with Angiogenesis and Necrosis in a Subcutaneous C6 Glioma Model

The aim of this work was to systematically obtain quantitative imaging parameters with static and dynamic contrast-enhanced (CE) X-ray imaging techniques and to evaluate their correlation with histological biomarkers of angiogenesis in a subcutaneous C6 glioma model. Enhancement (E), iodine concentration (CI), and relative blood volume (rBV) were quantified from single- and dual-energy (SE and DE, respectively) micro-computed tomography (micro-CT) images, while rBV and volume transfer constant (Ktrans) were quantified from dynamic contrast-enhanced (DCE) planar images. CI and rBV allowed a better discernment of tumor regions from muscle than E in SE and DE images, while no significant differences were found for rBV and Ktrans in DCE images. An agreement was found in rBV for muscle quantified with the different imaging protocols, and in CI and E quantified with SE and DE protocols. Significant strong correlations (Pearson r > 0.7, p < 0.05) were found between a set of imaging parameters in SE images and histological biomarkers: E and CI in tumor periphery were associated with microvessel density (MVD) and necrosis, E and CI in the complete tumor with MVD, and rBV in the tumor periphery with MVD. In conclusion, quantitative imaging parameters obtained in SE micro-CT images could be used to characterize angiogenesis and necrosis in the subcutaneous C6 glioma model.

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New Technique for Detecting Cracked Teeth and Evaluating the Crack Depth by Contrast-enhanced Cone Beam Computed Tomography: an in Vitro Study

10.21203/rs.3.rs-1110699/v1 ◽

2021 ◽

Author(s):

Jie Zhou ◽

Jieni Fu ◽

Mo Xiao ◽

Yangyang Lv ◽

Fei Wu ◽

...

Keyword(s):

Computed Tomography ◽

Cone Beam Computed Tomography ◽

Crack Depth ◽

In Vitro Study ◽

New Technique ◽

Cone Beam ◽

Micro Ct ◽

Micro Computed Tomography ◽

Contrast Enhanced

Abstract Background: Develop a new technique based on contrast-enhanced cone beam computed tomography (CBCT) to improve the detection of cracked teeth and the accuracy of crack depth evaluation in vitro. Methods: We developed an in vitro artificial simulation model of cracked teeth. Pre-experimental CBCT (pre-CBCT), and micro-computed tomography (micro-CT) were first performed for all cracked teeth (n = 31). Contrast-enhanced CBCT was then performed by infiltrating the crack with ioversol under vacuum conditions. The results of pre-CBCT, micro-CT, and contrast-enhanced CBCT were recorded. SPSS v.26.0 software (IBM Corp, Somers, NY) and R software , version 3.6.0 (R Foundation for Statistical Computing; http://www.r-project.org/) and RStudio 1.1.463 (RStudio, PBC, Boston, MA, US) were used to perform the statistical analysis for the study.Results: The sensitivities of pre-CBCT and contrast-enhanced CBCT were 48.4%, and 67.7%, respectively. ICC value of crack depth as measured by pre-CBCT and contrast-enhanced CBCT was 0.847 (95% confidence interval:0.380-0.960; P < 0.001). The areas under ROC curves (AUC) of pre-CBCT and contrast-enhanced CBCT were different, the AUC of pre-CBCT was 0.958 (P = 0.000, 95% CI :0.843-1.074), and the AUC of enhanced CBCT was 0.979 (P = 0.000, 95% CI :0.921-1.037), and the difference was not statistically significant (Z=-0.707, P = 0.480). The ICC value of crack depth as measured by contrast-enhanced CBCT and micro-CT was 0.753 (95% CI: 0.248-0.911; P < 0.001). Conclusion: Contrast-enhanced CBCT under vacuum conditions with a contrast medium can only significantly improve the cracks detection rate of cracked teeth, but not measure the crack depths accurately.

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Dynamic Contrast-Enhanced Micro–Computed Tomography Correlates With 3-Dimensional Fluorescence Ultramicroscopy in Antiangiogenic Therapy of Breast Cancer Xenografts

Investigative Radiology ◽

10.1097/rli.0000000000000038 ◽

2014 ◽

Vol 49 (7) ◽

pp. 445-456 ◽

Cited By ~ 13

Author(s):

Thomas Pöschinger ◽

Anja Renner ◽

Fabian Eisa ◽

Michael Dobosz ◽

Steffen Strobel ◽

...

Keyword(s):

Breast Cancer ◽

Computed Tomography ◽

Antiangiogenic Therapy ◽

Micro Computed Tomography ◽

3 Dimensional ◽

Dynamic Contrast Enhanced ◽

Contrast Enhanced ◽

Breast Cancer Xenografts

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Porcine Ex Vivo Liver Phantom for Dynamic Contrast-Enhanced Computed Tomography

Investigative Radiology ◽

10.1097/rli.0b013e31821c0e84 ◽

2011 ◽

Vol 46 (9) ◽

pp. 586-593 ◽

Cited By ~ 9

Author(s):

Scott M. Thompson ◽

Juan C. Ramirez-Giraldo ◽

Bruce Knudsen ◽

Joseph P. Grande ◽

Jodie A. Christner ◽

...

Keyword(s):

Computed Tomography ◽

Ex Vivo ◽

Dynamic Contrast Enhanced ◽

Contrast Enhanced ◽

Enhanced Computed Tomography ◽

Liver Phantom ◽

Contrast Enhanced Computed Tomography

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Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

Applied Sciences ◽

10.3390/app11030891 ◽

2021 ◽

Vol 11 (3) ◽

pp. 891

Author(s):

Taylor Flaherty ◽

Maryam Tamaddon ◽

Chaozong Liu

Keyword(s):

Computed Tomography ◽

Bone Regeneration ◽

Subchondral Bone ◽

Volume Ratio ◽

Bone Volume ◽

Osteochondral Defects ◽

Micro Ct ◽

Micro Computed Tomography ◽

Trabecular Thickness ◽

Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

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Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

Scientific Reports ◽

10.1038/s41598-020-80404-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Javier Alba-Tercedor ◽

Wayne B. Hunter ◽

Ignacio Alba-Alejandre

Keyword(s):

Computed Tomography ◽

Significant Advance ◽

Micro Ct ◽

Diaphorina Citri ◽

Ct Reconstruction ◽

Internal Anatomy ◽

Micro Computed Tomography ◽

Citrus Leaf ◽

Teaching Aids ◽

Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

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MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

10.17504/protocols.io.bxw4ppgw ◽

2021 ◽

Author(s):

Eva Chatzinikolaou ◽

Kleoniki Keklikoglou

Keyword(s):

Computed Tomography ◽

High Resolution ◽

3D Imaging ◽

Imaging Technique ◽

Ct Scanning ◽

Micro Ct ◽

Micro Computed Tomography ◽

Gastropod Species ◽

Biological Studies ◽

Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

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