scholarly journals Preparation of 5-HMF in a DES/Ethyl N-Butyrate Two-Phase System

Catalysts ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 636
Author(s):  
Jinyan Lang ◽  
Junliang Lu ◽  
Ping Lan ◽  
Na Wang ◽  
Hongyan Yang ◽  
...  
Keyword(s):  
Cellulose Degradation ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Product Yield ◽  
Homogeneous System ◽  
Recycling Rate ◽  
Phase System ◽  
Separation System ◽  
Two Phase ◽  
Dissolution Reaction

In this paper, a two-phase system, formed by oxalic acid/choline chloride-based deep eutectic solvent (DES) and chosen extractants, was used as a dissolution–reaction–separation system, and metal chloride was used as a catalyst to study the degradation of cellulose to produce 5-hydroxymethylfurfural (5-HMF) and glucose. The effects of the amount of organic solvent and the reaction temperature on product yield, the repeated recycling of DES, the comparison between a two-phase system and a homogeneous system, and the mechanism of cellulose degradation to 5-HMF were investigated. The results show that ethyl n-butyrate has the best extraction effect on 5-HMF. Compared with the homogeneous system, the yield of 5-HMF and glucose in the two-phase system is significantly improved. At a temperature of 140 °C and a reaction time of 120 min, the yields of glucose and 5-HMF reached the maximum, which were 23.5% and 29.8%, respectively. After DES was reused three times, the yields of glucose and 5-HMF decreased greatly, indicating that the recycling rate of DES was low.

scholarly journals Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.

2017 ◽  
Author(s):  
Xifeng Zhang ◽  
Ji Zhang
Keyword(s):  
Ursolic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Phase System ◽  
Green Solvents ◽  
Active Components ◽  
Two Phase ◽  
Solid Ratio

Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.

scholarly journals Deep eutectic solvents aqueous two-phase system based ultrasonically assisted extraction of ursolic acid (UA) from Cynomorium songaricum Rupr.

2017 ◽  
Author(s):  
Xifeng Zhang ◽  
Ji Zhang
Keyword(s):  
Ursolic Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Deep Eutectic Solvents ◽  
Selective Extraction ◽  
Phase System ◽  
Green Solvents ◽  
Active Components ◽  
Two Phase ◽  
Solid Ratio

Deep eutectic solvents (DESs) are new green solvents that have attracted the attention of the scientific community mainly due to their unique properties and special characteristics, which are different from those of traditional solvents.A method based on ultrasonically assisted deep eutectic solvent aqueous two-phase systems( UAE-DES-ATPS) was developed for extracting ursolic acid (UA) from Cynomorium songaricum Rupr. Four different types of choline chloride-based DESs were prepared.Choline chloride-glucose (ChCl-Glu) exhibited good selective extraction ability. An optimum DES-ATPS of 36% (w/w) ChCl-Glu and 25% (w/w) K2HPO4 was considered to be a satisfactory system for extracting UA. Response surface methodology (RSM) method was used to optimize the extraction of UA using UAE-DES-ATPS. The optimum ultrasound-assisted conditions were as follows: solvent to solid ratio of 15:1 (g/g), ultrasound power of 470 W, and extraction time of 54 min. Compared with the conventional UAE method, the yields were basically the same, but the presented method had higher purity. The structure of UA did not change between pure UA and UA in the upper phase by UV–vis and FT-IR. This approach using ChCl-based DES-ATPS as a novel extraction system and ultrasound as a source of energy provided better choice for the separation of active components from other natural products.

Development of green betaine-based deep eutectic solvent aqueous two-phase system for the extraction of protein

Talanta ◽  
2016 ◽  
Vol 152 ◽  
pp. 23-32 ◽  
Author(s):  
Na Li ◽  
Yuzhi Wang ◽  
Kaijia Xu ◽  
Yanhua Huang ◽  
Qian Wen ◽  
...  
Keyword(s):  
Deep Eutectic Solvent ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Biosynthesis of Lactones From Diols Mediated by an Artificial Flavin

2021 ◽  
Author(s):  
Xiaowang Zhang ◽  
Zhuotao Tan ◽  
Chaojian Li ◽  
Siyu Qi ◽  
Mengjiao Xu ◽  
...  
Keyword(s):  
Product Yield ◽  
Product Inhibition ◽  
Phase System ◽  
Regeneration System ◽  
Two Phase ◽  
Promising Alternative ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Valuable Compounds ◽  
Chiral Lactones

Abstract Background: Lactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)+-dependent alcohol dehydrogenases-catalyzed oxidative lactonization of diols, which relies on the construction of an efficient NAD(P)+ regeneration system. Results: In this study, a novel system combining horse liver alcohol dehydrogenase (HLADH) with the synthetic bridged flavin cofactor was established for biosynthesis of lactones. The reaction conditions of this system were optimized and a variety of lactones including chiral lactones were efficiently obtained from various diols. Compared to the previously reported NAD(P)+-regeneration systems, this system showed better regeneration efficiency and product yield. A two-phase system was further applied to solve the problem of product inhibition, and 80% yield was obtained at the condition of 300 mM substrate.Conclusions: This study provides an efficient method to synthesis of lactones from diols under mild conditions. We believe this system will be a promising alternative to promote the synthesis of other valuable compounds.

scholarly journals Lipase-Catalyzed Production of Sorbitol Laurate in a “2-in-1” Deep Eutectic System: Factors Affecting the Synthesis and Scalability

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2759
Author(s):  
André Delavault ◽  
Oleksandra Opochenska ◽  
Laura Laneque ◽  
Hannah Soergel ◽  
Claudia Muhle-Goll ◽  
...  
Keyword(s):  
De Novo ◽  
Choline Chloride ◽  
Building Blocks ◽  
Flash Chromatography ◽  
Phase System ◽  
Eutectic System ◽  
Two Phase ◽  
Factors Affecting ◽  
Ester Formation ◽  
Wide Range

Surfactants, such as glycolipids, are specialty compounds that can be encountered daily in cleaning agents, pharmaceuticals or even in food. Due to their wide range of applications and, more notably, their presence in hygiene products, the demand is continuously increasing worldwide. The established chemical synthesis of glycolipids presents several disadvantages, such as lack of specificity and selectivity. Moreover, the solubility of polyols, such as sugars or sugar alcohols, in organic solvents is rather low. The enzymatic synthesis of these compounds is, however, possible in nearly water-free media using inexpensive and renewable building blocks. Using lipases, ester formation can be achieved under mild conditions. We propose, herein, a “2-in-1” system that overcomes solubility problems, as a Deep Eutectic System (DES) made of sorbitol and choline chloride replaces either a purely organic or aqueous medium. For the first time, 16 commercially available lipase formulations were compared, and the factors affecting the conversion were investigated to optimize this process, owing to a newly developed High-Performance Liquid Chromatography-Evaporative Light Scattering Detector (HPLC-ELSD) method for quantification. Thus, using 50 g/L of lipase formulation Novozym 435® at 50 °C, the optimized synthesis of sorbitol laurate (SL) allowed to achieve 28% molar conversion of 0.5 M of vinyl laurate to its sugar alcohol monoester when the DES contained 5 wt.% water. After 48h, the de novo synthesized glycolipid was separated from the media by liquid–liquid extraction, purified by flash-chromatography and characterized thoroughly by one- and two-dimensional Nuclear Magnetic Resonance (NMR) experiments combined to Mass Spectrometry (MS). In completion, we provide initial proof of scalability for this process. Using a 2.5 L stirred tank reactor (STR) allowed a batch production reaching 25 g/L in a highly viscous two-phase system.

scholarly journals Biosynthesis of lactones from diols mediated by an artificial flavin

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaowang Zhang ◽  
Zhuotao Tan ◽  
Chaojian Li ◽  
Siyu Qi ◽  
Mengjiao Xu ◽  
...  
Keyword(s):  
Product Yield ◽  
Product Inhibition ◽  
Phase System ◽  
Regeneration System ◽  
Two Phase ◽  
Promising Alternative ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Valuable Compounds ◽  
Chiral Lactones

Abstract Background Lactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)+-dependent alcohol dehydrogenases-catalyzed oxidative lactonization of diols, which relies on the construction of an efficient NAD(P)+ regeneration system. Results In this study, a novel system combining horse liver alcohol dehydrogenase (HLADH) with the synthetic bridged flavin cofactor was established for biosynthesis of lactones. The reaction conditions of this system were optimized and a variety of lactones including chiral lactones were efficiently obtained from various diols. Compared to the previously reported NAD(P)+-regeneration systems, this system showed better regeneration efficiency and product yield. A two-phase system was further applied to solve the problem of product inhibition, and 80% yield was obtained at the condition of 300 mM substrate. Conclusions This study provides an efficient method to synthesis of lactones from diols under mild conditions. We believe this system will be a promising alternative to promote the synthesis of other valuable compounds. Graphic abstract

scholarly journals Silver Nanoparticles and Polyphenol Inclusion Compounds Composites for Phytophthora cinnamomi Mycelial Growth Inhibition

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 76 ◽  
Author(s):  
Petruta Matei ◽  
Jesús Martín-Gil ◽  
Beatrice Michaela Iacomi ◽  
Eduardo Pérez-Lebeña ◽  
María Barrio-Arredondo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Growth Inhibition ◽  
Inclusion Compounds ◽  
Mycelial Growth ◽  
Water Solubility ◽  
Phytophthora Cinnamomi ◽  
Deep Eutectic Solvent ◽  
Phase System ◽  
Two Phase

Phytophthora cinnamomi, responsible for “root rot” or “dieback” plant disease, causes a significant amount of economic and environmental impact. In this work, the fungicide action of nanocomposites based on silver nanoparticles and polyphenol inclusion compounds, which feature enhanced bioavailability and water solubility, was assayed for the control of this soil-borne water mold. Inclusion compounds were prepared by an aqueous two-phase system separation method through extraction, either in an hydroalcoholic solution with chitosan oligomers (COS) or in a choline chloride:urea:glycerol deep eutectic solvent (DES). The new inclusion compounds were synthesized from stevioside and various polyphenols (gallic acid, silymarin, ferulic acid and curcumin), in a [6:1] ratio in the COS medium and in a [3:1] ratio in the DES medium, respectively. Their in vitro response against Phytophthora cinnamomi isolate MYC43 (at concentrations of 125, 250 and 500 µg·mL−1) was tested, which found a significant mycelial growth inhibition, particularly high for the composites prepared using DES. Therefore, these nanocomposites hold promise as an alternative to fosetyl-Al and metalaxyl conventional systemic fungicides.

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