Biomass Production and Nutrient Removal by Chlorella vulgaris from Anaerobic Digestion Effluents

In this research removal of NH3-N, NO3-N and PO4-P nutrients from municipal wastewater was studied, using Chlorella vulgaris, Pseudomonas putida, Bacillus cereus and an artificial consortium of them. The objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. A model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in Chapala, Mexico. Experiments were carried out without adding an external carbon source. Results indicate that nutrient removal with Chlorella vulgaris was the most efficient with a removal of 24.03% of NO3-N, 80.62% of NH3-N and 4.30% of PO4-P. With Bacillus cereus the results were 8.40% of NO3-N, 28.80% of NH3-N and 3.80% of PO4-P. The removals with Pseudomonas putida were 2.50% of NO3-N, 41.80 of NH3-N and 4.30% of PO4-P. The consortium of Chlorella vulgaris–Bacillus cereus–Pseudomonas putida removed 29.40% of NO3-N, 4.2% of NH3-N and 8.4% of PO4-P. The highest biomass production was with Bacillus cereus (450 mg/l) followed by Pseudomonas putida (444 mg/l), the consortium (205 mg/l) and Chlorella vulgaris (88.9 mg/l). This study highlights the utility of these microorganisms for nutrient removal in wastewater treatments.

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Swine digestate treatment by prior nitrogen-starved Chlorella vulgaris: The effect of over-compensation strategy on microalgal biomass production and nutrient removal

The Science of The Total Environment ◽

10.1016/j.scitotenv.2020.144462 ◽

2021 ◽

pp. 144462

Author(s):

Chaogang Ran ◽

Xinyu Zhou ◽

Changhong Yao ◽

Yongkui Zhang ◽

Wu Kang ◽

...

Keyword(s):

Biomass Production ◽

Chlorella Vulgaris ◽

Nutrient Removal ◽

Compensation Strategy ◽

Microalgal Biomass

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Treatment and Re-Use of Raw Blackwater by Chlorella vulgaris-Based System

Water ◽

10.3390/w12102660 ◽

2020 ◽

Vol 12 (10) ◽

pp. 2660

Author(s):

Marco Antonio Segovia Bifarini ◽

Miha Žitnik ◽

Tjaša Griessler Bulc ◽

Aleksandra Krivograd Klemenčič

Keyword(s):

Biomass Production ◽

Chlorella Vulgaris ◽

Nutrient Removal ◽

Liquid Waste ◽

Oxygen Demand ◽

Biomass Productivity ◽

Dry Weight ◽

Nutrient Content ◽

Total Biomass ◽

Sanitation Systems

In this study, we examined a Chlorella vulgaris-based system as a potential solution to change liquid waste, such as blackwater, into valuable products for agriculture while protecting waters from pollution without technical demanding pre-treatment. To evaluate the possibility of nutrient removal and biomass production from raw blackwater, four blackwater dilutions were tested at lab-scale: 50%, 30%, 20%, and 10%. The results showed that even the less diluted raw blackwater was a suitable growth medium for microalgae C. vulgaris. As expected, the optimum conditions were observed in 10% blackwater with the highest growth rate (0.265 d−1) and a nutrient removal efficiency of 99.6% for ammonium and 33.7% for phosphate. However, the highest biomass productivity (5.581 mg chlorophyll-a L−1 d−1) and total biomass (332.82 mg dry weight L−1) were achieved in 50% blackwater together with the highest chemical oxygen demand removal (81%) as a result of the highest nutrient content and thus prolonged growth phase. The results suggested that the dilution factor of 0.5 followed by microalgae cultivation with a hydraulic retention time of 14 days could offer the highest biomass production for the potential use in agriculture and, in parallel, a way to treat raw blackwater from source-separation sanitation systems.

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Effect of co-substrate feeding on methane yield of anaerobic digestion of Chlorella vulgaris

Journal of Applied Phycology ◽

10.1007/s10811-016-0796-5 ◽

2016 ◽

Vol 28 (5) ◽

pp. 2741-2752 ◽

Cited By ~ 11

Author(s):

Tamás Rétfalvi ◽

Piroska Szabó ◽

Annamária-Tukacs Hájos ◽

Levente Albert ◽

Attila Kovács ◽

...

Keyword(s):

Anaerobic Digestion ◽

Chlorella Vulgaris ◽

Methane Yield ◽

Substrate Feeding

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Growth and biochemical composition of Chlorella vulgaris in different growth media

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201393312 ◽

2013 ◽

Vol 85 (4) ◽

pp. 1427-1438 ◽

Cited By ~ 49

Author(s):

MATHIAS A. CHIA ◽

ANA T. LOMBARDI ◽

MARIA DA GRACA G. MELAO

Keyword(s):

Biomass Production ◽

Chlorella Vulgaris ◽

Biochemical Composition ◽

Physiological Response ◽

Low Cost ◽

Cost Effective ◽

Growth Conditions ◽

Growth Media ◽

Continuous Cultures ◽

The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

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