scholarly journals Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

2020 ◽  
Vol 21 (6) ◽  
pp. 2062
Author(s):  
Magdalena Wójcik-Jagła ◽  
Marcin Rapacz ◽  
Ewa Dubas ◽  
Monika Krzewska ◽  
Przemysław Kopeć ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
Doubled Haploid ◽  
Triosephosphate Isomerase ◽  
Elongation Factor ◽  
Proteome Analysis ◽  
Stress Factors ◽  
Plant Tolerance ◽  
Doubled Haploid Lines ◽  
Dh Lines

Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.

Recurrent selection in doubled-haploid populations of barley (Hordeurn vulgare L.)

1985 ◽  
Vol 27 (2) ◽  
pp. 172-177 ◽  
Author(s):  
J. D. Patel ◽  
E. Reinbergs ◽  
S. O. Fejer
Keyword(s):  
Doubled Haploid ◽  
Recurrent Selection ◽  
Doubled Haploids ◽  
Additive Genetic Variance ◽  
Response To Selection ◽  
Hordeum Vulgare L ◽  
Doubled Haploid Lines ◽  
Days To Heading ◽  
High Selection ◽  
Dh Lines

Cycle zero (C0) of recurrent selection in barley (Hordeum vulgare L.) was initiated by diallel mating of seven highly selected parents. A total of 398 doubled-haploid (C0DH) lines were derived from 21 crosses and were evaluated along with their parents in C0 experiment. Seven doubled-haploid lines (DH) were selected from the cycle zero (C0) experiment and intercrossed to form cycle 1 (C1). From the 21 crosses of the diallel, 260 doubled-haploid lines (C1DH) were derived and were evaluated along with the C0 and C1 parents. The frequency distribution of the standardized means of the DH lines from C0 and C1 indicated a slight response to selection for seed yield. Genetic analysis of the C1DH population showed high additive genetic variance for yield per hill, plant height, and yield per spike, and a high proportion of additive × additive epistasis for spikes per hill, days to heading, and 100-seed weight. Seven doubled-haploid lines were selected from different high-yielding crosses represented by C1DH lines. High selection pressure was applied for yield per hill, yield per spike, and spikes per hill. Further response to selection is expected in later cycles. The seven selected doubled-haploid lines will be used as the parents of the next recurrent selection cycle.Key words: recurrent selection, doubled haploids, additive, epistasis, heritability, Hordeum.

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

scholarly journals Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage

Genetika ◽  
2017 ◽  
Vol 49 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Jahad Soorni ◽  
Seyed Kazemitabar ◽  
Danial Kahrizi ◽  
Ali Dehestani ◽  
Nadali Bagheri
Keyword(s):  
Freezing Tolerance ◽  
Doubled Haploid ◽  
Large Scale ◽  
Principal Component ◽  
Camelina Sativa ◽  
Stress Conditions ◽  
Freezing Stress ◽  
Doubled Haploid Lines ◽  
Survival Percentage ◽  
Dh Lines

Freezing stress is an important abiotic stress that limiting the yield and the spatial distribution of many important crops. This study was undertaken to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.) along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and Cascade) as experimental control under freezing stress conditions (-14?C for 6 h) to identify lines with high or low level of tolerance to freezing stress for further studies. First, a protocol was developed for large scale screening of camelina germplasm under freezing stress conditions. For this purpose, an experiment with different freezing temperatures (-5, -10, -15 and -20?C) was conducted to find an appropriate temperature that discriminated best between genotypes (i.e. the LT50 temperature). The LT50 values for camelina lines were varied between -10.2 and -17.1?C with an average of -13.94?C for all of the camelina lines. Therefore, we selected the -14?C exposure for 6 h as an appropriate temperature to screening of camelina lines. The principal components of measured parameters (LT50, survival percentage, relative conductivity and scoring) was using principal component analysis that determine freezing-tolerant and freezing-sensitive lines. Among 136 doubled haploid lines, some lines (58, 62 and 101) had higher level of freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing sensitive. The selected lines in a preliminary freezing screening are useful for further evaluations.

scholarly journals Assessment of molecular diversity in doubled haploid lines of Camelina (Camelina sativa), as a new emerging oil crop

2021 ◽  
Author(s):  
Mozafar Sadeghikian ◽  
Abdollah Najaphy ◽  
Danial Kahrizi ◽  
Hossein Rostami-Ahmadvandi
Keyword(s):  
Genetic Variation ◽  
Doubled Haploid ◽  
Crop Improvement ◽  
Issr Markers ◽  
Camelina Sativa ◽  
Resolving Power ◽  
Oilseed Crop ◽  
Oil Composition ◽  
Doubled Haploid Lines ◽  
Dh Lines

Abstract Camelina [Camelina sativa (L.) Crantz], an oilseed crop, belongs to Brassicaceae family. Two unique features of camelina in comparison with the main oil crops are adaptation to different environments and also its unique oil composition. Development of doubled haploid plants is one of the essential methods for the crop improvement. This research was conducted to evaluate the genetic variation of 81 Camelina doubled haploid (DH) lines obtained from fifteen crosses by ISSR markers. The total number of amplified bands was 243, of which 239 bands (98.3%) showed polymorphism. The percentage of polymorphic bands (PPB) varied between 93.75 and 100. The size of the bands ranged from 50 to 1,700 base pairs. The informative ISSRs were identified by estimating marker features: polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). Three markers had higher RP values (9.88, 8.5 and 7.46) and were the most informative markers to identify the DH lines. Cluster analysis based on Complete algorithm divided the lines into five groups, indicating relatively clear configuration from the geographic distribution patterns of the parents of the doubled haploid lines. Principal coordinate analysis (PCoA) classified the 81 camelina DH lines into six groups. The lines grouping by these two methods was similar to each other. The ISSR markers detected high polymorphism to reveal genetic variation of camelina DH lines. The findings of this research, along with biochemical traits, can improve classical and molecular breeding programs of camelina.

scholarly journals Proteome Analysis of Date Palm (Phoenix dactylifera L.) under Severe Drought and Salt Stress

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Abdulrahman L. Al-Malki ◽  
Khalid O. Abulnaja
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Date Palm ◽  
Proteome Analysis ◽  
Rubisco Activase ◽  
2D Electrophoresis ◽  
Severe Drought ◽  
Protein Abundance ◽  
Low Protein

Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

scholarly journals Transcriptomic analysis of sweet potato under dehydration stress identifies candidate genes for drought tolerance

Plant Direct ◽  
2018 ◽  
Vol 2 (10) ◽  
pp. e00092 ◽  
Author(s):  
Kin H. Lau ◽  
María del Rosario Herrera ◽  
Emily Crisovan ◽  
Shan Wu ◽  
Zhangjun Fei ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
Sweet Potato ◽  
Transcriptomic Analysis ◽  
Dehydration Stress

scholarly journals Trehalose improves cell proliferation and dehydration tolerance of human HaCaT cells

2015 ◽  
Vol 67 (3) ◽  
pp. 849-860
Author(s):  
Kyung Lee ◽  
Doo Moon ◽  
Sang Kang
Keyword(s):  
Cell Proliferation ◽  
Tryptophan Hydroxylase ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Elongation Factor ◽  
Proteome Analysis ◽  
Hacat Cells ◽  
Dehydration Tolerance ◽  
Vitamin D Receptors ◽  
P21 Activated Kinase

Trehalose is a disaccharide molecule that serves as a natural osmotic regulator in halophilic microorganisms and plants but not in mammals. We observed that human HaCaT cells supplied with trehalose improved cell proliferation and extended viability under dehydration. In HaCaT cells, in response to increasing concentrations of exogenous trehalose, the levels of heat shock protein (HSP) 70 increased and matrix metalloproteinase (MMP) 1 decreased. Proteome analysis of trehalose-treated HaCaT cells revealed remarkable increases in the levels of proteins involved in cell signaling and the cell cycle, including p21 activated kinase I, Sec I family domain protein and elongation factor G. Moreover, the proteins for cell stress resistance, tryptophan hydroxylase, serine/cysteine proteinase inhibitors and vitamin D receptors were also increased. In addition, the proteins responsible for the maintenance of the cytoskeleton and cellular structures including procollagen-lysine dioxygenase, vinculin and ezrin were increased. Proteomic data revealed that trehalose affected HaCaT cells by inducing the proteins involved in cell proliferation. These results suggest that trehalose improves the proliferation and dehydration tolerance of HaCaT cells by inducing proteins involved in cell growth and dehydration protection.

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