scholarly journals Equine Penile Squamous Cell Carcinomas as a Model for Human Disease: A Preliminary Investigation on Tumor Immune Microenvironment

Cells ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 2364 ◽  
Author(s):  
Ilaria Porcellato ◽  
Samanta Mecocci ◽  
Luca Mechelli ◽  
Katia Cappelli ◽  
Chiara Brachelente ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Plasma Cells ◽  
Preliminary Investigation ◽  
Squamous Cell Carcinomas ◽  
Dna And Rna ◽  
Tumor Immune Microenvironment ◽  
Peritumoral Stroma ◽  
Histology And Immunohistochemistry

Penile squamous cell carcinomas (SCCs) are common tumors in older horses, with poor prognosis mostly due to local invasion and recurrence. These tumors are thought to be mainly caused by Equus caballus papillomavirus type 2 (EcPV-2). The aim of this study is to characterize the tumor immune environment (TIME) in equine penile tumors. Equine penile epithelial tumors (17 epSCCs; 2 carcinomas in situ, CIS; 1 papilloma, P) were retrospectively selected; immune infiltrate was assessed by histology and immunohistochemistry; RT-qPCR tested the expression of selected chemokines and EcPV-2 DNA and RNA. The results confirmed EcPV-2-L1 DNA in 18/20 (90%) samples. L1 expression was instead retrieved in 13/20 cases (65%). The samples showed an increased infiltration of CD3+lymphocytes, macrophages (MAC387; IBA1), plasma cells (MUM1), and FoxP3+lymphocytes in the intra/peritumoral stroma when compared to extratumoral tissues (p < 0.05). Only MAC387+neutrophils were increased in EcPV-2high viral load samples (p < 0.05). IL12/p35 was differentially expressed in EcPVhigh and EcPVlow groups (p = 0.007). A significant decrease of IFNG and IL2 expression was highlighted in TGFB1-positive samples (p < 0.05). IBA1 and CD20 were intratumorally increased in cases where IL-10 was expressed (p < 0.005). EpSCCs may represent a good spontaneous model for the human counterpart. Further prospective studies are needed in order to confirm these preliminary results.

Equine Penile Squamous Cell Carcinomas Are Associated With the Presence of Equine Papillomavirus Type 2 DNA Sequences

2011 ◽  
Vol 48 (6) ◽  
pp. 1190-1194 ◽  
Author(s):  
C. G. Knight ◽  
J. S. Munday ◽  
J. Peters ◽  
M. Dunowska
Keyword(s):  
Polymerase Chain Reaction ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Dna Sequences ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Polymerase Chain

Forty cases of equine penile disease were screened with polymerase chain reaction for the presence of papillomaviral DNA. Cases consisted of 20 squamous cell carcinomas (average age of horse, 23.9 years) and 20 non–squamous cell carcinoma diseases (average age of horse, 13.3 years). All horses but one originated from the Northeastern United States. Breeds were not recorded. As based on MY09/MY11 consensus primers, DNA sequences from equine papillomavirus type 2 were amplified from 9 of 20 horses (45%) with penile squamous cell carcinoma and only 1 of 20 horses (5%) with non–squamous cell carcinoma penile disease. Equine papillomavirus type 2 DNA was the only papillomaviral DNA amplified from any of the 40 horses. Tissues from the 10 horses in which papillomaviral DNA was detected by polymerase chain reaction were also screened with in situ hybridization and immunohistochemistry. The presence of papillomavirus was demonstrated in a subset of these by in situ hybridization (6 of 10) and immunohistochemistry (1 of 10). This report describes a possible association between equine penile squamous cell carcinomas and equine papillomavirus type 2. This study is also the first report of equine papillomavirus type 2 infection in North American horses.

Use of fluorescence in situ hybridization to detect Felis catus papillomavirus type 2 in feline Bowenoid in situ carcinomas

2018 ◽  
Vol 21 (6) ◽  
pp. 575-580 ◽  
Author(s):  
Lauren E Demos ◽  
John S Munday ◽  
Christian E Lange ◽  
Mark D Bennett
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Felis Catus ◽  
Pcr Detection ◽  
Lesion Formation ◽  
Site Specific

Objectives Papillomaviruses (PVs) are ubiquitous host- and site-specific viruses. PV infections in cats are associated with oral papillomas, viral plaques, Bowenoid in situ carcinomas (BISCs), squamous cell carcinomas and sarcoids; this association is primarily based on PCR detection of PV DNA within said lesions. PV DNA is frequently detectable on normal feline skin; thus, it is possible that some of the implicated DNA is commensal rather than associated with lesion formation. Therefore, the aim of the present study was to use fluorescence in situ hybridization (FISH) to localize PV DNA within feline BISCs, to provide additional evidence that PV infection may influence the development of these neoplasms. Methods FISH probes targeting Felis catus papillomavirus type 2 (FcaPV2) DNA were used to localize FcaPV2 DNA within 42 BISCs from which FcaPV2 DNA had previously been amplified via PCR. Results Fifteen of 42 BISC lesions (35.7%) demonstrated intralesional FcaPV2 using FISH. Probe annealing was predominantly located within the nuclei of koilocytes found in the upper strata of the epidermis. Probes were typically scattered multifocally within the lesions; most commonly this was near the periphery of the BISCs. Conclusions and relevance These results confirm that a proportion of BISCs contain FcaPV2 DNA. These results further support a causative association between FcaPV2 and BISCs in cats.

scholarly journals Enhanced Expression of Podoplanin in Oral Carcinomas in situ and Squamous Cell Carcinomas

Pathobiology ◽  
2011 ◽  
Vol 78 (3) ◽  
pp. 171-180 ◽  
Author(s):  
Akinori Funayama ◽  
Jun Cheng ◽  
Satoshi Maruyama ◽  
Manabu Yamazaki ◽  
Takanori Kobayashi ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Enhanced Expression

Transcriptome and cytogenetic profiling analysis of matched in situ/invasive cutaneous squamous cell carcinomas from immunocompetent patients

2019 ◽  
Vol 58 (3) ◽  
pp. 164-174 ◽  
Author(s):  
Irene García-Díez ◽  
Inmaculada Hernández-Muñoz ◽  
Eugenia Hernández-Ruiz ◽  
Lara Nonell ◽  
Eulàlia Puigdecanet ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Profiling Analysis ◽  
Immunocompetent Patients

scholarly journals Comparison of prevalence of Felis catus papillomavirus type 2 in squamous cell carcinomas in cats between Taiwan and Japan

2021 ◽  
Author(s):  
Nanako YAMASHITA-KAWANISHI ◽  
Chia Yu CHANG ◽  
James K CHAMBERS ◽  
Kazuyuki UCHIDA ◽  
Katsuaki SUGIURA ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Felis Catus

Vulvar and Vaginal Cancers

2017 ◽  
Author(s):  
Margaret M. Madeleine ◽  
Lisa G. Johnson
Keyword(s):  
Risk Factors ◽  
Human Papillomavirus ◽  
Cigarette Smoking ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Invasive Cancer ◽  
High Grade ◽  
Precursor Lesions ◽  
Shared Risk

Vulvar and vaginal cancers are rare and predominantly involve squamous cell carcinomas. Some studies combine these cancers, presumably because of their rarity, anatomic proximity, and shared risk factors. Major risk factors include human papillomavirus (HPV) and cigarette smoking. This chapter explores the similarities and important differences in etiology between these cancer sites. In addition to its focus on invasive cancer, the chapter also discusses high-grade precursor lesions, or in situ disease, that sometimes progress to cancer and must, therefore, be treated.

scholarly journals The Situation of Cervical Cancers in the Context of Female Genital Cancer Clustering and Burden of Disease in Arad County, Romania

2019 ◽  
Vol 8 (1) ◽  
pp. 96 ◽  
Author(s):  
Ana-Liana Tataru ◽  
Gheorghe Furau ◽  
Jompan Afilon ◽  
Cringu Ionescu ◽  
Mihai Dimitriu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell ◽  
Histopathological Examination ◽  
Squamous Cell Carcinomas ◽  
Point Of View ◽  
Clinical Staging ◽  
Cervical Cancers ◽  
Genital Cancer ◽  
Disability Adjusted Life

Romania has the highest incidence of cervical cancer morbidity and mortality in Europe. This study identifies the major clusters for genital cancers, observes the features of genital and cervical cancer, and determines the extent to which cancer is a contributor to total Disability-Adjusted Life Year (DALY). Spatial analysis used Besag and Newell’s method for genital cancer distribution, prevalence considered Arad County patients records (2008–2017), and DALY was determined according to WHO methodology and GLOBOCAN 2013 data. Diagnosis was established by histopathological examination of diagnostic biopsies or tissues obtained by surgical procedures, followed by clinical staging. 1695 women were recorded with genital cancer. Of these, 14.9% of lesions were in situ (n = 252) and 74.20% of cases were recorded in stage III or IV (n = 1258) (p < 0.0001). Over 90% of cervical cancers were squamous cell carcinomas (n = 728), 33.76% of endometrial cancers were adenocarcinomas in situ (n = 131), 32.42% of ovarian cancers were serous adenocarcinomas (n = 131), and 70.58% of vulvar cancers were squamous cell carcinomas (n = 48) (p < 0.0001). DALY/1000 was 67.2 for genital cancers and 33 for cervical cancers. From the point of view of Romanian women, cervical cancer remains one of the major problems that need to be dealt with and access to optimal treatment proves to be extremely limited.

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