scholarly journals C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency

2019 ◽  
Vol 20 (22) ◽  
pp. 5611
Author(s):  
Wajeeha Jamil ◽  
Wei Wu ◽  
Hui Gong ◽  
Jing-Wen Huang ◽  
Mudassar Ahmad ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Zinc Finger Proteins ◽  
Bimolecular Fluorescence Complementation ◽  
Luciferase Assay ◽  
Response Factors ◽  
High Co2 ◽  
Persimmon Fruit ◽  
Genes Encoding

Hypoxic environments are generally undesirable for most plants, but for astringent persimmon, high CO2 treatment (CO2 > 90%), also termed artificial high-CO2 atmosphere (AHCA), causes acetaldehyde accumulation and precipitation of soluble tannins and could remove astringency. The multiple transcriptional regulatory linkages involved in persimmon fruit deastringency have been advanced significantly by characterizing the ethylene response factors (ERFs), WRKY and MYB; however, the involvement of zinc finger proteins for deastringency has not been investigated. In this study, five genes encoding C2H2-type zinc finger proteins were isolated and designed as DkZF1-5. Phylogenetic and sequence analyses suggested the five DkZFs could be clustered into two different subgroups. qPCR analysis indicated that transcript abundances of DkZF1/4 were significantly upregulated during AHCA treatment (1% O2 and 95% CO2) at day 1, DkZF2/5 at both day 1 and 2, while DkZF3 at day 2. Dual-luciferase assay indicated DkZF1 and DkZF2 as the activators of deastringency-related structural genes (DkPDC2 and DkADH1) and transcription factors (DkERF9/10). Moreover, combinative effects between various transcription factors were investigated, indicating that DkZF1 and DkZF2 synergistically showed significantly stronger activations on the DkPDC2 promoter. Further, both bimolecular fluorescence complementation (BiFC) and yeast two hybrid (Y2H) assays confirmed that DkZF2 had protein–protein interactions with DkZF1. Thus, these findings illustrate the regulatory mechanisms of zinc finger proteins for persimmon fruit deastringency under AHCA.

scholarly journals High CO2/hypoxia-induced softening of persimmon fruit is modulated by DkERF8/16 and DkNAC9 complexes

2020 ◽  
Vol 71 (9) ◽  
pp. 2690-2700 ◽  
Author(s):  
Wei Wu ◽  
Miao-miao Wang ◽  
Hui Gong ◽  
Xiao-fen Liu ◽  
Da-long Guo ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Synergistic Effects ◽  
Bimolecular Fluorescence Complementation ◽  
Diospyros Kaki ◽  
Xyloglucan Endotransglycosylase ◽  
Mobility Shift ◽  
Response Factors ◽  
High Co2 ◽  
Persimmon Fruit ◽  
Co2 Treatment

Abstract Most persimmon (Diospyros kaki) cultivars are astringent and require post-harvest deastringency treatments such as 95% CO2 (high-CO2 treatment) to make them acceptable to consumers. High-CO2 treatment can, however, also induce excessive softening, which can be reduced by adding 1-methylcyclopropene (1-MCP). Previous studies have shown that genes encoding the ETHYLENE RESPONSE FACTORS (ERFs) DkERF8/16/19 can trans-activate xyloglucan endotransglycosylase/hydrolase (DkXTH9), which encodes the cell wall-degrading enzyme associated with persimmon fruit softening. In this study, RNA-seq data between three treatments were compared, namely high-CO2, high-CO2+1-MCP, and controls. A total of 227 differentially expressed genes, including 17 transcription factors, were predicted to be related to persimmon post-deastringency softening. Dual-luciferase assays indicated that DkNAC9 activated the DkEGase1 promoter 2.64-fold. Synergistic effects on transcription of DkEGase1 that involved DkNAC9 and the previously reported DkERF8/16 were identified. Electrophoretic mobility shift assay indicated that DkNAC9 could physically bind to the DkEGase1 promoter. Bimolecular fluorescence complementation and firefly luciferase complementation imaging assays indicated protein–protein interactions between DkNAC9 and DkERF8/16. Based on these findings, we conclude that DkNAC9 is a direct transcriptional activator of DkEGase1 that can co-operate with DkERF8/16 to enhance fruit post-deastringency softening.

Single zinc-finger extension: enhancing transcriptional activity and specificity of three-zinc-finger proteins

2005 ◽  
Vol 386 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Alexander E.F. Smith ◽  
Farzin Farzaneh ◽  
Kevin G. Ford
Keyword(s):  
Transcription Factors ◽  
Fusion Protein ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Zinc Finger Proteins ◽  
Finger Protein ◽  
Finger Extension ◽  
Vp16 Fusion

AbstractIn order to demonstrate that an existing zinc-finger protein can be simply modified to enhance DNA binding and sequence discrimination in both episomal and chromatin contexts using existing zinc-finger DNA recognition code data, and without recourse to phage display and selection strategies, we have examined the consequences of a single zinc-finger extension to a synthetic three-zinc-finger VP16 fusion protein, on transcriptional activation from model target promoters harbouring the zinc-finger binding sequences. We report a nearly 10-fold enhanced transcriptional activation by the four-zinc-finger VP16 fusion protein relative to the progenitor three-finger VP16 protein in transient assays and a greater than five-fold enhancement in stable reporter-gene expression assays. A marked decrease in transcriptional activation was evident for the four-zinc-finger derivative from mutated regulatory regions compared to the progenitor protein, as a result of recognition site-size extension. This discriminatory effect was shown to be protein concentration-dependent. These observations suggest that four-zinc-finger proteins are stable functional motifs that can be a significant improvement over the progenitor three-zinc-finger protein, both in terms of specificity and the ability to target transcriptional function to promoters, and that single zinc-finger extension can therefore have a significant impact on DNA zinc-finger protein interactions. This is a simple route for modifying or enhancing the binding properties of existing synthetic zinc-finger-based transcription factors and may be particularly suited for the modification of endogenous zinc-finger transcription factors for promoter biasing applications.

Focal brain injury induces multiple immediate early genes encoding zinc finger transcription factors

1995 ◽  
Vol 28 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Jari Honkaniemi ◽  
Stephen M. Sagar ◽  
Ilkka Pyykönen ◽  
Kathleen J. Hicks ◽  
Frank R. Sharp
Keyword(s):  
Transcription Factors ◽  
Brain Injury ◽  
Zinc Finger ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
Early Genes ◽  
Focal Brain Injury ◽  
Genes Encoding

scholarly journals Identification of genes encoding zinc finger proteins, non-histone chromosomal HMG protein homologue, and a putative GTP phosphohydrolase in the genome of Chilo iridescent virus

1994 ◽  
Vol 22 (2) ◽  
pp. 158-166 ◽  
Author(s):  
Paul Schnitzler ◽  
Michael Hug ◽  
Michaela Handermann ◽  
Waltraud Janssen ◽  
Eugene V. Koonin ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Chilo Iridescent Virus ◽  
Iridescent Virus ◽  
Genes Encoding ◽  
Hmg Protein

Three Genes Encoding Zinc Finger Proteins on Human Chromosome 6p21.3: Members of a New Subclass of the Krüppel Gene Family Containing the Conserved SCAN Box Domain

Genomics ◽  
1997 ◽  
Vol 43 (2) ◽  
pp. 191-201 ◽  
Author(s):  
Pauline L. Lee ◽  
Terri Gelbart ◽  
Carol West ◽  
Michele Adams ◽  
Ryan Blackstone ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Gene Family ◽  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Genes Encoding ◽  
Chromosome 6P21.3

scholarly journals Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains.

1995 ◽  
Vol 15 (5) ◽  
pp. 2448-2456 ◽  
Author(s):  
M Crossley ◽  
M Merika ◽  
S H Orkin
Keyword(s):  
Transcription Factors ◽  
Dna Binding ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Physical Interaction ◽  
Erythroid Cells ◽  
Protein Protein Interactions ◽  
Self Association ◽  
Zinc Finger Region ◽  
In Erythroid Cells

GATA-1, the founding member of a distinctive family of transcription factors, is expressed predominantly in erythroid cells and participates in the expression of numerous erythroid cell-expressed genes. GATA-binding sites are found in the promoters and enhancers of globin and nonglobin erythroid genes as well as in the alpha- and beta-globin locus control regions. To elucidate how GATA-1 may function in a variety of regulatory contexts, we have examined its protein-protein interactions. Here we show that GATA-1 self-associates in solution and in whole-cell extracts and that the zinc finger region of the molecule is sufficient to mediate this interaction. This physical interaction can influence transcription, as GATA-1 self-association is able to recruit a transcriptionally active but DNA-binding-defective derivative of GATA-1 to promoter-bound GATA-1 and result in superactivation. Through in vitro studies with bacterially expressed glutathione S-transferase fusion proteins, we have localized the minimal domain required for GATA-1 self-association to 40 amino acid residues within the C-terminal zinc finger region. Finally, we have detected physical interaction of GATA-1 with other GATA family members (GATA-2 and GATA-3) also mediated through the zinc finger domain. These findings have broad implications for the involvement of GATA factors in transcriptional control. In particular, the interaction of GATA-1 with itself and with other transcription factors may facilitate its function at diverse promoters in erythroid cells and also serve to bring together, or stabilize, loops between distant regulatory elements, such as the globin locus control regions and downstream globin promoters. We suggest that the zinc finger region of GATA-1, and related proteins, is multifunctional and mediates not only DNA binding but also important protein-protein interactions.

scholarly journals Transcriptome Analysis of Lolium temulentum Exposed to a Combination of Drought and Heat Stress

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2247
Author(s):  
Ruth C. Martin ◽  
Brent A. Kronmiller ◽  
James E. Dombrowski
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Transcriptome Analysis ◽  
Transcriptional Control ◽  
Leucine Zipper ◽  
Multiple Stressors ◽  
Response Factors ◽  
Basic Leucine Zipper ◽  
Auxin Response Factors ◽  
Lolium Temulentum

Drought and heat are two major stresses predicted to increase in the future due to climate change. Plants exposed to multiple stressors elicit unique responses from those observed under individual stresses. A comparative transcriptome analysis of Lolium temulentum exposed to drought plus heat and non-stressed control plants revealed 20,221 unique up-regulated and 17,034 unique down-regulated differentially regulated transcripts. Gene ontology analysis revealed a strong emphasis on transcriptional regulation, protein folding, cell cycle/parts, organelles, binding, transport, signaling, oxidoreductase, and antioxidant activity. Differentially expressed genes (DEGs) encoding for transcriptional control proteins such as basic leucine zipper, APETALA2/Ethylene Responsive Factor, NAC, and WRKY transcription factors, and Zinc Finger (CCCH type and others) proteins were more often up-regulated, while DEGs encoding Basic Helix-Loop-Helix, MYB and GATA transcription factors, and C2H2 type Zinc Finger proteins were more often down-regulated. The DEGs encoding heat shock transcription factors were only up-regulated. Of the hormones, auxin-related DEGs were the most prevalent, encoding for auxin response factors, binding proteins, and efflux/influx carriers. Gibberellin-, cytokinin- and ABA-related DEGs were also prevalent, with fewer DEGs related to jasmonates and brassinosteroids. Knowledge of genes/pathways that grasses use to respond to the combination of heat/drought will be useful in developing multi-stress resistant grasses.

scholarly journals Zinc Finger Proteins as Designer Transcription Factors

2000 ◽  
Vol 275 (12) ◽  
pp. 8742-8748 ◽  
Author(s):  
Jong Seok Kang ◽  
Jin-Soo Kim
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Proteins

scholarly journals Generation of a versatile BiFC ORFeome library for analyzing protein-protein interactions in live Drosophila

2018 ◽  
Author(s):  
J. Bischof ◽  
M. Duffraisse ◽  
E. Furger ◽  
L. Ajuria ◽  
G. Giraud ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Protein Interactions ◽  
Large Scale ◽  
Bimolecular Fluorescence Complementation ◽  
Protein Protein Interactions ◽  
Expression Domain ◽  
Hox Proteins ◽  
Non Invasive ◽  
Tripartite Interactions

AbstractTranscription factors achieve specificity by establishing intricate interaction networks that will change depending on the cell context. Capturing these interactions in live condition is however a challenging issue that requires sensitive and non-invasive methods. We present a set of fly lines, called “multicolor BiFC library”, which covers most of the Drosophila transcription factors for performing Bimolecular Fluorescence Complementation (BiFC). The multicolor BiFC library can be used to probe binary or tripartite interactions and is compatible for large-scale interaction screens. The library can also be coupled with established Drosophila genetic resources to analyze interactions in the developmentally relevant expression domain of each protein partner. We provide proof of principle experiments of these various applications, using Hox proteins in the live Drosophila embryo as a case study. Overall this novel collection of ready-to-use fly lines constitutes an unprecedented genetic toolbox for the identification and analysis of protein-protein interactions in vivo.

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