scholarly journals Mycoplasma genitalium Infection and Chronic Inflammation in Human Prostate Cancer: Detection Using Prostatectomy and Needle Biopsy Specimens

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 212 ◽  
Author(s):  
Makito Miyake ◽  
Kenta Ohnishi ◽  
Shunta Hori ◽  
Akiyo Nakano ◽  
Ryuichi Nakano ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Sexually Transmitted Infection ◽  
Mycoplasma Genitalium ◽  
Detection Sensitivity ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Transmitted Infection ◽  
Prostate Hyperplasia ◽  
Sexually Transmitted ◽  
Biopsy Specimens

The evidence of association between sexually transmitted infection and prostatic inflammation in human prostate cancer (PCa) is limited. Here, we sought to examine the potential association of prostatic infection with the inflammatory environment and prostate carcinogenesis. We screened surgical and biopsy specimens from 45 patients with PCa against a panel of sexually transmitted infection-related organisms using polymerase chain reaction and examined the severity of intraprostatic inflammation by pathologic examination. Among tested organisms, the rate of Mycoplasma genitalium (Mg) infection was significantly different between the prostate cancer cohort and benign prostate hyperplasia (BPH) cohort (P = 0.03). Mg infection in the surgical specimens was associated with younger patients. The rate of extensive disease (pT2c–3b) was higher in Mg-positive patients than in Mg-negative patients (P = 0.027). No significant correlation was observed between Mg infection status and the grade of intraprostatic inflammation. The detection sensitivity of biopsy specimens was 61% for Mg and 60% for human papillomavirus (HPV)18, indicating possible clinical application of this material. A comprehensive understanding of the correlation between the urogenital microbiome and inflammation would facilitate the development of strategies for PCa prevention. Further studies are required to explore its clinical utility in recommendations of early re-biopsy, close follow-up, and treatment by antibiotics.

scholarly journals Partner notification and treatment outcomes among South African adolescents and young adults diagnosed with a sexually transmitted infection via laboratory-based screening

2020 ◽  
Vol 31 (7) ◽  
pp. 627-636 ◽  
Author(s):  
Pooja Chitneni ◽  
Mags Beksinska ◽  
Janan J Dietrich ◽  
Manjeetha Jaggernath ◽  
Kalysha Closson ◽  
...  
Keyword(s):  
Young Adults ◽  
Sexually Transmitted Infection ◽  
Trichomonas Vaginalis ◽  
Partner Notification ◽  
Mycoplasma Genitalium ◽  
Adolescents And Young Adults ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Care Cascade ◽  
Essential Components

Partner notification and treatment are essential components of sexually transmitted infection (STI) management, but little is known about such practices among adolescents and young adults. Using data from a prospective cohort study (AYAZAZI) of youth aged 16–24 years in Durban, South Africa, we assessed the STI care cascade across participant diagnosis, STI treatment, partner notification, and partner treatment; index recurrent STI and associated factors; and reasons for not notifying partner of STI. Participants completed laboratory-based STI screening ( Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis) at enrollment and at 12 months. Of the 37/216 participants with STI (17%), 27/37 (73%) were women and 10/37 (27%) were men. Median age was 19 years (IQR: 18–20). Of the participants with STI, 23/37 (62%) completed a Treatment and Partner Tracing Survey within 6 months of diagnosis. All survey participants reported completing STI treatment (100%), 17/23 (74%) notified a partner, and 6/23 (35%) reported partner treatment. Overall, 4/23 (11%) participants had 12-month recurrent C. trachomatis infection, with no association with partner notification or treatment. Stigma and lack of STI knowledge were reasons for not notifying partner of STI. STI partner notification and treatment is a challenge among youth. Novel strategies are needed to overcome barriers along the STI care cascade.

scholarly journals Expansion of Comprehensive Screening of Male Sexually Transmitted Infection Clinic Attendees with Mycoplasma genitalium and Trichomonas vaginalis Molecular Assessment: a Retrospective Analysis

2016 ◽  
Vol 55 (1) ◽  
pp. 321-325 ◽  
Author(s):  
Erik Munson ◽  
David Wenten ◽  
Sheila Jhansale ◽  
Mary Kay Schuknecht ◽  
Nicki Pantuso ◽  
...  
Keyword(s):  
Sexually Transmitted Infection ◽  
Trichomonas Vaginalis ◽  
Screening Program ◽  
Mycoplasma Genitalium ◽  
Transmitted Infection ◽  
Additional Specimen ◽  
Content Type ◽  
Sexually Transmitted ◽  
High Prevalence ◽  
Infection Clinic

ABSTRACTOf 1,493 encounters of males at a sexually transmitted infection (STI) clinic in a community with a high prevalence of STI,Chlamydia trachomatiswas detected in 8.7% andNeisseria gonorrhoeaewas detected in 6.6%. AdditionalTrichomonas vaginalisandMycoplasma genitaliumscreening found 17.4% and 23.9% of the encounters, respectively, to be positive for STI. STI agents were detected in 13.7% of urine specimens; addition of pharyngeal and rectal collections to the analysis resulted in detection of STI agents in 19.0% and 23.9% of encounters, respectively. A total of 101 (23.8%) encounters of identified STI involved sole detection ofM. genitalium. Expansion of the STI analyte panel (includingM. genitalium) and additional specimen source sampling within a comprehensive STI screening program increase identification of male STI carriers.

scholarly journals Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study

2020 ◽  
Author(s):  
Xiaohui Zhang ◽  
PZ Zhao ◽  
WJ Ke ◽  
LY Wang ◽  
Lai Sze Tso ◽  
...  
Keyword(s):  
Sexually Transmitted Infection ◽  
Clinical Symptoms ◽  
Mycoplasma Genitalium ◽  
Epidemiological Studies ◽  
Cross Sectional Study ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
Asymptomatic Patients ◽  
Tract Infections

Abstract Background: Mycoplasma genitalium (MG) causes urogenital tract infections and is associated with reproductive morbidity. Although MG has been reported across many regions and population groups, it is not yet routinely tested for in China. Our study contributes to current research by reporting the prevalence and correlates of MG infection in patients attending a sexually transmitted infection (STI) clinic in Guangdong from Jan 2017-May 2018.Methods: Urethral (from 489 men) and endo-cervical (from 189 women) samples, blood samples, and patient histories (via questionnaires) were collected. Doctors clinically diagnosed anogenital warts (GW) during the examination (n=678). The presence of MG was evaluated using an in-house via polymerase chain reaction protocol. We also tested all participants for herpes simplex virus-2 (HSV-2), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), syphilis and HIV. Univariate and multivariate logistic regression were used to evaluate factors associated with MG.Results: MG was detected in 7.2% (49/678) of the patients (men, 7.4%; women, 6.9%). The MG positivity rate was 14.2% among symptomatic patients, and 5.6% for asymptomatic patients , respectively. Only 36.7% (18/49) Mg positive patients were symptomatic. Among the MG-infected patients, 10.2% were co-infected with CT, 6.1% with NG, 8.2% with HSV-2, 4.1% with syphilis and 22.4% with GW. Presentation with clinical symptoms was significantly associated with MG infection [OR=2.52 (2.03-3.13)]. In our analysis, MG was not associated with other STIs.Conclusions: MG is a relatively common infection among individuals attending an STI clinic in Guangdong Province. Routine testing of symptomatic patients may be necessary, and more epidemiological studies are needed to provide evidence for future testing guidelines,

Lower mgpB diversity in macrolide-resistant Mycoplasma genitalium infecting men visiting two sexually transmitted infection clinics in Montpellier, France

2020 ◽  
Vol 76 (1) ◽  
pp. 43-47
Author(s):  
Jennifer Guiraud ◽  
Manon Lounnas ◽  
Anne Boissière ◽  
Chloé Le Roy ◽  
Eric Elguero ◽  
...  
Keyword(s):  
Sexual Behaviour ◽  
Sexually Transmitted Infection ◽  
Tandem Repeats ◽  
Sampling Site ◽  
Mycoplasma Genitalium ◽  
Macrolide Resistance ◽  
Fluoroquinolone Resistance ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Resistant Strains

Abstract Objectives Men engaged in high-risk sexual behaviour, such as MSM, are likely to be infected by resistant Mycoplasma genitalium strains. Understanding the transmission dynamics is challenging. We aimed to investigate the molecular epidemiology of M. genitalium in men visiting sexually transmitted infection (STI) clinics. Patients and methods Between June 2017 and February 2018, 95 M. genitalium-positive specimens from 78 men, including 76.9% MSM, visiting two STI clinics in Montpellier, France, were analysed for SNPs in the mgpB adhesin gene and number of tandem repeats in the MG_309 gene. Macrolide and fluoroquinolone resistance were determined. Typing results were compared with antibiotic resistance, sexual behaviour, sampling site, HIV pre-exposure prophylaxis (PrEP) usage and HIV status. Results Thirty-eight mgpB STs were identified, including 23 new STs, with ST4 being most prevalent. The mgpB/MG_309 typing method identified 52 genetic profiles, resulting in a discriminatory index of 0.979. Macrolide and fluoroquinolone resistance-associated mutations were detected in 58.3% and 10.8% of patients, respectively. The macrolide resistance rate was higher among MSM than among men who have sex with women only (68.4% versus 9.1%; adjusted OR, 1.57; 95% CI, 1.13–2.18; P = 0.007). A lower mgpB diversity of 0.870 was found among macrolide-resistant strains in comparison with 0.978 in macrolide-susceptible strains, with an over-representation of mgpB ST62 and ST153. Conclusions Although macrolide resistance spread appears polyclonal in M. genitalium, the lower diversity of mgpB types among macrolide-resistant strains may reflect the easier spread of a few specific mgpB types or the occurrence of sexual networks among MSM.

High prevalence and resistance rates of Mycoplasma genitalium among patients visiting two sexually transmitted infection clinics in the Netherlands

2021 ◽  
pp. 095646242199928
Author(s):  
David J Hetem ◽  
Sophie Kuizenga Wessel ◽  
Sylvia M Bruisten ◽  
Joyce F Braam ◽  
Martijn S van Rooijen ◽  
...  
Keyword(s):  
Sexually Transmitted Infection ◽  
Mycoplasma Genitalium ◽  
Macrolide Resistance ◽  
Obstetric Outcomes ◽  
Prevalence Rates ◽  
Transmitted Infection ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
High Prevalence ◽  
Resistance Rates

Mycoplasma genitalium is a well-known cause of urethritis in men and has been associated with cervicitis, pelvic inflammatory disease, and adverse obstetric outcomes in women. In this cross-sectional study, we determined the current prevalence of M. genitalium infection and the rate of macrolide resistance in M. genitalium isolates, in patients visiting two large Dutch sexually transmitted infection (STI) clinics, to evaluate whether the recommendations in Dutch guidelines should be revised. In addition, risk factors for M. genitalium were identified. In total, 3225 patients were included. M. genitalium prevalence rates were 13.8% for all patients; 20.1% for men who have sex with men, 8.2% for men who have sex with women, and 12.6% for women. Macrolide resistance-associated mutations were detected in 66% of the patients infected with M. genitalium. Age, educational level, country of origin, number of sexual partners, HIV-positivity, infection with Neisseria gonorrhoeae, and urethral symptoms in men were independently associated with M. genitalium infection. In conclusion, we found very high prevalence rates and macrolide resistance rates of M. genitalium in patients visiting STI clinics.

scholarly journals Mycoplasma genitalium Infection in Women Attending a Sexually Transmitted Infection Clinic

2012 ◽  
Vol 39 (9) ◽  
pp. 706-709 ◽  
Author(s):  
Victoria L. Mobley ◽  
Marcia M. Hobbs ◽  
Karen Lau ◽  
Barbara S. Weinbaum ◽  
Damon K. Getman ◽  
...  
Keyword(s):  
Sexually Transmitted Infection ◽  
Mycoplasma Genitalium ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Sexually Transmitted Infection Clinic ◽  
Infection Clinic

scholarly journals Human Papillomavirus (HPV) Infection As an Emerging Risk Factor in Prostate Cancer

2018 ◽  
Vol 4 (Supplement 2) ◽  
pp. 24s-24s
Author(s):  
N. Singh ◽  
A. Josefsson ◽  
S. Hussain ◽  
J. Hugosson
Keyword(s):  
Prostate Cancer ◽  
Sexually Transmitted Infection ◽  
Hpv Infection ◽  
Clinical Staging ◽  
Clinicopathological Parameters ◽  
Prognostic Impact ◽  
Preoperative Radiation ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Prostate Carcinogenesis

Background: Epidemiologic investigations confirm that prostate tissue is prone to sexually transmitted infection and human papilloma virus (HPV) is the most common sexually transmitted infection. Owing to the controversy on the role of HPV infection in prostate carcinogenesis, it is appropriate to determine and validate the prevalence of HPV infection in a controlled prospective study, and its role in prostate carcinogenesis. It is crucial to investigate the prognostic impact of HPV infection in prostate cancer from a clinical point of view. Aim: The overall aim of our research is to address the possibility of using therapeutic interventions against HPV infection in young boys to prevent the development of prostate cancer in their older age. Establishment of clinical importance of HPV infection in prostate cancer and its prognostic impact for overall survival. Methods: The prostate cancer tissue specimens were obtained from Sahlgrenska University Hospital, Sahlgrenska Academy (SA), Gothenburg, Sweden. The exclusion criteria included patients undergoing any preoperative radiation or chemotherapy. Only histopathologically confirmed cases were processed for DNA, protein and RNA extraction. Histopathological grades and clinical staging was evaluated by pathologists using the Gleason scoring system for prostate cancer. The study is approved by the ethics committee of the institute. High molecular-weight genomic DNA was isolated from tumor/control tissue samples and were subjected to PCR genotyping for detection of the viral DNA. Cases and controls was compared using univariate methods. An independent t-test was performed for the comparison of clinicopathological parameters. Results: The pilot study identified HPV infection in advanced grade of prostate cancer cases in Sweden. HPV infection was identified in 57% of the prostate cancer cases with advanced pathologic grade in Swedish men compared with 11% in the normal controls. The investigation comprised of detailed analysis of the correlation between the clinical parameters and HPV genotyping. Conclusion: The research investigation substantiates the clinical significance of HPV infection in prostate carcinogenesis that has been underestimated till date. The research investigation was funded by Swedish Research Council (Vetenskaprådet) Grant no. 2015-06705.

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