scholarly journals Kinetic Parameters of Saccharomyces cerevisiae Alcohols Production Using Nepenthes mirabilis Pod Digestive Fluids-Mixed Agro-Waste Hydrolysates

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 10 ◽  
Author(s):  
Nkosikho Dlangamandla ◽  
Seteno Ntwampe ◽  
Justine Angadam ◽  
Boredi Chidi ◽  
Maxwell Mewa-Ngongang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Product Yield ◽  
Product Formation ◽  
Relative Difference ◽  
Substrate Utilization ◽  
Utilization Rate ◽  
Alcohol Production ◽  
Similar Product ◽  
Pre Treatment ◽  
By Products

In this study, microbial growth kinetics and modeling of alcohols production using Saccharomyces cerevisiae were evaluated using different hydrolysates in a single pot (batch) system. Mixed agro-waste hydrolysates from different pre-treatment methods, i.e., N. mirabilis/CP and HWP/DAP/CP, were used as the sole nutrient source in the fermentations used to produce the alcohols of interest. The maximum Saccharomyces cerevisiae concentration of 1.47 CFU/mL (×1010) was observed with HWP/DAP/CP hydrolysates, with a relative difference of 21.1% when compared to the N. mirabilis/CP cultures; the product yield based on biomass generation was relatively (20.2%) higher for the N. mirabilis/CP cultures. For the total residual phenolic compounds (TRPCs) generation, a relative difference (24.6%) between N. mirabilis/CP and HWP/DAP/CP pre-treatment systems was observed, suggesting that N. mirabilis/CP generates lower inhibition by-products. This was further evidenced by the lowest substrate utilization rate (3.3 × 10−4 g/(L·h)) for the N. mirabilis/CP cultures while achieving relatively similar product formation rates to those observed for the HWP/DAP/CP. A better correlation (R2 = 0.94) was obtained when predicting substrate utilization for the N. mirabilis/CP cultures. Generally, the pre-treatment of mixed agro-waste using N. mirabilis/CP seemed appropriate for producing hydrolysates which Saccharomyces cerevisiae can effectively use for alcohol production in the biorefinery industry.

A Critical Evaluation of Microbial Product Formation in Biological Processes

1987 ◽  
Vol 19 (3-4) ◽  
pp. 517-528 ◽  
Author(s):  
B. E. Rittmann ◽  
W. Bae ◽  
E. Namkung ◽  
C.-J. Lu
Keyword(s):  
Critical Evaluation ◽  
Biomass Accumulation ◽  
Anaerobic Treatment ◽  
Product Formation ◽  
Substrate Utilization ◽  
Utilization Rate ◽  
Monod Kinetics ◽  
Degradation Model ◽  
Microbial Product ◽  
Semi Empirical

A critical evaluation of the characteristics of soluble microbial products (SMP) indicates that SMP is comprised of many different types and sizes of molecules and is biodegradable. A portion of SMP is formed at a rate proportional to the rate of substrate utilization (UAP), while the rest is formed at a rate proportional to the concentration of active biomass (BAP). These characteristics are incorporated into a mathematical model that includes the following components: substrate utilization and biomass growth according to Monod kinetics, SMP formation kinetics in proportion to substrate utilization rate and to biomass accumulation, and SMP degradation according to a semi-empirical multi-component degradation model. The SMP formation/degradation model successfully describes the fractional conversion of substrate into SMP, including the observed trends of increasing conversion at high and low sludge ages. In addition to activated sludge, the model is applied to anaerobic treatment and to biofilm processes.

scholarly journals Utilization of agro-industrial by-products in Monascus fermentation: a review

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ignatius Srianta ◽  
Endang Kusdiyantini ◽  
Elok Zubaidah ◽  
Susana Ristiarini ◽  
Ira Nugerahani ◽  
...  
Keyword(s):  
Functional Food ◽  
Cost Effective ◽  
Product Yield ◽  
Food Supplements ◽  
Food Ingredients ◽  
Specific Objective ◽  
Cereal Brans ◽  
Pre Treatment ◽  
Treatment Step ◽  
By Products

AbstractThe Monascus fermentation industry has gained global attention. Its key products, i.e., pigments, functional food ingredients, food supplements, and medicinal use, are growing in the world’s market. Efforts to find the cost-effective substrate for Monascus fermentation have remained the target. This paper aimed to appraise the utilization of agro-industrial by-products (cereal, starchy tuber and root, legume, fruit, and coffee processing) as a cost-effective substrate for Monascus fermentation. The specific objective was to review the by-products pre-treatment, the fermentation process, product yield, and the bioactivity of the fermented products. Among all the by-products that could be used as the fermentation substrate, cereal brans do not need pre-treatment, but others need a suitable pre-treatment step, e.g., cassava peel, okara, and jackfruit seed to list a few, that need to be powdered beforehand. Other substrates, such as corn cob and durian seed, need soaking and size reduction through the pre-treatment step. During fermentation, Monascus produce many pigments, monacolin K, associated with rise in phenolic and flavonoid contents. These products possess antioxidant, antihypercholesterol, antidiabetes, and antiatherosclerosis activities which underpin their health significance. In conclusion, we report in this review the agro-industrial by-products which have potential prospects for pigments, functional food ingredients, food supplements, and therapeutic usages produced from Monascus fermentation.

A biometric study of higher alcohol production in Saccharomyces cerevisiae

1990 ◽  
Vol 36 (1) ◽  
pp. 61-64 ◽  
Author(s):  
Paolo Giudici ◽  
Patrizia Romano ◽  
Carlo Zambonelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Key Words ◽  
Isoamyl Alcohol ◽  
Higher Alcohols ◽  
Methionine Biosynthesis ◽  
Higher Alcohol ◽  
Strain Characteristic ◽  
Alcohol Production ◽  
Flocculation Ability ◽  
Individual Strain

A hundred strains of Saccharomyces cerevisiae were examined for the ability to produce higher alcohols. In the strains tested the production of higher alcohols was found to be an individual strain characteristic and, as such, was statistically significant. The characteristics of the strains used (flocculation ability, foaming ability, killer character, and non-H2S production) were found to be uncorrelated to isobutanol and isoamyl alcohol production, whereas the production of high levels of n-propanol was found to be related to inability to produce H2S. This, in turn, suggests a link to methionine biosynthesis. Key words: Saccharomyces cerevisiae, higher alcohols, biometry, H2S production.

scholarly journals Highly-Efficient Release of Ferulic Acid from Agro-Industrial By-Products via Enzymatic Hydrolysis with Cellulose-Degrading Enzymes: Part I–The Superiority of Hydrolytic Enzymes Versus Conventional Hydrolysis

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 782
Author(s):  
Karina Juhnevica-Radenkova ◽  
Jorens Kviesis ◽  
Diego A. Moreno ◽  
Dalija Seglina ◽  
Fernando Vallejo ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ferulic Acid ◽  
Hydrolytic Enzymes ◽  
Structural Complexity ◽  
Triticum Aestivum L ◽  
Feed Supplement ◽  
Degrading Enzymes ◽  
Secale Cereale L ◽  
Pre Treatment ◽  
By Products

Historically Triticum aestívum L. and Secale cereále L. are widely used in the production of bakery products. From the total volume of grain cultivated, roughly 85% is used for the manufacturing of flour, while the remaining part is discarded or utilized rather inefficiently. The limited value attached to bran is associated with their structural complexity, i.e., the presence of cellulose, hemicellulose, and lignin, which makes this material suitable mostly as a feed supplement, while in food production its use presents a challenge. To valorize these materials to food and pharmaceutical applications, additional pre-treatment is required. In the present study, an effective, sustainable, and eco-friendly approach to ferulic acid (FA) production was demonstrated through the biorefining process accomplished by non-starch polysaccharides degrading enzymes. Up to 11.3 and 8.6 g kg−1 of FA was released from rye and wheat bran upon 24 h enzymatic hydrolysis with multi-enzyme complex Viscozyme® L, respectively.

Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt

2021 ◽  
Vol 53 ◽  
pp. 107859
Author(s):  
Riaan den Haan ◽  
Shaunita H. Rose ◽  
Rosemary A. Cripwell ◽  
Kim M. Trollope ◽  
Marthinus W. Myburgh ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Substrate Utilization ◽  
Heterologous Production ◽  
Lessons Learnt

Integration of intestinal microbiota and metabonomic to elucidate different alleviation impacts of non-saponification and saponification astaxanthin pre-treatment on Paracetamol-induced oxidative stress in rats

2022 ◽  
Author(s):  
Ru Song ◽  
Yan Xu ◽  
Zhe Jia ◽  
Xinyan Liu ◽  
Xiaoxia Zhang
Keyword(s):  
Oxidative Stress ◽  
Intestinal Microbiota ◽  
Pre Treatment ◽  
By Products

Intestinal microbiota and metabonomic were integrated to investigate the efficiency of non-saponification or saponification astaxanthin (N-Asta or S-Asta) derived from Penaeus sinensis by-products on alleviating paracetamol (PCM)-induced oxidative stress. Pre-treated...

Inactivation, lysis and degradation by-products of Saccharomyces cerevisiae by electrooxidation using DSA

2016 ◽  
Vol 24 (7) ◽  
pp. 6096-6105 ◽  
Author(s):  
Lyliane F. Trigueiro ◽  
Larissa M. Silva ◽  
Luciana A. B. D. Itto ◽  
Thiago M. B. F. Oliveira ◽  
Artur J. Motheo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
By Products

scholarly journals Comparison of Conventional and Novel Pre-treatment Methods for Bioethanol Production from Fruit and Vegetable Wastes

2020 ◽  
Vol 33 (4) ◽  
pp. 471-483
Author(s):  
Tugba Keskin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
The Other ◽  
Yeast Fermentation ◽  
Bioethanol Production ◽  
Fruit And Vegetable ◽  
Bacterial Fermentation ◽  
Waste Gas ◽  
Clostridium Ljungdahlii ◽  
Vegetable Wastes ◽  
Pre Treatment

In this study, novel and conventional techniques for the production of bioethanol from fruit and vegetable wastes (FVWs) by yeast and bacterial fermentation were investigated experimentally. Different pretreatment techniques (acid, heat, acid/heat, and microwave) for yeast fermentation were compared. Maximum ethanol concentrations of 11.7 and 11.8 g L–1 were observed from acid/heat and microwave pretreatment, respectively, by using Saccharomyces cerevisiae. On the other hand, biochar production from FVWs and syngas fermentation from the waste gas of this process were integrated. From waste gas with 12 % CO content, 5.5 g L–1 and 2.5 g L–1 ethanol production was observed by using anaerobic mixed culture and Clostridium ljungdahlii, respectively. The overall results emphasize the potential of bioethanol production from FVWs by economically feasible and environmentally friendly methods.

scholarly journals Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation.

1996 ◽  
Vol 62 (9) ◽  
pp. 3187-3195 ◽  
Author(s):  
E Albers ◽  
C Larsson ◽  
G Lidén ◽  
C Niklasson ◽  
L Gustafsson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nitrogen Source ◽  
Product Formation ◽  
Anaerobic Growth
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