scholarly journals Tissue Adhesion-Anisotropic Polyrotaxane Hydrogels Bilayered with Collagen

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 168
Author(s):  
Masahiro Hakariya ◽  
Yoshinori Arisaka ◽  
Hiroki Masuda ◽  
Tetsuya Yoda ◽  
Atsushi Tamura ◽  
...  
Keyword(s):  
Rational Design ◽  
Cellular Responses ◽  
Biological Tissues ◽  
Target Tissue ◽  
Tissue Engineering Scaffolds ◽  
Collagen Hydrogel ◽  
Cyclic Molecules ◽  
Strong Adhesion ◽  
Ft Ir ◽  
Collagen Layer

Hydrogels are promising materials in tissue engineering scaffolds for healing and regenerating damaged biological tissues. Previously, we developed supramolecular hydrogels using polyrotaxane (PRX), consisting of multiple cyclic molecules threaded by an axis polymer for modulating cellular responses. However, since hydrogels generally have a large amount of water, their adhesion to tissues is extremely weak. Herein, we designed a bilayered hydrogel with a PRX layer and a collagen layer (PRX/collagen hydrogel) to achieve rapid and strong adhesion to the target tissue. The PRX/collagen hydrogel was fabricated by polymerizing PRX crosslinkers in water with placement of a collagen sponge. The differences in components between the PRX and collagen layers were analyzed using Fourier transform infrared spectroscopy (FT-IR). After confirming that the fibroblasts adhered to both layers of the PRX/collagen hydrogels, the hydrogels were implanted subcutaneously in mice. The PRX hydrogel without collagen moved out of its placement site 24 h after implantation, whereas the bilayer hydrogel was perfectly adherent at the site. Together, these findings indicate that the bilayer structure generated using PRX and collagen may be a rational design for performing anisotropic adhesion.

Reconstituted Extracellular Matrix Improve Osteoblastic Differentiation onto Titanium Surfaces

2012 ◽  
Vol 706-709 ◽  
pp. 584-588
Author(s):  
Lia Rimondini ◽  
Federica Demarosi ◽  
Ismaela Foltran ◽  
Nadia Quirici
Keyword(s):  
Extracellular Matrix ◽  
Electrostatic Force ◽  
Biological Tissues ◽  
Osteoblastic Differentiation ◽  
Cell Phenotype ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
Differentiation Potential ◽  
Oral Implants

Electrospinning technique is an efficient processing method to manufacture micro-and nanosized fibrous structures by electrostatic force for different applications. In biomaterial field, electrospinning technique has been successfully utilized to prepare new drug delivery materials and tissue engineering scaffolds. Fiber mats of biodegradable polymers having a diameter in the nanoto submicro-scale can be considered to mimic the nanofibrous structure of native extracellular matrix (ECM). Native extracellular matrix, constituted of proteins and polysaccharides improving cells growth in its nanofibrous porous structure, controls not only the cell phenotype, but the whole structure of the biological tissues. In the present study we investigated the effect of electrospun reconstituted collagen fibers onto metals for oral implants devices manufacturing as far as the osteoblastic differentiation potential of stem cells and cytofunctionality of osteoblasts in-vitro. The cells cultured onto titanium samples coated with ECM constituents showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates.

scholarly journals Methotrexate binding causes structural and functional changes in lung cystatin.

2010 ◽  
Vol 57 (4) ◽  
Author(s):  
Mohd Shahnawaz Khan ◽  
Medha Priyadarshini ◽  
Sadia Sumbul ◽  
Bilqees Bano
Keyword(s):  
Structural Integrity ◽  
Hydrophobic Interactions ◽  
Tissue Injury ◽  
Cysteine Protease Inhibitor ◽  
Target Tissue ◽  
Protein Fluorescence ◽  
Binding Interaction ◽  
Functional Changes ◽  
Ft Ir ◽  
Goat Lung Cystatin

Regulation of cysteine proteinases and their inhibitors is of utmost importance in diseases like lung cancer, chronic inflammatory conditions such as asthma, emphysema, and idiopathic pulmonary fibrosis. Protease-antiprotease imbalance accelerates disease progression. In the present study, the effect of antineoplastic and antirheumatic drug methotrexate (MTX) on lung cystatin (a cysteine protease inhibitor) was studied to explore drug induced changes in functional and structural integrity of the protein. The basic binding interaction was studied by UV-absorption, FT-IR and fluorescence spectroscopy. The quenching of protein fluorescence confirmed the binding of MTX with goat lung cystatin (GLC-I). Stern-Volmer analysis of MTX-GLC-I system at different temperatures indicates the presence of static component in the quenching mechanism. The thermodynamic parameters ΔH⁰ and ΔS⁰ were -3.8 kJ/mol and 94.97 J•mol⁻¹•K⁻¹, respectively, indicating that both hydrogen bonds and hydrophobic interactions played a major role in the binding of MTX to GLC-I. Methotrexate (7 µM) caused complete inactivation of lung cystatin after 6 hours. The results of FT-IR spectroscopy reflect perturbation of the goat lung cystatin on interaction with MTX. Methotrexate induced loss of function change in the inhibitor could provide a rationale for the off target tissue injury caused by the drug and for the design of agents against such an injury.

scholarly journals Spectrochemical Micro-Analysis of Biological Tissues Using Raman and FT-IR Microspectroscopy with Metalographic Polishing

2005 ◽  
Vol 11 (S02) ◽  
Author(s):  
V L St. Jeor ◽  
D L Elmore ◽  
C A Lendon ◽  
S A Smith ◽  
C L Leverette
Keyword(s):  
Biological Tissues ◽  
Ft Ir ◽  
Ir Microspectroscopy ◽  
Micro Analysis

scholarly journals Macromolecular Orientation in Biological Tissues Using a Four-Polarization Method in FT-IR Imaging

2020 ◽  
Vol 92 (19) ◽  
pp. 13313-13318
Author(s):  
Paulina Koziol ◽  
Danuta Liberda ◽  
Wojciech M. Kwiatek ◽  
Tomasz P. Wrobel
Keyword(s):  
Biological Tissues ◽  
Polarization Method ◽  
Ir Imaging ◽  
Ft Ir

scholarly journals Spatially resolved macromolecular orientation in biological tissues using FT-IR imaging

2021 ◽  
pp. 100013
Author(s):  
Karolina Kosowska ◽  
Paulina Koziol ◽  
Danuta Liberda ◽  
Tomasz P. Wrobel
Keyword(s):  
Biological Tissues ◽  
Spatially Resolved ◽  
Ir Imaging ◽  
Ft Ir

Compositionally Modified Hydroxyapatite Nanocrystals for Polymer/Ceramic Scaffold Applications

2005 ◽  
Vol 897 ◽  
Author(s):  
Andrei Stanishevsky ◽  
Peserai Chinoda ◽  
Shafiul Chowdhury ◽  
Vinoy Thomas ◽  
Aaron Catledge ◽  
...  
Keyword(s):  
Polymeric Materials ◽  
Chemical Precipitation ◽  
X Ray Diffraction ◽  
Tissue Engineering Scaffolds ◽  
Force Microscopy ◽  
Ceramic Scaffold ◽  
Atomic Force ◽  
Ft Ir ◽  
Hydroxyapatite Nanocrystals ◽  
Bioresorbable Implants

AbstractThe polymer/bioceramic composite materials attract much attention for the development of bioresorbable implants and tissue engineering scaffolds. Hydroxyapatite (HA) is the most commonly used bioceramic material due to its similarity to the major mineral component of the hard tissue. We synthesized carbonated and Mg-substituted HA nanocrystals with various concentrations of CO32− and Mg2+ ions by chemical precipitation in the range of the process temperatures from 25 °C to 100 °C.The HA nanocrystals were mixed with several polymeric materials (PCL, PLA, PVA, collagen) to fabricate bulk and nanofiber polymer/HA nanoparticle composites with the HA loading up to 80 % by weight. The HA nanocrystals and polymer/HA composites were characterized by X-ray diffraction, FT-IR spectroscopy, scanning electron and atomic force microscopy. Mechanical properties of the composites were investigated using nanoindentation technique.

scholarly journals Design of RGDS Peptide-Immobilized Self-Assembling β-Strand Peptide from Barnacle Protein

2021 ◽  
Vol 22 (3) ◽  
pp. 1240
Author(s):  
Daisuke Fujii ◽  
Kento Takase ◽  
Ami Takagi ◽  
Kei Kamino ◽  
Yoshiaki Hirano
Keyword(s):  
Cell Adhesion ◽  
Cell Attachment ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Tissue Engineering Scaffolds ◽  
Self Assembling ◽  
Polystyrene Plate ◽  
Coated Cell ◽  
Ft Ir ◽  
Adhesive Proteins

We designed three types of RGD-containing barnacle adhesive proteins using self-assembling peptides. In the present study, three types of RGD-containing peptides were synthesized by solid-phase peptide synthesis, and the secondary structures of these peptides were analyzed by CD and FT-IR spectroscopy. The mechanical properties of peptide hydrogels were characterized by a rheometer. We discuss the correlation between the peptide conformation, and cell attachment and cell spreading activity from the viewpoint of developing effective tissue engineering scaffolds. We created a peptide-coated cell culture substrate by coating peptides on a polystyrene plate. They significantly facilitated cell adhesion and spreading compared to a non-coated substrate. When the RGDS sequence was modified at N- or C-terminal of R-Y, it was found that the self-assembling ability was dependent on the strongly affects hydrogel formation and cell adhesion caused by its secondary structure.

scholarly journals Small Ruminant Models for Articular Cartilage Regeneration by Scaffold-Based Tissue Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Liqing Peng ◽  
Bin Zhang ◽  
Xujiang Luo ◽  
Bo Huang ◽  
Jian Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Animal Models ◽  
Rational Design ◽  
Small Ruminant ◽  
Cartilage Regeneration ◽  
Large Animal ◽  
Tissue Engineering Scaffolds ◽  
Therapeutic Concepts ◽  
Articular Cartilage Regeneration

Animal models play an important role in preclinical studies, especially in tissue engineering scaffolds for cartilage repair, which require large animal models to verify the safety and effectiveness for clinical use. The small ruminant models are most widely used in this field than other large animals because they are cost-effective, easy to raise, not to mention the fact that the aforementioned animal presents similar anatomical features to that of humans. This review discusses the experimental study of tissue engineering scaffolds for knee articular cartilage regeneration in small ruminant models. Firstly, the selection of these scaffold materials and the preparation process in vitro that have been already used in vivo are briefly reviewed. Moreover, the major factors influencing the rational design and the implementation as well as advantages and limitations of small ruminants are also demonstrated. As regards methodology, this paper applies principles and methods followed by most researchers in the process of experimental design and operation of this kind. By summarizing and comparing different therapeutic concepts, this paper offers suggestions aiming to increase the effectiveness of preclinical research using small ruminant models and improve the process of developing corresponding therapies.

scholarly journals Tunable Adhesion for Bio-Integrated Devices

Micromachines ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 529 ◽  
Author(s):  
Zhaozheng Yu ◽  
Huanyu Cheng
Keyword(s):  
Rapid Development ◽  
Chemical Properties ◽  
Biological Tissues ◽  
Tissue Adhesives ◽  
Adhesion Properties ◽  
Effective Strategies ◽  
Integrated Devices ◽  
Strong Adhesion ◽  
Novel Material ◽  
Physical And Chemical

With the rapid development of bio-integrated devices and tissue adhesives, tunable adhesion to soft biological tissues started gaining momentum. Strong adhesion is desirable when used to efficiently transfer vital signals or as wound dressing and tissue repair, whereas weak adhesion is needed for easy removal, and it is also the essential step for enabling repeatable use. Both the physical and chemical properties (e.g., moisture level, surface roughness, compliance, and surface chemistry) vary drastically from the skin to internal organ surfaces. Therefore, it is important to strategically design the adhesive for specific applications. Inspired largely by the remarkable adhesion properties found in several animal species, effective strategies such as structural design and novel material synthesis were explored to yield adhesives to match or even outperform their natural counterparts. In this mini-review, we provide a brief overview of the recent development of tunable adhesives, with a focus on their applications toward bio-integrated devices and tissue adhesives.

Visualization of Heat Shock Proteins for Quantifying Laser-Induced Thermal Ablation of Biological Tissues

2011 ◽  
Author(s):  
Amir Y. Sajjadi ◽  
Kunal Mitra ◽  
Michael S. Grace
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Laser Irradiation ◽  
Expression Patterns ◽  
Biological Tissues ◽  
Threshold Temperature ◽  
Target Tissue ◽  
Laser Induced Damage ◽  
Shock Proteins ◽  
Over Time

In laser-based therapeutics, it is important to ablate target tissue with minimal damage to surrounding healthy tissue. Unique properties of lasers allow precise and controlled ablation of tissue. Tightly focusing a short-pulse laser at the desired tissue region and controlling the exposure time by scanning the beam at the target can minimize corresponding collateral damage [1]. Even so, design of effective laser-based ablation procedures requires an understanding of the extent of laser-induced damage for given laser parameters (power, intensity, duration, etc.). Therefore, the instantaneous and effects over time of laser irradiation in live tissue should be studied. Instantaneous effects can be quantified by measuring thermal effects of laser irradiation on tissue. Depending on the application, threshold temperature is necessary to make permanent or temporary changes in tissue structure [1]. The temperature profile around the laser-irradiated region gives insight into radial energy spread and the extent of damage in tissue surrounding the ablation zone. In order to investigate the effects over time of laser irradiation of tissue, we studied the temporal expression patterns heat shock proteins (HSP), members of a class of proteins whose expression patterns change when cells are exposed to elevated temperature or other stressors [2]. We conducted experiments on live anesthetized mice to determine the spatiotemporal expression patterns of heat shock proteins in skin tissue after laser stimulation, both to understand the roles of heat shock proteins in laser-induced tissue damage and repair, and to develop heat shock proteins as tools to illustrate the extent of laser-induced damage and wound healing following irradiation.

Sign in / Sign up

Export Citation Format

Share Document