scholarly journals Liquid Chromatographic/Mass Spectrometric Study on the Role of Beech (Fagus sylvatica L.) Wood Polyphenols in Red Heartwood Formation

Forests ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 10
Author(s):  
Tamás Hofmann ◽  
Roman Guran ◽  
Ondrej Zitka ◽  
Eszter Visi-Rajczi ◽  
Levente Albert
Keyword(s):  
Mass Spectrometry ◽  
Gallic Acid ◽  
Fagus Sylvatica ◽  
Syringic Acid ◽  
Heartwood Formation ◽  
Maldi Tof ◽  
Technological Defect ◽  
Fagus Sylvatica L ◽  
First Time

The present research focused on the analysis of European beech (Fagus sylvatica L.) wood polyphenols in respect to red heartwood formation, which is a significant color and technological defect of the species. For the first time, high-performance liquid chromatography/tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) were applied for the investigation of the structure and the radial distribution of polyphenols. Altogether 125 compounds were characterized by their MSn spectra, of which 71 were tentatively identified, including procyanidins (dimers to pentamers), flavonoids (taxifolin, naringenin, isorhamnetin, (epi)afzelechin), (+)-catechin, (–)-epicatechin) and their glycoside conjugates, phenolic acids (gallic acid, vanillic acid, syringic acid) and their glycosides as well as gallic acid derivatives, many of which were identified for the first time in beech wood. It was found that the concentration of many compounds increased at the color boundary. In situ polyphenol synthesis and metabolism were clearly evidenced at the color boundary. Red heartwood contained only free aglycones (syringic acid, taxifolin, naringenin, isorhamnetin, naringenin, syringic acid). Contrary to earlier assumptions, the MALDI-TOF analysis did not indicate the presence of oxidized high-molecular-weight polymeric polyphenols in the red heartwood tissues. The role of individual compounds in the formation of the red heartwood chromophores are discussed.

Synthesis of phthalocyanine compounds bearing 2-(diethoxyphosphoryl)-4-methylpenta-1,3-dienyl functional groups

2013 ◽  
Vol 17 (05) ◽  
pp. 343-350 ◽  
Author(s):  
Alexander Yu. Tolbin ◽  
Victor E. Pushkarev ◽  
Irina O. Balashova ◽  
Valery K. Brel ◽  
Yuliana I. Gudkova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Functional Groups ◽  
Spectroscopy Data ◽  
Free Base ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
First Time

Free-base phthalocyanine ligands and their zinc derivatives representing functionally monosubstituted (A3B type) or tetrasubstituted (A4 type) compounds with 2-diethoxyphosphoryl-4-methylpenta-1,3-dienyl moieties have been synthesized for the first time. Their structures were characterized by MALDI-TOF mass-spectrometry as well as 1 H and 31 P NMR spectroscopy data. A tendency to aggregation in dependence on the nature of the solvent was demonstrated as well.

scholarly journals Elucidating heterogeneity of IgA1 hinge-region O-glycosylation by use of MALDI-TOF/TOF mass spectrometry: Role of cysteine alkylation during sample processing

2013 ◽  
Vol 92 ◽  
pp. 299-312 ◽  
Author(s):  
Vojtěch Franc ◽  
Pavel Řehulka ◽  
Martin Raus ◽  
Jiří Stulík ◽  
Jan Novak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Hinge Region ◽  
Sample Processing ◽  
Maldi Tof

Surface Structures and Chemical Composition of Epicuticular Waxes during Leaf Development of Fagus sylvatica L.

1992 ◽  
Vol 47 (3-4) ◽  
pp. 190-196 ◽  
Author(s):  
P.-G. Gülz ◽  
R. B. N. Prasad ◽  
E. Müller
Keyword(s):  
Fagus Sylvatica ◽  
Coming Out ◽  
De Novo ◽  
Vegetation Period ◽  
Surface Structures ◽  
Chemical Compositions ◽  
Leaf Waxes ◽  
Fagus Sylvatica L ◽  
Chain Lengths ◽  
First Time

Abstract The surface structures of beech ( Fagus sylvatica) leaf waxes were studied by SEM and correlated with chemical compositions of the extracted wax lipids during one vegetation period. The very young leaflets just unfolding from buds contained already a wax layer without any wax sculptures or crystalloids. This wax layer is quite different in yield and composition to that of mature leaves. With the unfolding of beech leaves, a dynamic biosynthesis of several wax lipids was started, but the biosynthesis of wax esters was not continued further. Ten days after leaf unfolding the de novo biosynthesis of aldehydes could be detected for the first time. Aldehyde amount increased rapidly to about 13% of the wax. The predominant individual wax lipids synthesized were aldehydes, alcohols and fatty acids with C28 and hydrocarbons with C27 chain lengths, respectively. The biosynthesis of wax lipids in beech leaves was completed at the end of May and remained nearly constant in quantity and com position during the remaining season. At the same time when aldehydes were found for the first time, wax sculptures were observed on beech leaf waxes coming out of the continuous wax layer, exclusively on the upper leaf side. These wax sculptures increased in size and quantity in the following time and were present on the upper leaf side all over the season, only some wax sculptures show a trend to crystalline forms.

A rare case of Corynebacterium riegelii urosepsis: Role of the MALDI-TOF mass spectrometry in the identification of emerging pathogens

2019 ◽  
Vol 49 (6) ◽  
pp. 474-477
Author(s):  
M. Pichon ◽  
M. Micaelo ◽  
P. Longuet ◽  
G. Plantefève ◽  
M. Abderrahmane ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rare Case ◽  
Emerging Pathogens ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Mapping the native interaction surfaces of PREP1 with PBX1 by cross-linking mass-spectrometry and mutagenesis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chiara Bruckmann ◽  
Simone Tamburri ◽  
Valentina De Lorenzi ◽  
Nunzianna Doti ◽  
Alessandra Monti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Binding Sites ◽  
Transcriptional Activity ◽  
Tertiary Structure ◽  
Cross Linking ◽  
Interaction Sites ◽  
First Time

Abstract Both onco-suppressor PREP1 and the oncogene MEIS1 bind to PBX1. This interaction stabilizes the two proteins and allows their translocation into the nucleus and thus their transcriptional activity. Here, we have combined cross-linking mass-spectrometry and systematic mutagenesis to detail the binding geometry of the PBX1-PREP1 (and PBX1-MEIS1) complexes, under native in vivo conditions. The data confirm the existence of two distinct interaction sites within the PBC domain of PBX1 and unravel differences among the highly similar binding sites of MEIS1 and PREP1. The HR2 domain has a fundamental role in binding the PBC-B domain of PBX1 in both PREP1 and MEIS1. The HR1 domain of MEIS1, however, seem to play a less stringent role in PBX1 interaction with respect to that of PREP1. This difference is also reflected by the different binding affinity of the two proteins to PBX1. Although partial, this analysis provides for the first time some ideas on the tertiary structure of the complexes not available before. Moreover, the extensive mutagenic analysis of PREP1 identifies the role of individual hydrophobic HR1 and HR2 residues, both in vitro and in vivo.

The first synthesis of sandwich-type complex based on tetradiazepinoporphyrazine ligand

2014 ◽  
Vol 18 (01n02) ◽  
pp. 149-154 ◽  
Author(s):  
Ekaterina N. Tarakanova ◽  
Pavel A. Tarakanov ◽  
Victor E. Pushkarev ◽  
Larisa G. Tomilova
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Mass Spectrometry Data ◽  
Selective Synthesis ◽  
Maldi Tof Mass Spectrometry ◽  
H Nmr ◽  
Maldi Tof ◽  
Type Complex ◽  
Sandwich Type ◽  
First Time

Sandwich-type complex based on tetradiazepinoporphyrazine ligand — bis{tetrakis(5,7-di(4-tert-butylphenyl)-6H-1,4-diazepino)[2,3-b,g,l,q]porphyrazinato}lutetium — was synthesized for the first time. The structure of the compound has been confirmed by UV-vis/NIR, 1 H NMR spectroscopy, and MALDI-TOF mass spectrometry data. The introduction of annulated diazepine heterocycles to porphyrazine molecule significantly changes macrocycle reactivity and results in sandwich-type complex under conditions used for the selective synthesis of lanthanide(III) monophthalocyanines.

scholarly journals O-Linked Glycosylation Ensures the Normal Conformation of the Autotransporter Adhesin Involved in Diffuse Adherence

2007 ◽  
Vol 189 (24) ◽  
pp. 8880-8889 ◽  
Author(s):  
Marie-Ève Charbonneau ◽  
Victoria Girard ◽  
Anastasia Nikolakakis ◽  
Manuel Campos ◽  
Frédéric Berthiaume ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Epithelial Cells ◽  
Heat Extraction ◽  
Cell Binding ◽  
Binding Function ◽  
Cultured Epithelial Cells ◽  
First Time

ABSTRACT The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, we identified several peptides of AIDA-I modified by the addition of heptoses by use of mass spectrometry and N-terminal sequencing of proteolytic fragments of AIDA-I. One threonine and 15 serine residues were identified as bearing heptoses, thus demonstrating for the first time that AIDA-I is O-glycosylated. We observed that unglycosylated AIDA-I is expressed in smaller amounts than its glycosylated counterpart and shows extensive signs of degradation upon heat extraction. We also observed that unglycosylated AIDA-I is more sensitive to proteases and induces important extracytoplasmic stress. Lastly, as was previously shown, we noted that glycosylation is required for AIDA-I to mediate adhesion to cultured epithelial cells, but purified mature AIDA-I fused to GST was found to bind in vitro to cells whether or not it was glycosylated. Taken together, our results suggest that glycosylation is required to ensure a normal conformation of AIDA-I and may be only indirectly necessary for its cell-binding function.

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