scholarly journals The Influence of Serum Sample Storage Conditions on Selected Laboratory Parameters Related to Oxidative Stress: A Preliminary Study

Diagnostics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
Lilla Pawlik-Sobecka ◽  
Katarzyna Sołkiewicz ◽  
Izabela Kokot ◽  
Aleksandra Kiraga ◽  
Sylwia Płaczkowska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Sample ◽  
Storage Conditions ◽  
Wilcoxon Test ◽  
Reference Ranges ◽  
Sample Storage ◽  
Serum Samples ◽  
The Stability ◽  
The Individual ◽  
The Impact

The present work aims at accessing the stability of biological material stored for diagnostic and scientific purposes. The influence of the temperature, storage time, and cyclic thawing on concentration stability of selected oxidative stress parameters in human serum was investigated. The study group consisted of 20 serum samples collected from healthy volunteers aged 18–52. The parameters whose reference ranges were not determined and to which validated determination methods did not correspond were examined by manual methods (FRAP and AOPP). Automatic methods were used to determine routine laboratory tests (albumin, total protein, bilirubin, uric acid) using the Konelab 20i® analyzer. The samples were stored at various temperatures (room temperature, 4 °C, −20 °C, −80 °C) for max 6 months and were subjected to cyclic thawing at 1 month intervals. In order to check whether any differences between the concentrations of the studied parameters existed when the samples were stored in various conditions, the paired Student t-test or Wilcoxon test and comparison to desirable bias were applied. Based on the obtained results, it was found that the temperature and time of serum sample storage significantly affected the stability of the analyzed parameters and determined different shelf lives of serum samples for oxidative stress examination. Therefore, continuing the investigation concerning the impact of storage conditions on various serum parameters seems justified due to the discrepancy between the individual results obtained by different researchers and the inconsistencies between the results of scientific research and the applicable recommendations.

The Impact of Storage Conditions on the Stability of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12 in Human Milk

2017 ◽  
Vol 12 (9) ◽  
pp. 566-569 ◽  
Author(s):  
Anastasia Mantziari ◽  
Juhani Aakko ◽  
Himanshu Kumar ◽  
Satu Tölkkö ◽  
Elloise du Toit ◽  
...  
Keyword(s):  
Human Milk ◽  
Lactobacillus Rhamnosus ◽  
Storage Conditions ◽  
Lactobacillus Rhamnosus Gg ◽  
Bifidobacterium Animalis ◽  
The Stability ◽  
The Impact

scholarly journals SAT-166 Advantage and Trustworthy of Cortisol and Dexamethasone Evaluation in Different Biological Matrices in Patients with Adrenal Masses

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Luana Lionetto ◽  
Roberta Maggio ◽  
Pina Lardo ◽  
Donatella De Bernardini ◽  
Fabiola Cipolla ◽  
...  
Keyword(s):  
Salivary Cortisol ◽  
Adrenal Incidentaloma ◽  
Serum Cortisol ◽  
Reference Ranges ◽  
Serum Samples ◽  
Late Night ◽  
Liquid Chromatography Tandem Mass ◽  
Adrenal Masses ◽  
Suppression Test ◽  
The Individual

Abstract Biochemical function of adrenal masses is currently based on 1mg post-overnight dexamethasone suppression test (pDST). Several approaches are recently developed, in order to reduce false positive/negative samples, only in retrospective series. They are based on the correlation of some different parameters, i.e. late-night salivary cortisol (LNSC) vs serum and salivary cortisol pDST; LNSC vs serum and salivary cortisol and serum dexamethasone pDST; LNSC and cortisone vs serum cortisol and salivary cortisol and cortisone pDST. Although these findings offer a better diagnostic performance, several conditions are still disappointed. No information is traceable about the harvest time of diurnal salivary and serum samples and no study include neither the levels of salivary nor urinary dexamethasone pDST. Aim of our study is to combine all these strategies in order to avoid the underestimated biases and obtain more precise information about the true “cortisol condition” of the patients. To reach this purpose we assess both cortisol and dexamethasone concentrations in several samples: saliva at 11PM before the drug administration, diurnal saliva and serum at 8AM and also the urine collection from 11PM to 8AM. Analytes levels are measured using a validated liquid chromatography-tandem mass spectrometry method. In this study we included 20 subjects without morphological adrenal alteration (MRI assessment), dyslipidemia, hypertension and impaired glucose tolerance (healthy controls) and 20 patients with adrenal incidentaloma showing different cortisol levels ranging from normal to ACTH-independent hypercortisolism. In both series, LNSC were similar to salivary cortisol pDST, even if they were greater in the patients with adrenal incidentalomas and subclinical cortisol secretion. Serum dexamethasone levels were in reference ranges, while salivary and urinary dexamethasone found in these matrices require additional sample numbers in order to establish appropriate cut-offs. Our preliminary results suggest that the combination of these findings could represent an improvement to assess the individual cortisol status.

Systemic perturbation of cytokine and chemokine networks in Erdheim-Chester disease: a single-center series of 37 patients

Blood ◽  
2011 ◽  
Vol 117 (10) ◽  
pp. 2783-2790 ◽  
Author(s):  
Laurent Arnaud ◽  
Guy Gorochov ◽  
Frédéric Charlotte ◽  
Virginie Lvovschi ◽  
Christophe Parizot ◽  
...  
Keyword(s):  
Interferon Γ ◽  
Interferon Α ◽  
Single Center ◽  
Serum Samples ◽  
Individual Level ◽  
Erdheim Chester Disease ◽  
The Individual ◽  
The Impact ◽  
Erdheim Chester ◽  
Chester Disease

Abstract Immunopathogenesis of Erdheim-Chester disease (ECD), a rare non–Langerhans cell histiocytosis, is poorly known. In previous studies, various cytokines were detected in ECD lesions, presumably orchestrating lesional histiocyte recruitment. Because ECD lesions are frequently associated with systemic symptoms, we postulated that underlying global immune perturbations might also be revealed. We quantitatively analyzed 23 cytokines in serum samples obtained from a large single-center cohort of 37 patients with ECD, and studied the impact of treatment on cytokine production. IL-6, IL-12, interferon-α (IFN-α), and monocyte chemotactic protein-1 (MCP-1) levels were significantly higher in untreated patients than in controls, whereas interferon-γ (IFN-γ) inducible protein 10, IL-12, MCP-1, and IL-1 receptor antagonist were found significantly increased in IFN-α–treated patients. A biomathematical approach was used to rationalize multiparameter data, to generate new hypotheses, and identify global control pathways. Interestingly, cytokine profiles proved to be particularly stable at the individual level, and an “ECD signature” further distinguished patients from controls, based on their production of IFN-α, IL-12, MCP-1, IL-4, and IL-7. Altogether, our data underline the systemic immune Th-1–oriented perturbation associated with this condition and provide clues for the choice of more focused therapeutic agents in this rare disease with noncodified therapeutic management.

scholarly journals Short-Term Stability of Biomarkers of Oxidative Stress and Antioxidant Status in Human Serum

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Eugène H. J. M. Jansen ◽  
Piet K. Beekhof ◽  
Johannes W. J. M. Cremers ◽  
Dale Viezeliene ◽  
Vladimira Muzakova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum ◽  
Antioxidant Status ◽  
Small Deviation ◽  
Storage Conditions ◽  
Short Term ◽  
Serum Samples ◽  
Antioxidant Assays ◽  
And Performance ◽  
Short Time

The oxidation and antioxidant status of serum are often determined in serum samples which have been frozen for some time. The oxidative stress process is prone to fast alterations in the sample because of the possible instability of the reactants. Here one oxidation assay (ROM) and three antioxidant assays (FRAP, TAS, and BAP) have been tested on their performance and stability at short-time storage. The most commonly used temperatures for storage and handling of serum samples (+4 and +20°C) were selected. In three short-term studies in which the storage time varied between 3 and 48 hrs the performance of these assays were tested on human serum samples. The general conclusion is that most assays performed well and gave stable results during 2 days of storage of the samples at both temperatures. Only the FRAP and TAS assays showed a small deviation at some storage conditions. In conclusion, handling of serum samples at +4 and +20°C during short-time periods did not affect the quality and performance of the oxidation and antioxidant assays during day-to-day analyses.

scholarly journals Stability of amino acids and related amines in human serum under different preprocessing and pre-storage conditions based on iTRAQ®-LC-MS/MS

2020 ◽  
Author(s):  
Zhuoling An ◽  
Chen Shi ◽  
Pengfei Li ◽  
Lihong Liu
Keyword(s):  
Amino Acids ◽  
Human Serum ◽  
Storage Conditions ◽  
Serum Samples ◽  
Freeze Thaw ◽  
Treatment Measures ◽  
Isobaric Tagging ◽  
Pre Treatment ◽  
The Stability ◽  
Immediate Analysis

AbstractAmino acids analysis or metabonomics requires abundant serum/plasma samples collection and samples storage has become inevitable given the limited capacity for immediate analysis. Currently, most of the existing studies on metabolites stability during sample storage focused on long-term and short-term stability, while many functional amino acids might be ignored due to the poor sensitivity and detection of analysis methods. Here, we attempted to elucidate the stability of amino acids and related amines as comprehensive as possible in human serum following different preprocessing and pre-storage procedures. Pooled, fasting serum samples were collected and stored at 4 °C and 22 °C respectively after a delay in sample processing (0, 1, 2, 4, 8, 12 and 24 hours) and underwent freeze-thaw cycles for three times at −80 °C. The concentration of amino acids and related amines were quantified using isobaric tagging reagent iTRAQ®-LC-MS/MS. Approximately 54.84 %, 58.06 % and 48.39 % of detectable and target analytes altered at 4 °C and 22 °C during pre-treatment and freeze-thaw cycles. Some amino acids which are not stable and relatively stable were found. Our study provided detailed profiles and suggestions for amino acids in human serum corresponding to diverse collection and pre-treatment measures.

scholarly journals Influence of Sample Type and Storage Conditions on Soluble CD40 Ligand Assessment

2006 ◽  
Vol 52 (5) ◽  
pp. 888-891 ◽  
Author(s):  
Michael Weber ◽  
Birgitt Rabenau ◽  
Michael Stanisch ◽  
Albrecht Elsaesser ◽  
Vesselin Mitrovic ◽  
...  
Keyword(s):  
Cd40 Ligand ◽  
Storage Conditions ◽  
Sample Type ◽  
Serum Samples ◽  
Soluble Cd40 Ligand ◽  
Coronary Syndromes ◽  
Edta Plasma ◽  
Few Data ◽  
The Impact ◽  
And Storage

Abstract Background: Several studies have consistently shown that soluble CD40 ligand (sCD40L) concentrations are increased in patients with acute coronary syndromes and can serve as a biomarker for risk stratification. However, few data are available on preanalytic conditions that impact sCD40L values. Thus, the aim of our prospective study was to evaluate the impact of sampling techniques and storage conditions on sCD40L concentrations. Methods: We included a total of 30 patients with no, stable, or unstable coronary heart disease. Blood samples were collected in gel-filled tubes without additives, in EDTA-filled tubes, and in citrate-filled tubes and were kept at various storage conditions. Results: Median (interquartile range) sCD40L values at baseline were higher in serum samples [5.29 (3.89–6.33) μg/L] than in either EDTA plasma [0.78 (0.39–1.12) μg/L; P <0.001] or citrate plasma [0.37 (0.22–0.51) μg/L; P <0.001]. Serum values increased with delayed processing [7.94 (5.97–9.62) μg/L after 1.5 h (P <0.001) vs baseline; 10.55 (7.58–11.55) μg/L after 3 h (P <0.001) vs baseline]. However, after centrifugation, sCD40L values remained stable for all 3 sample types. Conclusion: Plasma, but not serum, samples are appropriate for sCD40L measurements. In general, preanalytic conditions are critical in the assessment of sCD40L concentrations and thus should be carefully considered for future studies.

scholarly journals Effect of Repeated Freeze–Thaw Cycles on Influenza Virus Antibodies

Vaccines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 267
Author(s):  
Alessandro Torelli ◽  
Elena Gianchecchi ◽  
Martina Monti ◽  
Pietro Piu ◽  
Irene Barneschi ◽  
...  
Keyword(s):  
World Health ◽  
Who Guidelines ◽  
Serum Samples ◽  
Serological Tests ◽  
Freeze Thaw ◽  
Before And After ◽  
The Stability ◽  
Health Organization ◽  
Internal Procedures ◽  
The Impact

Background: Vaccine effectiveness relies on various serological tests, whose aim is the measurement of antibody titer in serum samples collected during clinical trials before and after vaccination. Among the serological assays required by the regulatory authorities to grant influenza vaccine release there are: Hemagglutination inhibition (HAI), microneutralization (MN), and Single Radial Hemolysis (SRH). Although antibodies are regarded to be relatively stable, limited evidences on the effect of multiple freeze–thaw cycles on the stability of antibodies in frozen serum samples are available so far. In view of this, the present paper aimed to evaluate the impact of multiple freeze–thaw cycles on influenza antibody stability, performing HAI, MN and SRH assays. Methods: Ten serum samples were divided into 14 aliquots each, stored at −20 °C and taken through a total of 14 freeze–thaw cycles to assess influenza antibody stability. Each assay measurement was carried out following internal procedures based on World Health Organization (WHO) guidelines. Results: No statistically significant effect of 14 freeze–thaw cycles on antibody stability, measured through three different assays, was observed. Conclusions: Collectively, these data demonstrated that specific influenza antibody present in serum samples are stable up to 14 freeze–thaw cycles.

scholarly journals PAR Index in the Evaluation of the Stability of the Orthodontic Treatment Results. A Review

2006 ◽  
Vol 49 (4) ◽  
pp. 203-207 ◽  
Author(s):  
Chaitra Ramanathan
Keyword(s):  
Orthodontic Treatment ◽  
Treatment Results ◽  
Quality Of Work ◽  
Par Index ◽  
The Stability ◽  
The Individual ◽  
Individual Scores ◽  
The Impact

The evaluation of the treatment results is normally done to estimate the nature and quality of work, so that justice can be done to the work that we do and also that the patients will be satisfied. The primary motive of every orthodontist should be to treat the patient effectively and successfully with long lasting results. Thus the patients are to be assessed, using an appropriate method. PAR index was developed in the recent years to evaluate the treatment results and it is considered as a simple, objective and a reliable manner for evaluating the stability after orthodontic treatment. The index can be applied to different components of the dentition and scores are applied to each component after which the individual scores are multiplied with their respective weightings to balance the impact of the individual components of the overall result. They are then summed up to establish an overall total. In this manner, the method was carried out for the study casts of the three different phases of the treatment i.e. before the onset of the treatment, immediately after treatment and 2 years after treatment for assessing the stability after orthodontic treatment.

Stability of Chlorophenols and Their Acetylated Derivatives in Water: Sample Storage Procedures

2014 ◽  
Vol 97 (1) ◽  
pp. 179-182
Author(s):  
Paulo de Morais ◽  
Teodor Stoichev ◽  
M Clara P Basto ◽  
Pedro N Carvalho ◽  
M Teresa S D Vasconcelos
Keyword(s):  
Sodium Chloride ◽  
Acetic Anhydride ◽  
Water Samples ◽  
Storage Conditions ◽  
Storage Space ◽  
Sample Storage ◽  
Different Temperatures ◽  
Low Levels ◽  
The Stability

Abstract The determination of chlorophenols (CPs) in water samples is a subject of increasing interest. Reduction of sample storage space and the stability of CPs when present at very low levels are still problems that deserve research. The stability of CPs at ng/L levels at different temperatures and in the presence or absence of sodium carbonateand acetic anhydride was studied for up to 39 days. Stable and reproducible CP concentrations for about a month of storage in both river and wastewater were achieved in two storage conditions as follows: at –18°C with addition of 10% sodium chloride; and at 4°C with addition of both 10% sodium chloride and 10 mg/mL sodium carbonate. These sample treatments are good alternatives to the immobilization of CPs on SPE cartridges in terms of both analyte stability and saving of storage space.

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