scholarly journals Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae

Genes ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 624 ◽  
Author(s):  
Annabella Königer ◽  
Sonja Grath
Keyword(s):  
Candidate Genes ◽  
Cold Tolerance ◽  
Cold Hardiness ◽  
Cold Shock ◽  
Drosophila Ananassae ◽  
Tropical Species ◽  
Seasonal Temperature ◽  
Tropical Regions ◽  
Chill Coma ◽  
Before And After

Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regions. Phenotypic assays of chill coma recovery time (CCRT) together with previously published population genetic data suggest that only a small number of genes underlie improved cold hardiness in the cold-adapted populations. We used high-throughput RNA sequencing to analyze differential gene expression before and after exposure to a cold shock in coldtolerant lines (those with fast chill coma recovery, CCR) and cold-sensitive lines (slow CCR) from a population originating from Bangkok, Thailand (the ancestral species range). We identified two candidate genes with a significant interaction between cold tolerance and cold shock treatment: GF14647 and GF15058. Further, our data suggest that selection for increased cold tolerance did not operate through the increased activity of heat shock proteins, but more likely through the stabilization of the actin cytoskeleton and a delayed onset of apoptosis.

scholarly journals Three quantitative trait loci explain more than 60% of phenotypic variation for chill coma recovery time in Drosophila ananassae

2019 ◽  
Author(s):  
Annabella Königer ◽  
Saad Arif ◽  
Sonja Grath
Keyword(s):  
Candidate Genes ◽  
Cold Tolerance ◽  
Quantitative Trait ◽  
Recovery Time ◽  
Differentially Expressed ◽  
Drosophila Ananassae ◽  
Phenotypic Variance ◽  
Climatic Fluctuations ◽  
Standard Interval ◽  
Chill Coma

AbstractEctothermic species such as insects are particularly vulnerable to climatic fluctuations. Nevertheless, many insects that evolved and diversified in the tropics have successfully colonized temperate regions all over the globe. To shed light on the genetic basis of cold tolerance in such species, we conducted a quantitative trait locus (QTL) mapping experiment for chill coma recovery time (CCRT) in Drosophila ananassae, a cosmopolitan species that has expanded its range from tropical to temperate regions.We created a mapping population of recombinant inbred advanced intercross lines (RIAILs) from two founder strains with diverging CCRT phenotypes. The RIAILs were phenotyped for their CCRT and, together with the founder strains, genotyped for polymorphic markers with double-digest restriction site-associated DNA (ddRAD) sequencing. Using a hierarchical mapping approach that combined standard interval mapping and a multiple-QTL model, we mapped three QTL which altogether explained 64% of the phenotypic variance. For two of the identified QTL, we found evidence of epistasis. To narrow down the list of cold tolerance candidate genes, we cross-referenced the QTL intervals with genes that we previously identified as differentially expressed in response to cold in D. ananassae, and with thermotolerance candidate genes of D. melanogaster. Among the 58 differentially expressed genes that were contained within the QTL, GF15058 showed a significant interaction of the CCRT phenotype and gene expression. Further, we identified the orthologs of four D. melanogaster thermotolerance candidate genes, MtnA, klarsicht, CG5246 (D.ana/GF17132) and CG10383 (D.ana/GF14829) as candidates for cold tolerance in D. ananassae.

Cold Tolerance of the Tribolium castaneum (Coleoptera: Tenebrionidae), Under Different Thermal Regimes: Impact of Cold Acclimation

2019 ◽  
Vol 112 (4) ◽  
pp. 1983-1988 ◽  
Author(s):  
H Izadi ◽  
M Mohammadzadeh ◽  
M Mehrabian
Keyword(s):  
Cold Acclimation ◽  
Cold Tolerance ◽  
Tribolium Castaneum ◽  
Cold Hardiness ◽  
Red Flour Beetle ◽  
Flour Beetle ◽  
Chill Coma ◽  
Rapid Cold Hardening ◽  
Thermal Regimes ◽  
Coleoptera Tenebrionidae

Abstract The red flour beetle, Tribolium castaneum (Herbst), is a serious pest of stored product worldwide. Cold tolerance or cold hardiness is an important ecophysiological trait related directly to survival, fitness, and distribution of insects. In this study, the effects of four thermal regimes, i.e., control (C), cold acclimation (CA), rapid cold hardening (RCH), and fluctuating-acclimation (FA), were examined for their effects on cold tolerance, supercooling point (SCP), lower lethal temperature (LLT), and chill-coma recovery time (CCRT) of the red flour beetle. In addition, changes in cryoprotectant (trehalose, sorbitol, and myo-inositol) levels were investigated under each thermal treatment. The results documented a substantial enhancement in the SCP, cold hardiness, and cryoprotectant levels of the adults of T. castaneum under CA regimes. The lowest SCP, highest trehalose and myo-inositol contents, and, subsequently, the greatest survival rate were observed in cold-acclimated beetles. In addition, coordination between cryoprotectant level, SCP, and cold tolerance of the pest was observed. The highest and lowest CCRT were observed at control and CA, respectively. In RCH regime with the highest impact, LLT reached the lowest level of −22°C. As most of the mortality of T. castaneum occurred at a temperature above the SCP, so this pest could be considered as a chill-susceptible insect.

scholarly journals Genome-Wide Association Study Reveals the Genetic Basis of Cold Tolerance in Rice at the Seedling Stage

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 318
Author(s):  
Tae-Ho Ham ◽  
Yebin Kwon ◽  
Yoonjung Lee ◽  
Jisu Choi ◽  
Joohyun Lee
Keyword(s):  
Association Study ◽  
Candidate Genes ◽  
Cold Tolerance ◽  
Genome Wide Association Study ◽  
Seedling Stage ◽  
Genome Wide Association ◽  
Matrix Analysis ◽  
Rice Seedlings ◽  
Genome Wide ◽  
A Genome

We conducted a genome-wide association study (GWAS) of cold tolerance in a collection of 127 rice accessions, including 57 Korean landraces at the seedling stage. Cold tolerance of rice seedlings was evaluated in a growth chamber under controlled conditions and scored on a 0–9 scale, based on their low-temperature response and subsequent recovery. GWAS, together with principal component analysis (PCA) and kinship matrix analysis, revealed four quantitative trait loci (QTLs) on chromosomes 1, 4, and 5 that explained 16.5% to 18.5% of the variance in cold tolerance. The genomic region underlying the QTL on chromosome four overlapped with a previously reported QTL associated with cold tolerance in rice seedlings. Similarly, one of the QTLs identified on chromosome five overlapped with a previously reported QTL associated with seedling vigor. Subsequent bioinformatic and haplotype analyses revealed three candidate genes affecting cold tolerance within the linkage disequilibrium (LD) block of these QTLs: Os01g0357800, encoding a pentatricopeptide repeat (PPR) domain-containing protein; Os05g0171300, encoding a plastidial ADP-glucose transporter; and Os05g0400200, encoding a retrotransposon protein, Ty1-copia subclass. The detected QTLs and further evaluation of these candidate genes in the future will provide strategies for developing cold-tolerant rice in breeding programs.

Genetic Recombination in Coprinus

1970 ◽  
Vol 6 (3) ◽  
pp. 669-678
Author(s):  
B. C. LU
Keyword(s):  
Fruiting Body ◽  
Temperature Effects ◽  
Cold Shock ◽  
Genetic Recombination ◽  
Recombination Frequency ◽  
Temperature Treatment ◽  
Sensitive Period ◽  
Crossing Over ◽  
Synaptinemal Complex ◽  
Before And After

The frequency of genetic recombination in Coprinus lagopus may be modified by heat and cold shock. By removal of samples from a fruiting body before and after temperature treatment, it is possible to study the ultrastructure of chromosomes at the time recombination frequency (between den+ x +me-1) can be modified. The sensitive period for temperature effects and, therefore, probably the time of crossing over, commences with the formation of the synaptinemal complex (S.C.) and ends with its disappearance, i.e. during the entire existence of the S.C. It is concluded that recombination is an event subsequent to the formation of the S.C. and is independent of the process of its formation. It is suggested that the event takes place at the synaptic centre.

scholarly journals Greenhouses – urban biodiversity hotspot of alien Oribatida (Acari) species

2020 ◽  
Vol 63 (1) ◽  
pp. 1-10
Author(s):  
Mateusz OSZUST ◽  
◽  
Ziemowit OLSZANOWSKI ◽  
Marta PRZYMUSZAŁA ◽  
d Aleksandra JAGIEŁŁO ◽  
...  
Keyword(s):  
Alien Species ◽  
Native Species ◽  
Biodiversity Hotspot ◽  
Oribatid Mites ◽  
Future Research ◽  
Tropical Species ◽  
Tropical Regions ◽  
Stable Conditions ◽  
The World ◽  
Different Parts

Palm houses and other greenhouses, due to maintaining constant temperature and humidity, allow the cultivation in Europe of plants from different parts of the world, even from tropical regions. However, sometimes they are also a habitat for alien species of spiders, mites, insect, etc. These animals have been introduced accidentally with contaminated plants, seeds, seedlings, soil and thanks to stable conditions maintained in greenhouses, they may colonize these places. Example of arthropods, of which even tropical species occur in several greenhouses, are oribatid mites – minute saprophagous arachnids that mostly inhabit soil. In Europe they are represented by about 2,000 species, while worldwide – over 10,000 taxa were described. The aim of this research was to investigate the selected greenhouses for the biodiversity of oribatid mites and the presence of non-native species. In total, 49 taxa were recorded, including 23 alien species (for example, a Neotropical taxon Galumna hamifer, or Oriental Suctobelbella parallelodentata). These results confirm that greenhouses are the places of occurrence of many alien oribatid species. The obtained results may be used in future research on the biology of poorly known tropical mites.

scholarly journals The impact of seasonality on niche breadth, distribution range and species richness: a theoretical exploration of Janzen's hypothesis

2016 ◽  
Vol 283 (1835) ◽  
pp. 20160349 ◽  
Author(s):  
Xia Hua
Keyword(s):  
Temperature Variation ◽  
Empirical Studies ◽  
Tropical Species ◽  
Seasonal Temperature ◽  
Thermal Niche ◽  
Trade Offs ◽  
Seasonal Temperature Variation ◽  
Latitudinal Patterns ◽  
The Tropics ◽  
The Impact

Being invoked as one of the candidate mechanisms for the latitudinal patterns in biodiversity, Janzen's hypothesis states that the limited seasonal temperature variation in the tropics generates greater temperature stratification across elevations, which makes tropical species adapted to narrower ranges of temperatures and have lower effective dispersal across elevations than species in temperate regions. Numerous empirical studies have documented latitudinal patterns in species elevational ranges and thermal niche breadths that are consistent with the hypothesis, but the theoretical underpinnings remain unclear. This study presents the first mathematical model to examine the evolutionary processes that could back up Janzen's hypothesis and assess the effectiveness of limited seasonal temperature variation to promote speciation along elevation in the tropics. Results suggest that trade-offs in thermal tolerances provide a mechanism for Janzen's hypothesis. Limited seasonal temperature variation promotes gradient speciation not due to the reduction in gene flow that is associated with narrow thermal niche, but due to the pleiotropic effects of more stable divergent selection of thermal tolerance on the evolution of reproductive incompatibility. The proposed modelling approach also provides a potential way to test a speciation model against genetic data.

The European charcoal trade

IAWA Journal ◽  
2020 ◽  
Vol 41 (4) ◽  
pp. 463-477 ◽  
Author(s):  
Volker Haag ◽  
Valentina Theresia Zemke ◽  
Tim Lewandrowski ◽  
Johannes Zahnen ◽  
Peter Hirschberger ◽  
...  
Keyword(s):  
Flow Analysis ◽  
Trade Flow ◽  
Tropical Species ◽  
Tropical Regions ◽  
Eu Member States ◽  
Microscopy Technique ◽  
Proportional Relation ◽  
Reflected Light ◽  
Timber Market ◽  
Careful Check

Abstract About half the wood extracted worldwide from forests is used as fuelwood to produce energy, about 17 percent is converted to charcoal (FAO 2017) which represents one of the least controlled/monitored segments of the European timber market. Although charcoal has a significant share on the European market of wood-based products it is not yet covered by the European Timber Regulations (EUTR), (EU) No 995/2010. For this project, a total of 150 charcoal consignments from eleven countries (Germany, Poland, Switzerland, Spain, Italy, Norway, Denmark, Netherlands, Ukraine, Czech Republic, and Belgium) were examined and evaluated based on the 3D-reflected light microscopy technique. The high-resolution study indicates the proportion of different European timbers compared with that of timbers from subtropical and tropical regions. The share of subtropical and tropical species is surprisingly high with approximately 46% for material received from all countries studied, but far over 60% for Spain, Italy, Poland, and Belgium. The study shows that comparing the results for charcoal received from these countries there is an inversely proportional relation of certified products (FSC and PEFC) and products with timbers from subtropical or tropical origins. In the charcoal consignments from Switzerland, the share of timbers from subtropical or tropical origin is only 13.5%, whereas that of certified products is 60%. In material received from Spain, the proportion of timbers from subtropical or tropical regions is 67%, whereas that of certified products only 8%. A careful check of the declaration on the packaging, of the accompanying certificates, and the information on origin revealed alarming evidence: only 25% of the consignments examined provide information on the bags, e.g., with regard to the processed wood species; and well over half of such declarations were incorrect and/or incomplete. A trade flow analysis of EU member states was carried out to contribute to a better understanding of the relationships between international charcoal trade flows and the end products in European countries. This approach contributes to an essential understanding of charcoal transit in Europe and the results constitute a strong motive for the inclusion of charcoal in the respective annex to the EUTR.

scholarly journals Surviving the Antarctic Winter—Life Stage Cold Tolerance and Ice Entrapment Survival in The Invasive Chironomid Midge Eretmoptera murphyi

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 147 ◽  
Author(s):  
Jesamine C. Bartlett ◽  
Peter Convey ◽  
Scott A. L. Hayward
Keyword(s):  
Cold Tolerance ◽  
Cold Hardiness ◽  
Life Stage ◽  
Western Coast ◽  
Critical Determinant ◽  
Signy Island ◽  
Detailed Assessment ◽  
Freeze Tolerant ◽  
The Antarctic ◽  
Assessment Of Stress

An insect’s ability to tolerate winter conditions is a critical determinant of its success. This is true for both native and invasive species, and especially so in harsh polar environments. The midge Eretmoptera murphyi (Diptera, Chironomidae) is invasive to maritime Antarctic Signy Island, and the ability of fourth instar larvae to tolerate freezing is hypothesized to allow the species to extend its range further south. However, no detailed assessment of stress tolerance in any other life stage has yet been conducted. Here, we report that, although larvae, pupae and adults all have supercooling points (SCPs) of around −5 °C, only the larvae are freeze-tolerant, and that cold-hardiness increases with larval maturity. Eggs are freeze-avoiding and have an SCP of around −17 °C. At −3.34 °C, the CTmin activity thresholds of adults are close to their SCP of −5 °C, and they are likely chill-susceptible. Larvae could not withstand the anoxic conditions of ice entrapment or submergence in water beyond 28 d. The data obtained here indicate that the cold-tolerance characteristics of this invasive midge would permit it to colonize areas further south, including much of the western coast of the Antarctic Peninsula.

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