scholarly journals Novel Histomorphometrical Approach to Evaluate the Integration Pattern and Functionality of Barrier Membranes

2021 ◽  
Vol 9 (11) ◽  
pp. 127
Author(s):  
Nicola Ottenbacher ◽  
Said Alkildani ◽  
Tadas Korzinskas ◽  
Jens Pissarek ◽  
Christian Ulm ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Study Groups ◽  
Tissue Response ◽  
Cellular Migration ◽  
Surrounding Tissue ◽  
Standing Time ◽  
Barrier Membranes ◽  
Cellular Invasion ◽  
Barrier Membrane ◽  
Post Implantation

GBR (guided bone regeneration) is a standard procedure for building up bony defects in the jaw. In this procedure, resorbable membranes made of bovine and porcine collagen are increasingly being used, which, in addition to many possible advantages, could have the potential disadvantage of a shorter barrier functionality, especially when augmenting large-volume defects. Thus, it is of importance to evaluate the integration behavior and especially the standing time of barrier membranes using specialized methods to predict its respective biocompatibility. This study is intended to establish a new histomorphometrical analysis method to quantify the integration rate of collagen-based barrier membranes. Three commercially available barrier membranes, i.e., non-crosslinked membranes (BioGide® and Jason® membrane), a ribose-crosslinked membrane (Ossix® Plus), and a newly developed collagen–hyaluronic acid-based (Coll-HA) barrier membrane were implanted in the subcutaneous tissue of 48 6–8-week-old Wistar rats. The explants, after three timepoints (10, 30, and 60 days), were processed and prepared into histological sections for histopathological (host tissue response) and histomorphometrical (cellular invasion) analyses. 10 days after implantation, fragmentation was not evident in any of the study groups. The sections of the Coll-HA, Jason® and BioGide® membranes showed a similar mild inflammatory reaction within the surrounding tissue and an initial superficial cell immigration. Only in the Ossix® Plus group very little inflammation and no cell invasion was detected. While the results of the three commercially available membranes remained intact in the further course of the study, only fragments of the Coll-HA membrane were found 30 and 60 days after implantation. Histomorphometrically, it can be described that although initially (at 10 days post-implantation) similar results were found in all study groups, after 30 days post-implantation the cellular penetration depth of the hyaluronic acid-collagen membrane was significantly increased with time (**** p < 0.0001). Similarly, the percentage of cellular invasion per membrane thickness was also significantly higher in the Coll-HA group at all timepoints, compared to the other membranes (**** p < 0.0001). Altogether, these results show that the histomorphometrical analysis of the cellular migration can act as an indicator of integration and duration of barrier functionality. Via this approach, it was possible to semi-quantify the different levels of cellular penetration of GBR membranes that were only qualitatively analyzed through histopathological approaches before. Additionally, the results of the histopathological and histomorphometrical analyses revealed that hyaluronic acid addition to collagen does not lead to a prolonged standing time, but an increased integration of a collagen-based biomaterial. Therefore, it can only partially be used in the dental field for indications that require fast resorbed membranes and a fast cell or tissue influx such as periodontal regeneration processes.

scholarly journals The Condensation of Collagen Leads to an Extended Standing Time and a Decreased Pro-inflammatory Tissue Response to a Newly Developed Pericardium-based Barrier Membrane for Guided Bone Regeneration

In Vivo ◽  
2020 ◽  
Vol 34 (3) ◽  
pp. 985-1000 ◽  
Author(s):  
TRISTAN GUELDENPFENNIG ◽  
ALIREZA HOUSHMAND ◽  
STEVO NAJMAN ◽  
SANJA STOJANOVIC ◽  
TADAS KORZINSKAS ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Tissue Response ◽  
Standing Time ◽  
Barrier Membrane ◽  
Inflammatory Tissue

scholarly journals The Early Fragmentation of a Bovine Dermis-Derived Collagen Barrier Membrane Contributes to Transmembraneous Vascularization—A Possible Paradigm Shift for Guided Bone Regeneration

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 185
Author(s):  
Eleni Kapogianni ◽  
Said Alkildani ◽  
Milena Radenkovic ◽  
Xin Xiong ◽  
Rumen Krastev ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Blood Vessels ◽  
Structural Integrity ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Collagen Membrane ◽  
Secondary Porosity ◽  
Standing Time ◽  
Barrier Membranes ◽  
Barrier Membrane

Collagen-based barrier membranes are an essential component in Guided Bone Regeneration (GBR) procedures. They act as cell-occlusive devices that should maintain a micromilieu where bone tissue can grow, which in turn provides a stable bed for prosthetic implantation. However, the standing time of collagen membranes has been a challenging area, as native membranes are often prematurely resorbed. Therefore, consolidation techniques, such as chemical cross-linking, have been used to enhance the structural integrity of the membranes, and by consequence, their standing time. However, these techniques have cytotoxic tendencies and can cause exaggerated inflammation and in turn, premature resorption, and material failures. However, tissues from different extraction sites and animals are variably cross-linked. For the present in vivo study, a new collagen membrane based on bovine dermis was extracted and compared to a commercially available porcine-sourced collagen membrane extracted from the pericardium. The membranes were implanted in Wistar rats for up to 60 days. The analyses included well-established histopathological and histomorphometrical methods, including histochemical and immunohistochemical staining procedures, to detect M1- and M2-macrophages as well as blood vessels. Initially, the results showed that both membranes remained intact up to day 30, while the bovine membrane was fragmented at day 60 with granulation tissue infiltrating the implantation beds. In contrast, the porcine membrane remained stable without signs of material-dependent inflammatory processes. Therefore, the bovine membrane showed a special integration pattern as the fragments were found to be overlapping, providing secondary porosity in combination with a transmembraneous vascularization. Altogether, the bovine membrane showed comparable results to the porcine control group in terms of biocompatibility and standing time. Moreover, blood vessels were found within the bovine membranes, which can potentially serve as an additional functionality of barrier membranes that conventional barrier membranes do not provide.

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

2010 ◽  
Vol 81 (6) ◽  
pp. 926-933 ◽  
Author(s):  
Ronaldo Barcellos de Santana ◽  
Carolina Miller Leite de Mattos ◽  
Carlos Eduardo Francischone ◽  
Thomas Van Dyke
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Tissue Response ◽  
Barrier Membrane ◽  
Soft Tissue Response

scholarly journals Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane

2020 ◽  
Vol 21 (9) ◽  
pp. 3098 ◽  
Author(s):  
Mike Barbeck ◽  
Lennart Kühnel ◽  
Frank Witte ◽  
Jens Pissarek ◽  
Clarissa Precht ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Tissue Response ◽  
Collagen Membrane ◽  
Sham Operation ◽  
Barrier Membrane ◽  
Synthetic Materials ◽  
Native Collagen

Introduction: Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations. Materials and Methods: HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods. Results: In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups. Discussion and Conclusions: HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.

In Vivo Biocompatibility of a Novel Ceramic-Metal Biocomposite

2005 ◽  
Vol 284-286 ◽  
pp. 807-810 ◽  
Author(s):  
Danyang Ying ◽  
L.X. Luo ◽  
B. Young ◽  
A.F. Julian
Keyword(s):  
Adverse Reaction ◽  
Titanium Alloy ◽  
Histological Examination ◽  
Surrounding Tissue ◽  
Subcutaneous Implantation ◽  
Control Material ◽  
Biocompatibility Test ◽  
Post Implantation

An in vivo biocompatibility test of a novel biocomposite, with major phases of CaTiO3 and Ti2O, and minor phases of AlTi3, TiO, CaO and Al2O3, was conducted on rats using subcutaneous implantation. The biocomposite and titanium alloy control specimens were removed at 6 and 14 weeks post-implantation. Histological examination revealed no significant adverse reaction of the surrounding tissue to the either the biocomposite or the control material. We conclude that the composite is well tolerated in a physiological environment.

scholarly journals Magnetic ion channel activation of TREK1 in human mesenchymal stem cells using nanoparticles promotes osteogenesis in surrounding cells

2018 ◽  
Vol 9 ◽  
pp. 204173141880869 ◽  
Author(s):  
James R Henstock ◽  
Michael Rotherham ◽  
Alicia J El Haj
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ion Channel ◽  
Human Mesenchymal Stem Cells ◽  
Host Tissue ◽  
Tissue Response ◽  
Superparamagnetic Nanoparticles ◽  
Surrounding Tissue ◽  
Channel Activation ◽  
Magnetic Ion

Magnetic ion channel activation technology uses superparamagnetic nanoparticles conjugated with targeting antibodies to apply mechanical force directly to stretch-activated ion channels on the cell surface, stimulating mechanotransduction and downstream processes. This technique has been reported to promote differentiation towards musculoskeletal cell types and enhance mineralisation. Previous studies have shown how mesenchymal stem cells injected into a pre-mineralised environment such as a foetal chick epiphysis, results in large-scale osteogenesis at the target site. However, the relative contributions of stem cells and surrounding host tissue has not been resolved, that is, are the mesenchymal stem cells solely responsible for the observed mineralisation or do mechanically stimulated mesenchymal stem cells also promote a host-tissue mineralisation response? To address this, we established a novel two-dimensional co-culture assay, which indicated that magnetic ion channel activation stimulation of human mesenchymal stem cells does not significantly promote migration but does enhance collagen deposition and mineralisation in the surrounding cells. We conclude that one of the important functions of injected human mesenchymal stem cells is to release biological factors (e.g., cytokines and microvesicles) which guide the surrounding tissue response, and that remote control of this signalling process using magnetic ion channel activation technology may be a useful way to both drive and regulate tissue regeneration and healing.

scholarly journals Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Chih Huang ◽  
Kuen-Yu Huang ◽  
Bing-Yuan Yang ◽  
Chun-Han Ko ◽  
Haw-Ming Huang
Keyword(s):  
Hyaluronic Acid ◽  
Testing Machine ◽  
Clinical Applications ◽  
Postsurgical Complications ◽  
Barrier Membrane ◽  
Universal Testing ◽  
Griess Reaction ◽  
Hydrogel Membrane ◽  
Anti Inflammatory

An antiadhesion barrier membrane is an important biomaterial for protecting tissue from postsurgical complications. However, there is room to improve these membranes. Recently, carboxymethylcellulose (CMC) incorporated with hyaluronic acid (HA) as an antiadhesion barrier membrane and drug delivery system has been reported to provide excellent tissue regeneration and biocompatibility. The aim of this study was to fabricate a novel hydrogel membrane composed of berberine-enriched CMC prepared from bark of theP. amurensetree and HA (PE-CMC/HA). In vitro anti-inflammatory properties were evaluated to determine possible clinical applications. The PE-CMC/HA membranes were fabricated by mixing PE-CMC and HA as a base with the addition of polyvinyl alcohol to form a film. Tensile strength and ultramorphology of the membrane were evaluated using a universal testing machine and scanning electron microscope, respectively. Berberine content of the membrane was confirmed using a UV-Vis spectrophotometer at a wavelength of 260 nm. Anti-inflammatory property of the membrane was measured using a Griess reaction assay. Our results showed that fabricated PE-CMC/HA releases berberine at a concentration of 660 μg/ml while optimal plasticity was obtained at a 30 : 70 PE-CMC/HA ratio. The berberine-enriched PE-CMC/HA had an inhibited 60% of inflammation stimulated by LPS. These results suggest that the PE-CMC/HA membrane fabricated in this study is a useful anti-inflammatory berberine release system.

scholarly journals Effect of Hyaluronic Acid Gel on Healing of Simple Dental Extraction Sockets: A Pilot Study

2021 ◽  
Vol 9 (D) ◽  
pp. 190-195
Author(s):  
Diana Mostafa ◽  
Maram Alzahrani ◽  
Jawharah Atiah Alatawi ◽  
Samar Farhan Alsirhani ◽  
Afrah Alshehri ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Pilot Study ◽  
Bone Repair ◽  
Healing Process ◽  
Study Groups ◽  
Adjunctive Treatment ◽  
Control Group ◽  
Wound Healing Process ◽  
Tissue Healing ◽  
Group B

BACKGROUND: Hyaluronic acid (HA) was explored as a powerful positive biocompatible material that participates in numerous biological processes related to morphogenesis and tissue healing. AIM: In this context, we elevated in this pilot study the effect of HA on soft tissue healing and bone repair of dental sockets (DS) as well as the postoperative pain. MATERIALS AND METHODS: Ten individuals of both genders (80% females and 20% males) with age range 18–44 years, were included who were diagnosed with two non-restorable teeth (total sockets n = 20). A split-mouth study design was performed where the DS of each patient were divided into Group A (study group): Topical oral HA gel (Gengigel®) was applied into sockets while Group B (control group): Sockets were left untreated. Study variables were evaluated including socket length, socket healing scores (healing index), and post-operative pain in both groups at day 1, 5, and 10 for each patient. RESULTS: There were no statistically significant differences between the control and study groups regarding the reduction of the socket length and postoperative complications but results revealed that HA enhances and fasten the healing capacity. CONCLUSION: HA can be used as adjunctive treatment to improve the wound healing process.

scholarly journals In Vivo Investigation of Soft Tissue Response of Novel Silver/Poly(Vinyl Alcohol)/ Graphene and Silver/Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Aimed for Medical Applications – The First Experience

2018 ◽  
Vol 68 (3) ◽  
pp. 321-339 ◽  
Author(s):  
Tijana Lužajić Božinovski ◽  
Danica Marković ◽  
Vera Todorović ◽  
Bogomir Prokić Bolka ◽  
Ivan Milošević ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Vinyl Alcohol ◽  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Surrounding Tissue ◽  
Poly Vinyl Alcohol ◽  
Medical Applications ◽  
Capsule Thickness ◽  
Soft Tissue Response

Abstract In this paper, we have shown for the fi rst time the soft tissue response of novel silver/ poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/ graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb©hydrogel discs, were implanted in the subcutaneous tissue of rats. Implants with the surrounding tissue were dissected after post-implantation on days 7, 15, 30 and 60, and then processed for histological examination. The tissue irritation index (TIrI) score, according to ISO 10993-6, 2007, as well as the number of leukocytes in the peri-implant zone and connective tissue capsule thickness were examined. The results show that each TIrI score, the leukocyte number around the implanted materials and capsule thickness gradually decreased during the observation period. At the endpoint of follow-up, the Ag/PVA/CHI/Gr implant was surrounded with a thinner capsule, while both the TIrI score and the number of leukocytes of the peri-implant zone were greater compared to the Ag/PVA/Gr implant. Despite the observed differences, we can conclude that our in vivo experiment suggested that both novel hydrogels were biocompatible and suitable for medical use.

scholarly journals The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

2019 ◽  
Vol 20 (8) ◽  
pp. 1969 ◽  
Author(s):  
Dominik Sieger ◽  
Tadas Korzinskas ◽  
Ole Jung ◽  
Sanja Stojanovic ◽  
Sabine Wenisch ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Substitute ◽  
Tissue Response ◽  
Control Group ◽  
L929 Cells ◽  
High Doses ◽  
Tissue Reactions ◽  
Inflammatory Macrophages

Biphasic bone substitutes (BBS) are currently well-established biomaterials. Through their constant development, even natural components like hyaluronic acid (HY) have been added to improve both their handling and also their regenerative properties. However, little knowledge exists regarding the consequences of the addition of HY to their biocompatibility and the inflammatory tissue reactions. Thus, the present study was conducted, aiming to analyze the influence of two different amounts of high molecular weight HY (HMWHY), combined with a BBS, on in vitro biocompatibility and in vivo tissue reaction. Established in vitro procedures, using L929 cells, were used for cytocompatibility analyses under the test conditions of DIN EN:ISO 10993-5. For the in vivo part of the study, calvarial defects were created in 20 Wistar rats and subsequently filled with BBS, and BBS combined with two different HMWHY amounts, i.e., BBS + HY(L) and BBS + HY(H). As controls, empty defects were used. Established histological, immunohistochemical, and histomorphometrical methods were applied to analyze the tissue reactions to the three different materials, including the induction of pro- and anti-inflammatory macrophages and multinucleated giant cells (BMGCs). The in vitro results showed that none of the materials or compositions caused biological damage to the L929 cells and can be considered to be non-toxic. The in vivo results showed that only the addition of high doses of HY to a biphasic bone substitute significantly decreases the occurrence of pro-inflammatory macrophages (* p < 0.05), comparable to the numbers found in the control group, while no significant differences within the three study groups for M2-macrophages nor BMGCs were detected. In conclusion, the addition of different amounts of HMWHY does not seem to affect the inflammation response to BBS, while improving the material handling properties.

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