Characterization of the plant-associated bacterial microbiota of the Mexican medicinal species <em>Bouvardia ternifolia</em>.

Though therapeutic compounds can be extracted directly from medicinal plant tissues; it is now known that many phytotherapeutic compounds are actually produced by associated microorganisms or due to their interaction with the plant. Bouvardia ternifolia is a medicinal plant that can be considered a potential source of therapeutic compounds, such as the antitumoral molecule bouvardin. We characterized the endophytic, endophytic+epiphyte and soil bacterial microbiota in the flower, leaves, stems and roots in specimens of this plant through 16S rDNA sequencing. We found that Proteobacteria followed by Actinobacteria were the most abundant bacteria phyla in this plant. On the other hand, the most representative genera living endophytically were Propionibacterium, Paraccocus, Lactobacillus, Kaistobacter, Methylobacterium and Erwinia. This study provides evidence of the composition and diversity of the bacterial communities present in soil and tissues of B. ternifolia which can be considered for subsequent analysis and understanding of the therapeutic compounds that give this species its medicinal properties.

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Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau

Animal Bioscience ◽

10.5713/ab.20.0809 ◽

2021 ◽

Author(s):

Xungang Wang ◽

Linyong Hu ◽

Hongjin Liu ◽

Tianwei Xu ◽

Na Zhao ◽

...

Keyword(s):

Tibetan Plateau ◽

Bacterial Microbiota ◽

Intestinal Segments

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Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions

Scientific Reports ◽

10.1038/s41598-018-30932-6 ◽

2018 ◽

Vol 8 (1) ◽

Cited By ~ 41

Author(s):

Daniel Crespo-Piazuelo ◽

Jordi Estellé ◽

Manuel Revilla ◽

Lourdes Criado-Mesas ◽

Yuliaxis Ramayo-Caldas ◽

...

Keyword(s):

Intestinal Tract ◽

Bacterial Microbiota

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Characterization of Cutaneous Bacterial Microbiota from Superficial Pyoderma Forms in Atopic Dogs

Pathogens ◽

10.3390/pathogens9080638 ◽

2020 ◽

Vol 9 (8) ◽

pp. 638

Author(s):

Caitlin E. Older ◽

Aline Rodrigues Hoffmann ◽

Kathleen Hoover ◽

Frane Banovic

Keyword(s):

Quantitative Polymerase Chain Reaction ◽

16S Rrna Gene Sequencing ◽

Molecular Techniques ◽

Rrna Gene ◽

Bacterial Microbiota ◽

Starting Point ◽

Rrna Gene Sequencing ◽

Antimicrobial Treatments ◽

Staphylococcus Spp

Although Staphylococcus pseudintermedius is considered the major pathogen associated with superficial canine pyoderma, no study has investigated the entire bacterial community in these lesions with molecular techniques. The objectives of this study were to characterize the bacterial microbiota in two forms of superficial canine pyoderma lesions, superficial bacterial folliculitis (SBF) and epidermal collarette (EC), especially in terms of the staphylococcal community. Swabs from 12 SBF and 9 EC lesions were obtained from eight and six atopic dogs, respectively. Eight samples from the axilla and groin of four healthy dogs served as controls. DNA was extracted for 16S rRNA gene sequencing and quantitative polymerase chain reaction of Staphylococcus spp. and S. pseudintermedius. Healthy skin samples harbored significantly more diverse bacterial communities than pyoderma samples. Healthy samples had communities that were more similar to each other, and were distinct from pyoderma samples. Staphylococcus spp. abundance was increased in pyoderma samples, especially those from EC samples. Although determining species-level identities of staphylococcal sequences revealed many species, S. pseudintermedius was the primary staphylococcal species found in all sample types. As expected, there are many differences in the microbiota when comparing healthy and canine pyoderma lesions samples. These lesions do not seem to be associated with a change in the relative abundance of specific Staphylococcus species, but simply an overall increase in Staphylococcus spp. abundance. The results of this study provide a starting point for future studies investigating how antimicrobial treatments may further change the microbiota associated with these lesions.

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Characterization of bacterial communities of rhizosphere and rhizoplane of Early Zhukovsky potato

E3S Web of Conferences ◽

10.1051/e3sconf/202022202050 ◽

2020 ◽

Vol 222 ◽

pp. 02050

Author(s):

Marat Lutfulin ◽

Darya Zaripova ◽

Oksana Moiseeva ◽

Semen Vologin ◽

Ayslu Mardanova

Keyword(s):

Bacterial Communities ◽

Functional Role ◽

Agricultural Crop ◽

Potato Root ◽

Bacterial Microbiota ◽

16S R Rna ◽

Significant Difference ◽

Dominant Bacteria

Identification of patterns of formation of bacterial communities of the rhizosphere and rhizoplane of potato (Solanum tuberosum L.), the most important agricultural crop, is necessary for the introduction and maintenance of sustainable organic farming. The purpose of this work was the study of the biodiversity of the bacterial microbiota of the rhizosphere and rhizoplane of Early Zhukovsky potato, cultivated on gray forest soils. Comparative analysis based on sequencing of the 16S R RNA gene showed a significant difference in the representation of different groups of bacteria in these potato root compartments. Thus, the proportions of the dominant bacteria in the rhizosphere and rhizoplane of the Proteobacteria phylum reach 47.66% ± 7.22 % and 86.35 % ± 0.53%, respectively (P < 0.05). In contrast, the representation of phylum Bacteroidetes and Firmicutes in the rhizosphere is significantly higher and reaches 41.45 % ± 10.42% and 6.49 % ± 3.23%, respectively, compared to the rhizoplane (7.84 % ± 1.24 % and 0.43 % ± 0.48 %, (P < 0.05). At the same time, Actinobacteria phylum bacteria are present in both compartments in approximately equal amounts (4.40 % ± 1.81% in the rhizosphere and 5.37 % ± 1.42% in the rhizoplane). Thus, it was found that potato forms different bacterial communities in the rhizosphere and rhizoplane in quantitative proportions, which is probably determined by the functional role of these microorganisms in the plant physiology.

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Characterization of bacterial community of rumen from bovine during laminitis challenge by high-throughput sequencing

10.21203/rs.3.rs-31535/v1 ◽

2020 ◽

Author(s):

Jian Guo ◽

Haowen Sun ◽

T. Maimai ◽

Caijun Zhao ◽

Yongguo Cao ◽

...

Keyword(s):

Lactic Acid ◽

Bacterial Community ◽

High Throughput Sequencing ◽

Economic Loss ◽

Ruminal Bacteria ◽

Classic Problem ◽

Bacterial Microbiota ◽

Clostridium Papyrosolvens ◽

Acid Metabolites

Abstract Background: Laminitis is a classic problem in the dairy industry, which can cause a great economic loss. However, the etiology and pathogenesis of laminitis have yet to be understood. In recent years, the microbiota has been the focus of much investigation in the search for various diseases. The present study aimed to explore the relationship between ruminal bacterial microbiota and laminitis. Results: The serum of healthy and laminitis bovines (n=8, respectively) collected from farms was used to detect concentrations of LPS, lactic acid, and histamine by the detection kits. This study used 16S rRNA sequencing to identify the differences in the bacterial community. The results showed that there was a significant increase in LPS and lactic acid in the laminitis group. Furthermore, microbial data analysis revealed that the laminitis group increased the abundance of bacteria with acid metabolites, such as Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009, and Erysipelotrichaceae UCG-008, and [Clostridium] papyrosolvens and Ruminococcaceae bacterium AE2021.Conclusions: In summary, the changes in ruminal bacteria may potentially serve as the risk of laminitis.

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Peer Review #1 of "Characterization of the bacterial microbiota composition and evolution at different intestinal tract in wild pigs (Sus scrofa ussuricus) (v0.1)"

10.7287/peerj.9124v0.1/reviews/1 ◽

2020 ◽

Author(s):

A Szymczak

Keyword(s):

Peer Review ◽

Sus Scrofa ◽

Intestinal Tract ◽

Microbiota Composition ◽

Wild Pigs ◽

Bacterial Microbiota

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Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

Research Society and Development ◽

10.33448/rsd-v10i15.23113 ◽

2021 ◽

Vol 10 (15) ◽

Author(s):

Nádia Elígia Nunes Pinto Paracampo ◽

Luiz Ricardo Santos de Souza ◽

Jéssica Caporal Almeida ◽

Osmar Alves Lameira

Keyword(s):

High Performance ◽

Genetic Material ◽

Chemical Characterization ◽

Array Detector ◽

Germplasm Bank ◽

Medicinal Species ◽

Breeding Programs ◽

The City ◽

Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

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