scholarly journals Production of Indole Auxins by Enterobacter sp. Strain P-36 under Submerged Conditions

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 138
Author(s):  
Francesca Luziatelli ◽  
Francesca Melini ◽  
Paolo Bonini ◽  
Valentina Melini ◽  
Veronica Cirino ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fermentation Broth ◽  
Operating Mode ◽  
Inoculum Size ◽  
Plant Growth Promoting Bacteria ◽  
Fermentation Time ◽  
Auxin Production ◽  
Generation Plant ◽  
Shake Flask Cultivation ◽  
Plant Growth Promoting

Bioactive compounds produced by plant growth-promoting bacteria through a fermentation process can be valuable for developing innovative second-generation plant biostimulants. The purpose of this study is to investigate the biotechnological potential of Enterobacter on the production of auxin—a hormone with multiple roles in plant growth and development. The experiments were carried in Erlenmeyer flasks and a 2-L fermenter under batch operating mode. The auxin production by Enterobacter sp. strain P-36 can be doubled by replacing casein with vegetable peptone in the culture medium. Cultivation of strain P36 in the benchtop fermenter indicates that by increasing the inoculum size 2-fold, it is possible to reduce the fermentation time from 72 (shake flask cultivation) to 24 h (bioreactor cultivation) and increase the auxin volumetric productivity from 6.4 to 17.2 mg [IAAequ]/L/h. Finally, an efficient storage procedure to preserve the bacterial auxin was developed. It is noteworthy that by sterilizing the clarified fermentation broth by filtration and storing the filtrated samples at +4 °C, the level of auxin remains unchanged for at least three months.

Auxin-producing Bacillus sp.: Auxin quantification and effect on the growth of Solanum tuberosum

2010 ◽  
Vol 82 (1) ◽  
pp. 313-319 ◽  
Author(s):  
Ambreen Ahmed ◽  
Shahida Hasnain
Keyword(s):  
Solanum Tuberosum ◽  
Plant Growth ◽  
High Performance ◽  
Growth Parameters ◽  
Plant Growth Promoting Bacteria ◽  
Chromatography Analysis ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Tryptophan Concentration ◽  
Growth Promoting

Plant-associated bacteria are known to improve plant growth and play a major role in the development of plants. The present study is concerned with the isolation of two auxin-producing plant growth-promoting bacteria (PGPB). On the basis of 16S rRNA sequencing, both of the strains are identified as Bacillus sp. Maximum auxin production was observed at 37 °C after 48 h of incubation. Increase in tryptophan concentration stimulated auxin production by the isolates. High-performance liquid chromatography analysis showed that the bacterial auxin exhibited similar retention time as the standard indole-3-acetic acid (IAA). Sprouts of Solanum tuberosum var. Desiree were inoculated with the isolates. Comparison of various growth parameters of inoculated plants with non-inoculated plants revealed the improvement of plant growth by bacterial inoculation. Almost 40 and 35 % increase in shoot length with P4 and S6 inoculation, respectively, was observed. Considerable improvement in root growth was observed with an increase in the number and length of roots. On the basis of the above findings, it is concluded that the plant growth-promoting Bacillus strains affect S. tuberosum beneficially, resulting in improved plant growth.

scholarly journals Global RNA sequencing reveals enhanced photosynthetic activity and auxin response in Brassica napus treated with Pseudomonas chlororaphis PA23

2020 ◽  
Author(s):  
Mark F. Belmonte ◽  
Teresa R de Kievit ◽  
Joey C Wan ◽  
Ayo Bolaji ◽  
Emma Gray ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Plant Growth ◽  
Rna Sequencing ◽  
Plant Growth Promoting Bacteria ◽  
Pseudomonas Chlororaphis ◽  
Phenotypic Differences ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting bacteria (PGPB) are a growing subset of agricultural adjuncts which can be used to increase crop yield and plant productivity. Although substantial research has been conducted on the metabolites and active molecules secreted by PGPBs, relatively little is known about their effects on the global transcriptome of the host plant. The present study was carried out to investigate changes in the gene expression landscape of early vegetative Brassica napus following treatment with Pseudomonas chlororaphis PA23. This PGPB was isolated from the soybean rhizosphere and has been extensively studied as a biocontrol agent. However, little is known about its effects on plant growth and development. Using a combination of RNA-sequencing and physiological analyses, we identified increased abundance of mRNA transcripts associated with photosynthesis and phytohormone response. Phenotypically we observed increased photosynthetic rates and larger root and shoot systems in B. napus following P. chlororaphis PA23 treatment. Lastly, we identified auxin production by P. chlororaphis PA23 which likely contributes to changes in gene expression and the observed phenotypic differences in root and shoot structures. Together, the results of our study suggest that PA23 is a potent plant growth promoting agent with the potential for field applications as an agricultural adjunct.

Enhancing salt tolerance in quinoa by halotolerant bacterial inoculation

2016 ◽  
Vol 43 (7) ◽  
pp. 632 ◽  
Author(s):  
Aizheng Yang ◽  
Saqib Saleem Akhtar ◽  
Shahid Iqbal ◽  
Muhammad Amjad ◽  
Muhammad Naveed ◽  
...  
Keyword(s):  
Plant Growth ◽  
Saline Water ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Saline Irrigation ◽  
Auxin Production ◽  
Plant Growth Promoting ◽  
Growth Promoting

Quinoa is a facultative halophytic seed crop of increasing interest worldwide. Its performance declines under high salinity but can be improved by using halotolerant plant growth-promoting bacteria (PGPB) containing multi-traits, i.e. ACC-deaminase activity, exopolysaccharide secretion and auxin production. This study focussed on improving the productivity of quinoa through the use of six plant growth-promoting bacterial strains (both endophytic and rhizosphere). These were screened by conducting osmoadaptation assay, and the two most halotolerant strains (Enterobacter sp. (MN17) and Bacillus sp. (MN54)) were selected. These two strains were evaluated for their effects on growth, physiological characters and yield of quinoa. At the five leaf stage plants were irrigated with saline water having either 0 or 400 mM NaCl. The results indicated that saline irrigation significantly decreased the growth of quinoa, whereas inoculation of plants with MN17 and MN54 mitigated the negative effects of salinity by improving plant water relations and decreasing Na+ uptake, which consequently, reduced osmotic and ionic stress. Strain MN54 performed better than MN17, which might be because of its better growth promoting traits and higher rhizosphere colonisation efficiency than MN17. Our results suggest that growth and productivity of quinoa could be improved by inoculating with highly tolerant PGPB strain in salt-affected soils.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

Effect of plant growth promoting bacteria and drought on spring maize (Zea mays L.)

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Muhammad Mubeen ◽  
Asghari Bano ◽  
Barkat Ali ◽  
Zia Ul Islam ◽  
Ashfaq Ahmad ◽  
...  
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Zea Mays L ◽  
Plant Growth Promoting Bacteria ◽  
Spring Maize ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant Growth Promoting Bacteria (PGPB) - A Versatile Tool for Plant Health Management

2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Salah Eddin Khabbaz ◽  
D. Ladhalakshmi ◽  
Merin Babu ◽  
A. Kandan ◽  
V. Ramamoorthy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Health Management ◽  
Plant Health ◽  
Plant Growth Promoting Bacteria ◽  
Versatile Tool ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

scholarly journals Genome Insights into the Novel Species Jejubacter calystegiae, a Plant Growth-Promoting Bacterium in Saline Conditions

Diversity ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 24
Author(s):  
Ling Min Jiang ◽  
Yong Jae Lee ◽  
Ho Le Han ◽  
Myoung Hui Lee ◽  
Jae Cheol Jeong ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Novel Species ◽  
Endophytic Bacterium ◽  
Morning Glory ◽  
Plant Growth Promoting Bacteria ◽  
Illumina Hiseq ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Sequencing Platforms

Jejubacter calystegiae KSNA2T, a moderately halophilic, endophytic bacterium isolated from beach morning glory (Calystegia soldanella), was determined to be a novel species in a new genus in the family Enterobacteriaceae. To gain insights into the genetic basis of the salinity stress response of strain KSNA2T, we sequenced its genome using two complementary sequencing platforms (Illumina HiSeq and PacBio RSII). The genome contains a repertoire of metabolic pathways, such as those for nitrogen, phosphorus, and some amino acid metabolism pathways. Functional annotation of the KSNA2T genome revealed several genes involved in salt tolerance pathways, such as those encoding sodium transporters, potassium transporters, and osmoprotectant enzymes. Plant growth-promoting bacteria-based experiments indicated that strain KSNA2T promotes the germination of vegetable seeds in saline conditions. Overall, the genetic and biological analyses of strain KSNA2T provide valuable insights into bacteria-mediated salt tolerance in agriculture.

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