scholarly journals In Vitro Activity of Selected Phenolic Compounds against Planktonic and Biofilm Cells of Food-Contaminating Yeasts

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1652
Author(s):  
Bernard Gitura Kimani ◽  
Erika Beáta Kerekes ◽  
Csilla Szebenyi ◽  
Judit Krisch ◽  
Csaba Vágvölgyi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Cinnamic Acid ◽  
Debaryomyces Hansenii ◽  
Pichia Anomala ◽  
Growth Inhibitors ◽  
Biofilm Growth ◽  
Natural Substances ◽  
Spoilage Yeasts ◽  
Potent Growth

Phenolic compounds are natural substances that can be obtained from plants. Many of them are potent growth inhibitors of foodborne pathogenic microorganisms, however, phenolic activities against spoilage yeasts are rarely studied. In this study, planktonic and biofilm growth, and the adhesion capacity of Pichia anomala, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Debaryomyces hansenii spoilage yeasts were investigated in the presence of hydroxybenzoic acid, hydroxycinnamic acid, stilbene, flavonoid and phenolic aldehyde compounds. The results showed significant anti-yeast properties for many phenolics. Among the tested molecules, cinnamic acid and vanillin exhibited the highest antimicrobial activity with minimum inhibitory concentration (MIC) values from 500 µg/mL to 2 mg/mL. Quercetin, (−)-epicatechin, resveratrol, 4-hydroxybenzaldehyde, p-coumaric acid and ferulic acid were also efficient growth inhibitors for certain yeasts with a MIC of 2 mg/mL. The D. hansenii, P. anomala and S. pombe biofilms were the most sensitive to the phenolics, while the S. cerevisiae biofilm was quite resistant against the activity of the compounds. Fluorescence microscopy revealed disrupted biofilm matrix on glass surfaces in the presence of certain phenolics. Highest antiadhesion activity was registered for cinnamic acid with inhibition effects between 48% and 91%. The active phenolics can be natural interventions against food-contaminating yeasts in future preservative developments.

scholarly journals Decarboxylation of Sorbic Acid by Spoilage Yeasts Is Associated with the PAD1 Gene

2007 ◽  
Vol 73 (20) ◽  
pp. 6534-6542 ◽  
Author(s):  
Malcolm Stratford ◽  
Andrew Plumridge ◽  
David B. Archer
Keyword(s):  
Carboxylic Acids ◽  
Cinnamic Acid ◽  
Sorbic Acid ◽  
Debaryomyces Hansenii ◽  
Pichia Anomala ◽  
Gas Chromatography Mass Spectrometry ◽  
Cinnamic Acids ◽  
Mechanism Of Resistance ◽  
Spoilage Yeast ◽  
Spoilage Yeasts

ABSTRACT The spoilage yeast Saccharomyces cerevisiae degraded the food preservative sorbic acid (2,4-hexadienoic acid) to a volatile hydrocarbon, identified by gas chromatography mass spectrometry as 1,3-pentadiene. The gene responsible was identified as PAD1, previously associated with the decarboxylation of the aromatic carboxylic acids cinnamic acid, ferulic acid, and coumaric acid to styrene, 4-vinylguaiacol, and 4-vinylphenol, respectively. The loss of PAD1 resulted in the simultaneous loss of decarboxylation activity against both sorbic and cinnamic acids. Pad1p is therefore an unusual decarboxylase capable of accepting both aromatic and aliphatic carboxylic acids as substrates. All members of the Saccharomyces genus (sensu stricto) were found to decarboxylate both sorbic and cinnamic acids. PAD1 homologues and decarboxylation activity were found also in Candida albicans, Candida dubliniensis, Debaryomyces hansenii, and Pichia anomala. The decarboxylation of sorbic acid was assessed as a possible mechanism of resistance in spoilage yeasts. The decarboxylation of either sorbic or cinnamic acid was not detected for Zygosaccharomyces, Kazachstania (Saccharomyces sensu lato), Zygotorulaspora, or Torulaspora, the genera containing the most notorious spoilage yeasts. Scatter plots showed no correlation between the extent of sorbic acid decarboxylation and resistance to sorbic acid in spoilage yeasts. Inhibitory concentrations of sorbic acid were almost identical for S. cerevisiae wild-type and Δpad1 strains. We concluded that Pad1p-mediated sorbic acid decarboxylation did not constitute a significant mechanism of resistance to weak-acid preservatives by spoilage yeasts, even if the decarboxylation contributed to spoilage through the generation of unpleasant odors.

scholarly journals Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

2020 ◽  
Vol 10 (5) ◽  
pp. 1845 ◽  
Author(s):  
Alexandra M. Afonso ◽  
Joana Gonçalves ◽  
Ângelo Luís ◽  
Eugenia Gallardo ◽  
Ana Paula Duarte
Keyword(s):  
Wound Healing ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Natural Substances ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Assay ◽  
Wound Healing Activity

Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.

Clotrimazole, Ketoconazole, and Clodinafop-propargyl as Potent Growth Inhibitors of Equine Babesia Parasites During In Vitro Culture

2003 ◽  
Vol 89 (3) ◽  
pp. 604-606 ◽  
Author(s):  
Sabine Bork ◽  
Naoaki Yokoyama ◽  
Tomohide Matsuo ◽  
Florencia G. Claveria ◽  
Kozo Fujisaki ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Growth Inhibitors ◽  
Potent Growth ◽  
Clodinafop Propargyl

scholarly journals Synthesis of Hydrazone Derivatives of 4-[4-Formyl-3-(2-oxochromen-3-yl)pyrazol-1-yl]benzoic acid as Potent Growth Inhibitors of Antibiotic-resistant Staphylococcus aureus and Acinetobacter baumannii

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2051 ◽  
Author(s):  
Jedidiah Whitt ◽  
Cameron Duke ◽  
Anthony Sumlin ◽  
Steven A. Chambers ◽  
Rawan Alnufaie ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Growth Inhibitors ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Antimicrobial Studies ◽  
New Antibiotics ◽  
Global Concern ◽  
Potent Growth

Microbial resistance to drugs is an unresolved global concern, which is present in every country. Developing new antibiotics is one of the guidelines of the Centers for Disease Control and Preventions (CDC) to combat bacterial resistance to drugs. Based on our lead molecules, we report the synthesis and antimicrobial studies of 27 new pyrazole derivatives. These new coumarin-pyrazole-hydrazone hybrids are readily synthesized from commercially available starting materials and reagents using benign reaction conditions. All the synthesized molecules were tested against 14 Gram-positive and Gram-negative bacterial strains. Several of these molecules have been found to be potent growth inhibitors of several strains of these tested bacteria with minimum inhibitory concentrations as low as 1.56 μg/mL. Furthermore, active molecules are non-toxic in in vitro and in vivo toxicity studies.

scholarly journals The Bioactive Potential of Functional Products and Bioavailability of Phenolic Compounds

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 953 ◽  
Author(s):  
Cristina Monica Dabulici ◽  
Ionela Sârbu ◽  
Emanuel Vamanu
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Saccharomyces Boulardii ◽  
Ilex Paraguariensis ◽  
Syzygium Aromaticum ◽  
Natural Substances ◽  
Assimilation Capacity

The expression of bioactivity depends on the assimilation of different classes of natural substances (e.g., phenolic compounds) in vivo. Six functional extracts (Aspalathus linearis, leaves; Paullinia cupana, seeds; Aristotelia chilensis, berries; Ilex paraguariensis, leaves; Syzygium aromaticum, cloves, and wild berries) were analyzed in vitro and in vivo as an alternative to alleviating pathologies associated with oxidative stress (proliferation of cancer cells). The purpose of this research was to evaluate the in vitro and in vivo antioxidant and cytotoxic potential of hydroalcoholic solutions, in addition to the assimilation capacity of bioactive components in Saccharomyces boulardii cells. In vivo antioxidant capacity (critical point value) was correlated with the assimilation ratio of functional compounds. The results of in vitro antioxidant activities were correlated with the presence of quercetin (4.67 ± 0.27 mg/100 mL) and chlorogenic acid (14.38 ± 0.29 mg/100 mL) in I. paraguariensis. Bioassimilation of the main nutraceutical components depended on the individual sample. Phenolic acid levels revealed the poor assimilation of the main components, which could be associated with cell viability to oxidative stress.

Phenolic compounds from in vitro cultures of Rindera graeca Boiss. & Feldr.

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
K Graikou ◽  
H Damianakos ◽  
M Jeziorek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Cultures

Inhibition by Phenol and Phenolic Acids of Platelet Release Reaction

1973 ◽  
Vol 30 (02) ◽  
pp. 334-338 ◽  
Author(s):  
Felisa C. Molinas
Keyword(s):  
Renal Failure ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Platelet Function ◽  
Deleterious Effect ◽  
Release Reaction ◽  
Contributory Factors ◽  
Adp Release ◽  
Platelet Release

SummaryIt has been postulated that the high phenol and phenolic acids plasmatic levels found in patients with chronic renal failure are contributory factors in the abnormal platelet function described in these patients. This hypothesis was corroborated by “in vitro” studies showing the deleterious effect of these compounds on certain platelet function after pre-incubation of PRP with phenol and phenolic compounds. The present studies were conducted to determine the influence of phenolic compounds on platelet release reaction. It was found that phenol inhibited from 62.5 to 100% the effect of the aggregating agents thrombin, adrenaline and ADP on platelet 5-HT-14C release. The phenolic acids p-, m-, and o-HPAA inhibited from 36.35 to 94.8% adrenaline and ADP-induced platelet 5-HT-14C release. Adrenaline-induced platelet ADP release was inhibited from 27.45 to 38.10% by the phenolic compounds. These findings confirm the hypothesis that phenolic compounds interfere with platelet function through the inhibition of the release reaction.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

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