scholarly journals Potential Activity of Abrantes Pollen Extract: Biochemical and Cellular Model Studies

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2804
Author(s):  
Ana C. Gonçalves ◽  
Radhia Aitfella Lahlou ◽  
Gilberto Alves ◽  
Cristina Garcia-Viguera ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
High Performance ◽  
Human Erythrocytes ◽  
Pollen Extract ◽  
Ionization Mass ◽  
Pharmaceutical Drugs ◽  
Photodiode Array Detection ◽  
Potential Health ◽  
Quercetin Derivatives ◽  
Biological Potential

The aim of this study was to determine the grain composition and (poly)phenolic profile of pollen from Abrantes (Portugal), as well as its antioxidative and antidiabetic properties, and abilities to protect human erythrocytes against induced hemoglobin oxidation, lipid peroxidation, and hemolysis. The phytochemical profile of the Abrantes’ bee pollen revealed twenty phenolic compounds, identified by high-performance liquid chromatography with electrospray ionization mass spectrometry coupled with photodiode array detection. Among them, quercetin derivatives were the most abundant. Concerning the biological potential, the pollen extract showed notable capacity for 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, and superoxide radicals, as well as for inhibition of α-glucosidase action, and protection of human erythrocytes against oxidative damage. Non-cytotoxic effects regarding the NHDF normal cell line, human adenocarcinoma Caco-2, and human liver HepG2 cells were observed. The results obtained contributed to further research on modes of action related to oxidative damage and metabolic health problems, to generate deeper knowledge of potential health-promoting effects to develop novel pharmaceutical drugs, nutraceuticals, and dietary supplements.

scholarly journals Dramatic Increase in Content of Diverse Flavonoids Accompanied with Down-Regulation of F-Box Genes in a Chrysanthemum (Chrysanthemum × morifolium (Ramat.) Hemsl.) Mutant Cultivar Producing Dark-Purple Ray Florets

Genes ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 865
Author(s):  
Yeong Deuk Jo ◽  
Jaihyunk Ryu ◽  
Ye-Sol Kim ◽  
Kyung-Yun Kang ◽  
Min Jeong Hong ◽  
...  
Keyword(s):  
Translational Regulation ◽  
High Performance ◽  
Flower Color ◽  
Chrysanthemum Morifolium ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Genes Encoding ◽  
Ray Florets ◽  
Chrysanthemum Morifolium Ramat ◽  
Flavonoid Accumulation

Anthocyanins (a subclass of flavonoids) and flavonoids are crucial determinants of flower color and substances of pharmacological efficacy, respectively, in chrysanthemum. However, metabolic and transcriptomic profiling regarding flavonoid accumulation has not been performed simultaneously, thus the understanding of mechanisms gained has been limited. We performed HPLC-DAD-ESI-MS (high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization mass spectrometry) and transcriptome analyses using “ARTI-Dark Chocolate” (AD), which is a chrysanthemum mutant cultivar producing dark-purple ray florets, and the parental cultivar “Noble Wine” for metabolic characterization and elucidation of the genetic mechanism determining flavonoid content. Among 26 phenolic compounds identified, three cyanidins and eight other flavonoids were detected only in AD. The total amounts of diverse flavonoids were 8.0 to 10.3 times higher in AD. Transcriptome analysis showed that genes in the flavonoid biosynthetic pathway were not up-regulated in AD at the early flower stage, implying that the transcriptional regulation of the pathway did not cause flavonoid accumulation. However, genes encoding post-translational regulation-related proteins, especially F-box genes in the mutated gene, were enriched among down-regulated genes in AD. From the combination of metabolic and transcriptomic data, we suggest that the suppression of post-translational regulation is a possible mechanism for flavonoid accumulation in AD. These results will contribute to research on the regulation and manipulation of flavonoid biosynthesis in chrysanthemum.

scholarly journals Is Our Natural Food Our Homeostasis? Array of a Thousand Effect-Directed Profiles of 68 Herbs and Spices

2021 ◽  
Vol 12 ◽  
Author(s):  
Tamara Schreiner ◽  
Dorena Sauter ◽  
Maren Friz ◽  
Julia Heil ◽  
Gertrud Elisabeth Morlock
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Reversed Phase ◽  
Natural Food ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Bioactive Substances ◽  
Traditional Medicines ◽  
Beneficial Effects ◽  
Herbs And Spices

The beneficial effects of plant-rich diets and traditional medicines are increasingly recognized in the treatment of civilization diseases due to the abundance and diversity of bioactive substances therein. However, the important active portion of natural food or plant-based medicine is presently not under control. Hence, a paradigm shift from quality control based on marker compounds to effect-directed profiling is postulated. We investigated 68 powdered plant extracts (botanicals) which are added to food products in food industry. Among them are many plants that are used as traditional medicines, herbs and spices. A generic strategy was developed to evaluate the bioactivity profile of each botanical as completely as possible and to straightforwardly assign the most potent bioactive compounds. It is an 8-dimensional hyphenation of normal-phase high-performance thin-layer chromatography with multi-imaging by ultraviolet, visible and fluorescence light detection as well as effect-directed assay and heart-cut of the bioactive zone to orthogonal reversed-phase high-performance liquid chromato-graphy−photodiode array detection−heated electrospray ionization mass spectrometry. In the non-target, effect-directed screening via 16 different on-surface assays, we tentatively assigned more than 60 important bioactive compounds in the studied botanicals. These were antibacterials, estrogens, antiestrogens, androgens, and antiandrogens, as well as acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, β-glucosidase, β-glucuronidase, and tyrosinase inhibitors, which were on-surface heart-cut eluted from the bioautogram or enzyme inhibition autogram to the next dimension for further targeted characterization. This biological-physicochemical hyphenation is able to detect and control active mechanisms of traditional medicines or botanicals as well as the essentials of plant-based food. The array of 1,292 profiles (68 samples × 19 detections) showed the versatile bioactivity potential of natural food. It reveals how efficiently and powerful our natural food contributes to our homeostasis.

scholarly journals Effects of Pigment Constituents and Their Distribution on Spathe Coloration of Zantedeschia hybrida

HortScience ◽  
2017 ◽  
Vol 52 (12) ◽  
pp. 1840-1848
Author(s):  
Ting Lei ◽  
Yang Song ◽  
Xuehua Jin ◽  
Tianyu Su ◽  
Yiwen Pu
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Performance ◽  
Colorimetric Method ◽  
Ultra Performance Liquid Chromatography ◽  
Color Variation ◽  
Ionization Mass ◽  
Photodiode Array Detection ◽  
Total Anthocyanin ◽  
Different Color

The plant Zantedeschia hybrida is colorful and suitable for cut flowers and potted plants. This study employed a colorimetric method for the determination of spathe color phenotypes in 27 Z. hybrida cultivars and classified them into six major color classes. To characterize the coloration mechanism of the Z. hybrida spathe, this study explored the main colorants and pigment distribution using high-performance liquid chromatography (HPLC) with photodiode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS), ultra-performance liquid chromatography/hybrid triple quadrupole linear ion trap mass spectrometry (UPLC-Q-TRAP-MS), and tissue sections. The results showed that flavonoids were colorants in the spathes of different color groups and that cyanidin (Cy) was the main colorant, whereas carotenoids were not detected in the spathe. Total anthocyanin (TA) content was negatively correlated with lightness (L*) of coloration, such that a spathe with a higher TA and thicker pigmented cell layer showed a deeper color; however, there was no correlation between deep coloration in a spathe and flattened upper epidermal cells. The difference in TA was the main reason for the color variation among Z. hybrida of different color groups, whereas the total flavones and flavonols (TF) played a key role in the coloration of the orange and yellow group.

scholarly journals LC/PDA/ESI-MS Profiling and Radical Scavenging Activity of Anthocyanins in Various Berries

2004 ◽  
Vol 2004 (5) ◽  
pp. 241-247 ◽  
Author(s):  
Jun-ichiro Nakajima ◽  
Ippei Tanaka ◽  
Shujiro Seo ◽  
Mami Yamazaki ◽  
Kazuki Saito
Keyword(s):  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Distribution Patterns ◽  
Ionization Mass ◽  
Black Currant ◽  
Photodiode Array Detection ◽  
Vaccinium Ashei ◽  
Esi Ms ◽  
Berry Extracts

Anthocyanin extracts of two blueberries,Vaccinium myrtillus(bilberry) andVaccinium ashei(rabbiteye blueberry), and of three other berries,Ribes nigrum(black currant),Aronia melanocarpa(chokeberry), andSambucus nigra(elderberry), were analyzed by high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization - mass spectrometry (LC/PDA/ESI-MS). Both bilberry and rabbiteye blueberry contained 15 identical anthocyanins with different distribution patterns. Black currant, chokeberry, and elderberry contained 6, 4, and 4 kinds of anthocyanins, respectively. The radical scavenging activities of these berry extracts were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH). All these extracts showed potent antiradical activities.

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