scholarly journals Modeling and Use of Inter-Criteria Decision Analysis for Selecting Growth Rate Models for Batch Cultivation of Yeast Kluyveromyces marxianus var. lactis MC 5

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 163
Author(s):  
Mitko Petrov
Keyword(s):  
Growth Rate ◽  
Decision Analysis ◽  
Specific Growth ◽  
Batch Cultivation ◽  
Process Models ◽  
Monod Model ◽  
First Time ◽  
Kinetics Of ◽  
Additive Form ◽  
Selection Of

Ten unstructured models of Monod, Mink, Tessier, Moser, Aiba, Andrews, Haldane, Luong, Edward, and Han-Levenspiel are considered in this paper to explain the kinetics of cell growth for batch cultivation of the yeast Kluyweromyces marxianus var. lactis MC 5. For the first time, two independent kinetic models are used to model the process for the two basic substrates—lactose and oxygen. The selection of the most appropriate growth rate models has been made through a new multi-criteria decision-making approach called the Inter-Criteria Decision Analysis (ICDA) method. The application of ICDA to the growth rate of lactose and oxygen alone has shown that there have been many correlations between the studied models. Thus, the models for the growth rate, depending only on lactose, are reduced to one—Monod model and there are two models—Monod and Mink—depending on oxygen only. Separate kinetic process models have been developed for the combination of Monod–Monod and Monod–Mink models. For the first time, in addition to the multiplicative form, the additive form of a specific growth rate has been studied. The comparison of the obtained results has shown that the additive form has shown better results than the multiplicative one. For this reason, the additive form of the Monod–Monod model will be used to model the process.

Growth kinetics of Escherichia coli with galactose and several other sugars in carbon-limited chemostat culture

1999 ◽  
Vol 46 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Urs Lendenmann ◽  
Mario Snozzi ◽  
Thomas Egli
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Steady State ◽  
Specific Growth Rate ◽  
Growth Kinetics ◽  
Specific Growth ◽  
Chemostat Culture ◽  
Monod Model ◽  
E Coli ◽  
Kinetics Of

Kinetic models for microbial growth describe the specific growth rate (μ) as a function of the concentration of the growth-limiting nutrient (s) and a set of parameters. A typical example is the model proposed by Monod, where μ is related to s using substrate affinity (Ks) and the maximum specific growth rate (μmax). The preferred method to determine such parameters is to grow microorganisms in continuous culture and to measure the concentration of the growth-limiting substrate as a function of the dilution rate. However, owing to the lack of analytical methods to quantify sugars in the microgram per litre range, it has not been possible to investigate the growth kinetics of Escherichia coli in chemostat culture. Using an HPLC method able to determine steady-state concentrations of reducing sugars, we previously have shown that the Monod model adequately describes glucose-limited growth of E. coli ML30. This has not been confirmed for any other sugar. Therefore, we carried out a similar study with galactose and found steady-state concentrations between 18 and 840 μg·L-1 for dilution rates between 0.2 and 0.8·h-1, respectively. With these data the parameters of several models giving the specific growth rate as a function of the substrate concentration were estimated by nonlinear parameter estimation, and subsequently, the models were evaluated statistically. From all equations tested, the Monod model described the data best. The parameters for galactose utilisation were μmax = 0.75·h-1 and Ks = 67 μg·L-1. The results indicated that accurate Ks values can be estimated from a limited set of steady-state data when employing μmax measured during balanced growth in batch culture. This simplified procedure was applied for maltose, ribose, and fructose. For growth of E. coli with these sugars, μmax and Ks were for maltose 0.87·h-1, 100 μg·L-1; for ribose 0.57·h-1, 132 μg·L-1, and for fructose 0.70·h-1, 125 μg·L-1. Key words: monod model, continuous culture, galactose, glucose, fructose, maltose, ribose.

scholarly journals Modelling and Multi-Criteria Decision Making for Selection of Specific Growth Rate Models of Batch Cultivation by Saccharomyces cerevisiae Yeast for Ethanol Production

Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 61 ◽  
Author(s):  
Petrov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Decision Making ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Batch Cultivation ◽  
Preference Ranking ◽  
Multi Criteria Decision Making ◽  
Promethee Ii ◽  
Selection Of

This study is focused on using multi-criteria decision making (MCDM) for selecting specific growth rate models of batch cultivation by the Saccharomyces cerevisiae. Ten specific growth rate models—Monod, Mink, Tessier, Moser, Aiba, Andrews, Haldane, Luong, Edward, and Han-Levenspiel—were investigated in order to explain the cell growth kinetics by the dependence on glucose. By using the preference ranking organization method (PROMETHEE) II, it was found that the Andrews model was the highest of rank and was the most appropriate one for modelling.

scholarly journals AKTIVITAS INULINASE OLEH Pichia manshurica DAN FUSAN F4 PADA FERMENTASI BATCH DENGAN UMBI DAHLIA (Dahlia sp) SEBAGAI SUBSTRAT

REAKTOR ◽  
2015 ◽  
Vol 14 (3) ◽  
pp. 187 ◽  
Author(s):  
Wijanarka Wijanarka ◽  
Endang Sutariningsih Soetarto ◽  
Kumala Dewi ◽  
Ari Indrianto
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Lag Phase ◽  
Growth Media ◽  
A Value ◽  
Inulinase Activity ◽  
Pichia Manshurica ◽  
Microbial Strains ◽  
Kinetics Of

ACTIVITY OF INULINASE OF Pichia Manshuria AND FUSAN F4 ON BATCH FERMENTATION UDING DAHLIA TUBER (Dahlia sp) AS A SUBSTRATE. A dahlia tuber is one of the common inulin rich crops. Inulin is formed by units of fructans, which are polymers of D-fructose. Inulinases (EC 3.2.1.7) catalyze the hydrolysis of inulin, producing fructooligosaccharides (FOS), inulooligosaccharides (IOS), pulullan, acetone, butanol and sorbitol, therefore dahlia tubers are used as growth media. The inulin hydrolyzing activity has been reported from various microbial strains Pichia manshurica and Fusan F4 which is the result of fusion protoplast. The objective of this study was to determine the activity of inulinase Pichia manshurica and Fusan F4 on the substrate dahlia tubers. Fusan F4 to increase inulinase activity compared with Pichia manshurica and to investigate the kinetics of specific growth rate (μ) and time double (g) from of Pichia manshurica and Fusan F4. The results showed that the exponential phase occurs at 0-12 hour without a lag phase. P. manshurica has a specific growth rate (μ) of 0.18/hour with time double (g) 3.90 hours and the inulinase enzyme activity of 0.56 IU, while for Fusan F4 consecutive has a value μ of 0.20/hour, g of 3.49 hours and the activity of 0.69 IU. The conclusion of this research is to improve Fusan F4 inulinase activity and the ability has to be better than the Pichia manshurica.Umbi dahlia merupakan salah satu umbi yang mengandung inulin. Inulin merupakan polimer fruktan yang dapat dipecah oleh enzim inulinase (E.C. 3.2.1.7) menjadi fruktosa. Fruktosa merupakan bahan baku dasar untuk pembuatan FOS, IOS, pulullan, aseton dan sorbitol, oleh karena itu umbi dahlia digunakan sebagai media pertumbuhan. Enzim inulinase ini secara indigenous dimiliki oleh Pichia manshurica dan Fusan F4 yang merupakan hasil fusi protoplas.Tujuan  penelitian ini adalah  untuk mengetahui aktivitas inulinase Pichia manshurica dan Fusan F4 pada substrat umbi dahlia, Fusan F4 mampu meningkatkan aktivitas inulinase dibandingkan dengan Pichia manshurica serta untuk mengetahui kinetika kecepatan pertumbuhan specifik (µ) dan waktu generasi (g) Pichia manshurica dan Fusan F4. Hasil penelitian menunjukkan bahwa fase  eksponensial terjadi pada jam ke-0 sampai jam ke-12 tanpa diikuti fase lag, Pichia manshurica mempunyai kecepatan pertumbuhan specific (µ)  sebesar 0,18/jam dengan waktu generasi (g) 3,90 jam dan aktivitas enzim inulinase yang dihasilkan sebesar 0,56 IU, sedangkan untuk fusan F4 secara berturut-turut mempunyai nilai µ sebesar 0,20/jam, g sebesar 3,49 jam dan aktivitas sebesar 0,69 IU. Kesimpulan dari penelitian ini adalah Fusan F4 mampu meningkatkan aktivitas inulinase dan mempunyai kemampuan lebih baik dibanding dengan Pichia manshurica.

The kinetics of growth control in a colonial hydroid

1981 ◽  
Vol 61 (1) ◽  
pp. 35-63 ◽  
Author(s):  
A. R. D. Stebbing
Keyword(s):  
Growth Rate ◽  
Growth Process ◽  
Control Mechanism ◽  
Specific Growth ◽  
Growth Control ◽  
Temporal Variations ◽  
Controlled Experiments ◽  
Rates Of Growth ◽  
Kinetics Of ◽  
Colonial Hydroid

It is suggested that the cumulative view of growth in which some index of biomass is plotted against time tends to obscure temporal variations in the growth process that might provide evidence of how it is controlled. Experiments with the colonial hydroid Campanularia flexuosa show that the action of a growth control mechanism can be demonstrated by considering changes in specific rates of growth determined at frequent intervals in time. However, it is also necessary to disturb the growth process slightly in order to initiate the action of the control mechanism, and having done so, to isolate the effect of the disturbance on growth and thus the action of the control mechanism. This is done by expressing the specific growth rate of organisms whose growth is disturbed as a percentage of that of control organisms of the same age.

Vitamin B12 and Marine Ecology. IV. The Kinetics of Uptake, Growth and Inhibition in Monochrysis Lutheri

1968 ◽  
Vol 48 (3) ◽  
pp. 689-733 ◽  
Author(s):  
M. R. Droop
Keyword(s):  
Growth Rate ◽  
Substrate Concentration ◽  
Specific Growth ◽  
Marine Ecology ◽  
Vitamin B ◽  
Batch Cultures ◽  
Washed Cell ◽  
Cell Quota ◽  
Kinetics Of Uptake ◽  
Kinetics Of

57Co-labelled vitamin B12 was used to study the kinetics of vitamin B12 limitation in Monochrysis lutheri in continuous and exponentially growing batch cultures and in washed cell suspensions.Specific growth rate in the chemostats was found not to depend directly on medium substrate concentration but on the concentration within the cells (cell quota), obeying a hyperbolic equation of the form

scholarly journals Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Javier Garrigós-Martínez ◽  
Miguel Angel Nieto-Taype ◽  
Arnau Gasset-Franch ◽  
José Luis Montesinos-Seguí ◽  
Xavier Garcia-Ortega ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Pichia Pastoris ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Gene Dosage ◽  
Fed Batch ◽  
Komagataella Phaffii ◽  
Production Kinetics ◽  
Kinetics Of

Abstract Background The PAOX1-based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of specific growth rate (µ) in AOX1 regulation remains unknown, and therefore, its impact on protein production kinetics is still unclear. Results The influence of heterologous gene dosage, and both, operational mode and strategy, on culture physiological state was studied by cultivating the two PAOX1-driven Candida rugosa lipase 1 (Crl1) producer clones. Specifically, a clone integrating a single expression cassette of CRL1 was compared with one containing three cassettes over broad dilution rate and µ ranges in both chemostat and fed-batch cultivations. Chemostat cultivations allowed to establish the impact of µ on the MUT-related MIT1 pool which leads to a bell-shaped relationship between µ and PAOX1-driven gene expression, influencing directly Crl1 production kinetics. Also, chemostat and fed-batch cultivations exposed the favorable effects of increasing the CRL1 gene dosage (up to 2.4 fold in qp) on Crl1 production with no significant detrimental effects on physiological capabilities. Conclusions PAOX1-driven gene expression and Crl1 production kinetics in P. pastoris were successfully correlated with µ. In fact, µ governs MUT-related MIT1 amount that triggers PAOX1-driven gene expression—heterologous genes included—, thus directly influencing the production kinetics of recombinant protein.

scholarly journals Kinetika detoksifikasi umbi gadung (dioscorea hispida dennst.) secara fermentasi dengan kapang mucor racemosus

REAKTOR ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 81 ◽  
Author(s):  
Marissa Widiyanti ◽  
Andri Cahyo Kumoro
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Cyanogenic Glucosides ◽  
Mucor Racemosus ◽  
Dry Land ◽  
Physical Chemical ◽  
Fungal Fermentation ◽  
Biological Methods ◽  
Kinetics Of

Abstract THE KINETICS OF GADUNG (Dioscorea hispida dennst.) TUBER DETOXIFICATION VIA FUNGAL FERMENTATION USING Mucor racemosus. Bitter yam (Dioscorea hispida Dennst.) is one of carbohydrate sources used as staple food commonly found in Indonesian dry-land. However, this tuber has been underutilized due to the presence of an antinutrition compound, namely cyanogenic glucosides. Removal of cyanides from foodstuffs can be done either by physical, chemical or biological methods. In this study, the effect of time on the detoxification of gadung tuber chips from cyanides via fermentation using Mucor racemosus and its kinetics were investigated. Gadung chip samples were withdrawn from the fermentation system at every 24 hours interval for biomass and cyanides contents analysis. It was clear that the cyanides content decreased as the fermentation went by. Safely consumed gadung tuber chips were obtained from fermentation of the chips for 120 hours from which cyanides content as low as 49.41 mg/kg was achieved. The logistic equation successfully described the growth rate of Mucor racemosus under studied condition. The specific growth rate of Mucor racemosus in gadung chips was found to be 0.0297/hr or about a half of specific growth rate of that mold when grown in the readily consumed yeast-pepton-glucose (YPG) media. Keywords: fermentation; yam; monod; Mucor racemosus; cyanogen  Abstrak Umbi gadung (Dioscorea hispida Dennst.) merupakan salah satu sumber karbohidrat yang digunakan sebagai makanan pokok yang biasa ditemukan di lahan kering di wilayah Indonesia. Namun, umbi ini kurang dimanfaatkan karena adanya senyawa antinutrisi, yaitu cyanogenic glucosides. Penghilangan senyawa sianida dari bahan makanan dapat dilakukan baik dengan metode fisik, kimia atau biologi. Penelitian ini bertujuan untuk mengkaji pengaruh waktu pada detoksifikasi irisan umbi gadung dari senyawa sianida melalui fermentasi dengan menggunakan kapang Mucor racemosus dan kinetikanya. Cuplikan irisan umbi gadung diambil dari sistem fermentasi pada setiap jeda waktu 24 jam untuk dianalisis kadar biomassa dan sianidanya. Hasil penelitian menunjukkan bahwa kandungan sianida menurun seiring dengan bertambahnya waktu fermentasi. Irisan umbi gadung yang aman dikonsumsi dapat diperoleh dari fermentasi irisan ubi gadung selama 120 jam dengan kandungan sianida serendah 49,41 mg/kg. Persamaan logistik berhasil menggambarkan tingkat pertumbuhan kapang Mucor racemosus dengan baik. Tingkat pertumbuhan spesifik dari Mucor racemosus pada irisan umbi gadung adalah 0,0297/jam atau sekitar setengah dari laju pertumbuhan spesifik jamur tersebut saat dibudidayakan pada media yang siap dikonsumsi, seperti ragi-pepton-glukosa (YPG). Kata kunci: fermentasi; gadung; monod; Mucor racemosus; sianogen 

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