scholarly journals Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery

2019 ◽  
Vol 20 (16) ◽  
pp. 3958 ◽  
Author(s):  
Changhee Park ◽  
Jihye Yoo ◽  
Donghyun Lee ◽  
Seok-young Jang ◽  
Soonmin Kwon ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Blood Circulation ◽  
Tumor Tissue ◽  
Spherical Shape ◽  
Chlorin E6 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Tumor Accumulation

We fabricated poly (ethylene glycol)-block-polycaprolactone (PEG-b-PCL) nanoemulsion for drug delivery and photodynamic therapy. PEG-b-PCL effectively stabilized the interface between water and soybean oil, and the resulting nanoemulsion was about 220.3 nm in diameter with spherical shape. For photodynamic therapy (PDT), chlorin e6 (Ce6) was loaded into the nanoemulsion as a photosensitizer (PS). These chlorin e6-loaded PEG-PCL nanoemulsions (Ce6-PCL-NEs) showed efficient cellular uptake and, upon laser irradiation, generated singlet oxygen to kill tumor cells. Particularly, Ce6-PCL-NEs showed prolonged blood circulation and about 60% increased tumor accumulation compared to free Ce6 after intravenous injection to 4T1 tumor-bearing mice. These results demonstrate the promising potential of Ce6-PCL-NEs for efficient PDT and in vivo drug delivery to tumor tissue.

CD47 Functionalization of Nanoparticles as a Poly(ethylene glycol) Alternative: A Novel Approach to Improve Drug Delivery

2021 ◽  
Vol 22 ◽  
Author(s):  
Noushin Rezaei Vandchali ◽  
Fatemeh Moadab ◽  
Eskandar Taghizadeh ◽  
Amir Tajbakhsh ◽  
Seyed Mohammad Gheibi-hayat
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Retention Time ◽  
Blood Circulation ◽  
Regulatory Protein ◽  
Reticuloendothelial System ◽  
Poly Ethylene Glycol ◽  
Drug Delivery Vehicles ◽  
Novel Approach ◽  
Poly Ethylene

Abstract: Bio-degradable nanoparticles (NPs) have several utilizations as the drug delivery vehicles due to their acceptable bio-availability, lower toxicity, potency for encapsulation and controlled release. Moreover, interaction of the NPs with the macrophages of reticuloendothelial system (RES) may decrease NPs efficacy for medical purposes. The surface of NPs is conventionally neutralized with the molecules such as poly(ethylene glycol) (PEG), as one of the most widely applied stealth polymers, in order to restrict the NPs clearance through the RES system. In fact, these molecules exhibit resistance to the RES clearance and proteins adsorption. It is unfortunate that modifying the PEG has some shortcomings like problems in the synthesis as well as correlation to the immune reaction. The CD47 receptor has been well known as a ‘don’t-eat-me’ molecule on the self-cells' surface. Therefore, the receptor will inhibit phagocytosis via binding to its ligand that is known as the signal regulatory protein α (SIRP-α). Moreover, the CD47 receptor, as one of the biomimetic substances, or its derivative peptides have been used recently on the surface of nanoparticles to inhibit phagocytosis and increase the NPs retention time in the blood circulation. Therefore, this review study examined the CD47 receptor and its role in the immune system as well as the use of the CD47 receptor in coating NPs to increase their retention time in the blood circulation.

Development of chlorin e6-conjugated poly(ethylene glycol)-poly(d,l-lactide) nanoparticles for photodynamic therapy

Nanomedicine ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. 819-834 ◽  
Author(s):  
Preeti Kumari ◽  
Sri Vishnu Kiran Rompicharla ◽  
Himanshu Bhatt ◽  
Balaram Ghosh ◽  
Swati Biswas
Keyword(s):  
Photodynamic Therapy ◽  
Ethylene Glycol ◽  
Chlorin E6 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

A smart drug-delivery nanosystem based on carboxylated graphene quantum dots for tumor-targeted chemotherapy

Nanomedicine ◽  
2019 ◽  
Vol 14 (15) ◽  
pp. 2011-2025 ◽  
Author(s):  
Zhen Li ◽  
Jialong Fan ◽  
Chunyi Tong ◽  
Hongyan Zhou ◽  
Wenmiao Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Quantum Dots ◽  
Folic Acid ◽  
Ethylene Glycol ◽  
Drug Delivery System ◽  
Delivery System ◽  
Graphene Quantum Dots ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Aim: Constructing a new drug-delivery system using carboxylated graphene quantum dots (cGQDs) for tumor chemotherapy in vivo. Materials & methods: A drug-delivery system was synthesized through a crosslink reaction of cGQDs, NH2-poly(ethylene glycol)-NH2 and folic acid. Results: A drug delivery system of folic acid-poly(ethylene glycol)-cGQDs was successfully constructed with ideal entrapment efficiency (97.5%) and drug-loading capacity (40.1%). Cell image indicated that the nanosystem entered into human cervical cancer cells mainly through macropinocytosis-dependent pathway. In vivo experiments showed the outstanding antitumor ability and low systemic toxicity of this nanodrug-delivery system. Conclusion: The newly developed drug-delivery system provides an important alternative for tumor therapy without causing systemic adverse effects.

Chlorin e6 Conjugated Methoxy-Poly(Ethylene Glycol)-Poly(D,L-Lactide) Glutathione Sensitive Micelles for Photodynamic Therapy

2020 ◽  
Vol 37 (2) ◽  
Author(s):  
Preeti Kumari ◽  
Milan Paul ◽  
Himanshu Bhatt ◽  
Sri Vishnu Kiran Rompicharla ◽  
Debolina Sarkar ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Ethylene Glycol ◽  
Chlorin E6 ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

A Study on PELGE Nanoparticles as Controlled Drug Delivery Systems for Intravenous

2005 ◽  
Vol 288-289 ◽  
pp. 163-166 ◽  
Author(s):  
You Rong Duan ◽  
W.S. Liu ◽  
J. Liu ◽  
Z.R. Zhang
Keyword(s):  
Ethylene Glycol ◽  
Blood Circulation ◽  
Controlled Drug Delivery ◽  
Drug Carriers ◽  
Circulation Time ◽  
Poly Ethylene Glycol ◽  
Systemic Blood ◽  
Model Drug ◽  
Poly Ethylene

The objective of this study was to evaluate the in vivo characteristics of poly (ethylene glycol)-poly (lacticacid-co-glycolicacid)-poly (ethylene- glycol) (PELGE) copolymers as drug carriers. In order to test this circulation time, mitoxantrone (DHAQ) was used as a model drug in this study. DHAQ nanoparticles (DHAQ-NP) were prepared, subsequently the DHAQ-NP were evaluated by measuring the drug concentration in plasma after intravenous administration via the tail vein of mice. The circulation time of the DHAQ-NP were tested. The results showed prolonged mitoxantrone (DHAQ) residence in systemic blood circulation.

scholarly journals Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2190
Author(s):  
Howard Kim ◽  
Mi Woon Kim ◽  
Young-IL Jeong ◽  
Hoe Saeng Yang
Keyword(s):  
Cervical Cancer ◽  
Photodynamic Therapy ◽  
Hela Cells ◽  
Folate Receptor ◽  
Chlorin E6 ◽  
Ros Generation ◽  
Cervical Cancer Cells ◽  
Poly Ethylene

The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (H2O2) and particle size distribution changed from monomodal distribution pattern to multimodal pattern. The fluorescence intensity and Ce6 release rate also increased due to the increase in H2O2 concentration, indicating that the nanophotosensitizers displayed ROS sensitivity. The Ce6 uptake ratio, ROS generation and cell cytotoxicity of the nanophotosensitizers were significantly higher than those of the Ce6 itself against HeLa cells in vitro. Furthermore, the nanophotosensitizers showed folate-receptor-specific delivery capacity and phototoxicity. The intracellular delivery of nanophotosensitizers was inhibited by folate receptor blocking, indicating that they have folate-receptor specificity in vitro and in vivo. Nanophotosensitizers showed higher efficiency in inhibition of tumor growth of HeLa cells in vivo compared to Ce6 alone. These results show that nanophotosensitizers of FaPEGbCDseseCe6 conjugates are promising candidates as PDT of cervical cancer.

scholarly journals The Importance of Poly(ethylene glycol) Alternatives for Overcoming PEG Immunogenicity in Drug Delivery and Bioconjugation

Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 298 ◽  
Author(s):  
Thai Thanh Hoang Thi ◽  
Emily H. Pilkington ◽  
Dai Hai Nguyen ◽  
Jung Seok Lee ◽  
Ki Dong Park ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Blood Circulation ◽  
Drug Efficacy ◽  
Poly Ethylene Glycol ◽  
Life Threatening ◽  
Poly Ethylene ◽  
Interfering Rna ◽  
Accelerated Blood Clearance ◽  
Established Technique

Poly(ethylene glycol) (PEG) is widely used as a gold standard in bioconjugation and nanomedicine to prolong blood circulation time and improve drug efficacy. The conjugation of PEG to proteins, peptides, oligonucleotides (DNA, small interfering RNA (siRNA), microRNA (miRNA)) and nanoparticles is a well-established technique known as PEGylation, with PEGylated products have been using in clinics for the last few decades. However, it is increasingly recognized that treating patients with PEGylated drugs can lead to the formation of antibodies that specifically recognize and bind to PEG (i.e., anti-PEG antibodies). Anti-PEG antibodies are also found in patients who have never been treated with PEGylated drugs but have consumed products containing PEG. Consequently, treating patients who have acquired anti-PEG antibodies with PEGylated drugs results in accelerated blood clearance, low drug efficacy, hypersensitivity, and, in some cases, life-threatening side effects. In this succinct review, we collate recent literature to draw the attention of polymer chemists to the issue of PEG immunogenicity in drug delivery and bioconjugation, thereby highlighting the importance of developing alternative polymers to replace PEG. Several promising yet imperfect alternatives to PEG are also discussed. To achieve asatisfactory alternative, further joint efforts of polymer chemists and scientists in related fields are urgently needed to design, synthesize and evaluate new alternatives to PEG.

Regulating the surface poly(ethylene glycol) density of polymeric nanoparticles and evaluating its role in drug delivery in vivo

Biomaterials ◽  
2015 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Xiao-Jiao Du ◽  
Ji-Long Wang ◽  
Wei-Wei Liu ◽  
Jin-Xian Yang ◽  
Chun-Yang Sun ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Polymeric Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

The effect of surface poly(ethylene glycol) length on in vivo drug delivery behaviors of polymeric nanoparticles

Biomaterials ◽  
2018 ◽  
Vol 182 ◽  
pp. 104-113 ◽  
Author(s):  
Ji-Long Wang ◽  
Xiao-Jiao Du ◽  
Jin-Xian Yang ◽  
Song Shen ◽  
Hong-Jun Li ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Ethylene Glycol ◽  
Polymeric Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Effect Of Surface

scholarly journals Multifunctional nanoplatforms as cascade-responsive drug-delivery carriers for effective synergistic chemo-photodynamic cancer treatment

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Fan Li ◽  
Yan Liang ◽  
Miaochen Wang ◽  
Xing Xu ◽  
Fen Zhao ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Blood Circulation ◽  
The Novel ◽  
Hydrophobic Core ◽  
Cancer Suppression ◽  
532 Nm

AbstractSynergistic chemo-photodynamic therapy has garnered attention in the field of cancer treatment. Here, a pH cascade-responsive micellar nanoplatform with nucleus-targeted ability, for effective synergistic chemo-photodynamic cancer treatment, was fabricated. In this micellar nanoplatform, 5-(4-carboxyphenyl)-10,15,20-triphenylporphyrin (Por), a photodynamic therapy (PDT) agent was utilized for carrying the novel anticancer drug GNA002 to construct a hydrophobic core, and cyclic RGD peptide (cRGD)-modified polyethylene glycol (PEG) (cRGD-PEG) connected the cell-penetrating peptide hexaarginine (R6) through a pH-responsive hydrazone bond (cRGD-PEG-N = CH-R6) to serve as a hydrophilic shell for increasing blood circulation time. After passively accumulating in tumor sites, the self-assembled GNA002-loaded nanoparticles were actively internalized into cancer cells via the cRGD ligands. Once phagocytosed by lysosomes, the acidity-triggered detachment of the cRGD-PEG shell led to the formation of R6-coated secondary nanoparticles and subsequent R6-mediated nucleus-targeted drug delivery. Combined with GNA002-induced nucleus-specific chemotherapy, reactive oxygen species produced by Por under 532-nm laser irradiation achieved a potent synergistic chemo-photodynamic cancer treatment. Moreover, our in vitro and in vivo anticancer investigations revealed high cancer-suppression efficacy of this ideal multifunctional nanoplatform, indicating that it could be a promising candidate for synergistic anticancer therapy.

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