scholarly journals Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage

Genetika ◽  
2017 ◽  
Vol 49 (1) ◽  
pp. 173-181 ◽  
Author(s):  
Jahad Soorni ◽  
Seyed Kazemitabar ◽  
Danial Kahrizi ◽  
Ali Dehestani ◽  
Nadali Bagheri
Keyword(s):  
Freezing Tolerance ◽  
Doubled Haploid ◽  
Large Scale ◽  
Principal Component ◽  
Camelina Sativa ◽  
Stress Conditions ◽  
Freezing Stress ◽  
Doubled Haploid Lines ◽  
Survival Percentage ◽  
Dh Lines

Freezing stress is an important abiotic stress that limiting the yield and the spatial distribution of many important crops. This study was undertaken to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.) along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and Cascade) as experimental control under freezing stress conditions (-14?C for 6 h) to identify lines with high or low level of tolerance to freezing stress for further studies. First, a protocol was developed for large scale screening of camelina germplasm under freezing stress conditions. For this purpose, an experiment with different freezing temperatures (-5, -10, -15 and -20?C) was conducted to find an appropriate temperature that discriminated best between genotypes (i.e. the LT50 temperature). The LT50 values for camelina lines were varied between -10.2 and -17.1?C with an average of -13.94?C for all of the camelina lines. Therefore, we selected the -14?C exposure for 6 h as an appropriate temperature to screening of camelina lines. The principal components of measured parameters (LT50, survival percentage, relative conductivity and scoring) was using principal component analysis that determine freezing-tolerant and freezing-sensitive lines. Among 136 doubled haploid lines, some lines (58, 62 and 101) had higher level of freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing sensitive. The selected lines in a preliminary freezing screening are useful for further evaluations.

scholarly journals Assessment of molecular diversity in doubled haploid lines of Camelina (Camelina sativa), as a new emerging oil crop

2021 ◽  
Author(s):  
Mozafar Sadeghikian ◽  
Abdollah Najaphy ◽  
Danial Kahrizi ◽  
Hossein Rostami-Ahmadvandi
Keyword(s):  
Genetic Variation ◽  
Doubled Haploid ◽  
Crop Improvement ◽  
Issr Markers ◽  
Camelina Sativa ◽  
Resolving Power ◽  
Oilseed Crop ◽  
Oil Composition ◽  
Doubled Haploid Lines ◽  
Dh Lines

Abstract Camelina [Camelina sativa (L.) Crantz], an oilseed crop, belongs to Brassicaceae family. Two unique features of camelina in comparison with the main oil crops are adaptation to different environments and also its unique oil composition. Development of doubled haploid plants is one of the essential methods for the crop improvement. This research was conducted to evaluate the genetic variation of 81 Camelina doubled haploid (DH) lines obtained from fifteen crosses by ISSR markers. The total number of amplified bands was 243, of which 239 bands (98.3%) showed polymorphism. The percentage of polymorphic bands (PPB) varied between 93.75 and 100. The size of the bands ranged from 50 to 1,700 base pairs. The informative ISSRs were identified by estimating marker features: polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). Three markers had higher RP values (9.88, 8.5 and 7.46) and were the most informative markers to identify the DH lines. Cluster analysis based on Complete algorithm divided the lines into five groups, indicating relatively clear configuration from the geographic distribution patterns of the parents of the doubled haploid lines. Principal coordinate analysis (PCoA) classified the 81 camelina DH lines into six groups. The lines grouping by these two methods was similar to each other. The ISSR markers detected high polymorphism to reveal genetic variation of camelina DH lines. The findings of this research, along with biochemical traits, can improve classical and molecular breeding programs of camelina.

Recurrent selection in doubled-haploid populations of barley (Hordeurn vulgare L.)

1985 ◽  
Vol 27 (2) ◽  
pp. 172-177 ◽  
Author(s):  
J. D. Patel ◽  
E. Reinbergs ◽  
S. O. Fejer
Keyword(s):  
Doubled Haploid ◽  
Recurrent Selection ◽  
Doubled Haploids ◽  
Additive Genetic Variance ◽  
Response To Selection ◽  
Hordeum Vulgare L ◽  
Doubled Haploid Lines ◽  
Days To Heading ◽  
High Selection ◽  
Dh Lines

Cycle zero (C0) of recurrent selection in barley (Hordeum vulgare L.) was initiated by diallel mating of seven highly selected parents. A total of 398 doubled-haploid (C0DH) lines were derived from 21 crosses and were evaluated along with their parents in C0 experiment. Seven doubled-haploid lines (DH) were selected from the cycle zero (C0) experiment and intercrossed to form cycle 1 (C1). From the 21 crosses of the diallel, 260 doubled-haploid lines (C1DH) were derived and were evaluated along with the C0 and C1 parents. The frequency distribution of the standardized means of the DH lines from C0 and C1 indicated a slight response to selection for seed yield. Genetic analysis of the C1DH population showed high additive genetic variance for yield per hill, plant height, and yield per spike, and a high proportion of additive × additive epistasis for spikes per hill, days to heading, and 100-seed weight. Seven doubled-haploid lines were selected from different high-yielding crosses represented by C1DH lines. High selection pressure was applied for yield per hill, yield per spike, and spikes per hill. Further response to selection is expected in later cycles. The seven selected doubled-haploid lines will be used as the parents of the next recurrent selection cycle.Key words: recurrent selection, doubled haploids, additive, epistasis, heritability, Hordeum.

scholarly journals Evaluation of Genetic Variation and Parameters of Fatty Acid Profile in Doubled Haploid Lines of Camelina sativa L.

2020 ◽  
Vol 6 (2) ◽  
pp. 79-96
Author(s):  
Farshad Fallah ◽  
Danial Kahrizi ◽  
Abbas Rezaeizad ◽  
Alireza Zebarzadi ◽  
Lila Zarei ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Doubled Haploid ◽  
Camelina Sativa ◽  
Doubled Haploid Lines

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

scholarly journals Involvement of homocastasterone, salicylic and abscisic acids in the regulation of drought and freezing tolerance in doubled haploid lines of winter barley

2019 ◽  
Vol 90 (1) ◽  
pp. 173-188 ◽  
Author(s):  
Sabina Malaga ◽  
Anna Janeczko ◽  
Franciszek Janowiak ◽  
Piotr Waligórski ◽  
Jana Oklestkova ◽  
...  
Keyword(s):  
Freezing Tolerance ◽  
Doubled Haploid ◽  
Plant Stress ◽  
Fold Increase ◽  
Winter Barley ◽  
Lower Amount ◽  
Stress Acclimation ◽  
Cereal Species ◽  
Dh Lines ◽  
First Time

Abstract Ten doubled haploid (DH) lines of winter barley with an increased range of freezing/drought tolerance were used to identify phytohormones involved in plant stress acclimation. Cold hardening and drought stress were applied at the most critical stages of plant development on young seedlings and heading plants, respectively. The level of the phytohormones was significantly higher at heading, more than 5-fold in respect of salicylic acid (SA) and total brassinosteroids (BRs) and 1.7-fold in the case of abscisic acid (ABA). Moreover, the spectrum of detectable BRs increased from one—homocastasterone (HCS)—found in seedlings to four BRs identified in heading plants [HCS, castasterone (CS), teasterone and dolicholide], with the last one detected for the first time in cereal species. To some extent freezing tolerance seems to be determined by native hormonal status as control seedlings of tolerant DH lines contained 1.4- and 2.3-fold lower amount of ABA and HCS and 2.3-fold higher amount of SA in comparison to freezing-sensitive ones. Such dependency was not observed in heading plants as significant variation in CS content was the only detected difference. Under stress treatments, tolerant DH lines accumulated significantly lower (75–81%) amount of ABA, which probably reflected lower stress intensity resulting from another defence strategy. In contrast, stress-induced significant almost 2-fold increase in HCS/CS and 2–3-fold decrease in SA content specific for tolerant DH lines of barley suggest the involvement of these molecules in freezing/drought defence. Detected correlations suggest their interaction with nonspecific peroxidase and low molecular weight antioxidants.

scholarly journals Agro-Physiologic Responses and Stress-Related Gene Expression of Four Doubled Haploid Wheat Lines under Salinity Stress Conditions

Biology ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 56
Author(s):  
Ibrahim Al-Ashkar ◽  
Walid Ben Romdhane ◽  
Rania A. El-Said ◽  
Abdelhalim Ghazy ◽  
Kotb Attia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Salinity Stress ◽  
Doubled Haploid ◽  
Stress Conditions ◽  
Path Coefficient ◽  
Related Gene ◽  
Salt Tolerant ◽  
Wheat Genotypes ◽  
Doubled Haploid Lines

Salinity majorly hinders horizontal and vertical expansion in worldwide wheat production. Productivity can be enhanced using salt-tolerant wheat genotypes. However, the assessment of salt tolerance potential in bread wheat doubled haploid lines (DHL) through agro-physiological traits and stress-related gene expression analysis could potentially minimize the cost of breeding programs and be a powerful way for the selection of the most salt-tolerant genotype. We used an extensive set of agro-physiologic parameters and salt-stress-related gene expressions. Multivariate analysis was used to detect phenotypic and genetic variations of wheat genotypes more closely under salinity stress, and we analyzed how these strategies effectively balance each other. Four doubled haploid lines (DHLs) and the check cultivar (Sakha93) were evaluated in two salinity levels (without and 150 mM NaCl) until harvest. The five genotypes showed reduced growth under 150 mM NaCl; however, the check cultivar (Sakha93) died at the beginning of the flowering stage. Salt stress induced reduction traits, except the canopy temperature and initial electrical conductivity, which was found in each of the five genotypes, with the greatest decline occurring in the check cultivar (Sakha-93) and the least decline in DHL2. The genotypes DHL21 and DHL5 exhibited increased expression rate of salt-stress-related genes (TaNHX1, TaHKT1, and TaCAT1) compared with DHL2 and Sakha93 under salt stress conditions. Principle component analysis detection of the first two components explains 70.78% of the overall variation of all traits (28 out of 32 traits). A multiple linear regression model and path coefficient analysis showed a coefficient of determination (R2) of 0.93. The models identified two interpretive variables, number of spikelets, and/or number of kernels, which can be unbiased traits for assessing wheat DHLs under salinity stress conditions, given their contribution and direct impact on the grain yield.

scholarly journals Candidate Genes for Freezing and Drought Tolerance Selected on the Basis of Proteome Analysis in Doubled Haploid Lines of Barley

2020 ◽  
Vol 21 (6) ◽  
pp. 2062
Author(s):  
Magdalena Wójcik-Jagła ◽  
Marcin Rapacz ◽  
Ewa Dubas ◽  
Monika Krzewska ◽  
Przemysław Kopeć ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
Doubled Haploid ◽  
Triosephosphate Isomerase ◽  
Elongation Factor ◽  
Proteome Analysis ◽  
Stress Factors ◽  
Plant Tolerance ◽  
Doubled Haploid Lines ◽  
Dh Lines

Plant tolerance to environmental stress is determined by a very complicated network composed of many intra- and extracellular factors. The aim of this study was to select candidate genes involved in responses to freezing and drought in barley on the basis of previous proteomic studies and to analyze changes in their expression caused by application of both stress factors. Six candidate genes for freezing tolerance (namely the genes encoding elongation factor 1 alpha (EF1A), ferredoxin-NADP reductase, a 14-3-3a protein, β-fructofuranosidase, CBF2A and CBF4B) and six for drought tolerance (encoding transketolase, periplasmic serine protease, triosephosphate isomerase, a protein with a co-chaperon region (GroEs), pfam14200 and actin) were chosen arbitrarily on the basis of in silico bioinformatic analyses. The expression levels of these genes were measured under control and stress conditions in six DH (doubled haploid) lines with differing freezing and drought tolerance. The results of gene expression analysis confirmed the roles of the candidate genes preselected in this study on the basis of previous proteome analysis in contributing to the differences in freezing and drought tolerance observed in the studied population of DH lines of winter barley.

scholarly journals Genetic Evaluation of Root Traits for Doubled Haploid Lines of Barely in Normal and Salt Stress Conditions

2017 ◽  
Vol 9 (21) ◽  
pp. 115-121
Author(s):  
Nasibeh Abdollah Zadeg ◽  
Reza Fotovat ◽  
Farid Shekari ◽  
Seyyed Mohammad Alavi Sini
Keyword(s):  
Salt Stress ◽  
Doubled Haploid ◽  
Root Traits ◽  
Genetic Evaluation ◽  
Stress Conditions ◽  
Doubled Haploid Lines

scholarly journals Content-Based Estimation of Brain MRI Tilt in Three Orthogonal Directions

2021 ◽  
Author(s):  
Pooja Prabhu ◽  
A. K. Karunakar ◽  
Sanjib Sinha ◽  
N. Mariyappa ◽  
G. K. Bhargava ◽  
...  
Keyword(s):  
Large Scale ◽  
Brain Mri ◽  
Similarity Measures ◽  
Principal Component ◽  
Brain Anatomy ◽  
Morphological Operations ◽  
Mr Images ◽  
Tilt Correction ◽  
Brain Mr Images ◽  
The Brain

AbstractIn a general scenario, the brain images acquired from magnetic resonance imaging (MRI) may experience tilt, distorting brain MR images. The tilt experienced by the brain MR images may result in misalignment during image registration for medical applications. Manually correcting (or estimating) the tilt on a large scale is time-consuming, expensive, and needs brain anatomy expertise. Thus, there is a need for an automatic way of performing tilt correction in three orthogonal directions (X, Y, Z). The proposed work aims to correct the tilt automatically by measuring the pitch angle, yaw angle, and roll angle in X-axis, Z-axis, and Y-axis, respectively. For correction of the tilt around the Z-axis (pointing to the superior direction), image processing techniques, principal component analysis, and similarity measures are used. Also, for correction of the tilt around the X-axis (pointing to the right direction), morphological operations, and tilt correction around the Y-axis (pointing to the anterior direction), orthogonal regression is used. The proposed approach was applied to adjust the tilt observed in the T1- and T2-weighted MR images. The simulation study with the proposed algorithm yielded an error of 0.40 ± 0.09°, and it outperformed the other existing studies. The tilt angle (in degrees) obtained is ranged from 6.2 ± 3.94, 2.35 ± 2.61, and 5 ± 4.36 in X-, Z-, and Y-directions, respectively, by using the proposed algorithm. The proposed work corrects the tilt more accurately and robustly when compared with existing studies.

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