scholarly journals A New Therapy for Uncomplicated Vulvovaginal Candidiasis and Its Impact on Vaginal Flora

Healthcare ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1555
Author(s):  
Guglielmo Stabile ◽  
Roberta Marie Gentile ◽  
Stefania Carlucci ◽  
Stefano Restaino ◽  
Francesco De Seta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
New Product ◽  
Vaginal Flora ◽  
Vulvovaginal Candidiasis ◽  
Azole Resistance ◽  
Wet Mount ◽  
High Incidence ◽  
Microbiological Cure ◽  
New Strategies ◽  
Better Than

Background: An estimated 75% of women will have one episode of vulvovaginal candidiasis (VCC) during their lifetime, and 40–50% of these will experience further episodes. The high incidence of vulvovaginal candidiasis, combined with the problems of azole resistance and toxicity, highlights the necessity for new strategies for the treatment of this condition. In this context, natural compounds represent promising alternatives. Methods: We enrolled, between January 2020 and April 2021, forty women affected by uncomplicated vulvovaginal candidiasis. Women were divided into two groups. In the first group, we treated 20 women with clotrimazole daily administration for six days. In the second group, 20 women were treated with clotrimazole associated with Unilen® Microbio+, a new product containing Saccharomyces cerevisiae, melatonin, and GLA-14. Women underwent a check at days 15, 30, and 90. A clinical and cultural examination were performed to establish the effect of the treatments on vaginal flora. Results: In the group treated with Unilen® Microbio+, clinical and microbiological cure at 15 and 30 days was observed in 18 women (90%), compared with 16 women (80%) in the group treated only with clotrimazole. The efficacy of the association between clotrimazole and Unilen® Microbio+ in these uncomplicated forms was therefore not inferior to the azole treatment alone. Only four women (20%) in the Unilen® Microbio+ group presented symptomatic recurrences within the 3 months, compared with eight women (40%) in the clotrimazole-only group. Microscopic wet mount analysis at 1 and 3 months demonstrated a significant increase in lactobacillus count and a reduction in the polymorphonucleate cells in the Unilen® Microbio+ group. Conclusions: Unilen® Microbio+ supplementation was demonstrated to cure uncomplicated vulvovaginal candidiasis with clotrimazole, reducing recurrence and improving vaginal flora better than clotrimazole treatment alone.

Comparative Study Between Clean Intermittent Self-Catheterization (Cisc) and Continuous Indwelling Catheterization (Cidc) in Relieving Acute Refractory Retention of Urine Due to Benign Enlargement of Prostate (Bep)

2020 ◽  
Vol 21 (2) ◽  
pp. 105-110
Author(s):  
Md Shawkat Alam ◽  
Sudip Das Gupta ◽  
Hadi Zia Uddin Ahmed ◽  
Md Saruar Alam ◽  
Sharif Muhammod Wasimuddin
Keyword(s):  
Comparative Study ◽  
Acute Urinary Retention ◽  
Bladder Capacity ◽  
Inclusion Criteria ◽  
College Hospital ◽  
Medical College ◽  
High Incidence ◽  
Group A ◽  
Group B ◽  
Better Than

Objective: To compare the clean intermittent self-catheterization (CISC) with continuous indwelling catheterization (CIDC) in relieving acute urinary retention (AUR) due to benign enlargement of prostate (BEP). Materials and Methods :A total 60 patients attending in urology department of Dhaka Medical college hospital were included according to inclusion criteria ,Patients were randomized by lottery into two groups namely group –A and group –B for CISC and IDC drainage respectively . Thus total 60 patients 30 in each group completed study. Results : Most men can safely be managed as out-patients after AUR due to BPH. The degree of mucosal congestion and inflammation within the bladder was found to be lower in those using CISC and the bladder capacity in these patients was also found higher.Patients with an IDC had a high incidence of UTIs then that of patients with CISC. During the period of catheterization the incidence of UTI was 43.3% in group B in comparison to 40% in group A; before TURP 36% in group B in comparison to 10% incidence in group A.According to patient’s opinion CISC is better than IDC in the management of AUR. Experiencing bladder spasm, reporting blood in urine, management difficulties, incidence and severity of pain were less in CISC group, and the method of CISC was well accepted by patients as well as their family members. Conclusion: From the current study it may be suggested that CISC is better technique for management of AUR patient due to BPH than IDC. It can also be very helpful when surgery must be delayed or avoided due to any reasons in this group of patients. Bangladesh Journal of Urology, Vol. 21, No. 2, July 2018 p.105-110

scholarly journals Placental Mesenchymal Dysplasia: A Case Report

2012 ◽  
Vol 2012 ◽  
pp. 1-3
Author(s):  
Rachna Agarwal ◽  
Ritu Khatuja ◽  
Lipi Sharma ◽  
Alpana Singh
Keyword(s):  
Histopathological Examination ◽  
Antenatal Screening ◽  
Second Trimester ◽  
Definite Diagnosis ◽  
Patient Counseling ◽  
Partial Mole ◽  
High Incidence ◽  
Live Fetus ◽  
Placental Mesenchymal Dysplasia ◽  
Better Than

Introduction. A rare case of histologically proven placental mesenchymal dysplasia (PMD) with fetal omphalocele in a 22-year-old patient is reported.Material and Methods. Antenatal ultrasound of this patient showed hydropic placenta with a live fetus of 17 weeks period of gestation associated with omphalocele. Cordocentesis detected the diploid karyotype of the fetus. Patient, when prognosticated, choose to terminate the pregnancy in view of high incidence of fetal and placental anomalies. Subsequent histopathological examination of placenta established the diagnosis to be placental mesenchymal dysplasia.Conclusion. On clinical and ultrasonic grounds, suspicion of P.M.D. arises when hydropic placenta with a live fetus presents in second trimester of pregnancy. Cordocentesis can detect the diploid karyotype of the fetus in such cases. As this condition is prognostically better than triploid partial mole, continuation of pregnancy can sometimes be considered after through antenatal screening and patient counseling. However, a definite diagnosis of P.M.D. is made only on placental histology by absence of trophoblast hyperplasia and trophoblastic inclusions.

Influence of 8-week daily consumption of a new product combining green coffee hydroxycinnamates and beta-glucans on polyphenol bioavailability in subjects with overweight and obesity

2022 ◽  
Author(s):  
Miguel Ángel Seguido ◽  
Rosa María Tarradas ◽  
Susana González ◽  
Joaquín García-Cordero ◽  
Beatriz Sarria ◽  
...  
Keyword(s):  
Plant Extracts ◽  
New Product ◽  
Overweight And Obesity ◽  
Green Coffee ◽  
Daily Consumption ◽  
Dietary Fibres ◽  
Associated Diseases ◽  
Beta Glucans ◽  
New Strategies

Nutraceuticals based on plant extracts rich in polyphenols, as well as dietary fibres, are new strategies to fight overweight/obesity and associated diseases. However, to understand the potential effects of polyphenols...

scholarly journals Azole Resistance in Cryptococcus gattii from the Pacific Northwest: Investigation of the Role ofERG11

2013 ◽  
Vol 57 (11) ◽  
pp. 5478-5485 ◽  
Author(s):  
Charles E. Gast ◽  
Luiz R. Basso ◽  
Igor Bruzual ◽  
Brian Wong
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pacific Northwest ◽  
Cryptococcus Gattii ◽  
Azole Resistance ◽  
Western Canada ◽  
Coding Sequences ◽  
Content Type ◽  
Azole Antifungals ◽  
The Pacific ◽  
Serious Infections

ABSTRACTCryptococcus gattiiis responsible for an expanding epidemic of serious infections in Western Canada and the Northwestern United States (Pacific Northwest). Some patients with these infections respond poorly to azole antifungals, and high azole MICs have been reported in Pacific NorthwestC. gattii. In this study, multiple azoles (but not amphotericin B) had higher MICs for 25 Pacific NorthwestC. gattiithan for 34 non-Pacific NorthwestC. gattiior 20Cryptococcus neoformansstrains. We therefore examined the roles in azole resistance of overexpression of or mutations in the gene (ERG11) encoding the azole target enzyme.ERG11/ACT1mRNA ratios were higher inC. gattiithan inC. neoformans, but these ratios did not differ in Pacific Northwest and non-Pacific NorthwestC. gattiistrains, nor did they correlate with fluconazole MICs within any group. Three Pacific NorthwestC. gattiistrains with low azole MICs and 2 with high azole MICs had deduced Erg11p sequences that differed at one or more positions from that of the fully sequenced Pacific NorthwestC. gattiistrain R265. However, the azole MICs for conditionalSaccharomyces cerevisiaeerg11mutants expressing the 5 variantERG11s were within 2-fold of the azole MICs forS. cerevisiaeexpressing theERG11gene fromC. gattiiR265, non-Pacific NorthwestC. gattiistrain WM276, orC. neoformansstrains H99 or JEC21. We conclude that neitherERG11overexpression nor variations inERG11coding sequences was responsible for the high azole MICs observed for the Pacific NorthwestC. gattiistrains we studied.

A Study on the Antibacterial Property of the Extract from Apocynum Venetum

2011 ◽  
Vol 332-334 ◽  
pp. 164-167
Author(s):  
Zheng Li ◽  
Zhen Feng Dong ◽  
Jian Fei Zhang ◽  
Ji Xian Gong ◽  
Fan Jie Meng
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Antibacterial Effect ◽  
Antibacterial Property ◽  
Absolute Ethanol ◽  
Antibacterial Substance ◽  
Apocynum Venetum ◽  
Better Than

In order to determined the antibacterial property of Apocynum venetum. Three kinds of extractants (distilled water, 75% ethanol and absolute ethanol) were used to extract the leaf, stick and skin of Apocynum venetum, respectively. Flask and plate methods were used to evaluate the antibacterial property of these extracts on four kinds of classical microbiologies, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and Aspergillus niger, respectively. The results showed that the antibacterial rate of extract by 75 % ethanol was better than extracts by water or absolute ethanol on Escherichia coli and skin contained more antibacterial substance (on Escherichia coli) than others. The antibacterial rate of extract by distill water was better than extracts by 75 % ethanol or absolute ethanol on Staphylococcus aureus and stick contained more antibacterial substance (on Staphylococcus aureus) than others. Some of extracts had very weak antibacterial rate on Saccharomyces cerevisiae and all extracts had not antibacterial effect on Aspergillus niger.

scholarly journals Genetic Analysis of Azole Resistance by Transposon Mutagenesis in Saccharomyces cerevisiae

1999 ◽  
Vol 43 (11) ◽  
pp. 2731-2735 ◽  
Author(s):  
D. P. Kontoyiannis
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Targets ◽  
Molecular Mechanisms ◽  
Screening Tool ◽  
Transposon Mutagenesis ◽  
Antifungal Drugs ◽  
Azole Resistance ◽  
Recessive Mutations ◽  
Novel Drug ◽  
Novel Drug Targets

ABSTRACT The increasing resistance of Candida species to fluconazole is cause for concern. To determine the molecular mechanisms involved in resistance to fluconazole, I used a scheme of transposon mutagenesis in Saccharomyces cerevisiae, a genetically tractable yeast that is closely related to Candida albicans. This technique, which permits the generation and analysis of multiple random Tn3::LEU2::lacZfusions, can be used as a disruption mutagen (N. B. Burns et al., Genes Dev. 8:1087–1105, 1994). By using the Tn3::LEU2::lacZlibrary as a disruption mutagen, I found recessive mutations in genes that were previously found to be involved in azole resistance, e.g.,PDR5 and CPR1, and in genes previously found to be involved in azole sensitivity, e.g., ERG3. This approach also enabled me to identify recessive mutations in three genes not previously known to be involved in azole sensitivity. Two of the genes,ADA3 and SPT7, are general transcriptional regulators; the third, YMR034c, is a putative sterol transporter. Finally, by screening the Tn3::LEU2::lacZlibrary for lacZ fusions induced by a low concentration of fluconazole, I identified genes known to be induced by azoles as well as a variety of other genes not previously known to be induced by the drug. In conclusion, transposon mutagenesis is a promising screening tool for use in identifying novel drug targets and in uncovering the mechanisms involved in the response of S. cerevisiae to antifungal drugs.

scholarly journals Genetic Analysis of Azole Resistance in the Darlington Strain of Candida albicans

2000 ◽  
Vol 44 (11) ◽  
pp. 2985-2990 ◽  
Author(s):  
Hiroshi Kakeya ◽  
Yoshitsugu Miyazaki ◽  
Haruko Miyazaki ◽  
Katherine Nyswaner ◽  
Brian Grimberg ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Candida Albicans ◽  
Amino Acid ◽  
Genetic Analysis ◽  
Expression System ◽  
Single Copy ◽  
Gene Replacement ◽  
Azole Resistance ◽  
High Level

ABSTRACT High-level azole resistance in the Darlington strain ofCandida albicans was investigated by gene replacement inC. albicans and expression in Saccharomyces cerevisiae. We sequenced the ERG11 gene, which encodes the sterol C14α-demethylase, from our copy of the Darlington strain. Both alleles contained the histidine for tyrosine substitution at position 132 (Y132H) reported in Darlington by others, but we also found a threonine-for-isoleucine substitution (I471T) not previously reported in the C. albicans ERG11. The encoded I471T change in amino acids conferred azole resistance when overexpressed alone and increased azole resistance when added to the Y132H amino acid sequence in an S. cerevisiae expression system. Replacement of one copy of ERG11 in an azole-susceptible strain of C. albicans with a single copy of the Darlington ERG11 resulted in expression of the integrated copy and a modest increase in azole resistance. The profound azole resistance of the Darlington strain is the result of multiple mutations.

scholarly journals Genetic Architecture of Hsp90-Dependent Drug Resistance

2006 ◽  
Vol 5 (12) ◽  
pp. 2184-2188 ◽  
Author(s):  
Leah E. Cowen ◽  
Anne E. Carpenter ◽  
Oranart Matangkasombut ◽  
Gerald R. Fink ◽  
Susan Lindquist
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Candida Albicans ◽  
Genetic Architecture ◽  
Opportunistic Pathogen ◽  
Azole Resistance

ABSTRACT Hsp90 potentiates the evolution of azole resistance in the model yeast Saccharomyces cerevisiae and the opportunistic pathogen Candida albicans via calcineurin. Here, we explored effectors downstream of calcineurin regulating this Hsp90-dependent trait. Using S. cerevisiae erg3 mutants as a model, we determined that both Crz1 and Hph1 modulate azole resistance.

scholarly journals Primary Leiomyosarcoma of Bone: Analysis of Prognosis

Sarcoma ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
P. Brewer ◽  
V. Sumathi ◽  
R. J. Grimer ◽  
S. R. Carter ◽  
R. M. Tillman ◽  
...  
Keyword(s):  
Tumour Size ◽  
Bone Tumour ◽  
Spindle Cell Sarcoma ◽  
Rare Type ◽  
Malignant Bone Tumour ◽  
High Incidence ◽  
Primary Malignant Bone Tumour ◽  
Bone Sarcomas ◽  
Better Than ◽  
Primary Leiomyosarcoma

Leiomyosarcoma of bone is just one of the variants of spindle cell sarcoma of bone characterised by the expression of desmin and other markers indicating a significant element of smooth muscle in the tumour, without osteoid production we have investigated the management and outcome of this rare type of primary malignant bone tumour.Method. Retrospective review of data stored on a prospective database.Results. In a database of 3364 patients with primary malignant bone sarcomas, 31 patients were identified with a primary leiomyosarcoma of bone. There were 12 males and 19 females with a mean age of 46 and tumour size of 8 cm. The most common site was the distal femur followed by the proximal tibia. Treatment was with chemotherapy and surgical resection. Seven of the patients had metastases at diagnosis. Surgery was carried out in 28 patients, 8 having amputation and 20 limb salvage. Three patients developed local recurrence, but half developed metastases. All patient disease-specific survival was 57% at five years and 44% at 10 yrs but for those without metastases was 82% and 60%, respectively. The only prognostic factors were metastases at diagnosis.Conclusion. Leiomyosarcoma of bone is a very rare primary malignant bone tumour affecting a predominantly older population. Despite the high incidence of metastases, survival is better than for other bone sarcomas for those without metastases at diagnosis.

Managing Risk in a New Telecommunications Service Development Process through a Scenario Planning Approach

2002 ◽  
Vol 17 (3) ◽  
pp. 103-118 ◽  
Author(s):  
Jae-Hyeon Ahn ◽  
Ann Skudlark
Keyword(s):  
Development Process ◽  
New Product ◽  
Scenario Planning ◽  
Computer Interface ◽  
Service Development ◽  
Business Managers ◽  
Planning Approach ◽  
Managing Risk ◽  
New Strategies ◽  
Gaining Insight

Managing risk in a new product and service development process is one of the major challenges for many business managers. A scenario planning approach was incorporated into a new telecommunications service development process in order to understand the uncertainties shaping the future economic, business and technological environments. Understanding the major drivers for uncertainties helped in gaining insight and thereby generated new strategies for reducing risks and taking advantage of opportunities from uncertainty. In order to demonstrate the process and value of the approach, it was applied to a new telecommunications service concept, the Phoneweb service, which allows Internet access through telephones rather than a computer interface.

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